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The map label for this gene is sfp [H]

Identifier: 226947529

GI number: 226947529

Start: 354013

End: 354735

Strand: Direct

Name: sfp [H]

Synonym: CLM_0365

Alternate gene names: 226947529

Gene position: 354013-354735 (Clockwise)

Preceding gene: 226947528

Following gene: 226947530

Centisome position: 8.52

GC content: 22.82

Gene sequence:

>723_bases
ATGAAGAAAGATGTAAATGAGGAATTTATGAAGATTTATGCTGTAAAAAATTTGGATATAAATAAAGAAAAGTTATGTGA
GTTATCTTTACTTATAGACAAAGAGAAAAGATATAAAATTGAAAAATTTATTAATAAAGAAGATAAAATTAGAACATTAA
TCGGTGAAATATTGGTAAGAGTAATAATCAATGAAAACTTAGGCATTACAAATAATCATATAACCTTTGAGAAAAACAAA
TATGGGAAGCCATGTTTGAAAAATTATGAAAATTTTAATTTTAACATATCTCATTCTGGAGATTTTGTAGCATGTGTAAT
TGATGATAAGCCAGTAGGAATTGATATTGAAAAAATAAAACATATTGAGTATGAAGATATAGCTAAAAGTTTTTTTACTA
TAAATGAATACGAATACATTATTAAAAATGATCCATATACTCCATTGAGTAAATTTTATAAAATATGGACTTTGAAAGAA
AGCTATATTAAATGTTGTGGACAAGGTCTATCAATACCCTTAAAATCATTTTCTATTGATATAGATAAAAATAAAAGTAT
TAAAATGCTTAATGATAATAATCATAATGGGTATATATTTAAGAGCTTTGATATAGACTTACATTATAAAATGGCTGTAT
GCTCATTAAGTAAGAGGATTTCAAGGAATACTATAATGATAGATCAAAATAGTTTGATTAACAGATATTTAGATTTAATA
TAA

Upstream 100 bases:

>100_bases
TTTAAAGATATGTATAATGTAATTGAGATAAATTACTTAATAAGTATTATATATCTTATATTATATGTAAGTGGAAAAGA
GTATTAGTTTTAAATAAATT

Downstream 100 bases:

>100_bases
ATTAAGTAAGGGGAGTTACACCAATGATATTATTTTGTTTACCATATGCAGGAGGATCAGAAGCCATTTATTATAAGTGG
AAAAAGTATTTAAATCCTTC

Product: 4'-phosphopantetheinyl transferase sfp

Products: adenosine 3',5'-bisphosphate [C]

Alternate protein names: Surfactin synthase-activating enzyme [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKKDVNEEFMKIYAVKNLDINKEKLCELSLLIDKEKRYKIEKFINKEDKIRTLIGEILVRVIINENLGITNNHITFEKNK
YGKPCLKNYENFNFNISHSGDFVACVIDDKPVGIDIEKIKHIEYEDIAKSFFTINEYEYIIKNDPYTPLSKFYKIWTLKE
SYIKCCGQGLSIPLKSFSIDIDKNKSIKMLNDNNHNGYIFKSFDIDLHYKMAVCSLSKRISRNTIMIDQNSLINRYLDLI

Sequences:

>Translated_240_residues
MKKDVNEEFMKIYAVKNLDINKEKLCELSLLIDKEKRYKIEKFINKEDKIRTLIGEILVRVIINENLGITNNHITFEKNK
YGKPCLKNYENFNFNISHSGDFVACVIDDKPVGIDIEKIKHIEYEDIAKSFFTINEYEYIIKNDPYTPLSKFYKIWTLKE
SYIKCCGQGLSIPLKSFSIDIDKNKSIKMLNDNNHNGYIFKSFDIDLHYKMAVCSLSKRISRNTIMIDQNSLINRYLDLI
>Mature_240_residues
MKKDVNEEFMKIYAVKNLDINKEKLCELSLLIDKEKRYKIEKFINKEDKIRTLIGEILVRVIINENLGITNNHITFEKNK
YGKPCLKNYENFNFNISHSGDFVACVIDDKPVGIDIEKIKHIEYEDIAKSFFTINEYEYIIKNDPYTPLSKFYKIWTLKE
SYIKCCGQGLSIPLKSFSIDIDKNKSIKMLNDNNHNGYIFKSFDIDLHYKMAVCSLSKRISRNTIMIDQNSLINRYLDLI

Specific function: Activates the seven peptidyl carrier protein (PCP) domains of surfactin synthase SRF1/2/3 by transferring the 4'- phosphopantetheinyl moiety of coenzyme A (CoA) to a serine residue. Required for cells of B.subtilis to become producers of the lipopeptide a

COG id: COG2091

COG function: function code H; Phosphopantetheinyl transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. Gsp/sfp/hetI/acpT family [H]

Homologues:

Organism=Homo sapiens, GI20357568, Length=163, Percent_Identity=36.1963190184049, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17564880, Length=174, Percent_Identity=27.5862068965517, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17569611, Length=217, Percent_Identity=27.1889400921659, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24663173, Length=185, Percent_Identity=26.4864864864865, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR004568 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: NA

Molecular weight: Translated: 28267; Mature: 28267

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDVNEEFMKIYAVKNLDINKEKLCELSLLIDKEKRYKIEKFINKEDKIRTLIGEILVR
CCCCHHHHHHEEEEEECCCCCHHHHHEEHEEECCHHHHHHHHHCCCHHHHHHHHHHHHHH
VIINENLGITNNHITFEKNKYGKPCLKNYENFNFNISHSGDFVACVIDDKPVGIDIEKIK
HHHCCCCCCCCCEEEEECCCCCCHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHH
HIEYEDIAKSFFTINEYEYIIKNDPYTPLSKFYKIWTLKESYIKCCGQGLSIPLKSFSID
CCCHHHHHHHHEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEHEEEE
IDKNKSIKMLNDNNHNGYIFKSFDIDLHYKMAVCSLSKRISRNTIMIDQNSLINRYLDLI
ECCCCCEEEEECCCCCCEEEEEECCEEEHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCC
>Mature Secondary Structure
MKKDVNEEFMKIYAVKNLDINKEKLCELSLLIDKEKRYKIEKFINKEDKIRTLIGEILVR
CCCCHHHHHHEEEEEECCCCCHHHHHEEHEEECCHHHHHHHHHCCCHHHHHHHHHHHHHH
VIINENLGITNNHITFEKNKYGKPCLKNYENFNFNISHSGDFVACVIDDKPVGIDIEKIK
HHHCCCCCCCCCEEEEECCCCCCHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHH
HIEYEDIAKSFFTINEYEYIIKNDPYTPLSKFYKIWTLKESYIKCCGQGLSIPLKSFSID
CCCHHHHHHHHEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEHEEEE
IDKNKSIKMLNDNNHNGYIFKSFDIDLHYKMAVCSLSKRISRNTIMIDQNSLINRYLDLI
ECCCCCEEEEECCCCCCEEEEEECCEEEHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; apo-[acyl carrier protein] [C]

Specific reaction: Coenzyme A + apo-[acyl carrier protein] = adenosine 3',5'-bisphosphate + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1557038; 8407792; 8355609; 8639027; 9384377; 8939709; 9484229; 10581256 [H]