| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is cphA [H]
Identifier: 226947343
GI number: 226947343
Start: 149596
End: 152217
Strand: Direct
Name: cphA [H]
Synonym: CLM_0163
Alternate gene names: 226947343
Gene position: 149596-152217 (Clockwise)
Preceding gene: 226947342
Following gene: 226947344
Centisome position: 3.6
GC content: 29.41
Gene sequence:
>2622_bases ATGAAAATAGAAAATATAAGAGTATTTGAGGGACGAAATATTTATTCTCATAAAAAATGTATAAGAATGGATGTAGATTT AGAAGGATATAGTAATATATCTAGCAAAGAAATAGAAGGATTTAGCGAGACTCTTTTGAATTATGTTCCAGAATTAAGAG AACATTGCTGTTGTGTAGGTAGAAAAGGTGGCTTTGTAGAAAGACTTTATGAAGGAACTTACTTATCTCATATATGTGAA CACGTAATAATAGCATTACAAAATAGAATTGGTATAGATGTAAGTTATGGTAAAGCAAGAGAAATAGAAGGAGAAAAATA TTATATAATTTATCAATATAAATATAAAAATATGGCTGTTGAGTGTGGAAAGATAGCTGTAGATTTAATCAACAATATAA TCAATGGTAAAAGATATAATATAAGAACTAAAATAAGAGAATTAGCATGTTTATTGAAGACTGAGGAGCTTGGTCCTAGT ACTTTATCCATAATACAAGAAGCTAAAAAAAGAAATATTCCCGTTACTAAAATAGGTGAAGATAGCATGTTTCAATTAGG ATATGGTATCAAAGGAAAAACTATAGAGGCAACTATATGTAATAGTACTAGTGCTGTGTCTGTAGATATAGCTTGTGATA AATTACTATCTAAAAATATATTGATAGATCAGTGTATACCTGTTGCCGAAGGATATAAAGTAAAAAATTATATTGATTTA TTATTTAAAGCAGAAAAATTGGGCTATCCAGTGGTTTTAAAACCGCGTTTTGGGAACCAGGGTAAGGGTGTAGTAGTAAA TATAAAAAACCAAAGGGAGCTAGTAAATGCTTACAGTATAATAAACAAAAAATTCCACGACATAATGATAGAGAAATATA TCAATGGTAAAGATTATAGGGCATGCGTTGTAGATGGAAAGGTGGTAGCTGTAGCACAACGAATTCCACCATACATAATA GGCAATGGTAAAAGTACAATTTATGAATTGATAAAGGAATTAAATAGGGATGAAAGAAGAGGAGATGGACATGAAAAACC CTTAACTAAAGTAAAAATAGATAAAGATCTTAAAAATAATATAAATAAAGAAGGATATACTTTAGGCTATATACTTCCTA AGGGATATAAATTGGAGTTAAGGCATAATGCTAATTTATCTACTGGAGGAGTGGCTATAGATTGTACCGACTTAATATGC AATGAAACAAGAGAGATTTGTGAGAGAGTTGCTAAAGCTATAGGGTTAAACATATGTGGAATAGATATATGTTGTAGTGA TATAAGTAAGCGACTTAAAGAAAATGAAGGTATAATGGAAGTAAATGCAGCACCTGGCATAAGAATGCATCAATATCCCT ATAAAGGGAAAAGCAGAAATGTAGCGAAGGCCATAGTAGATATGATGTTTAAGGAATATGATGGCAATATACCTATTATA TCAATTACAGGAACTAATGGAAAAACTACAACTACTAGGCTGATAGCTCATATTTTAAGTATTTCCGGTAAGAAGGTGGG AATGACAACCACCGGTGGCATATATATAAATAATAAATGTATAAACAAAGGAGATACTACAGGATATTATAGTGCTAAGA CTGTATTAACTAATAAAGAGGTAGAAGTGGCTGTTTTAGAATTAGCAAGAGGAGGATTAATTAAAGCTGGTTTGCCTTAT GATTTGGCGGATGTAGGTATAATAACTAATGTTACAGAGGATCATTTAGGTCTAGGAGATATAAATACTTTAGAAGATAT GGCTTACGTTAAAGCATTAGTAGGTGAAGCTGTTAAAAAAGATGGATATGTAGTAATAAATGCTGATGATGAAGCTAGTA TAAATATTATAAATAGGATGAAAAGCAAAATTATATTATTTACCAAGAATAAAAATAACCCTATTATACCCCAATATTTA GACAATAAAAATTTAGTGTTATATTTAGATGGAGATACCATATATTTAAAGAAAATAAATAAAAATGAGGAAATAATAAA TGTTAATAAAATACCGATAACCTTAGGAGGAAAATTAATTTATAATGTAGAAAATGCAATGGCGGCTATAGCAGCATTAA TAGCATTAGGGTTAGACGTAAATACTATTAGACAAGGTTTAGAAAGTTTTAATAATGAAGAACAAAATCCTGGTAGATTT AATATGTATAATGTACATGGAACAAATGTAATATTAGATTATGGACACAACATTGAAGGATATAAAGTGGTTTTAGAAAG TATTAAAAAAATCAACCATAAAAGAATAATAGGAGTTGTAGGAGTGCCAGGAGATAGAACTAATAGTAGTACGTTAAAAG TAGGTAATATTTGTGGAGAAAACTTCGATTATATATATATAAAGGAAGATAGGGATAAAAGAGGAAGAAAGGATGGAGAA ATAGCAGATTTATTGAAAAAAGGAATTTTAGAAACAGGATTTAAAAATTCTAAGTTAAATATAATATTAGATGAGGAAGA AGCTCTTAAAAAGGCTATAGAATCCTCTAACCCAGGAGATTTAGTTATAATGTTTTTTGAAGAATTTGAACCAGCAGCAA ATATTGTAAAAGATAAAATTAAAAAAGGCAAGATAACAAAACGTGAAACAGCTTTAGCATAG
Upstream 100 bases:
>100_bases TTACATGTACTTAAGAATAGAGATAAGTTTGATTTGAACTATAGAAGGCCTATATAACAAAGCATATATGTTTTTGGAAA AGAGGAGGAAATGTTACAAC
Downstream 100 bases:
>100_bases GATTAAAGCTACAATTTATGAATATCATGATTGTAGCTTTAATTTTTTTGATGTATTATATATTCTATAATATATATTTT TGGAGGAATAAAATGTTATC
Product: cyanophycin synthetase
Products: NA
Alternate protein names: Cyanophycin synthase [H]
Number of amino acids: Translated: 873; Mature: 873
Protein sequence:
>873_residues MKIENIRVFEGRNIYSHKKCIRMDVDLEGYSNISSKEIEGFSETLLNYVPELREHCCCVGRKGGFVERLYEGTYLSHICE HVIIALQNRIGIDVSYGKAREIEGEKYYIIYQYKYKNMAVECGKIAVDLINNIINGKRYNIRTKIRELACLLKTEELGPS TLSIIQEAKKRNIPVTKIGEDSMFQLGYGIKGKTIEATICNSTSAVSVDIACDKLLSKNILIDQCIPVAEGYKVKNYIDL LFKAEKLGYPVVLKPRFGNQGKGVVVNIKNQRELVNAYSIINKKFHDIMIEKYINGKDYRACVVDGKVVAVAQRIPPYII GNGKSTIYELIKELNRDERRGDGHEKPLTKVKIDKDLKNNINKEGYTLGYILPKGYKLELRHNANLSTGGVAIDCTDLIC NETREICERVAKAIGLNICGIDICCSDISKRLKENEGIMEVNAAPGIRMHQYPYKGKSRNVAKAIVDMMFKEYDGNIPII SITGTNGKTTTTRLIAHILSISGKKVGMTTTGGIYINNKCINKGDTTGYYSAKTVLTNKEVEVAVLELARGGLIKAGLPY DLADVGIITNVTEDHLGLGDINTLEDMAYVKALVGEAVKKDGYVVINADDEASINIINRMKSKIILFTKNKNNPIIPQYL DNKNLVLYLDGDTIYLKKINKNEEIINVNKIPITLGGKLIYNVENAMAAIAALIALGLDVNTIRQGLESFNNEEQNPGRF NMYNVHGTNVILDYGHNIEGYKVVLESIKKINHKRIIGVVGVPGDRTNSSTLKVGNICGENFDYIYIKEDRDKRGRKDGE IADLLKKGILETGFKNSKLNIILDEEEALKKAIESSNPGDLVIMFFEEFEPAANIVKDKIKKGKITKRETALA
Sequences:
>Translated_873_residues MKIENIRVFEGRNIYSHKKCIRMDVDLEGYSNISSKEIEGFSETLLNYVPELREHCCCVGRKGGFVERLYEGTYLSHICE HVIIALQNRIGIDVSYGKAREIEGEKYYIIYQYKYKNMAVECGKIAVDLINNIINGKRYNIRTKIRELACLLKTEELGPS TLSIIQEAKKRNIPVTKIGEDSMFQLGYGIKGKTIEATICNSTSAVSVDIACDKLLSKNILIDQCIPVAEGYKVKNYIDL LFKAEKLGYPVVLKPRFGNQGKGVVVNIKNQRELVNAYSIINKKFHDIMIEKYINGKDYRACVVDGKVVAVAQRIPPYII GNGKSTIYELIKELNRDERRGDGHEKPLTKVKIDKDLKNNINKEGYTLGYILPKGYKLELRHNANLSTGGVAIDCTDLIC NETREICERVAKAIGLNICGIDICCSDISKRLKENEGIMEVNAAPGIRMHQYPYKGKSRNVAKAIVDMMFKEYDGNIPII SITGTNGKTTTTRLIAHILSISGKKVGMTTTGGIYINNKCINKGDTTGYYSAKTVLTNKEVEVAVLELARGGLIKAGLPY DLADVGIITNVTEDHLGLGDINTLEDMAYVKALVGEAVKKDGYVVINADDEASINIINRMKSKIILFTKNKNNPIIPQYL DNKNLVLYLDGDTIYLKKINKNEEIINVNKIPITLGGKLIYNVENAMAAIAALIALGLDVNTIRQGLESFNNEEQNPGRF NMYNVHGTNVILDYGHNIEGYKVVLESIKKINHKRIIGVVGVPGDRTNSSTLKVGNICGENFDYIYIKEDRDKRGRKDGE IADLLKKGILETGFKNSKLNIILDEEEALKKAIESSNPGDLVIMFFEEFEPAANIVKDKIKKGKITKRETALA >Mature_873_residues MKIENIRVFEGRNIYSHKKCIRMDVDLEGYSNISSKEIEGFSETLLNYVPELREHCCCVGRKGGFVERLYEGTYLSHICE HVIIALQNRIGIDVSYGKAREIEGEKYYIIYQYKYKNMAVECGKIAVDLINNIINGKRYNIRTKIRELACLLKTEELGPS TLSIIQEAKKRNIPVTKIGEDSMFQLGYGIKGKTIEATICNSTSAVSVDIACDKLLSKNILIDQCIPVAEGYKVKNYIDL LFKAEKLGYPVVLKPRFGNQGKGVVVNIKNQRELVNAYSIINKKFHDIMIEKYINGKDYRACVVDGKVVAVAQRIPPYII GNGKSTIYELIKELNRDERRGDGHEKPLTKVKIDKDLKNNINKEGYTLGYILPKGYKLELRHNANLSTGGVAIDCTDLIC NETREICERVAKAIGLNICGIDICCSDISKRLKENEGIMEVNAAPGIRMHQYPYKGKSRNVAKAIVDMMFKEYDGNIPII SITGTNGKTTTTRLIAHILSISGKKVGMTTTGGIYINNKCINKGDTTGYYSAKTVLTNKEVEVAVLELARGGLIKAGLPY DLADVGIITNVTEDHLGLGDINTLEDMAYVKALVGEAVKKDGYVVINADDEASINIINRMKSKIILFTKNKNNPIIPQYL DNKNLVLYLDGDTIYLKKINKNEEIINVNKIPITLGGKLIYNVENAMAAIAALIALGLDVNTIRQGLESFNNEEQNPGRF NMYNVHGTNVILDYGHNIEGYKVVLESIKKINHKRIIGVVGVPGDRTNSSTLKVGNICGENFDYIYIKEDRDKRGRKDGE IADLLKKGILETGFKNSKLNIILDEEEALKKAIESSNPGDLVIMFFEEFEPAANIVKDKIKKGKITKRETALA
Specific function: Catalyzes the ATP-dependent polymerization of arginine and aspartate to multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer) [H]
COG id: COG1181
COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ATP-grasp domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011761 - InterPro: IPR013651 - InterPro: IPR013815 - InterPro: IPR013816 - InterPro: IPR011810 - InterPro: IPR004101 - InterPro: IPR013221 [H]
Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; PF08443 RimK [H]
EC number: =6.3.2.29; =6.3.2.30 [H]
Molecular weight: Translated: 97437; Mature: 97437
Theoretical pI: Translated: 8.79; Mature: 8.79
Prosite motif: PS50975 ATP_GRASP ; PS01011 FOLYLPOLYGLU_SYNT_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.2 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 3.9 %Cys+Met (Translated Protein) 2.2 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKIENIRVFEGRNIYSHKKCIRMDVDLEGYSNISSKEIEGFSETLLNYVPELREHCCCVG CCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHEEC RKGGFVERLYEGTYLSHICEHVIIALQNRIGIDVSYGKAREIEGEKYYIIYQYKYKNMAV CCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCEEEEEEEEEECCCCH ECGKIAVDLINNIINGKRYNIRTKIRELACLLKTEELGPSTLSIIQEAKKRNIPVTKIGE HHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECC DSMFQLGYGIKGKTIEATICNSTSAVSVDIACDKLLSKNILIDQCIPVAEGYKVKNYIDL CCEEEECCCCCCCEEEEEEECCCCEEEEEHHHHHHHHCCCCHHHCCCCCCCEEHHHHHHH LFKAEKLGYPVVLKPRFGNQGKGVVVNIKNQRELVNAYSIINKKFHDIMIEKYINGKDYR HHHHHHCCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE ACVVDGKVVAVAQRIPPYIIGNGKSTIYELIKELNRDERRGDGHEKPLTKVKIDKDLKNN EEEECCEEEEEHHHCCCEEEECCHHHHHHHHHHHCCHHHCCCCCCCCCEEEEECCHHHCC INKEGYTLGYILPKGYKLELRHNANLSTGGVAIDCTDLICNETREICERVAKAIGLNICG CCCCCEEEEEEECCCEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEE IDICCSDISKRLKENEGIMEVNAAPGIRMHQYPYKGKSRNVAKAIVDMMFKEYDGNIPII HHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEE SITGTNGKTTTTRLIAHILSISGKKVGMTTTGGIYINNKCINKGDTTGYYSAKTVLTNKE EEECCCCCHHHHHHHHHHHHCCCCEECEEECCCEEECCEECCCCCCCCEEEEEEEEECCC VEVAVLELARGGLIKAGLPYDLADVGIITNVTEDHLGLGDINTLEDMAYVKALVGEAVKK CEEEEEEHHCCCEEECCCCCCHHCCCEEECCCHHCCCCCCCCHHHHHHHHHHHHHHHHHC DGYVVINADDEASINIINRMKSKIILFTKNKNNPIIPQYLDNKNLVLYLDGDTIYLKKIN CCEEEEECCCCCHHHHHHHCCCEEEEEEECCCCCCCCCEECCCCEEEEECCCEEEEEEEC KNEEIINVNKIPITLGGKLIYNVENAMAAIAALIALGLDVNTIRQGLESFNNEEQNPGRF CCCCEEEEEEEEEEECCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCE NMYNVHGTNVILDYGHNIEGYKVVLESIKKINHKRIIGVVGVPGDRTNSSTLKVGNICGE EEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCC NFDYIYIKEDRDKRGRKDGEIADLLKKGILETGFKNSKLNIILDEEEALKKAIESSNPGD CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCC LVIMFFEEFEPAANIVKDKIKKGKITKRETALA EEEEEEHHCCHHHHHHHHHHHCCCCCCHHHCCC >Mature Secondary Structure MKIENIRVFEGRNIYSHKKCIRMDVDLEGYSNISSKEIEGFSETLLNYVPELREHCCCVG CCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHEEC RKGGFVERLYEGTYLSHICEHVIIALQNRIGIDVSYGKAREIEGEKYYIIYQYKYKNMAV CCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCEEEEEEEEEECCCCH ECGKIAVDLINNIINGKRYNIRTKIRELACLLKTEELGPSTLSIIQEAKKRNIPVTKIGE HHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECC DSMFQLGYGIKGKTIEATICNSTSAVSVDIACDKLLSKNILIDQCIPVAEGYKVKNYIDL CCEEEECCCCCCCEEEEEEECCCCEEEEEHHHHHHHHCCCCHHHCCCCCCCEEHHHHHHH LFKAEKLGYPVVLKPRFGNQGKGVVVNIKNQRELVNAYSIINKKFHDIMIEKYINGKDYR HHHHHHCCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE ACVVDGKVVAVAQRIPPYIIGNGKSTIYELIKELNRDERRGDGHEKPLTKVKIDKDLKNN EEEECCEEEEEHHHCCCEEEECCHHHHHHHHHHHCCHHHCCCCCCCCCEEEEECCHHHCC INKEGYTLGYILPKGYKLELRHNANLSTGGVAIDCTDLICNETREICERVAKAIGLNICG CCCCCEEEEEEECCCEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEE IDICCSDISKRLKENEGIMEVNAAPGIRMHQYPYKGKSRNVAKAIVDMMFKEYDGNIPII HHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEE SITGTNGKTTTTRLIAHILSISGKKVGMTTTGGIYINNKCINKGDTTGYYSAKTVLTNKE EEECCCCCHHHHHHHHHHHHCCCCEECEEECCCEEECCEECCCCCCCCEEEEEEEEECCC VEVAVLELARGGLIKAGLPYDLADVGIITNVTEDHLGLGDINTLEDMAYVKALVGEAVKK CEEEEEEHHCCCEEECCCCCCHHCCCEEECCCHHCCCCCCCCHHHHHHHHHHHHHHHHHC DGYVVINADDEASINIINRMKSKIILFTKNKNNPIIPQYLDNKNLVLYLDGDTIYLKKIN CCEEEEECCCCCHHHHHHHCCCEEEEEEECCCCCCCCCEECCCCEEEEECCCEEEEEEEC KNEEIINVNKIPITLGGKLIYNVENAMAAIAALIALGLDVNTIRQGLESFNNEEQNPGRF CCCCEEEEEEEEEEECCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCE NMYNVHGTNVILDYGHNIEGYKVVLESIKKINHKRIIGVVGVPGDRTNSSTLKVGNICGE EEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCC NFDYIYIKEDRDKRGRKDGEIADLLKKGILETGFKNSKLNIILDEEEALKKAIESSNPGD CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCC LVIMFFEEFEPAANIVKDKIKKGKITKRETALA EEEEEEHHCCHHHHHHHHHHHCCCCCCHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8905231 [H]