Click here to switch to the map view.

The map label for this gene is ubiE [H]

Identifier: 226947704

GI number: 226947704

Start: 596669

End: 597259

Strand: Direct

Name: ubiE [H]

Synonym: CLM_0551

Alternate gene names: 226947704

Gene position: 596669-597259 (Clockwise)

Preceding gene: 226947703

Following gene: 226947707

Centisome position: 14.36

GC content: 26.57

Gene sequence:

>591_bases
ATGAATGATAAAGAGTTTTTTAATAATTTAGCAGATAAGTGGGACGATATGTGTTTTCATTCAAAAGAGAAAATAGAATA
TATTCTTTCAAAAGCAAATTTGAAAGAAGGAGATAGAGTTTTAGATATAGGTAGTGGTACAGGAGTACTTATACCTTATT
TAGAAAATATTATTTCTAATAATGGTAATATAGCTGCTATAGATATAGCAGAAAATATGTTAAAGGTATCAAAAGAAAAA
AACAAATACTCTAATTTAAAGTTTATAGTAGGAGATTTTTTAGAGTATAAATCTAAAAACACTTTTAATTGCATAACAGC
ATATTCTTGTTATCCTCATTTTAAGGACAAGGATAAATTAGCACATAGAGCCTATGAACTACTTGAGGAAGGTGGTAGAT
TAGTTATAGCTCATAGTGAAAGTAAAGAGAAGATAAACTCTAGACATAAAGATGTAGATCATAAGATAAAGTCACATATA
TTGCCTAAGGTGAAATATACTGCAGAATTAATGGAAAAACATAATTTTAAAACTATATATACAGAGGATAATGAAGACTG
TTATATTTATATAGGTGCAAGAAACAAATAA

Upstream 100 bases:

>100_bases
TTATTTGTAAAAGGTTTGCCGGGTATAGTTATTCAATTAATACTTATACCTATATTAGTTAAATCATTAGGGAAAGTAGA
ATTAGGAAAGGCTGTATAAT

Downstream 100 bases:

>100_bases
TTTGATTAGATAAATTTTATGTATGATTAACCAAAATAAAAGTGTGGAGTAAAAATATAAAAACTACTCCACACTTTTAC
TTTATTTAATTTATTTCATC

Product: methlytransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MNDKEFFNNLADKWDDMCFHSKEKIEYILSKANLKEGDRVLDIGSGTGVLIPYLENIISNNGNIAAIDIAENMLKVSKEK
NKYSNLKFIVGDFLEYKSKNTFNCITAYSCYPHFKDKDKLAHRAYELLEEGGRLVIAHSESKEKINSRHKDVDHKIKSHI
LPKVKYTAELMEKHNFKTIYTEDNEDCYIYIGARNK

Sequences:

>Translated_196_residues
MNDKEFFNNLADKWDDMCFHSKEKIEYILSKANLKEGDRVLDIGSGTGVLIPYLENIISNNGNIAAIDIAENMLKVSKEK
NKYSNLKFIVGDFLEYKSKNTFNCITAYSCYPHFKDKDKLAHRAYELLEEGGRLVIAHSESKEKINSRHKDVDHKIKSHI
LPKVKYTAELMEKHNFKTIYTEDNEDCYIYIGARNK
>Mature_196_residues
MNDKEFFNNLADKWDDMCFHSKEKIEYILSKANLKEGDRVLDIGSGTGVLIPYLENIISNNGNIAAIDIAENMLKVSKEK
NKYSNLKFIVGDFLEYKSKNTFNCITAYSCYPHFKDKDKLAHRAYELLEEGGRLVIAHSESKEKINSRHKDVDHKIKSHI
LPKVKYTAELMEKHNFKTIYTEDNEDCYIYIGARNK

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Escherichia coli, GI48994983, Length=191, Percent_Identity=28.2722513089005, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 22737; Mature: 22737

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKEFFNNLADKWDDMCFHSKEKIEYILSKANLKEGDRVLDIGSGTGVLIPYLENIISN
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCC
NGNIAAIDIAENMLKVSKEKNKYSNLKFIVGDFLEYKSKNTFNCITAYSCYPHFKDKDKL
CCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHH
AHRAYELLEEGGRLVIAHSESKEKINSRHKDVDHKIKSHILPKVKYTAELMEKHNFKTIY
HHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEE
TEDNEDCYIYIGARNK
ECCCCCEEEEEECCCC
>Mature Secondary Structure
MNDKEFFNNLADKWDDMCFHSKEKIEYILSKANLKEGDRVLDIGSGTGVLIPYLENIISN
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCC
NGNIAAIDIAENMLKVSKEKNKYSNLKFIVGDFLEYKSKNTFNCITAYSCYPHFKDKDKL
CCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHH
AHRAYELLEEGGRLVIAHSESKEKINSRHKDVDHKIKSHILPKVKYTAELMEKHNFKTIY
HHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEE
TEDNEDCYIYIGARNK
ECCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA