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The map label for this gene is smpB

Identifier: 226947455

GI number: 226947455

Start: 272721

End: 273191

Strand: Direct

Name: smpB

Synonym: CLM_0284

Alternate gene names: 226947455

Gene position: 272721-273191 (Clockwise)

Preceding gene: 226947454

Following gene: 226947458

Centisome position: 6.56

GC content: 31.0

Gene sequence:

>471_bases
ATGAGTAAAAAGAAGGGAAGCAATACTCTTGCAGAAAATAGAAAAGCAAGGCATGATTATTTTATAGAAGAAACTTACGA
AGCTGGTATAGAATTAGTAGGGACAGAGGTTAAATCTATAAGACAAGGAAAAGCAAACTTAAAAGATAGTTATGCTGAGA
TTAGAAATGGGGAAGTTTTTGTTAGAAACATGCATATAAGTCCCTATGAACAAGGAAACATATATAATAAAGATCCACTA
AGAGATAGAAAATTGTTACTTCATAAGAGTGAAATATATAAATTAGTAGGCTTTACAACTCAACAGGGGTATACCCTGAT
TCCTTTATCATTATATCTTAAACATGGAAGAGTAAAAGTTAGTTTAGCTGTAGCTAAGGGTAAGAAAAATTATGATAAGA
GAGATGCAATGCTAGAAAAGGCAGCAAAAAGAGAAATGGACAGACAAATTAAAGAAAGAAGTAGATATTAA

Upstream 100 bases:

>100_bases
ATTCAAGTTCATTACAATAGTATGTTTGAAATGAATTTACATAGATGATATACTATGTATTATTATAACTAAAAAAGTTA
TTCCGTTAGGCGGTGATTTC

Downstream 100 bases:

>100_bases
AAGTAAATGAAGGTGTATTAAAATATATTTAATTTTAATACTGCTACGATAAAAAATACATTTCATAAACTAATTTGTTT
ATGAAATGTATTTTTTTATC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MSKKKGSNTLAENRKARHDYFIEETYEAGIELVGTEVKSIRQGKANLKDSYAEIRNGEVFVRNMHISPYEQGNIYNKDPL
RDRKLLLHKSEIYKLVGFTTQQGYTLIPLSLYLKHGRVKVSLAVAKGKKNYDKRDAMLEKAAKREMDRQIKERSRY

Sequences:

>Translated_156_residues
MSKKKGSNTLAENRKARHDYFIEETYEAGIELVGTEVKSIRQGKANLKDSYAEIRNGEVFVRNMHISPYEQGNIYNKDPL
RDRKLLLHKSEIYKLVGFTTQQGYTLIPLSLYLKHGRVKVSLAVAKGKKNYDKRDAMLEKAAKREMDRQIKERSRY
>Mature_155_residues
SKKKGSNTLAENRKARHDYFIEETYEAGIELVGTEVKSIRQGKANLKDSYAEIRNGEVFVRNMHISPYEQGNIYNKDPLR
DRKLLLHKSEIYKLVGFTTQQGYTLIPLSLYLKHGRVKVSLAVAKGKKNYDKRDAMLEKAAKREMDRQIKERSRY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=160, Percent_Identity=46.25, Blast_Score=137, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_CLOB1 (A7FQP4)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382638.1
- ProteinModelPortal:   A7FQP4
- SMR:   A7FQP4
- STRING:   A7FQP4
- GeneID:   5397609
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0275
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   CBOT441770:CLB_0275-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18155; Mature: 18024

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKKGSNTLAENRKARHDYFIEETYEAGIELVGTEVKSIRQGKANLKDSYAEIRNGEVF
CCCCCCCCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEE
VRNMHISPYEQGNIYNKDPLRDRKLLLHKSEIYKLVGFTTQQGYTLIPLSLYLKHGRVKV
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHEEECCEEEE
SLAVAKGKKNYDKRDAMLEKAAKREMDRQIKERSRY
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKKKGSNTLAENRKARHDYFIEETYEAGIELVGTEVKSIRQGKANLKDSYAEIRNGEVF
CCCCCCCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEE
VRNMHISPYEQGNIYNKDPLRDRKLLLHKSEIYKLVGFTTQQGYTLIPLSLYLKHGRVKV
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHEEECCEEEE
SLAVAKGKKNYDKRDAMLEKAAKREMDRQIKERSRY
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA