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The map label for this gene is thiD [H]

Identifier: 226948060

GI number: 226948060

Start: 979382

End: 980209

Strand: Direct

Name: thiD [H]

Synonym: CLM_0921

Alternate gene names: 226948060

Gene position: 979382-980209 (Clockwise)

Preceding gene: 226948059

Following gene: 226948061

Centisome position: 23.57

GC content: 32.97

Gene sequence:

>828_bases
ATGAAGAAAGTACTTAGTATTGCAGGTTCTGACTGTAGTGGAGGGGCAGGAATACAAGCTGATTTAAAGACTTTTTCCGC
TCATGGGGTTTTCGGAATGAGTGTAATTGTATCTGTAGTTGCAGAAAATACCAGCAGAGTAATTGATATTCAAGATATTA
CACATGATATGATTAAAAGTCAGATTGATGCAGTTTATGAGGATATTGGTACGGATGCAGTAAAAATAGGAATGCTATCT
ACACCAGATTGTATGAAAGCAGTATCTGAAAAATTACAAGAATATAAGCCGAAAAATGTTGTAATAGATCCAGTTATGTA
TGCAAAAAATGGGTGCCCTTTAATGGACCCTAATTCCGTATCTACACTAATTGAATCTATTATTTGTCATGCAGATATTT
TGACTCCCAATATACCAGAGGCAGAGAGAATTTCAGGGTACAAAATAAAAACTTTAGAAGATATGGAGAAAGCTGCTGGC
ATTATTGAAGGCATGGGATGTAAAAGTGTTTTAATAAAAGGTGGACATTATATTGGGGATGCTGTAGATATATTATATGA
TGGGAATAAATTTTATCATTATAAAACTCAGAGAATCCATACAAAAAATACACATGGAACAGGTTGCACATTATCTTCTG
CAATTGCTTCTAATTTAGCTTTAGGTTTCGAAATAAATGAAGCGGTAAGAAGAGCAAAAAATTATATAACTATGGCAATC
GAACATTCATTAGAAATTGGTAAAGGAAATGGACCAACTAACCATTTTTATCAAGTTTACTTAAATGGACTAAAGGGAAT
GGAATTAGATAATCATACAAGAAGTTAA

Upstream 100 bases:

>100_bases
TTACTGAATATTTACAACAATGATATAATAAATAATAATCATTGAAATTAAAGGTACTTTTATTATTAAAAGTGCCTTTT
TGTAGAAGGGAGAAAAATTT

Downstream 100 bases:

>100_bases
AACCCTTTATTAGAATAAATGATTTAAAAGCTATGAATTAGAAATTTATAAAAAATTATAAGGAACAGTATTTGAAAATC
TATTGAAATTTGGTACATCT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKKVLSIAGSDCSGGAGIQADLKTFSAHGVFGMSVIVSVVAENTSRVIDIQDITHDMIKSQIDAVYEDIGTDAVKIGMLS
TPDCMKAVSEKLQEYKPKNVVIDPVMYAKNGCPLMDPNSVSTLIESIICHADILTPNIPEAERISGYKIKTLEDMEKAAG
IIEGMGCKSVLIKGGHYIGDAVDILYDGNKFYHYKTQRIHTKNTHGTGCTLSSAIASNLALGFEINEAVRRAKNYITMAI
EHSLEIGKGNGPTNHFYQVYLNGLKGMELDNHTRS

Sequences:

>Translated_275_residues
MKKVLSIAGSDCSGGAGIQADLKTFSAHGVFGMSVIVSVVAENTSRVIDIQDITHDMIKSQIDAVYEDIGTDAVKIGMLS
TPDCMKAVSEKLQEYKPKNVVIDPVMYAKNGCPLMDPNSVSTLIESIICHADILTPNIPEAERISGYKIKTLEDMEKAAG
IIEGMGCKSVLIKGGHYIGDAVDILYDGNKFYHYKTQRIHTKNTHGTGCTLSSAIASNLALGFEINEAVRRAKNYITMAI
EHSLEIGKGNGPTNHFYQVYLNGLKGMELDNHTRS
>Mature_275_residues
MKKVLSIAGSDCSGGAGIQADLKTFSAHGVFGMSVIVSVVAENTSRVIDIQDITHDMIKSQIDAVYEDIGTDAVKIGMLS
TPDCMKAVSEKLQEYKPKNVVIDPVMYAKNGCPLMDPNSVSTLIESIICHADILTPNIPEAERISGYKIKTLEDMEKAAG
IIEGMGCKSVLIKGGHYIGDAVDILYDGNKFYHYKTQRIHTKNTHGTGCTLSSAIASNLALGFEINEAVRRAKNYITMAI
EHSLEIGKGNGPTNHFYQVYLNGLKGMELDNHTRS

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=266, Percent_Identity=42.4812030075188, Blast_Score=218, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6325378, Length=276, Percent_Identity=38.0434782608696, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6324997, Length=277, Percent_Identity=37.5451263537906, Blast_Score=145, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6324517, Length=277, Percent_Identity=36.8231046931408, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29845; Mature: 29845

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLSIAGSDCSGGAGIQADLKTFSAHGVFGMSVIVSVVAENTSRVIDIQDITHDMIKS
CCCEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
QIDAVYEDIGTDAVKIGMLSTPDCMKAVSEKLQEYKPKNVVIDPVMYAKNGCPLMDPNSV
HHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEECCHHCCCCCCCCCCCCHH
STLIESIICHADILTPNIPEAERISGYKIKTLEDMEKAAGIIEGMGCKSVLIKGGHYIGD
HHHHHHHHHHHHHCCCCCCCHHHCCCEEECHHHHHHHHHHHHHCCCHHHHEEECCCCCCC
AVDILYDGNKFYHYKTQRIHTKNTHGTGCTLSSAIASNLALGFEINEAVRRAKNYITMAI
EEEEEECCCEEEEEEECEEECCCCCCCCCCHHHHHHHHHEECEEHHHHHHHHHHHEEEEE
EHSLEIGKGNGPTNHFYQVYLNGLKGMELDNHTRS
EECEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKKVLSIAGSDCSGGAGIQADLKTFSAHGVFGMSVIVSVVAENTSRVIDIQDITHDMIKS
CCCEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
QIDAVYEDIGTDAVKIGMLSTPDCMKAVSEKLQEYKPKNVVIDPVMYAKNGCPLMDPNSV
HHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEECCHHCCCCCCCCCCCCHH
STLIESIICHADILTPNIPEAERISGYKIKTLEDMEKAAGIIEGMGCKSVLIKGGHYIGD
HHHHHHHHHHHHHCCCCCCCHHHCCCEEECHHHHHHHHHHHHHCCCHHHHEEECCCCCCC
AVDILYDGNKFYHYKTQRIHTKNTHGTGCTLSSAIASNLALGFEINEAVRRAKNYITMAI
EEEEEECCCEEEEEEECEEECCCCCCCCCCHHHHHHHHHEECEEHHHHHHHHHHHEEEEE
EHSLEIGKGNGPTNHFYQVYLNGLKGMELDNHTRS
EECEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA