Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 226225361

GI number: 226225361

Start: 2862691

End: 2864064

Strand: Reverse

Name: trmE

Synonym: Lm4b_02784

Alternate gene names: 226225361

Gene position: 2864064-2862691 (Counterclockwise)

Preceding gene: 226225363

Following gene: 226225360

Centisome position: 98.33

GC content: 42.87

Gene sequence:

>1374_bases
ATGGAATTTGATACTATTGCTGCAATCTCAACACCGCCGGGAGAAGGGGCCATTGCTATTATTAGATTAAGCGGTCCAGA
GGCGATTCAGATAGCGGATCGTATTTTTTATGCCAAAAATAGCTTAAGTGAAGCAGAAAGTCACACCATTCACTATGGTC
ATATAAAAGAAGACGGCGAAGTAATTGAGGAAGTAATGGTCACAGTTATGCGTGCACCTCGTACTTTTACACGGGAAGAT
GTGGTAGAAATCAACGCGCACGGCGGAATTGTTTCCGTGAACCGCGTGTTGCAACTGTTACTTCGAAATGGGGCTAATTT
AGCTGAACCAGGTGAATTCACAAAGCGCGCATTTTTAAATGGAAGAATTGATTTGTCGCAAGCGGAGGCTGTGATGGATT
TAATTCGCGCCAAGACAGACCGGGCGATGGGCGTGGCAATTCGGCAAATGGACGGAAATCTTTCACGGTTGATTCGCAAT
TTACGTCAAGAAATTTTGGATGCACTAGCGCAAGTCGAAGTAAATATCGACTATCCGGAGTACGATGATGTGGAGGAAAT
GACCCAGCGAATGCTACTCGAGAAAACCGAGCTAGTTCGCGCTTCCGTCGAGCAACTTTTACAAACAGCAAGCCAAGGGA
AAATTCTTCGTGAAGGCCTAGCAACGGCTATTATCGGTCGACCAAATGTTGGGAAATCATCTTTGCTTAACCAATTAATT
CAAGAAGAAAAAGCGATTGTCACAGATATTGCTGGGACGACGCGAGATATTATAGAAGAATACGTCAATGTTCGTGGTGT
TCCGCTGCGTTTAATCGATACAGCTGGAATTCGTGAGACAGAAGATATTGTGGAAAAAATTGGTGTAGAACGTTCAAGAA
AAGCCTTGGCGGATGCCGATTTTATCCTGCTAGTATTAAATCAAAACGAAGAGCTGACAGTGGAAGATGAAGCGTTATTT
GAAGCAGCCGCGGGTCATAATTATGTGGTCGTTTTAAATAAAACAGATTTGGAAACAAAGCTTGATATAAATAGAGTGCG
TGAACTTGCGGGAGAAAACCCGATTGTTTCCACTTCTCTAGTAAACGATGAAGGTTTAGAGGCTTTAGAAGAAGCAATTA
AAACACTATTTTTTGCTGGTGACATTGATGCGGGTGATGCAACATATGTATCGAACGTTCGACACATTGCGCTCCTTCAC
CAAGCACTTGAAGCTTTAAATGGCGTTACAACAGGGATTCAGCTCGGTATGCCAGTGGATATTGTCCAGATTGATATGAC
TCGTGCATGGGATTTACTAGGTGAAATTACTGGTGATTCTGTCCAGGATGAATTACTCGATCAGTTGTTCAATCAATTTT
GCCTTGGAAAATGA

Upstream 100 bases:

>100_bases
GTAAGTTGTTGAAAATGCACAAAATCATGTGCCAAAATTTATTCAAAATAGGATAAGGCTTATTTGAGCTGAAATTCTAA
TGAGAAAGGATGAGTAAAGA

Downstream 100 bases:

>100_bases
TTTTTTAGGAGATGTTTTTATAAAATGCAAACCTATGATGCAGGAACTTTTGACGTCATCGTTGTTGGTGCAGGCCATGC
GGGAGTAGAAGCTGGTCTTG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MEFDTIAAISTPPGEGAIAIIRLSGPEAIQIADRIFYAKNSLSEAESHTIHYGHIKEDGEVIEEVMVTVMRAPRTFTRED
VVEINAHGGIVSVNRVLQLLLRNGANLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMGVAIRQMDGNLSRLIRN
LRQEILDALAQVEVNIDYPEYDDVEEMTQRMLLEKTELVRASVEQLLQTASQGKILREGLATAIIGRPNVGKSSLLNQLI
QEEKAIVTDIAGTTRDIIEEYVNVRGVPLRLIDTAGIRETEDIVEKIGVERSRKALADADFILLVLNQNEELTVEDEALF
EAAAGHNYVVVLNKTDLETKLDINRVRELAGENPIVSTSLVNDEGLEALEEAIKTLFFAGDIDAGDATYVSNVRHIALLH
QALEALNGVTTGIQLGMPVDIVQIDMTRAWDLLGEITGDSVQDELLDQLFNQFCLGK

Sequences:

>Translated_457_residues
MEFDTIAAISTPPGEGAIAIIRLSGPEAIQIADRIFYAKNSLSEAESHTIHYGHIKEDGEVIEEVMVTVMRAPRTFTRED
VVEINAHGGIVSVNRVLQLLLRNGANLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMGVAIRQMDGNLSRLIRN
LRQEILDALAQVEVNIDYPEYDDVEEMTQRMLLEKTELVRASVEQLLQTASQGKILREGLATAIIGRPNVGKSSLLNQLI
QEEKAIVTDIAGTTRDIIEEYVNVRGVPLRLIDTAGIRETEDIVEKIGVERSRKALADADFILLVLNQNEELTVEDEALF
EAAAGHNYVVVLNKTDLETKLDINRVRELAGENPIVSTSLVNDEGLEALEEAIKTLFFAGDIDAGDATYVSNVRHIALLH
QALEALNGVTTGIQLGMPVDIVQIDMTRAWDLLGEITGDSVQDELLDQLFNQFCLGK
>Mature_457_residues
MEFDTIAAISTPPGEGAIAIIRLSGPEAIQIADRIFYAKNSLSEAESHTIHYGHIKEDGEVIEEVMVTVMRAPRTFTRED
VVEINAHGGIVSVNRVLQLLLRNGANLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMGVAIRQMDGNLSRLIRN
LRQEILDALAQVEVNIDYPEYDDVEEMTQRMLLEKTELVRASVEQLLQTASQGKILREGLATAIIGRPNVGKSSLLNQLI
QEEKAIVTDIAGTTRDIIEEYVNVRGVPLRLIDTAGIRETEDIVEKIGVERSRKALADADFILLVLNQNEELTVEDEALF
EAAAGHNYVVVLNKTDLETKLDINRVRELAGENPIVSTSLVNDEGLEALEEAIKTLFFAGDIDAGDATYVSNVRHIALLH
QALEALNGVTTGIQLGMPVDIVQIDMTRAWDLLGEITGDSVQDELLDQLFNQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=472, Percent_Identity=35.3813559322034, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI193082993, Length=460, Percent_Identity=35, Blast_Score=215, Evalue=7e-56,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=33.4, Blast_Score=209, Evalue=5e-54,
Organism=Escherichia coli, GI2367268, Length=463, Percent_Identity=34.341252699784, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI87082120, Length=119, Percent_Identity=42.0168067226891, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=28.5714285714286, Blast_Score=171, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6323665, Length=489, Percent_Identity=29.2433537832311, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI45550813, Length=475, Percent_Identity=31.1578947368421, Blast_Score=200, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50386; Mature: 50386

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFDTIAAISTPPGEGAIAIIRLSGPEAIQIADRIFYAKNSLSEAESHTIHYGHIKEDGE
CCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCEEECCCCCHHH
VIEEVMVTVMRAPRTFTREDVVEINAHGGIVSVNRVLQLLLRNGANLAEPGEFTKRAFLN
HHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHC
GRIDLSQAEAVMDLIRAKTDRAMGVAIRQMDGNLSRLIRNLRQEILDALAQVEVNIDYPE
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECCCCC
YDDVEEMTQRMLLEKTELVRASVEQLLQTASQGKILREGLATAIIGRPNVGKSSLLNQLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
QEEKAIVTDIAGTTRDIIEEYVNVRGVPLRLIDTAGIRETEDIVEKIGVERSRKALADAD
HHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHCCCC
FILLVLNQNEELTVEDEALFEAAAGHNYVVVLNKTDLETKLDINRVRELAGENPIVSTSL
EEEEEECCCCCEEECHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEC
VNDEGLEALEEAIKTLFFAGDIDAGDATYVSNVRHIALLHQALEALNGVTTGIQLGMPVD
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCEECCCCEE
IVQIDMTRAWDLLGEITGDSVQDELLDQLFNQFCLGK
EEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFDTIAAISTPPGEGAIAIIRLSGPEAIQIADRIFYAKNSLSEAESHTIHYGHIKEDGE
CCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCEEECCCCCHHH
VIEEVMVTVMRAPRTFTREDVVEINAHGGIVSVNRVLQLLLRNGANLAEPGEFTKRAFLN
HHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHC
GRIDLSQAEAVMDLIRAKTDRAMGVAIRQMDGNLSRLIRNLRQEILDALAQVEVNIDYPE
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECCCCC
YDDVEEMTQRMLLEKTELVRASVEQLLQTASQGKILREGLATAIIGRPNVGKSSLLNQLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
QEEKAIVTDIAGTTRDIIEEYVNVRGVPLRLIDTAGIRETEDIVEKIGVERSRKALADAD
HHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHCCCC
FILLVLNQNEELTVEDEALFEAAAGHNYVVVLNKTDLETKLDINRVRELAGENPIVSTSL
EEEEEECCCCCEEECHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEC
VNDEGLEALEEAIKTLFFAGDIDAGDATYVSNVRHIALLHQALEALNGVTTGIQLGMPVD
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCEECCCCEE
IVQIDMTRAWDLLGEITGDSVQDELLDQLFNQFCLGK
EEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]