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The map label for this gene is prfB [H]

Identifier: 226947396

GI number: 226947396

Start: 204581

End: 205558

Strand: Direct

Name: prfB [H]

Synonym: CLM_0222

Alternate gene names: 226947396

Gene position: 204581-205558 (Clockwise)

Preceding gene: 226947395

Following gene: 226947398

Centisome position: 4.92

GC content: 29.75

Gene sequence:

>978_bases
ATGCAAAACCCAAATTTTTGGAATGATATAAAAGAGGCACAAAAAATAACTTCAGAAGAAAAGTATTTAAAGGATAAGTT
AACCAAATATAATCATTTAAGAAATACTATAGAAGATATTATATCTCTTTGTGAAATGTTATCAGAAGAAGATGATGATA
TGAAAAAAGAAATAATATCAGAATATAAAAATATAAAAGAAGAAACAGATAGATTTAAAATAGAAACCCTATTATCTGGA
GAGTATGATAGAAATAATGTAATAATGACCCTACATTCAGGAGTAGGAGGAACAGACGCGCAGGATTGGACAGATATGCT
ATTTAGGATGTATACAAGGTGGGCAGAAAAAGAAGGATTTACTATTAAGATTATGGATTTATTAGAAGCTGAAGATGCAG
GTATAAAAAGTGTAACCCTAAAGATTACTGGAGAATTTGTATATGGATATCTTAAAGGTGAAAAGGGAATACACAGATTG
GTTAGAATATCTCCTTTTAATGCAAATGGTAAAAGACAGACTTCCTTTGCATCTATAGAAATATTACCAGAACTAACAGA
TGATCAGGAAATAGAAATAAAGAGTGAAGACTTAAAAATAGATACCTATAGAGCAGGTGGAGCTGGAGGACAGCATGTTA
ATAAAACTGAATCTGCAGTAAGAATAACTCATATACCAACAGGAATTATAGTACAATGTCAAAATGAAAGAAGTCAGCAT
ACTAATAAAGAAACAGCTATGAAGGTTTTGAAATCAAAATTAGTAGAACTAAAGGAAAGATCTCATAAAGAAAAAATAGA
AGATTTAACAGGAGAACTAAAGGATATGGGATGGGGAAGTCAAATAAGATCTTATGTATTTCATCCTTATAATTTAGTAA
AAGACCATAGAACAGGAGCAGAAACTAGCAATGTAGATTCAGTGATGAATGGTGATATAAATATATTCATAACACAGTAT
CTAAAAGGAAATTTTTAA

Upstream 100 bases:

>100_bases
ATAAATAATTCCTATGAACTTAAAAATACAATAAAAGAAATCGGGGAGTCTCTTTGACATAGAAAAATTAAAAAATAGTA
TATCAGAATTACAAAATAAA

Downstream 100 bases:

>100_bases
AATTAAAAGTTAAAAAATAAAGGGGATGTCTTAAAATAGATTTAATTTTAACATCATAAAAATAAAAATTAAATCTATTT
TATTTTTAGACATCCCCTTT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MQNPNFWNDIKEAQKITSEEKYLKDKLTKYNHLRNTIEDIISLCEMLSEEDDDMKKEIISEYKNIKEETDRFKIETLLSG
EYDRNNVIMTLHSGVGGTDAQDWTDMLFRMYTRWAEKEGFTIKIMDLLEAEDAGIKSVTLKITGEFVYGYLKGEKGIHRL
VRISPFNANGKRQTSFASIEILPELTDDQEIEIKSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIIVQCQNERSQH
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGAETSNVDSVMNGDINIFITQY
LKGNF

Sequences:

>Translated_325_residues
MQNPNFWNDIKEAQKITSEEKYLKDKLTKYNHLRNTIEDIISLCEMLSEEDDDMKKEIISEYKNIKEETDRFKIETLLSG
EYDRNNVIMTLHSGVGGTDAQDWTDMLFRMYTRWAEKEGFTIKIMDLLEAEDAGIKSVTLKITGEFVYGYLKGEKGIHRL
VRISPFNANGKRQTSFASIEILPELTDDQEIEIKSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIIVQCQNERSQH
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGAETSNVDSVMNGDINIFITQY
LKGNF
>Mature_325_residues
MQNPNFWNDIKEAQKITSEEKYLKDKLTKYNHLRNTIEDIISLCEMLSEEDDDMKKEIISEYKNIKEETDRFKIETLLSG
EYDRNNVIMTLHSGVGGTDAQDWTDMLFRMYTRWAEKEGFTIKIMDLLEAEDAGIKSVTLKITGEFVYGYLKGEKGIHRL
VRISPFNANGKRQTSFASIEILPELTDDQEIEIKSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIIVQCQNERSQH
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGAETSNVDSVMNGDINIFITQY
LKGNF

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=277, Percent_Identity=35.7400722021661, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI34577120, Length=288, Percent_Identity=33.3333333333333, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI166795305, Length=186, Percent_Identity=35.4838709677419, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI2367172, Length=329, Percent_Identity=47.112462006079, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI1787462, Length=326, Percent_Identity=34.6625766871166, Blast_Score=188, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=314, Percent_Identity=28.6624203821656, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=293, Percent_Identity=31.0580204778157, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19921226, Length=282, Percent_Identity=35.4609929078014, Blast_Score=154, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37372; Mature: 37372

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNPNFWNDIKEAQKITSEEKYLKDKLTKYNHLRNTIEDIISLCEMLSEEDDDMKKEIIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EYKNIKEETDRFKIETLLSGEYDRNNVIMTLHSGVGGTDAQDWTDMLFRMYTRWAEKEGF
HHHHHHHHHHHEEHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
TIKIMDLLEAEDAGIKSVTLKITGEFVYGYLKGEKGIHRLVRISPFNANGKRQTSFASIE
EEEEEEHHCCCCCCCEEEEEEEEEEEEEEEECCCHHHHHHEEECCCCCCCCCCCCEEEEE
ILPELTDDQEIEIKSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIIVQCQNERSQH
EECCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHC
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCCCCC
ETSNVDSVMNGDINIFITQYLKGNF
CCCCCHHHHCCCEEEEEEECCCCCC
>Mature Secondary Structure
MQNPNFWNDIKEAQKITSEEKYLKDKLTKYNHLRNTIEDIISLCEMLSEEDDDMKKEIIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EYKNIKEETDRFKIETLLSGEYDRNNVIMTLHSGVGGTDAQDWTDMLFRMYTRWAEKEGF
HHHHHHHHHHHEEHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
TIKIMDLLEAEDAGIKSVTLKITGEFVYGYLKGEKGIHRLVRISPFNANGKRQTSFASIE
EEEEEEHHCCCCCCCEEEEEEEEEEEEEEEECCCHHHHHHEEECCCCCCCCCCCCEEEEE
ILPELTDDQEIEIKSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIIVQCQNERSQH
EECCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHC
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCCCCC
ETSNVDSVMNGDINIFITQYLKGNF
CCCCCHHHHCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA