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The map label for this gene is cssS [H]

Identifier: 226947520

GI number: 226947520

Start: 341853

End: 343241

Strand: Direct

Name: cssS [H]

Synonym: CLM_0354

Alternate gene names: 226947520

Gene position: 341853-343241 (Clockwise)

Preceding gene: 226947519

Following gene: 226947521

Centisome position: 8.23

GC content: 25.85

Gene sequence:

>1389_bases
ATGATGAGGAGATTAAGATTTAAATCGTTAACAATGAGGATATGGACAACATTCACTGCTATAATTTTAATAATAATATG
TAGTATTTCATTTTTATATTTAGTTGCCTTTAAGAGAATTAGTGAAAATTCAAAGCTAGAGGATTTAAAAGTCGCTCATG
ATGTTTTATTAAACAGCAATAATTTTAATGAACAAAATAGATTTGATGAATTAAGAAATCTTAAGGGAAGTGATCATTTT
ATTGTAAAAATAGATGAGAATGATAAATATAAAATTATAGATATAGGTAAAAGAAAAGAGGAGCCACCAGCAATAGAAAA
GAAGAATCCACCCCCAAGACCTACTAGTGATGAAATAAAAATGTGGATGGCTAGTTATATTATAGGTAATAATATTCAGC
AAAAGCAATTTAAAGAATATCATAACAACATGAAATTTATCTTTATAATAAGTTCAATTAAAAATGCAGGAGGGGAAAAA
CTTTATTTAATATCGTATATACCTGAAATACAAGATACTGTATTGTTATATACAGTAATAATTATAGGCATTATATTTAT
TGTTATAGGATTTTTTACTGCTAAATTAGTTGCAAATTATATTTCAAGGCCTTTAAGAGAGCTTGAGGATTATACTGTAA
AAATAGCTCATAAGGATTGGAGGGAGCCTGTTAAAATTAAAAATGATGATGAGATAGGTAGATTGGTAGATTCAATGAAT
CGTATGCAAAAAGAATTAAAAAAGATTGATGAAGAAGAAAAAATGTTTCTACAAAGCATATCTCATGATTTAAAGACTCC
AGTAATGGTAATTATGAGCCATGCTCAAGCTATTATTGATGGTATGTATATAGAATCAGTTGAAGAAACTGCTGAGATAA
TAAAAGATGAGGCAGCTATTCTTGAGAAAAAAATTAAACAATTATTGTATTTGAATACTCTTGCCTATACTTTAGAAAAT
GATAGTGAAAATATAGGATTTGAGTTACATAATTTATTATTTAATATTATAAGTAGATTTGAGATAGTTAATAGTAAAAT
TCAATGGAATTTAGATATTGATAAGGTTGTTATAAAAGGAAATGCTGAAAAAATACAAGTAGCTATTGAAAATATATTAG
ATAATGGACTTAGGTATGCAGAAAGGGAAATTGCCATAACTCTTAAAAGAGAAAATTCTGTTGCAGTCCTAGAAATTTAT
AATGATGGACCTAATATTGATAAAAATAATATAGAGCATATTTTTGAAAATTTATATAAAGATAGAACTGGAAATTTTGG
ACTAGGTTTAGCTATTTCTAAAAAAATTATAGATTTTTATAATGGAGAAATAAAGGCAGTAAATAGAGATAATGGGGTAA
GTTTTATAATTAAATATCCTATAGATTAA

Upstream 100 bases:

>100_bases
CCGATAGAGTTATTGATGATACAATTAGGAGATTGCGCAAAAAGATGGATAAGTTAGATCTTGAAACTGTATATGGATAT
GGTTATAAATTGGTGGTTAA

Downstream 100 bases:

>100_bases
TAATTGGCCTTCGAAAAAGTAAAAGTAATTATTATTTACACTTCCCAATAAATTTTAATAATTAAGTTTAGAGGGTGTCT
CAAAATAAAAATAGCCTTAA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MMRRLRFKSLTMRIWTTFTAIILIIICSISFLYLVAFKRISENSKLEDLKVAHDVLLNSNNFNEQNRFDELRNLKGSDHF
IVKIDENDKYKIIDIGKRKEEPPAIEKKNPPPRPTSDEIKMWMASYIIGNNIQQKQFKEYHNNMKFIFIISSIKNAGGEK
LYLISYIPEIQDTVLLYTVIIIGIIFIVIGFFTAKLVANYISRPLRELEDYTVKIAHKDWREPVKIKNDDEIGRLVDSMN
RMQKELKKIDEEEKMFLQSISHDLKTPVMVIMSHAQAIIDGMYIESVEETAEIIKDEAAILEKKIKQLLYLNTLAYTLEN
DSENIGFELHNLLFNIISRFEIVNSKIQWNLDIDKVVIKGNAEKIQVAIENILDNGLRYAEREIAITLKRENSVAVLEIY
NDGPNIDKNNIEHIFENLYKDRTGNFGLGLAISKKIIDFYNGEIKAVNRDNGVSFIIKYPID

Sequences:

>Translated_462_residues
MMRRLRFKSLTMRIWTTFTAIILIIICSISFLYLVAFKRISENSKLEDLKVAHDVLLNSNNFNEQNRFDELRNLKGSDHF
IVKIDENDKYKIIDIGKRKEEPPAIEKKNPPPRPTSDEIKMWMASYIIGNNIQQKQFKEYHNNMKFIFIISSIKNAGGEK
LYLISYIPEIQDTVLLYTVIIIGIIFIVIGFFTAKLVANYISRPLRELEDYTVKIAHKDWREPVKIKNDDEIGRLVDSMN
RMQKELKKIDEEEKMFLQSISHDLKTPVMVIMSHAQAIIDGMYIESVEETAEIIKDEAAILEKKIKQLLYLNTLAYTLEN
DSENIGFELHNLLFNIISRFEIVNSKIQWNLDIDKVVIKGNAEKIQVAIENILDNGLRYAEREIAITLKRENSVAVLEIY
NDGPNIDKNNIEHIFENLYKDRTGNFGLGLAISKKIIDFYNGEIKAVNRDNGVSFIIKYPID
>Mature_462_residues
MMRRLRFKSLTMRIWTTFTAIILIIICSISFLYLVAFKRISENSKLEDLKVAHDVLLNSNNFNEQNRFDELRNLKGSDHF
IVKIDENDKYKIIDIGKRKEEPPAIEKKNPPPRPTSDEIKMWMASYIIGNNIQQKQFKEYHNNMKFIFIISSIKNAGGEK
LYLISYIPEIQDTVLLYTVIIIGIIFIVIGFFTAKLVANYISRPLRELEDYTVKIAHKDWREPVKIKNDDEIGRLVDSMN
RMQKELKKIDEEEKMFLQSISHDLKTPVMVIMSHAQAIIDGMYIESVEETAEIIKDEAAILEKKIKQLLYLNTLAYTLEN
DSENIGFELHNLLFNIISRFEIVNSKIQWNLDIDKVVIKGNAEKIQVAIENILDNGLRYAEREIAITLKRENSVAVLEIY
NDGPNIDKNNIEHIFENLYKDRTGNFGLGLAISKKIIDFYNGEIKAVNRDNGVSFIIKYPID

Specific function: Member of the two-component regulatory system CssS/CssR required to control the cellular response to secretion stress. Required for the transcription of htrA. Could detect misfolded proteins at the membrane-cell wall interface and then activate CssR by ph

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=273, Percent_Identity=27.1062271062271, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789808, Length=290, Percent_Identity=24.4827586206897, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790551, Length=289, Percent_Identity=21.4532871972318, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790346, Length=269, Percent_Identity=26.0223048327138, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87081816, Length=335, Percent_Identity=20.8955223880597, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=22.5108225108225, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53692; Mature: 53692

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRRLRFKSLTMRIWTTFTAIILIIICSISFLYLVAFKRISENSKLEDLKVAHDVLLNSN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
NFNEQNRFDELRNLKGSDHFIVKIDENDKYKIIDIGKRKEEPPAIEKKNPPPRPTSDEIK
CCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
MWMASYIIGNNIQQKQFKEYHNNMKFIFIISSIKNAGGEKLYLISYIPEIQDTVLLYTVI
HHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHH
IIGIIFIVIGFFTAKLVANYISRPLRELEDYTVKIAHKDWREPVKIKNDDEIGRLVDSMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHH
RMQKELKKIDEEEKMFLQSISHDLKTPVMVIMSHAQAIIDGMYIESVEETAEIIKDEAAI
HHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKKIKQLLYLNTLAYTLENDSENIGFELHNLLFNIISRFEIVNSKIQWNLDIDKVVIKG
HHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEC
NAEKIQVAIENILDNGLRYAEREIAITLKRENSVAVLEIYNDGPNIDKNNIEHIFENLYK
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHH
DRTGNFGLGLAISKKIIDFYNGEIKAVNRDNGVSFIIKYPID
CCCCCCCEEHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCC
>Mature Secondary Structure
MMRRLRFKSLTMRIWTTFTAIILIIICSISFLYLVAFKRISENSKLEDLKVAHDVLLNSN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
NFNEQNRFDELRNLKGSDHFIVKIDENDKYKIIDIGKRKEEPPAIEKKNPPPRPTSDEIK
CCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
MWMASYIIGNNIQQKQFKEYHNNMKFIFIISSIKNAGGEKLYLISYIPEIQDTVLLYTVI
HHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHH
IIGIIFIVIGFFTAKLVANYISRPLRELEDYTVKIAHKDWREPVKIKNDDEIGRLVDSMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHH
RMQKELKKIDEEEKMFLQSISHDLKTPVMVIMSHAQAIIDGMYIESVEETAEIIKDEAAI
HHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKKIKQLLYLNTLAYTLENDSENIGFELHNLLFNIISRFEIVNSKIQWNLDIDKVVIKG
HHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEC
NAEKIQVAIENILDNGLRYAEREIAITLKRENSVAVLEIYNDGPNIDKNNIEHIFENLYK
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHH
DRTGNFGLGLAISKKIIDFYNGEIKAVNRDNGVSFIIKYPID
CCCCCCCEEHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353931; 9639930; 9384377; 11555295 [H]