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The map label for this gene is mscL [H]

Identifier: 226947806

GI number: 226947806

Start: 713456

End: 713857

Strand: Direct

Name: mscL [H]

Synonym: CLM_0656

Alternate gene names: 226947806

Gene position: 713456-713857 (Clockwise)

Preceding gene: 226947804

Following gene: 226947807

Centisome position: 17.17

GC content: 24.88

Gene sequence:

>402_bases
TTGAAAAATATAATTGAAGAATTTAAAGACTTTGCAGTAAAAGGAAATGCTGTAGAATTAGCAGTAGGTGTGGTTATAGG
TGGAGCTTTTGGTAAAATAATAACTTCTTTAGTAGAGGATATTATAATGCCTTTAGTAGGATTGTTAATAGGTGGTATAG
ATTTTACTAATTTAAAATTTACAATGAGCTTTTCAGAAAAAACAGTAGTTTCTATAAAATACGGTAATTTTATACAAGCG
GCAGTAAACTTTTTAATAATTTCCTTTTCTATTTTTTTGTTTATTAAATTAATAAATAAGTTCAAGAAAAAGGAAGAGGA
AGTAAAAGAAGAACCTAAAATAAGTAATGAAGAAATATTATTAACAGAAATAAGGGATTTGTTAAAGGAAAATAAATCCT
AA

Upstream 100 bases:

>100_bases
AACAATACATGAATATTTTACCAGAATAAAATTCTTGAAAATAAATGATCTTTATTATAATATACATAATAGTTTATAGT
TAAATATGGAGGTTTTAATT

Downstream 100 bases:

>100_bases
TAATTTATTTATGATGTGAAGGTTTTCTTTGTTGACACTTATAATTATTAGTAATAAAATTATTATGTAAATAAAATTAA
GTGCTAGGGGTGCCAAAAGT

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MKNIIEEFKDFAVKGNAVELAVGVVIGGAFGKIITSLVEDIIMPLVGLLIGGIDFTNLKFTMSFSEKTVVSIKYGNFIQA
AVNFLIISFSIFLFIKLINKFKKKEEEVKEEPKISNEEILLTEIRDLLKENKS

Sequences:

>Translated_133_residues
MKNIIEEFKDFAVKGNAVELAVGVVIGGAFGKIITSLVEDIIMPLVGLLIGGIDFTNLKFTMSFSEKTVVSIKYGNFIQA
AVNFLIISFSIFLFIKLINKFKKKEEEVKEEPKISNEEILLTEIRDLLKENKS
>Mature_133_residues
MKNIIEEFKDFAVKGNAVELAVGVVIGGAFGKIITSLVEDIIMPLVGLLIGGIDFTNLKFTMSFSEKTVVSIKYGNFIQA
AVNFLIISFSIFLFIKLINKFKKKEEEVKEEPKISNEEILLTEIRDLLKENKS

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=135, Percent_Identity=56.2962962962963, Blast_Score=147, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14905; Mature: 14905

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIIEEFKDFAVKGNAVELAVGVVIGGAFGKIITSLVEDIIMPLVGLLIGGIDFTNLKF
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
TMSFSEKTVVSIKYGNFIQAAVNFLIISFSIFLFIKLINKFKKKEEEVKEEPKISNEEIL
EEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
LTEIRDLLKENKS
HHHHHHHHHCCCC
>Mature Secondary Structure
MKNIIEEFKDFAVKGNAVELAVGVVIGGAFGKIITSLVEDIIMPLVGLLIGGIDFTNLKF
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
TMSFSEKTVVSIKYGNFIQAAVNFLIISFSIFLFIKLINKFKKKEEEVKEEPKISNEEIL
EEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
LTEIRDLLKENKS
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9922257 [H]