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The map label for this gene is gidA

Identifier: 226225360

GI number: 226225360

Start: 2860777

End: 2862666

Strand: Reverse

Name: gidA

Synonym: Lm4b_02783

Alternate gene names: 226225360

Gene position: 2862666-2860777 (Counterclockwise)

Preceding gene: 226225361

Following gene: 226225359

Centisome position: 98.28

GC content: 42.22

Gene sequence:

>1890_bases
ATGCAAACCTATGATGCAGGAACTTTTGACGTCATCGTTGTTGGTGCAGGCCATGCGGGAGTAGAAGCTGGTCTTGCCAG
TGGTCGAATGGGCGCAAAAACACTGATGTTAACGATAAATTTAGATATGGTCGCTTTTATGCCATGTAATCCTTCTGTTG
GAGGGCCAGCAAAAGGCGTGGTTGTGCGCGAAATTGATGCTCTTGGTGGCGAAATGGGTCGCAACACGGATAAAACGTAC
ATCCAAATGCGGATGCTAAATACAGGTAAAGGTCCCGCTGTTCGCGCATTACGTGCTCAAGCAGACAAATGGGATTATCA
ACACGAAATGAAACACACAATTGAAAAAGAAGAAAATATCACTTTGCGCCAAGGACTTGTTGACCGTTTAGTGATTGAAG
ATGGCGTGTGTAAAGGCGTTATTACAAACAGTGGCGCAATTTACTACGCGAAAACAGTTGTTATTACTACCGGAACTTTC
TCACGTGGCGAAATTATTGTCGGCGAACTGCGCTATTCCAGCGGTCCAAACAACCAACAACCTTCTGTGAAACTTTCAGA
GCATCTGGAAGAACTAGGTTTTGAACTACGTCGCTTTAAAACAGGAACACCCCCGCGTGTAAAATCAAGTACGATTGACT
ACTCAAAAACAGAAGAACAACCGGGTGACGACCATCCGCGAGCATTTAGCTTTGATACGGTGGAAATGTTGCTTGACCAA
CTACCGTGTTGGTTAACTTATACCAACGAAACAACCCACGAAATAATCCAAGCGAACTTACACCGTTCACCAATGTTTAC
AGCAACGAAAAAAGGCACTGGCGCAAGATATTGCCCATCCATTGAAGATAAAATTGTTCGTTTCAGCGACAAACCAAGAC
ACCAAATCTTCCTTGAACCAGAAGGCAAAAATACCGAGGAAGTATATGTCCAAGGCCTTTCCACCAGTTTGCCAGAAGAA
GTGCAACGCGAAATGCTAAGAACTATTCCGGGACTTGAAAATGTAGAAATGATGCGTGTAGGCTACGCTATCGAATATGA
TGCAGTAATGCCAGACCAACTGTGGCCATCGCTTGAAACTAAATTAGTCGAAGGTCTTTTCACAGCCGGCCAAATTAATG
GTACAAGTGGTTACGAAGAAGCAGCAGGACAAGGATTAATGGCAGGAATCAATGCCGCACGTAAAGTCTTTGAAAAAGAA
CCAGTTATCCTTGGTCGTGACCAAGCTTATATCGGCGTTTTAATTGACGATTTAGTAACAAAAGGAACTGAAGAACCATA
TCGTTTACTTACATCTCGTGCCGAATACCGTTTGCTATTACGCCATGATAATGCAGATTTACGTTTAACAGAAATCGGTC
ATGAAATCGGCTTAATTAGCGACGAACGTTATGAACGCTTTTTAGCAAAACAAAGTGCCATCGAAGCAGAAAAAGAAAGA
CTACAAAAAACACGCATTAAACCAACTGCTGAAGTCCAAGCAATGCTAAAAGAAATCGGTTCTGGAGAGCTAAAAGACGG
AATTTTAGCAGCAGATTTACTCCGCCGTCCCGAAATAACTTACGACAAAATCGCCCAAATTGTTTCACGTGAAACATTTG
TGACCGACGAAATCGCTGAACAAGTAGAAATTCAAATTAAATACGAAGGCTACATTCAAAAATCCAACCTTCAAGTAGAA
AAAATGAAGCGCATGGAAGACAAGAAAATCCCAGAAAATATCGATTATGATGCAATCAGCGGCCTAGCAACAGAAGCCCT
AGAAAAATTAAAGAAAATCGAACCCCTTTCCATCGCCCAAGCGAGCCGTATCAGCGGAGTAAACCCAGCCGATATATCTA
TTTTGCTAGTTTATATTGAGCAAGGAAAGATAGCTAAAATAAGTAAATAA

Upstream 100 bases:

>100_bases
AGGTGAAATTACTGGTGATTCTGTCCAGGATGAATTACTCGATCAGTTGTTCAATCAATTTTGCCTTGGAAAATGATTTT
TTAGGAGATGTTTTTATAAA

Downstream 100 bases:

