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The map label for this gene is lgrE [H]

Identifier: 226947530

GI number: 226947530

Start: 354759

End: 355475

Strand: Direct

Name: lgrE [H]

Synonym: CLM_0366

Alternate gene names: 226947530

Gene position: 354759-355475 (Clockwise)

Preceding gene: 226947529

Following gene: 226947531

Centisome position: 8.54

GC content: 25.66

Gene sequence:

>717_bases
ATGATATTATTTTGTTTACCATATGCAGGAGGATCAGAAGCCATTTATTATAAGTGGAAAAAGTATTTAAATCCTTCTAT
AGAGTTAAGTCCAATTGAACTTAAGGGGAGAGGTAAAAGGTATAATGAGATTTTTTATGAAACCATAGATGAGGCAGTGG
AAGATATACTTAAAAATATTAAAGATAGAATAATAGATAATGATTATGCTATTTATGGTCATAGCATGGGAAGTCTTTTA
GCTTATGAATTATATTATAAAATAAAGAAACAAAAGCTTAGAAAACCTATACACATATTTTTCTCAGGTTACAAAGCGCC
AAATATTATAAGAAAAAAAGAGAATATATATACTTTACCAGATTATGACTTTATTAAAAAGGTGATTGAGCTTGGAGGTA
CACCAGAAGAGCTATCTAATAATAAAGAATTACTTGACATATTCATTCCTATAATTCGAAATGATTTTAAAATTTTAGAA
AATTATAATTATGAAAAAAAAGAAGATAAGATTGAGTGTGATGTTTCCATTTTAAATGGAAAACAGGATTCTATAAATTT
AGAAGAAATATTAGCTTGGAAAAAGCATGTGGGAAAAGGCTTTAAAGTGTACAGTTTTGAAGGAGATCATTTTTTTATAA
ATAATAATGTTAAAAATATAAATAGTATTATAAACAGTACCCTGATTGAATATATAGATAATCAATATGCAAATTGA

Upstream 100 bases:

>100_bases
TAAGTAAGAGGATTTCAAGGAATACTATAATGATAGATCAAAATAGTTTGATTAACAGATATTTAGATTTAATATAAATT
AAGTAAGGGGAGTTACACCA

Downstream 100 bases:

>100_bases
AATAGAAATTATTTCAGTTTATATAATACTTGATATTTTAGTATAATTAAGAATTTTGATGATAGCTATAGGCTTTGGTG
GATATTAAAAAACTTTATAT

Product: linear gramicidin dehydrogenase LgrE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MILFCLPYAGGSEAIYYKWKKYLNPSIELSPIELKGRGKRYNEIFYETIDEAVEDILKNIKDRIIDNDYAIYGHSMGSLL
AYELYYKIKKQKLRKPIHIFFSGYKAPNIIRKKENIYTLPDYDFIKKVIELGGTPEELSNNKELLDIFIPIIRNDFKILE
NYNYEKKEDKIECDVSILNGKQDSINLEEILAWKKHVGKGFKVYSFEGDHFFINNNVKNINSIINSTLIEYIDNQYAN

Sequences:

>Translated_238_residues
MILFCLPYAGGSEAIYYKWKKYLNPSIELSPIELKGRGKRYNEIFYETIDEAVEDILKNIKDRIIDNDYAIYGHSMGSLL
AYELYYKIKKQKLRKPIHIFFSGYKAPNIIRKKENIYTLPDYDFIKKVIELGGTPEELSNNKELLDIFIPIIRNDFKILE
NYNYEKKEDKIECDVSILNGKQDSINLEEILAWKKHVGKGFKVYSFEGDHFFINNNVKNINSIINSTLIEYIDNQYAN
>Mature_238_residues
MILFCLPYAGGSEAIYYKWKKYLNPSIELSPIELKGRGKRYNEIFYETIDEAVEDILKNIKDRIIDNDYAIYGHSMGSLL
AYELYYKIKKQKLRKPIHIFFSGYKAPNIIRKKENIYTLPDYDFIKKVIELGGTPEELSNNKELLDIFIPIIRNDFKILE
NYNYEKKEDKIECDVSILNGKQDSINLEEILAWKKHVGKGFKVYSFEGDHFFINNNVKNINSIINSTLIEYIDNQYAN

Specific function: In the final step of gramicidin biosynthesis, reduces the pentadecapeptide-aldehyde intermediate, that is released from the terminal module of the non-ribosomal peptide synthetase LgrD, to the final product ethanolamine-containing gramicidin [H]

COG id: COG3208

COG function: function code Q; Predicted thioesterase involved in non-ribosomal peptide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase family [H]

Homologues:

Organism=Homo sapiens, GI89257335, Length=217, Percent_Identity=27.6497695852535, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI8922871, Length=188, Percent_Identity=27.6595744680851, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012223
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00975 Thioesterase [H]

EC number: NA

Molecular weight: Translated: 27998; Mature: 27998

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILFCLPYAGGSEAIYYKWKKYLNPSIELSPIELKGRGKRYNEIFYETIDEAVEDILKNI
CEEEEEECCCCCCEEEEEEHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KDRIIDNDYAIYGHSMGSLLAYELYYKIKKQKLRKPIHIFFSGYKAPNIIRKKENIYTLP
HHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEECCCCEEECC
DYDFIKKVIELGGTPEELSNNKELLDIFIPIIRNDFKILENYNYEKKEDKIECDVSILNG
CHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCHHHHHHCCCCCCCCCCEEEEEEEECC
KQDSINLEEILAWKKHVGKGFKVYSFEGDHFFINNNVKNINSIINSTLIEYIDNQYAN
CCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILFCLPYAGGSEAIYYKWKKYLNPSIELSPIELKGRGKRYNEIFYETIDEAVEDILKNI
CEEEEEECCCCCCEEEEEEHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KDRIIDNDYAIYGHSMGSLLAYELYYKIKKQKLRKPIHIFFSGYKAPNIIRKKENIYTLP
HHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEECCCCEEECC
DYDFIKKVIELGGTPEELSNNKELLDIFIPIIRNDFKILENYNYEKKEDKIECDVSILNG
CHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCHHHHHHCCCCCCCCCCEEEEEEEECC
KQDSINLEEILAWKKHVGKGFKVYSFEGDHFFINNNVKNINSIINSTLIEYIDNQYAN
CCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA