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The map label for this gene is adhE [H]

Identifier: 226947576

GI number: 226947576

Start: 434540

End: 437128

Strand: Direct

Name: adhE [H]

Synonym: CLM_0413

Alternate gene names: 226947576

Gene position: 434540-437128 (Clockwise)

Preceding gene: 226947575

Following gene: 226947577

Centisome position: 10.46

GC content: 32.52

Gene sequence:

>2589_bases
ATGAAAATTACCACTACTGAGGAACTAATAAGAAAAATTGAAAAAATCAAAGAGGCACAAAAGATTTATTCCACATATTC
TCAAGATAAGGTAGACAAAATATTTAAAGCAGCAGCTATAGCAGCTAACAAAGAAAGAATAAAACTTGCAAAAATGGCAG
TAGAAGAAACTGGCATGGGTATTGTAGAAGATAAAGTTATAAAAAATCATTTTGCATCTGAATATATTTATAACAAATAT
AAAGATGAAAAAACCTGTGGTGTAATAGAAAAGGATGAAGCCTTTGGGTTAACAAAAATTGCAGAACCAATAGGTGTTAT
TGCGGCAATAGTTCCAACAACTAATCCAACCTCAACAGCTATTTTCAAAGCTTTAATAGCTTTAAAAACTAGAAATGGTA
TAATTTTTTCACCTCATCCAAGAGCTAAAAAATCTACAATAATGGCAGCTAAGATTGTTTTAGATGCAGCAGTACAAGCA
GGGGCACCAAAAGAAATAATAGGTTGGATAGATGAACCTACCTTAGAATTGTCTAATGCAGTAATGAGTAATTCAAATCT
AGTTCTTGCAACAGGTGGACCAGGTATGGTTAAAGCGGCATACTCTTCAGGAAAGCCAGCTATAGGAGTAGGACCTGGAA
ATGTACCAGCTATAATATACGAAACAGCAGATATTAAAATGGCTGTAAGCTCAGTGGTACTTTCTAAAACTTTTGATAAT
GGTATGATTTGTGCATCAGAACAATCAGTAATTGTGATGAATAGCATATATGAAGAAGTAAAAAAAGAATTTGTAATTAG
AGGAGCCTATGTATTAAATAAAGAAGAGATAGAAAAAGTTAAGAAAATAATTTTAGTTAATGGAAATGTAAATGCTAAAA
TTGTTGGTCAAACACCACAAAAGATAGGTGAAATGGCTGGAGTAAAAGTTCCAGATTGGGCTAAACTTTTAGTTGGAGAA
GTTCAATCTGTAGAATTAGAAGAACCATTCTCTCACGAAAAACTATCACCAGTTTTAGCAATGTATAAAGTTAAAACTTA
TGAAGAGGCTTTAACTAAAGCTGAAAGATTAGTAGAATTAGGAGGATTTGGACATACATCTTCATTGTATATAAACACAG
TAAAATGCAAAGAAGAAGTAGAAAAGTTCTCAAATAATATGAAAACAGGAAGAACAATTATAAATATGCCATCAGCCCAA
GGTGGTATAGGTGATATATATAACTTTAAATTAGCACCATCTCTAACTCTTGGTTGCGGATCCTGGGGTGGAAACTCAGT
ATCAGAGAATGTAGGACCAAAACACTTATTAAATATCAAAAATGTAGCTGAGAGGAGAGAGAATATGCTTTGGTTTAGAG
TACCAGAAAAAGTTTACTTTAAATACGGATGTCTTCCAATAGCTTTAAAAGAATTAAAAAGAATGAATAAGAAAAAGGCA
TTTATAGTTACAGATAAAGTATTATATGAATTAGGAGTTGCTAAAAAAGCAACAGATGTGTTAGATGAGATAGGAATAAA
TTATAAGGTATTCTTTGATGTAGCGCCAGATCCAACTTTAGAAACAGCAAAAAAAGGTGCAAAAGAAATGGTAGATTTTA
ATCCTGATACAATAATAGCTATAGGCGGAGGATCTGCTATGGATGCTGCTAAAATAATGTGGGTAATGTATGAACATCCA
GAAGCAGAATTCGAAGATTTAGCTATGAGATTTATGGATATAAGAAAAAGAGTATATGAGTTCCCACATATGGGAGATAA
AGCAATGATGATATCAGTAGCTACTTCAGCAGGTACTGGTTCAGAAGTAACTCCTTTTGCTGTTATAACTGATGAAAAAA
CAGGTGTGAAATATCCACTAGCAGATTATGAATTAACTCCAGATATGGCTATAGTAGATGCAGATTTAATGCTTAATATG
CCAAAGGGATTAACTGCTGCATCAGGAATTGATGCATTAACTCATGCAGTAGAAGCTTATGTATCAGTTATGGCATCAGA
GTATACAGATGGACTATGTTTAGAGGCTATAAAAACCATATTTGAATATCTACCAAAAGCTTATAAAGAAGGAGCCCAAG
ATATAGAAGCAAGAGAAAAAATGGCTCATGCATCAACTATAGCAGGTATGGCTTTTGCAAATGCCTTCTTAGGAGTATGC
CACTCTATGGCTCATAAATTAGGATCTATGCATCATGTACCACATGGTATAGCTAATGCTTTATTAATAAATGAAACAAT
TAAATTTAATTCTGAGGATATGCCAAGAAAGCAAACTGCTTTCCCACAATATAAATATCCAAATGCTAAGGCTAAATATG
CTAATATAGCAGATTATTTATCATTAGGTGGAAAAACTCCAGAAGAAAAAGTTGAGCTATTAATAAAAGCTATAGATAAA
TTAAAAGCAGAAGTAAATATTCCAACCTCTATAGAAGAGGCAGGGATATCAAAGGATAAATTCTTTAAAACTTTAGATGA
GATGTCCGAACAAGCTTTTGATGATCAATGTACAGGAGCTAATCCAAGATACCCATTAATAAGTGAAATAAAACAAATGT
ATACTAATGTTTTTAGTACTAAAAAATAG

Upstream 100 bases:

>100_bases
AGAAGTTATAAGGTATTAGTAATATTAATATAAATTAATACTTTTTATATTTTAGATAAGTTTGTTAAAAAATAAACTAT
TATTAAAAGAGGTGTTATTA

Downstream 100 bases:

>100_bases
GATAGTTTAATTTATATAGCATAAAATATTATTATATATATTTTATAGAAAGCTAAAATTTTAATTTTACCCAAAACTAA
ACCATTTATACAAAAAATAA

Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH [H]

Number of amino acids: Translated: 862; Mature: 862

Protein sequence:

>862_residues
MKITTTEELIRKIEKIKEAQKIYSTYSQDKVDKIFKAAAIAANKERIKLAKMAVEETGMGIVEDKVIKNHFASEYIYNKY
KDEKTCGVIEKDEAFGLTKIAEPIGVIAAIVPTTNPTSTAIFKALIALKTRNGIIFSPHPRAKKSTIMAAKIVLDAAVQA
GAPKEIIGWIDEPTLELSNAVMSNSNLVLATGGPGMVKAAYSSGKPAIGVGPGNVPAIIYETADIKMAVSSVVLSKTFDN
GMICASEQSVIVMNSIYEEVKKEFVIRGAYVLNKEEIEKVKKIILVNGNVNAKIVGQTPQKIGEMAGVKVPDWAKLLVGE
VQSVELEEPFSHEKLSPVLAMYKVKTYEEALTKAERLVELGGFGHTSSLYINTVKCKEEVEKFSNNMKTGRTIINMPSAQ
GGIGDIYNFKLAPSLTLGCGSWGGNSVSENVGPKHLLNIKNVAERRENMLWFRVPEKVYFKYGCLPIALKELKRMNKKKA
FIVTDKVLYELGVAKKATDVLDEIGINYKVFFDVAPDPTLETAKKGAKEMVDFNPDTIIAIGGGSAMDAAKIMWVMYEHP
EAEFEDLAMRFMDIRKRVYEFPHMGDKAMMISVATSAGTGSEVTPFAVITDEKTGVKYPLADYELTPDMAIVDADLMLNM
PKGLTAASGIDALTHAVEAYVSVMASEYTDGLCLEAIKTIFEYLPKAYKEGAQDIEAREKMAHASTIAGMAFANAFLGVC
HSMAHKLGSMHHVPHGIANALLINETIKFNSEDMPRKQTAFPQYKYPNAKAKYANIADYLSLGGKTPEEKVELLIKAIDK
LKAEVNIPTSIEEAGISKDKFFKTLDEMSEQAFDDQCTGANPRYPLISEIKQMYTNVFSTKK

Sequences:

>Translated_862_residues
MKITTTEELIRKIEKIKEAQKIYSTYSQDKVDKIFKAAAIAANKERIKLAKMAVEETGMGIVEDKVIKNHFASEYIYNKY
KDEKTCGVIEKDEAFGLTKIAEPIGVIAAIVPTTNPTSTAIFKALIALKTRNGIIFSPHPRAKKSTIMAAKIVLDAAVQA
GAPKEIIGWIDEPTLELSNAVMSNSNLVLATGGPGMVKAAYSSGKPAIGVGPGNVPAIIYETADIKMAVSSVVLSKTFDN
GMICASEQSVIVMNSIYEEVKKEFVIRGAYVLNKEEIEKVKKIILVNGNVNAKIVGQTPQKIGEMAGVKVPDWAKLLVGE
VQSVELEEPFSHEKLSPVLAMYKVKTYEEALTKAERLVELGGFGHTSSLYINTVKCKEEVEKFSNNMKTGRTIINMPSAQ
GGIGDIYNFKLAPSLTLGCGSWGGNSVSENVGPKHLLNIKNVAERRENMLWFRVPEKVYFKYGCLPIALKELKRMNKKKA
FIVTDKVLYELGVAKKATDVLDEIGINYKVFFDVAPDPTLETAKKGAKEMVDFNPDTIIAIGGGSAMDAAKIMWVMYEHP
EAEFEDLAMRFMDIRKRVYEFPHMGDKAMMISVATSAGTGSEVTPFAVITDEKTGVKYPLADYELTPDMAIVDADLMLNM
PKGLTAASGIDALTHAVEAYVSVMASEYTDGLCLEAIKTIFEYLPKAYKEGAQDIEAREKMAHASTIAGMAFANAFLGVC
HSMAHKLGSMHHVPHGIANALLINETIKFNSEDMPRKQTAFPQYKYPNAKAKYANIADYLSLGGKTPEEKVELLIKAIDK
LKAEVNIPTSIEEAGISKDKFFKTLDEMSEQAFDDQCTGANPRYPLISEIKQMYTNVFSTKK
>Mature_862_residues
MKITTTEELIRKIEKIKEAQKIYSTYSQDKVDKIFKAAAIAANKERIKLAKMAVEETGMGIVEDKVIKNHFASEYIYNKY
KDEKTCGVIEKDEAFGLTKIAEPIGVIAAIVPTTNPTSTAIFKALIALKTRNGIIFSPHPRAKKSTIMAAKIVLDAAVQA
GAPKEIIGWIDEPTLELSNAVMSNSNLVLATGGPGMVKAAYSSGKPAIGVGPGNVPAIIYETADIKMAVSSVVLSKTFDN
GMICASEQSVIVMNSIYEEVKKEFVIRGAYVLNKEEIEKVKKIILVNGNVNAKIVGQTPQKIGEMAGVKVPDWAKLLVGE
VQSVELEEPFSHEKLSPVLAMYKVKTYEEALTKAERLVELGGFGHTSSLYINTVKCKEEVEKFSNNMKTGRTIINMPSAQ
GGIGDIYNFKLAPSLTLGCGSWGGNSVSENVGPKHLLNIKNVAERRENMLWFRVPEKVYFKYGCLPIALKELKRMNKKKA
FIVTDKVLYELGVAKKATDVLDEIGINYKVFFDVAPDPTLETAKKGAKEMVDFNPDTIIAIGGGSAMDAAKIMWVMYEHP
EAEFEDLAMRFMDIRKRVYEFPHMGDKAMMISVATSAGTGSEVTPFAVITDEKTGVKYPLADYELTPDMAIVDADLMLNM
PKGLTAASGIDALTHAVEAYVSVMASEYTDGLCLEAIKTIFEYLPKAYKEGAQDIEAREKMAHASTIAGMAFANAFLGVC
HSMAHKLGSMHHVPHGIANALLINETIKFNSEDMPRKQTAFPQYKYPNAKAKYANIADYLSLGGKTPEEKVELLIKAIDK
LKAEVNIPTSIEEAGISKDKFFKTLDEMSEQAFDDQCTGANPRYPLISEIKQMYTNVFSTKK

Specific function: This enzyme has probably two activities:ADH, and ACDH [H]

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=327, Percent_Identity=28.4403669724771, Blast_Score=100, Evalue=1e-20,
Organism=Escherichia coli, GI1787493, Length=862, Percent_Identity=62.4129930394432, Blast_Score=1109, Evalue=0.0,
Organism=Escherichia coli, GI1789163, Length=405, Percent_Identity=33.8271604938272, Blast_Score=229, Evalue=5e-61,
Organism=Escherichia coli, GI48994951, Length=406, Percent_Identity=35.4679802955665, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI87082107, Length=403, Percent_Identity=31.5136476426799, Blast_Score=200, Evalue=3e-52,
Organism=Escherichia coli, GI1788797, Length=375, Percent_Identity=32.2666666666667, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789386, Length=405, Percent_Identity=23.9506172839506, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17537053, Length=328, Percent_Identity=25.9146341463415, Blast_Score=108, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6321181, Length=401, Percent_Identity=33.6658354114713, Blast_Score=216, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323821, Length=371, Percent_Identity=23.1805929919137, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24657991, Length=324, Percent_Identity=26.8518518518519, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 94649; Mature: 94649

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITTTEELIRKIEKIKEAQKIYSTYSQDKVDKIFKAAAIAANKERIKLAKMAVEETGMG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
IVEDKVIKNHFASEYIYNKYKDEKTCGVIEKDEAFGLTKIAEPIGVIAAIVPTTNPTSTA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHEEEECCCCCHHHH
IFKALIALKTRNGIIFSPHPRAKKSTIMAAKIVLDAAVQAGAPKEIIGWIDEPTLELSNA
HHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHH
VMSNSNLVLATGGPGMVKAAYSSGKPAIGVGPGNVPAIIYETADIKMAVSSVVLSKTFDN
HCCCCCEEEEECCCCCEEEECCCCCCEEEECCCCCCEEEEECCHHHHHHHHHHHHHHCCC
GMICASEQSVIVMNSIYEEVKKEFVIRGAYVLNKEEIEKVKKIILVNGNVNAKIVGQTPQ
CEEEECCCCEEEHHHHHHHHHHHHHHHHHEEECHHHHHHHHEEEEECCCCCEEEECCCHH
KIGEMAGVKVPDWAKLLVGEVQSVELEEPFSHEKLSPVLAMYKVKTYEEALTKAERLVEL
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGFGHTSSLYINTVKCKEEVEKFSNNMKTGRTIINMPSAQGGIGDIYNFKLAPSLTLGCG
CCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEECC
SWGGNSVSENVGPKHLLNIKNVAERRENMLWFRVPEKVYFKYGCLPIALKELKRMNKKKA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCHHHHHHHCCHHHHHHHHHHHCCCCE
FIVTDKVLYELGVAKKATDVLDEIGINYKVFFDVAPDPTLETAKKGAKEMVDFNPDTIIA
EEEEHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEE
IGGGSAMDAAKIMWVMYEHPEAEFEDLAMRFMDIRKRVYEFPHMGDKAMMISVATSAGTG
ECCCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC
SEVTPFAVITDEKTGVKYPLADYELTPDMAIVDADLMLNMPKGLTAASGIDALTHAVEAY
CCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHEECCCCCCHHHHHHHHHHHHHHH
VSVMASEYTDGLCLEAIKTIFEYLPKAYKEGAQDIEAREKMAHASTIAGMAFANAFLGVC
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSMAHKLGSMHHVPHGIANALLINETIKFNSEDMPRKQTAFPQYKYPNAKAKYANIADYL
HHHHHHHCCCCCCCHHHHHHHHEECCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHH
SLGGKTPEEKVELLIKAIDKLKAEVNIPTSIEEAGISKDKFFKTLDEMSEQAFDDQCTGA
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
NPRYPLISEIKQMYTNVFSTKK
CCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKITTTEELIRKIEKIKEAQKIYSTYSQDKVDKIFKAAAIAANKERIKLAKMAVEETGMG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
IVEDKVIKNHFASEYIYNKYKDEKTCGVIEKDEAFGLTKIAEPIGVIAAIVPTTNPTSTA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHEEEECCCCCHHHH
IFKALIALKTRNGIIFSPHPRAKKSTIMAAKIVLDAAVQAGAPKEIIGWIDEPTLELSNA
HHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHH
VMSNSNLVLATGGPGMVKAAYSSGKPAIGVGPGNVPAIIYETADIKMAVSSVVLSKTFDN
HCCCCCEEEEECCCCCEEEECCCCCCEEEECCCCCCEEEEECCHHHHHHHHHHHHHHCCC
GMICASEQSVIVMNSIYEEVKKEFVIRGAYVLNKEEIEKVKKIILVNGNVNAKIVGQTPQ
CEEEECCCCEEEHHHHHHHHHHHHHHHHHEEECHHHHHHHHEEEEECCCCCEEEECCCHH
KIGEMAGVKVPDWAKLLVGEVQSVELEEPFSHEKLSPVLAMYKVKTYEEALTKAERLVEL
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGFGHTSSLYINTVKCKEEVEKFSNNMKTGRTIINMPSAQGGIGDIYNFKLAPSLTLGCG
CCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEECC
SWGGNSVSENVGPKHLLNIKNVAERRENMLWFRVPEKVYFKYGCLPIALKELKRMNKKKA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCHHHHHHHCCHHHHHHHHHHHCCCCE
FIVTDKVLYELGVAKKATDVLDEIGINYKVFFDVAPDPTLETAKKGAKEMVDFNPDTIIA
EEEEHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEE
IGGGSAMDAAKIMWVMYEHPEAEFEDLAMRFMDIRKRVYEFPHMGDKAMMISVATSAGTG
ECCCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC
SEVTPFAVITDEKTGVKYPLADYELTPDMAIVDADLMLNMPKGLTAASGIDALTHAVEAY
CCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHEECCCCCCHHHHHHHHHHHHHHH
VSVMASEYTDGLCLEAIKTIFEYLPKAYKEGAQDIEAREKMAHASTIAGMAFANAFLGVC
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSMAHKLGSMHHVPHGIANALLINETIKFNSEDMPRKQTAFPQYKYPNAKAKYANIADYL
HHHHHHHCCCCCCCHHHHHHHHEECCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHH
SLGGKTPEEKVELLIKAIDKLKAEVNIPTSIEEAGISKDKFFKTLDEMSEQAFDDQCTGA
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
NPRYPLISEIKQMYTNVFSTKK
CCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226639; 8300540; 11466286 [H]