| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is rpoN [H]
Identifier: 226947445
GI number: 226947445
Start: 259405
End: 260778
Strand: Direct
Name: rpoN [H]
Synonym: CLM_0274
Alternate gene names: 226947445
Gene position: 259405-260778 (Clockwise)
Preceding gene: 226947444
Following gene: 226947446
Centisome position: 6.24
GC content: 26.06
Gene sequence:
>1374_bases ATGAATATGAATTTTGGATTGTCCTTAACACAGGAACAAAAGTTAATAATGACACAACAAATGCAGCTATCTATTAAACT TCTTCAGATGTCTACCTATGAGTTACAACAATATGTTGAAAAGGAAGTTCAAGAAAATCCTGTTTTAGATTCTCAGAATA TTCATAAAGAAGATAAGGGGATAGAGGATATAAATTATAAAAAATTAATCAAACATTTAGAGGATAATGGAAATGAATAT GGTAGTTATAGTAGCTACAATAAAGAAGAAGAAACCTCACCTTTTAATTTTATATCTAATGAAAAATCATTAAAAGAATA CTTACAAGAACAAATTATAGAATTAAATGAAAACAAATACATAAAGTCCATATGCATATACATAATAGAAAATATAGATG ATAAAGGGTATTTATCTGTAAGGGATGAGGAAATATCTGAGGAATTAAAAGTACCATTACATTCAATAGAAGAAGCCATA GATATAGTACAGTCGTTAGAACCAGATGGGATAGGTGCGAGGAATTTAAAAGAATGTTTAAAGATACAATTAAAAAAGAA ATCCATAAATAATAGTAAAATATGTGAAATTATAGACAATTTCCTTCCTCTAGTAGCAGAAAATAGGTATAATGTTATAT CTAAAAAACTTAGTATATCAATAAAAAAGGCTCAGGAATATGGCGATGTTATAAAGACTCTTCAGCCTAAGCCTTCAAGA GGATTTTATACTGGTGATGAAGTAAAATACATAGTTCCAGATGCATATATAAAAAAAATAGATAACGAATACTATATAAT TATGAATGAAGAGCTATTGCCTAAGTTAACAGTAAATAATTTATATAAAGACATAGTTTTAAATGATACTGATGAAAATG CAGTAGAATTTGTAAAAGAAAAATTAAATAGCGCTATCTTTTTAATTAAAAGTATAGAAAATAGAAAATCTACAATTTAT AGAGTATTAGAAAAAATTTTAGAAATACAAAGGGATTATTTTGATTTAGGAATTAAGTTCTTAAAGCCTATGACATTAAA GGAAATAGCCGATAGTTTAGGTATGCATGAGTCTACTATAAGTAGAGCTATAAGAGATAAATATATAAACACTAATAGAG GGACCATACTTATAAAAGATTTATTTACTACAGGAATTACTGCTAATAATAGTACAGGAGAAGATATTTCTGTAGAATTT ATAAAAAGAGAAATAAAAGAACTAATAGATAAAGAAGATAAGAAAAAGCCAATATCTGATCAAATAATATGTAATATATT AAATAATAAAGGCATACAAATATCCAGAAGAACTGTAGCTAAATATAGAGAGGAACTAGGAATACAATCTTCAAAATGTA GAAAGCGTTTTTAA
Upstream 100 bases:
>100_bases CTAATGAATATTAAGGTATATAAGTATTTTTAATTCTTAAGTCTGATGTAGTAAAATTTTTTTTATGATATAATTAAAAT CAAAGGGGGGGTAGGAATTT
Downstream 100 bases:
>100_bases AAGCATGATTTATAGATTATCATGCTTTTTTTATTTAATAATATGAATTAAATTTTTAAAATTAATAAGGGTATAAAATT AAGGATATAGAATTAAAAAT
Product: RNA polymerase factor sigma-54
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 457; Mature: 457
Protein sequence:
>457_residues MNMNFGLSLTQEQKLIMTQQMQLSIKLLQMSTYELQQYVEKEVQENPVLDSQNIHKEDKGIEDINYKKLIKHLEDNGNEY GSYSSYNKEEETSPFNFISNEKSLKEYLQEQIIELNENKYIKSICIYIIENIDDKGYLSVRDEEISEELKVPLHSIEEAI DIVQSLEPDGIGARNLKECLKIQLKKKSINNSKICEIIDNFLPLVAENRYNVISKKLSISIKKAQEYGDVIKTLQPKPSR GFYTGDEVKYIVPDAYIKKIDNEYYIIMNEELLPKLTVNNLYKDIVLNDTDENAVEFVKEKLNSAIFLIKSIENRKSTIY RVLEKILEIQRDYFDLGIKFLKPMTLKEIADSLGMHESTISRAIRDKYINTNRGTILIKDLFTTGITANNSTGEDISVEF IKREIKELIDKEDKKKPISDQIICNILNNKGIQISRRTVAKYREELGIQSSKCRKRF
Sequences:
>Translated_457_residues MNMNFGLSLTQEQKLIMTQQMQLSIKLLQMSTYELQQYVEKEVQENPVLDSQNIHKEDKGIEDINYKKLIKHLEDNGNEY GSYSSYNKEEETSPFNFISNEKSLKEYLQEQIIELNENKYIKSICIYIIENIDDKGYLSVRDEEISEELKVPLHSIEEAI DIVQSLEPDGIGARNLKECLKIQLKKKSINNSKICEIIDNFLPLVAENRYNVISKKLSISIKKAQEYGDVIKTLQPKPSR GFYTGDEVKYIVPDAYIKKIDNEYYIIMNEELLPKLTVNNLYKDIVLNDTDENAVEFVKEKLNSAIFLIKSIENRKSTIY RVLEKILEIQRDYFDLGIKFLKPMTLKEIADSLGMHESTISRAIRDKYINTNRGTILIKDLFTTGITANNSTGEDISVEF IKREIKELIDKEDKKKPISDQIICNILNNKGIQISRRTVAKYREELGIQSSKCRKRF >Mature_457_residues MNMNFGLSLTQEQKLIMTQQMQLSIKLLQMSTYELQQYVEKEVQENPVLDSQNIHKEDKGIEDINYKKLIKHLEDNGNEY GSYSSYNKEEETSPFNFISNEKSLKEYLQEQIIELNENKYIKSICIYIIENIDDKGYLSVRDEEISEELKVPLHSIEEAI DIVQSLEPDGIGARNLKECLKIQLKKKSINNSKICEIIDNFLPLVAENRYNVISKKLSISIKKAQEYGDVIKTLQPKPSR GFYTGDEVKYIVPDAYIKKIDNEYYIIMNEELLPKLTVNNLYKDIVLNDTDENAVEFVKEKLNSAIFLIKSIENRKSTIY RVLEKILEIQRDYFDLGIKFLKPMTLKEIADSLGMHESTISRAIRDKYINTNRGTILIKDLFTTGITANNSTGEDISVEF IKREIKELIDKEDKKKPISDQIICNILNNKGIQISRRTVAKYREELGIQSSKCRKRF
Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]
COG id: COG1508
COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the sigma-54 factor family [H]
Homologues:
Organism=Escherichia coli, GI1789594, Length=481, Percent_Identity=30.5613305613306, Blast_Score=243, Evalue=2e-65,
Paralogues:
None
Copy number: 70 (log & stationary phase) [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000394 - InterPro: IPR007046 - InterPro: IPR007634 [H]
Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]
EC number: NA
Molecular weight: Translated: 53125; Mature: 53125
Theoretical pI: Translated: 5.52; Mature: 5.52
Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNMNFGLSLTQEQKLIMTQQMQLSIKLLQMSTYELQQYVEKEVQENPVLDSQNIHKEDKG CCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC IEDINYKKLIKHLEDNGNEYGSYSSYNKEEETSPFNFISNEKSLKEYLQEQIIELNENKY CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCHH IKSICIYIIENIDDKGYLSVRDEEISEELKVPLHSIEEAIDIVQSLEPDGIGARNLKECL HHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH KIQLKKKSINNSKICEIIDNFLPLVAENRYNVISKKLSISIKKAQEYGDVIKTLQPKPSR HHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC GFYTGDEVKYIVPDAYIKKIDNEYYIIMNEELLPKLTVNNLYKDIVLNDTDENAVEFVKE CCCCCCCCEEECCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH KLNSAIFLIKSIENRKSTIYRVLEKILEIQRDYFDLGIKFLKPMTLKEIADSLGMHESTI HHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH SRAIRDKYINTNRGTILIKDLFTTGITANNSTGEDISVEFIKREIKELIDKEDKKKPISD HHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHH QIICNILNNKGIQISRRTVAKYREELGIQSSKCRKRF HHHHHHHCCCCCEEHHHHHHHHHHHHCCCHHHHHCCC >Mature Secondary Structure MNMNFGLSLTQEQKLIMTQQMQLSIKLLQMSTYELQQYVEKEVQENPVLDSQNIHKEDKG CCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC IEDINYKKLIKHLEDNGNEYGSYSSYNKEEETSPFNFISNEKSLKEYLQEQIIELNENKY CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCHH IKSICIYIIENIDDKGYLSVRDEEISEELKVPLHSIEEAIDIVQSLEPDGIGARNLKECL HHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH KIQLKKKSINNSKICEIIDNFLPLVAENRYNVISKKLSISIKKAQEYGDVIKTLQPKPSR HHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC GFYTGDEVKYIVPDAYIKKIDNEYYIIMNEELLPKLTVNNLYKDIVLNDTDENAVEFVKE CCCCCCCCEEECCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH KLNSAIFLIKSIENRKSTIYRVLEKILEIQRDYFDLGIKFLKPMTLKEIADSLGMHESTI HHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH SRAIRDKYINTNRGTILIKDLFTTGITANNSTGEDISVEFIKREIKELIDKEDKKKPISD HHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHH QIICNILNNKGIQISRRTVAKYREELGIQSSKCRKRF HHHHHHHCCCCCEEHHHHHHHHHHHHCCCHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: DNA [C]
Specific reaction: Protein + DNA = Protein-DNA [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8550525 [H]