>100_bases
GAACACGGATTACTATAATTACTGAAACCACATGTTCACGAAAACTTCAAACTTACCGTTTAAGTTGAAAGTTTCCGCTG
AATAATGTGGTTTTTTCTTG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MQTYDAGTFDVIVVGAGHAGVEAGLASGRMGAKTLMLTINLDMVAFMPCNPSVGGPAKGVVVREIDALGGEMGRNTDKTY
IQMRMLNTGKGPAVRALRAQADKWDYQHEMKHTIEKEENITLRQGLVDRLVIEDGVCKGVITNSGAIYYAKTVVITTGTF
SRGEIIVGELRYSSGPNNQQPSVKLSEHLEELGFELRRFKTGTPPRVKSSTIDYSKTEEQPGDDHPRAFSFDTVEMLLDQ
LPCWLTYTNETTHEIIQANLHRSPMFTATKKGTGARYCPSIEDKIVRFSDKPRHQIFLEPEGKNTEEVYVQGLSTSLPEE
VQREMLRTIPGLENVEMMRVGYAIEYDAVMPDQLWPSLETKLVEGLFTAGQINGTSGYEEAAGQGLMAGINAARKVFEKE
PVILGRDQAYIGVLIDDLVTKGTEEPYRLLTSRAEYRLLLRHDNADLRLTEIGHEIGLISDERYERFLAKQSAIEAEKER
LQKTRIKPTAEVQAMLKEIGSGELKDGILAADLLRRPEITYDKIAQIVSRETFVTDEIAEQVEIQIKYEGYIQKSNLQVE
KMKRMEDKKIPENIDYDAISGLATEALEKLKKIEPLSIAQASRISGVNPADISILLVYIEQGKIAKISK

Sequences:

>Translated_629_residues
MQTYDAGTFDVIVVGAGHAGVEAGLASGRMGAKTLMLTINLDMVAFMPCNPSVGGPAKGVVVREIDALGGEMGRNTDKTY
IQMRMLNTGKGPAVRALRAQADKWDYQHEMKHTIEKEENITLRQGLVDRLVIEDGVCKGVITNSGAIYYAKTVVITTGTF
SRGEIIVGELRYSSGPNNQQPSVKLSEHLEELGFELRRFKTGTPPRVKSSTIDYSKTEEQPGDDHPRAFSFDTVEMLLDQ
LPCWLTYTNETTHEIIQANLHRSPMFTATKKGTGARYCPSIEDKIVRFSDKPRHQIFLEPEGKNTEEVYVQGLSTSLPEE
VQREMLRTIPGLENVEMMRVGYAIEYDAVMPDQLWPSLETKLVEGLFTAGQINGTSGYEEAAGQGLMAGINAARKVFEKE
PVILGRDQAYIGVLIDDLVTKGTEEPYRLLTSRAEYRLLLRHDNADLRLTEIGHEIGLISDERYERFLAKQSAIEAEKER
LQKTRIKPTAEVQAMLKEIGSGELKDGILAADLLRRPEITYDKIAQIVSRETFVTDEIAEQVEIQIKYEGYIQKSNLQVE
KMKRMEDKKIPENIDYDAISGLATEALEKLKKIEPLSIAQASRISGVNPADISILLVYIEQGKIAKISK
>Mature_629_residues
MQTYDAGTFDVIVVGAGHAGVEAGLASGRMGAKTLMLTINLDMVAFMPCNPSVGGPAKGVVVREIDALGGEMGRNTDKTY
IQMRMLNTGKGPAVRALRAQADKWDYQHEMKHTIEKEENITLRQGLVDRLVIEDGVCKGVITNSGAIYYAKTVVITTGTF
SRGEIIVGELRYSSGPNNQQPSVKLSEHLEELGFELRRFKTGTPPRVKSSTIDYSKTEEQPGDDHPRAFSFDTVEMLLDQ
LPCWLTYTNETTHEIIQANLHRSPMFTATKKGTGARYCPSIEDKIVRFSDKPRHQIFLEPEGKNTEEVYVQGLSTSLPEE
VQREMLRTIPGLENVEMMRVGYAIEYDAVMPDQLWPSLETKLVEGLFTAGQINGTSGYEEAAGQGLMAGINAARKVFEKE
PVILGRDQAYIGVLIDDLVTKGTEEPYRLLTSRAEYRLLLRHDNADLRLTEIGHEIGLISDERYERFLAKQSAIEAEKER
LQKTRIKPTAEVQAMLKEIGSGELKDGILAADLLRRPEITYDKIAQIVSRETFVTDEIAEQVEIQIKYEGYIQKSNLQVE
KMKRMEDKKIPENIDYDAISGLATEALEKLKKIEPLSIAQASRISGVNPADISILLVYIEQGKIAKISK

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=629, Percent_Identity=41.4944356120827, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI19882217, Length=654, Percent_Identity=39.9082568807339, Blast_Score=444, Evalue=1e-124,
Organism=Homo sapiens, GI183227703, Length=669, Percent_Identity=39.0134529147982, Blast_Score=438, Evalue=1e-123,
Organism=Escherichia coli, GI2367273, Length=615, Percent_Identity=51.0569105691057, Blast_Score=628, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=617, Percent_Identity=39.3841166936791, Blast_Score=452, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6321202, Length=619, Percent_Identity=42.3263327948304, Blast_Score=466, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24658174, Length=638, Percent_Identity=41.8495297805643, Blast_Score=461, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70138; Mature: 70138

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTYDAGTFDVIVVGAGHAGVEAGLASGRMGAKTLMLTINLDMVAFMPCNPSVGGPAKGV
CCCCCCCCEEEEEEECCCCCHHHHHCCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCE
VVREIDALGGEMGRNTDKTYIQMRMLNTGKGPAVRALRAQADKWDYQHEMKHTIEKEENI
EEEEHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
TLRQGLVDRLVIEDGVCKGVITNSGAIYYAKTVVITTGTFSRGEIIVGELRYSSGPNNQQ
CHHHHHHHHHHHCCCCCCEEEECCCEEEEEEEEEEEECCCCCCCEEEEEEEECCCCCCCC
PSVKLSEHLEELGFELRRFKTGTPPRVKSSTIDYSKTEEQPGDDHPRAFSFDTVEMLLDQ
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHC
LPCWLTYTNETTHEIIQANLHRSPMFTATKKGTGARYCPSIEDKIVRFSDKPRHQIFLEP
CCCEEEECCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHEECCCCCCEEEEECC
EGKNTEEVYVQGLSTSLPEEVQREMLRTIPGLENVEMMRVGYAIEYDAVMPDQLWPSLET
CCCCCHHHEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECEEEEECCCCCHHHCHHHHH
KLVEGLFTAGQINGTSGYEEAAGQGLMAGINAARKVFEKEPVILGRDQAYIGVLIDDLVT
HHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCEEECCCCEEHHHHHHHHH
KGTEEPYRLLTSRAEYRLLLRHDNADLRLTEIGHEIGLISDERYERFLAKQSAIEAEKER
CCCCCHHHHHHCCCCEEEEEEECCCCEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHH
LQKTRIKPTAEVQAMLKEIGSGELKDGILAADLLRRPEITYDKIAQIVSRETFVTDEIAE
HHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
QVEIQIKYEGYIQKSNLQVEKMKRMEDKKIPENIDYDAISGLATEALEKLKKIEPLSIAQ
CEEEEEEEECEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
ASRISGVNPADISILLVYIEQGKIAKISK
HHHCCCCCCCCEEEEEEEEECCCEECCCC
>Mature Secondary Structure
MQTYDAGTFDVIVVGAGHAGVEAGLASGRMGAKTLMLTINLDMVAFMPCNPSVGGPAKGV
CCCCCCCCEEEEEEECCCCCHHHHHCCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCE
VVREIDALGGEMGRNTDKTYIQMRMLNTGKGPAVRALRAQADKWDYQHEMKHTIEKEENI
EEEEHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
TLRQGLVDRLVIEDGVCKGVITNSGAIYYAKTVVITTGTFSRGEIIVGELRYSSGPNNQQ
CHHHHHHHHHHHCCCCCCEEEECCCEEEEEEEEEEEECCCCCCCEEEEEEEECCCCCCCC
PSVKLSEHLEELGFELRRFKTGTPPRVKSSTIDYSKTEEQPGDDHPRAFSFDTVEMLLDQ
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHC
LPCWLTYTNETTHEIIQANLHRSPMFTATKKGTGARYCPSIEDKIVRFSDKPRHQIFLEP
CCCEEEECCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHEECCCCCCEEEEECC
EGKNTEEVYVQGLSTSLPEEVQREMLRTIPGLENVEMMRVGYAIEYDAVMPDQLWPSLET
CCCCCHHHEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECEEEEECCCCCHHHCHHHHH
KLVEGLFTAGQINGTSGYEEAAGQGLMAGINAARKVFEKEPVILGRDQAYIGVLIDDLVT
HHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCEEECCCCEEHHHHHHHHH
KGTEEPYRLLTSRAEYRLLLRHDNADLRLTEIGHEIGLISDERYERFLAKQSAIEAEKER
CCCCCHHHHHHCCCCEEEEEEECCCCEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHH
LQKTRIKPTAEVQAMLKEIGSGELKDGILAADLLRRPEITYDKIAQIVSRETFVTDEIAE
HHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
QVEIQIKYEGYIQKSNLQVEKMKRMEDKKIPENIDYDAISGLATEALEKLKKIEPLSIAQ
CEEEEEEEECEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
ASRISGVNPADISILLVYIEQGKIAKISK
HHHCCCCCCCCEEEEEEEEECCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA