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The map label for this gene is fliY [H]

Identifier: 226947682

GI number: 226947682

Start: 574829

End: 575632

Strand: Reverse

Name: fliY [H]

Synonym: CLM_0528

Alternate gene names: 226947682

Gene position: 575632-574829 (Counterclockwise)

Preceding gene: 226947684

Following gene: 226947681

Centisome position: 13.85

GC content: 28.36

Gene sequence:

>804_bases
ATGAAGAAAAAAAATATATTATTTTCAATTTTAATAATGGGATTAATTTTTATGCTGACAGGTTGTGATAACAAATCAAA
AGAAGATACTTCCCTTAAGGATATAAAAGCCAAAGGTGAATTTGTCGTAGGTTTAGACGATAGCTTCCCTCCTATGGGAT
TTAAAAATGATAAAGGTGAAATAGTCGGATTTGATATTGATTTAGCAAAAGAAGTTGCTAAAAAAATGGGAGTTAAAGTT
GTATTTAAACCAGTTCAGTGGGATGGTGTAGTTCTTAGTTTAAATAACAAGGATATAGATGTTATCTGGAATGGACTTAC
AATAACTGAAAAAAGAAAAGAACAAATAAATTTTTCAAAACCTTATGTAGAAAACAAACAAATAATAGTGGTTAATAATG
ACTCTAATATAAAATCTAAAAAAGATTTAAATAAAAAAACTGTAGCTATACAATTAGGTAGTAGTAGTGAAGTTGCTTTA
GATTCAGATAAAGACTTTGTGAAATCATTAAAGGAATTAAAAAAATACTCAAATAACACTGAAGCACTTATGGATTTGCA
AGCAAAAAGAGTTGATGCCGTTGTAGTAGATGAGGTAGTTGGTAGATATTATATTAGCAAAAAACCTAACGTATTCAAAG
TATTAGATGAAGACTTTGGGGGAGAATCTTATGGGATAGGTTTTAGAAAAACTGACAATAGTTTTAGAGAAGCAGTAGAT
AAAGCTTTAGATGAAGTTATAAAAGATGGAACAGCAGATAAAATATCTGAAAAATGGTTTGGTAAAGGAATCTTAAAAAA
ATAG

Upstream 100 bases:

>100_bases
TAATTTTAAATTATGTGATAAGATTTTTTGATTTTATCACTTTACAAATTCACCACACTAAAGTATAATGTAGTAAAATA
AAACAAGGGGGATTTTTTAT

Downstream 100 bases:

>100_bases
GATGTGATAAATGTGTCTAATCTTTCAGATATAACTATATTTATATTAAAAGGTAGTATTATTTCTTTTAAATTATTCAT
AGTAACAATAATATTTTCTA

Product: polar amino acid uptake ABC transporter amino acid-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKKKNILFSILIMGLIFMLTGCDNKSKEDTSLKDIKAKGEFVVGLDDSFPPMGFKNDKGEIVGFDIDLAKEVAKKMGVKV
VFKPVQWDGVVLSLNNKDIDVIWNGLTITEKRKEQINFSKPYVENKQIIVVNNDSNIKSKKDLNKKTVAIQLGSSSEVAL
DSDKDFVKSLKELKKYSNNTEALMDLQAKRVDAVVVDEVVGRYYISKKPNVFKVLDEDFGGESYGIGFRKTDNSFREAVD
KALDEVIKDGTADKISEKWFGKGILKK

Sequences:

>Translated_267_residues
MKKKNILFSILIMGLIFMLTGCDNKSKEDTSLKDIKAKGEFVVGLDDSFPPMGFKNDKGEIVGFDIDLAKEVAKKMGVKV
VFKPVQWDGVVLSLNNKDIDVIWNGLTITEKRKEQINFSKPYVENKQIIVVNNDSNIKSKKDLNKKTVAIQLGSSSEVAL
DSDKDFVKSLKELKKYSNNTEALMDLQAKRVDAVVVDEVVGRYYISKKPNVFKVLDEDFGGESYGIGFRKTDNSFREAVD
KALDEVIKDGTADKISEKWFGKGILKK
>Mature_267_residues
MKKKNILFSILIMGLIFMLTGCDNKSKEDTSLKDIKAKGEFVVGLDDSFPPMGFKNDKGEIVGFDIDLAKEVAKKMGVKV
VFKPVQWDGVVLSLNNKDIDVIWNGLTITEKRKEQINFSKPYVENKQIIVVNNDSNIKSKKDLNKKTVAIQLGSSSEVAL
DSDKDFVKSLKELKKYSNNTEALMDLQAKRVDAVVVDEVVGRYYISKKPNVFKVLDEDFGGESYGIGFRKTDNSFREAVD
KALDEVIKDGTADKISEKWFGKGILKK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=260, Percent_Identity=33.0769230769231, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1788648, Length=226, Percent_Identity=34.5132743362832, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787031, Length=224, Percent_Identity=34.8214285714286, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788649, Length=267, Percent_Identity=32.5842696629214, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1787085, Length=221, Percent_Identity=30.316742081448, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787088, Length=219, Percent_Identity=28.7671232876712, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786876, Length=247, Percent_Identity=23.4817813765182, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29963; Mature: 29963

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKNILFSILIMGLIFMLTGCDNKSKEDTSLKDIKAKGEFVVGLDDSFPPMGFKNDKGE
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCC
IVGFDIDLAKEVAKKMGVKVVFKPVQWDGVVLSLNNKDIDVIWNGLTITEKRKEQINFSK
EEEEEHHHHHHHHHHCCEEEEEEEECCCCEEEEECCCCEEEEECCEEECCHHHHHCCCCC
PYVENKQIIVVNNDSNIKSKKDLNKKTVAIQLGSSSEVALDSDKDFVKSLKELKKYSNNT
CCCCCCEEEEEECCCCCCHHHCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHCCCH
EALMDLQAKRVDAVVVDEVVGRYYISKKPNVFKVLDEDFGGESYGIGFRKTDNSFREAVD
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCEECCCEEECCHHHHHHHH
KALDEVIKDGTADKISEKWFGKGILKK
HHHHHHHHCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKKNILFSILIMGLIFMLTGCDNKSKEDTSLKDIKAKGEFVVGLDDSFPPMGFKNDKGE
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCC
IVGFDIDLAKEVAKKMGVKVVFKPVQWDGVVLSLNNKDIDVIWNGLTITEKRKEQINFSK
EEEEEHHHHHHHHHHCCEEEEEEEECCCCEEEEECCCCEEEEECCEEECCHHHHHCCCCC
PYVENKQIIVVNNDSNIKSKKDLNKKTVAIQLGSSSEVALDSDKDFVKSLKELKKYSNNT
CCCCCCEEEEEECCCCCCHHHCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHCCCH
EALMDLQAKRVDAVVVDEVVGRYYISKKPNVFKVLDEDFGGESYGIGFRKTDNSFREAVD
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCEECCCEEECCHHHHHHHH
KALDEVIKDGTADKISEKWFGKGILKK
HHHHHHHHCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]