Click here to switch to the map view.

The map label for this gene is hrsA [H]

Identifier: 226225284

GI number: 226225284

Start: 2775431

End: 2777389

Strand: Direct

Name: hrsA [H]

Synonym: Lm4b_02707

Alternate gene names: 226225284

Gene position: 2775431-2777389 (Clockwise)

Preceding gene: 226225280

Following gene: 226225285

Centisome position: 95.29

GC content: 39.61

Gene sequence:

>1959_bases
TTGAAATTAAGCGATATTACATCGGAGCCACTCATCTTTTTAGATAAAGATTGGCGCACTAAAGAAGAAGTATTTGATGG
ATTAATTGATGCTTTACACAATCAAGGAGTTTTGTCAGATAAAAATGAATTCAAAAAAGCTGTCTTAGAACGCGAAAAGA
TTTCTGAAACTGGTTTAGAGAAAGGTTTTGCTATTCCACATGGTAAATCTAAAGCCGTTAAAAAAGCTGCTTTTGCATTT
GCTCGTATCAATCAACCAATTAATTCTTGGGGCAGCATTGATCCAACAAATGAAGTAAAATATATCTTTTTATTAGCTAT
TCCAGAAAACGAAGCCGGATCAACTCATTTAGAACTTTTGGCAGCTTTAAGTAAGCGTTTACTCGACCCTGCTTTTATTG
AGAGCATTTCTACCGCTCCGACAGTAAACGAATTTATAACCGTGCTAGATTCTGAACAACAAGAAGATGAAAAAGTAGAT
TATACACGAAAAATCGTTGCTATTACAGCATGTGCAGCGGGTATCGCCCATACGTATATGGCAGCGGAAGCCCTTGAACT
AGCTGGTAAAGAACTTGGTATTGAAGTTATCGTCGAAAAACAAGGTGCGAATGGTATTGAAGACCGCATTACTCCTGAAA
TCCTTAAAGGCGCAGAAGCAGTCATTTTCGCGACAGATATTACCGCAAAAGATAAAGAACGTTTCGCCGGAATGCCCTTT
ATCCAAAGACGTGTCGCTGAGCCACTTCGTAGTGGTAAAGAAATGATTGAAACTGTATTAAGCAAACCAGATGGCTATGT
CAAAGCTGATACAGATGGTTCAGAACAACAATTTGATAATAATAAGAGCAGTGTTTTCTCGCAAATTTATCGTGGGATTT
TAACTGGTATTTCTTATATGTTACCTGTTATTGTCGCAGGTGGTTTAATGATTGGTATTGGCCAACTTGGCGCAACTGCC
TTTGGTCTTGAAAAAGTTATTGGTAATCCAGAGTATGCAACAAACTCTAACCAGCTTATTGTAATCTTCCATTATCTCGG
TTTATACGGAAATATGATTATGAAATTCATGTATCCTGTTTTCGGTGCCTTCCTTGCTTACTCAATTGCTGACCGTCCTG
GACTTGCTCCCGGGTTTATCGGTGGTGCTTTCGCAGCAGGGCTACATTACACCTTCTGGGGCATAGATGGCGGTATACCA
TCTGGATTCTTCGGTGTTCTAATTTTAGGTATCATTGCCGGATTTGTTGCTAAATTCTTAAACGAAAAAATTAAATTACA
CAAAAATTTACAAGCTATGAAACCAATGTTCCTGATTCCAGGGATTACCGTTTTAGTCTTGTTCTTCGTCAATTACTATG
TGGTCGACCCGGTATTCGGGGGCTTAAACGCATGGTTACAAGAACTAATTATTCAAAATCAAGATGCAAGTGCTGTACTA
CTTTCGACAATTATTGCTTGTTTGACTGCATTTGACTTAGGTGGACCAGTCAATAAAGCAGCTGGAGCTATCGCAATCGG
ACTCGCAGCAGACGGAATTTTCCCGCTAACTGCTCGTGTTTTAGCAATCGTTATCCCGCCAATCGGACTTGGGCTTGCGA
CTGTATTAGATAAATATGTTGTTAAACGTCGTGTTTTTGATGAAAACTTACGTGTTGCAGGTACAACCTCTATTTTCCTA
GGGTTTATTGCCATAGGAGAGGGCGCGATACCGTTTATGCTACAAAATCCACTTATCACTATTCCAATTAATATGATTGG
TGCTTCTCTTGGTGCGGTTACTGCCGTTTTACTTGGTGCAGTACAATGGCTTCCACTTCCAGCAATTTGGGGTTGGCCGC
TCGTTGAAAATTTCTGGGCCTATGCGATTGGTTTAATCGTAGGTATCGCCTTTATCACATTCGCGAATATCTTCGTACGC
TACGGCTTAATCACACGTAAAGAAAATAAAATCAAATAA

Upstream 100 bases:

>100_bases
ACTGCGTACAATTGTACGATTTTCGTACTTTTTCAAAAAAATAATATGGTATAACTAAAGTAACCTAGGGAAAAGACTGT
TAATAAAGGAGCTGATAGCA

Downstream 100 bases:

>100_bases
AAAAATAGGCTGACAGGTACTCTCGTTAGTACCTGTCACTTAATTTGAGAGGTTTTTAAAATGAAAAAAAATAAAATTGT
ACATGTTGTCGCACATTCCC

Product: PTS system, fructose-specific IIABC component

Products: NA

Alternate protein names: Putative PTS system EIIABC component; Phosphotransferase enzyme IIA component; PTS system EIIA component; Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MKLSDITSEPLIFLDKDWRTKEEVFDGLIDALHNQGVLSDKNEFKKAVLEREKISETGLEKGFAIPHGKSKAVKKAAFAF
ARINQPINSWGSIDPTNEVKYIFLLAIPENEAGSTHLELLAALSKRLLDPAFIESISTAPTVNEFITVLDSEQQEDEKVD
YTRKIVAITACAAGIAHTYMAAEALELAGKELGIEVIVEKQGANGIEDRITPEILKGAEAVIFATDITAKDKERFAGMPF
IQRRVAEPLRSGKEMIETVLSKPDGYVKADTDGSEQQFDNNKSSVFSQIYRGILTGISYMLPVIVAGGLMIGIGQLGATA
FGLEKVIGNPEYATNSNQLIVIFHYLGLYGNMIMKFMYPVFGAFLAYSIADRPGLAPGFIGGAFAAGLHYTFWGIDGGIP
SGFFGVLILGIIAGFVAKFLNEKIKLHKNLQAMKPMFLIPGITVLVLFFVNYYVVDPVFGGLNAWLQELIIQNQDASAVL
LSTIIACLTAFDLGGPVNKAAGAIAIGLAADGIFPLTARVLAIVIPPIGLGLATVLDKYVVKRRVFDENLRVAGTTSIFL
GFIAIGEGAIPFMLQNPLITIPINMIGASLGAVTAVLLGAVQWLPLPAIWGWPLVENFWAYAIGLIVGIAFITFANIFVR
YGLITRKENKIK

Sequences:

>Translated_652_residues
MKLSDITSEPLIFLDKDWRTKEEVFDGLIDALHNQGVLSDKNEFKKAVLEREKISETGLEKGFAIPHGKSKAVKKAAFAF
ARINQPINSWGSIDPTNEVKYIFLLAIPENEAGSTHLELLAALSKRLLDPAFIESISTAPTVNEFITVLDSEQQEDEKVD
YTRKIVAITACAAGIAHTYMAAEALELAGKELGIEVIVEKQGANGIEDRITPEILKGAEAVIFATDITAKDKERFAGMPF
IQRRVAEPLRSGKEMIETVLSKPDGYVKADTDGSEQQFDNNKSSVFSQIYRGILTGISYMLPVIVAGGLMIGIGQLGATA
FGLEKVIGNPEYATNSNQLIVIFHYLGLYGNMIMKFMYPVFGAFLAYSIADRPGLAPGFIGGAFAAGLHYTFWGIDGGIP
SGFFGVLILGIIAGFVAKFLNEKIKLHKNLQAMKPMFLIPGITVLVLFFVNYYVVDPVFGGLNAWLQELIIQNQDASAVL
LSTIIACLTAFDLGGPVNKAAGAIAIGLAADGIFPLTARVLAIVIPPIGLGLATVLDKYVVKRRVFDENLRVAGTTSIFL
GFIAIGEGAIPFMLQNPLITIPINMIGASLGAVTAVLLGAVQWLPLPAIWGWPLVENFWAYAIGLIVGIAFITFANIFVR
YGLITRKENKIK
>Mature_652_residues
MKLSDITSEPLIFLDKDWRTKEEVFDGLIDALHNQGVLSDKNEFKKAVLEREKISETGLEKGFAIPHGKSKAVKKAAFAF
ARINQPINSWGSIDPTNEVKYIFLLAIPENEAGSTHLELLAALSKRLLDPAFIESISTAPTVNEFITVLDSEQQEDEKVD
YTRKIVAITACAAGIAHTYMAAEALELAGKELGIEVIVEKQGANGIEDRITPEILKGAEAVIFATDITAKDKERFAGMPF
IQRRVAEPLRSGKEMIETVLSKPDGYVKADTDGSEQQFDNNKSSVFSQIYRGILTGISYMLPVIVAGGLMIGIGQLGATA
FGLEKVIGNPEYATNSNQLIVIFHYLGLYGNMIMKFMYPVFGAFLAYSIADRPGLAPGFIGGAFAAGLHYTFWGIDGGIP
SGFFGVLILGIIAGFVAKFLNEKIKLHKNLQAMKPMFLIPGITVLVLFFVNYYVVDPVFGGLNAWLQELIIQNQDASAVL
LSTIIACLTAFDLGGPVNKAAGAIAIGLAADGIFPLTARVLAIVIPPIGLGLATVLDKYVVKRRVFDENLRVAGTTSIFL
GFIAIGEGAIPFMLQNPLITIPINMIGASLGAVTAVLLGAVQWLPLPAIWGWPLVENFWAYAIGLIVGIAFITFANIFVR
YGLITRKENKIK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786951, Length=608, Percent_Identity=36.1842105263158, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1788492, Length=448, Percent_Identity=36.3839285714286, Blast_Score=248, Evalue=1e-66,
Organism=Escherichia coli, GI1788729, Length=410, Percent_Identity=34.390243902439, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI87082348, Length=508, Percent_Identity=30.9055118110236, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1790386, Length=347, Percent_Identity=32.8530259365994, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI48994992, Length=147, Percent_Identity=29.9319727891156, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790387, Length=104, Percent_Identity=42.3076923076923, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788730, Length=98, Percent_Identity=35.7142857142857, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI1788726, Length=141, Percent_Identity=31.2056737588652, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1790390, Length=102, Percent_Identity=38.2352941176471, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 70640; Mature: 70640

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSDITSEPLIFLDKDWRTKEEVFDGLIDALHNQGVLSDKNEFKKAVLEREKISETGLE
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
KGFAIPHGKSKAVKKAAFAFARINQPINSWGSIDPTNEVKYIFLLAIPENEAGSTHLELL
CCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHH
AALSKRLLDPAFIESISTAPTVNEFITVLDSEQQEDEKVDYTRKIVAITACAAGIAHTYM
HHHHHHCCCHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAEALELAGKELGIEVIVEKQGANGIEDRITPEILKGAEAVIFATDITAKDKERFAGMPF
HHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCCCCHHHHHCCCHH
IQRRVAEPLRSGKEMIETVLSKPDGYVKADTDGSEQQFDNNKSSVFSQIYRGILTGISYM
HHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
LPVIVAGGLMIGIGQLGATAFGLEKVIGNPEYATNSNQLIVIFHYLGLYGNMIMKFMYPV
HHHHHHCHHHEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
FGAFLAYSIADRPGLAPGFIGGAFAAGLHYTFWGIDGGIPSGFFGVLILGIIAGFVAKFL
HHHHHHHHHHCCCCCCCCHHHHHHHHCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
NEKIKLHKNLQAMKPMFLIPGITVLVLFFVNYYVVDPVFGGLNAWLQELIIQNQDASAVL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHH
LSTIIACLTAFDLGGPVNKAAGAIAIGLAADGIFPLTARVLAIVIPPIGLGLATVLDKYV
HHHHHHHHHHHHCCCCCHHHCCCEEEEHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
VKRRVFDENLRVAGTTSIFLGFIAIGEGAIPFMLQNPLITIPINMIGASLGAVTAVLLGA
HHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCCEECCCEEEEEHHHHHHHHHHHHHHHHHH
VQWLPLPAIWGWPLVENFWAYAIGLIVGIAFITFANIFVRYGLITRKENKIK
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLSDITSEPLIFLDKDWRTKEEVFDGLIDALHNQGVLSDKNEFKKAVLEREKISETGLE
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
KGFAIPHGKSKAVKKAAFAFARINQPINSWGSIDPTNEVKYIFLLAIPENEAGSTHLELL
CCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHH
AALSKRLLDPAFIESISTAPTVNEFITVLDSEQQEDEKVDYTRKIVAITACAAGIAHTYM
HHHHHHCCCHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAEALELAGKELGIEVIVEKQGANGIEDRITPEILKGAEAVIFATDITAKDKERFAGMPF
HHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCCCCHHHHHCCCHH
IQRRVAEPLRSGKEMIETVLSKPDGYVKADTDGSEQQFDNNKSSVFSQIYRGILTGISYM
HHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
LPVIVAGGLMIGIGQLGATAFGLEKVIGNPEYATNSNQLIVIFHYLGLYGNMIMKFMYPV
HHHHHHCHHHEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
FGAFLAYSIADRPGLAPGFIGGAFAAGLHYTFWGIDGGIPSGFFGVLILGIIAGFVAKFL
HHHHHHHHHHCCCCCCCCHHHHHHHHCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
NEKIKLHKNLQAMKPMFLIPGITVLVLFFVNYYVVDPVFGGLNAWLQELIIQNQDASAVL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHH
LSTIIACLTAFDLGGPVNKAAGAIAIGLAADGIFPLTARVLAIVIPPIGLGLATVLDKYV
HHHHHHHHHHHHCCCCCHHHCCCEEEEHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
VKRRVFDENLRVAGTTSIFLGFIAIGEGAIPFMLQNPLITIPINMIGASLGAVTAVLLGA
HHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCCEECCCEEEEEHHHHHHHHHHHHHHHHHH
VQWLPLPAIWGWPLVENFWAYAIGLIVGIAFITFANIFVRYGLITRKENKIK
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9063979; 9278503; 8905232 [H]