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The map label for this gene is ilvE [H]

Identifier: 226948177

GI number: 226948177

Start: 1102115

End: 1103140

Strand: Direct

Name: ilvE [H]

Synonym: CLM_1051

Alternate gene names: 226948177

Gene position: 1102115-1103140 (Clockwise)

Preceding gene: 226948176

Following gene: 226948178

Centisome position: 26.52

GC content: 33.53

Gene sequence:

>1026_bases
ATGAATAAAGAAGTAAATATTGATTGGGGAAATCTAGGGTTTAATTATATAAATACAGATTTACGTTATACATCAAGATG
GAAAGATGGAAAATGGGATGAAGGTCAATTAGTTGAAGATAATATGATTAGTATAAATGAATCTTCAACTGCTCTTCACT
ATGGGCAACAATGCTTTGAAGGATTAAAAGCTTATCGTACAAAAGATAATAAAATTCAACTTTTTAGACCAGATAGAAAT
GCAGAACGTATGCAAGATAGTTGTAAACGTATTTTAATGCCGGAAGTTCCAACTGAAAAATTTATAGACGCATGTATTAA
AGTTGTTAAAGCAAATGAGCATTATGTGCCACCTTATGGTAGTGGAGCAACGTTGTATCTTAGACCTTTTGTAATAGGTG
TTGGAGACAATATTGGTGTTAAGCCGGCAGCTGAATATATATTTTCTATATTCTGTATACCAGTAGGACCTTATTTTAAA
GGTGGCATGGTGCCAGTAAACTTTACAGTATCAGATTATGATAGAGCAGCTCCATACGGAACCGGTGCAGTAAAGGTTGG
TGGTAACTATGCTGGAAGTCTTTATCCACATGAATTAGCTGTGAAAAAAGGATTTGCAGATTGTATTTATCTCGATCCAG
CTACTCACACCAAAATAGAAGAGGTTGGAGCAGCTAACTTCTTTGGAATAACAAAGGATAATAAATTTATTACTCCAAAA
TCTCCATCAATTCTTCCAAGTATAACTAAGTATTCCCTTCTTCATATTGCAAAAGAATATTTAAAGATGGAAGTTGAAGA
AAGAGATGTTTTAATTGATAATTTAGATGAATTTAAAGAAGCTGGTGCTTGTGGAACTGCTGCAGTAATAACTCCAATTG
GTGGAATAGAATATAAAGGAAAATTACGTGTATTCCATAGTGAAACAGAAGTAGGACCTATAACCAAAAAACTTTACGAT
ACACTTTATGGAATTCAATTTGGAGAGGTAGAAGCTCCACAGGGATGGATTGTAGAAGTTAAATAA

Upstream 100 bases:

>100_bases
ATAAAATGAATATTTATGAAAGATTTTAAATAAGGATACTAGGAATTATAGTTGCAGTAAAAGCCTTAGAAAAAATATTT
TTAGTTTGGAGGTACTTTGA

Downstream 100 bases:

>100_bases
TAGGTTACAAAATTTCATAATTATAATTAAGATAAATCAAGTTTAAATAGCATTAAAATATAAATTAATTGTTATATGTA
TAAATTTTAGCTAAAATAAA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNKEVNIDWGNLGFNYINTDLRYTSRWKDGKWDEGQLVEDNMISINESSTALHYGQQCFEGLKAYRTKDNKIQLFRPDRN
AERMQDSCKRILMPEVPTEKFIDACIKVVKANEHYVPPYGSGATLYLRPFVIGVGDNIGVKPAAEYIFSIFCIPVGPYFK
GGMVPVNFTVSDYDRAAPYGTGAVKVGGNYAGSLYPHELAVKKGFADCIYLDPATHTKIEEVGAANFFGITKDNKFITPK
SPSILPSITKYSLLHIAKEYLKMEVEERDVLIDNLDEFKEAGACGTAAVITPIGGIEYKGKLRVFHSETEVGPITKKLYD
TLYGIQFGEVEAPQGWIVEVK

Sequences:

>Translated_341_residues
MNKEVNIDWGNLGFNYINTDLRYTSRWKDGKWDEGQLVEDNMISINESSTALHYGQQCFEGLKAYRTKDNKIQLFRPDRN
AERMQDSCKRILMPEVPTEKFIDACIKVVKANEHYVPPYGSGATLYLRPFVIGVGDNIGVKPAAEYIFSIFCIPVGPYFK
GGMVPVNFTVSDYDRAAPYGTGAVKVGGNYAGSLYPHELAVKKGFADCIYLDPATHTKIEEVGAANFFGITKDNKFITPK
SPSILPSITKYSLLHIAKEYLKMEVEERDVLIDNLDEFKEAGACGTAAVITPIGGIEYKGKLRVFHSETEVGPITKKLYD
TLYGIQFGEVEAPQGWIVEVK
>Mature_341_residues
MNKEVNIDWGNLGFNYINTDLRYTSRWKDGKWDEGQLVEDNMISINESSTALHYGQQCFEGLKAYRTKDNKIQLFRPDRN
AERMQDSCKRILMPEVPTEKFIDACIKVVKANEHYVPPYGSGATLYLRPFVIGVGDNIGVKPAAEYIFSIFCIPVGPYFK
GGMVPVNFTVSDYDRAAPYGTGAVKVGGNYAGSLYPHELAVKKGFADCIYLDPATHTKIEEVGAANFFGITKDNKFITPK
SPSILPSITKYSLLHIAKEYLKMEVEERDVLIDNLDEFKEAGACGTAAVITPIGGIEYKGKLRVFHSETEVGPITKKLYD
TLYGIQFGEVEAPQGWIVEVK

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=341, Percent_Identity=34.6041055718475, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI38176287, Length=341, Percent_Identity=34.6041055718475, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI296010906, Length=341, Percent_Identity=34.6041055718475, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI50658084, Length=342, Percent_Identity=31.8713450292398, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI258614015, Length=297, Percent_Identity=32.996632996633, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI296010902, Length=341, Percent_Identity=29.0322580645161, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI296010900, Length=341, Percent_Identity=29.0322580645161, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI48994963, Length=324, Percent_Identity=30.5555555555556, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17568601, Length=357, Percent_Identity=30.812324929972, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17565728, Length=358, Percent_Identity=29.3296089385475, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322608, Length=333, Percent_Identity=35.1351351351351, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6322002, Length=340, Percent_Identity=33.8235294117647, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24641779, Length=343, Percent_Identity=29.7376093294461, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 38089; Mature: 38089

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEVNIDWGNLGFNYINTDLRYTSRWKDGKWDEGQLVEDNMISINESSTALHYGQQCFE
CCCEEEEEECCCCCEEEECCCEEECCCCCCCCCCCCEECCCEEEECCCCHHHHHHHHHHH
GLKAYRTKDNKIQLFRPDRNAERMQDSCKRILMPEVPTEKFIDACIKVVKANEHYVPPYG
HHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
SGATLYLRPFVIGVGDNIGVKPAAEYIFSIFCIPVGPYFKGGMVPVNFTVSDYDRAAPYG
CCCEEEEEEEEEECCCCCCCCHHHHHHHEEHEEECCCCCCCCEEEEEEEECCCCCCCCCC
TGAVKVGGNYAGSLYPHELAVKKGFADCIYLDPATHTKIEEVGAANFFGITKDNKFITPK
CCEEEECCCCCCCCCCHHHHHHCCCCCEEEECCCCCCHHHHCCCCCEEEECCCCCEECCC
SPSILPSITKYSLLHIAKEYLKMEVEERDVLIDNLDEFKEAGACGTAAVITPIGGIEYKG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCCEEECC
KLRVFHSETEVGPITKKLYDTLYGIQFGEVEAPQGWIVEVK
EEEEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCEEEEEC
>Mature Secondary Structure
MNKEVNIDWGNLGFNYINTDLRYTSRWKDGKWDEGQLVEDNMISINESSTALHYGQQCFE
CCCEEEEEECCCCCEEEECCCEEECCCCCCCCCCCCEECCCEEEECCCCHHHHHHHHHHH
GLKAYRTKDNKIQLFRPDRNAERMQDSCKRILMPEVPTEKFIDACIKVVKANEHYVPPYG
HHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
SGATLYLRPFVIGVGDNIGVKPAAEYIFSIFCIPVGPYFKGGMVPVNFTVSDYDRAAPYG
CCCEEEEEEEEEECCCCCCCCHHHHHHHEEHEEECCCCCCCCEEEEEEEECCCCCCCCCC
TGAVKVGGNYAGSLYPHELAVKKGFADCIYLDPATHTKIEEVGAANFFGITKDNKFITPK
CCEEEECCCCCCCCCCHHHHHHCCCCCEEEECCCCCCHHHHCCCCCEEEECCCCCEECCC
SPSILPSITKYSLLHIAKEYLKMEVEERDVLIDNLDEFKEAGACGTAAVITPIGGIEYKG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCCEEECC
KLRVFHSETEVGPITKKLYDTLYGIQFGEVEAPQGWIVEVK
EEEEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]