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The map label for this gene is mepA [H]

Identifier: 226225276

GI number: 226225276

Start: 2769601

End: 2770953

Strand: Reverse

Name: mepA [H]

Synonym: Lm4b_02699

Alternate gene names: 226225276

Gene position: 2770953-2769601 (Counterclockwise)

Preceding gene: 226225277

Following gene: 226225275

Centisome position: 95.13

GC content: 41.83

Gene sequence:

>1353_bases
ATGAAAGAACAGAGTAAACGATTAGGTGAAGATAGCATTCCTTCACTCATGGCGAGATTGTCGATACCGGCATTTATCGG
CATGTTTGTTATGGGGATGTATAATATTGTTGATACGATTTTCGTGTCATATGGCGTTGGGCCATCAGGGGTTGCAGCAC
TTTCAATTGCATTCCCAGTTCAAATGATACTAATGGCAATGGCAGCTATGTTTGGTATTGGTGGCGCGTCGATTATTTCC
CGCTCGCTCGGTGCTGGGGAACAAAAACAAGCGGATAAAGTATTCCATCAAGTTATTTGGTTAGTACTTATTTCCAGCAT
TTTTATTGCAATTGTAACCTTTATTTTTTTAGATCCGCTTATTACGCTTTTCGGAGCACCGGCAGATATTCATGATATTG
CCAGTGATTTCTTATCGCTTATTTTGTTAGGCGCAGTGTTCCAAACCTTCGCGATGGCGATGAATAATATCGTTCGCTCA
GAAGGTAATGCGAAAACAGCGATGTTAACCATGATTATTTCCGCTATTTTAAATATGATTTTAAACCCAATTTTCATTAT
GGGCTTTGGTATGGGCGTTCGTGGTTCTGCCCTTGCAACTGTTATTGCGCAGGCTGTTGGCGCGATTTGGCTCTTGATTT
ATTTTTTATCAGGAAAAAGTACTTTATCTTTAAAAGGGTTCTCATTCCGAATGGATTTCCCGCTGATTCGCCGGATTATG
GCAATCGGGTTCCCGTCATTTATTATGATGTCCGCAGGTAGTATCGTAACGGTTGCGGTAAACTGGATGCTTAATATTTA
TGGTGGAACGATGGCGATTGCAGTATACGGAATTGCGAACCGGATTGCGTCGTTCGTCATCATGCCAATCAATGGTGTCA
CACAAGGGATGCAACCAATCGTCGGCTTCAACTACGGTTCCAGACAATTCGAACGCGTAATGAAAGCTGTTAAAGTATCA
ATGATAGCTGCAACTGTCATGTCACTAATTGCTTGGGGATTGGTAGAAATTTTCCCAGGAATGCTCGTACGAATTTTCTC
TAACGACCCAGAACTGATTGCACAAGGAACAAATGCTGTTAGATTTATGCTACTAGCTGCGCCAACAATTGGTTTCCAAA
TTGTCTGCGGTGGGCTATATCAAGCACTTGGTCGAGCAAGAATTTCATTCATCATTTCCCTAATGCGCCAAATCATCTGT
TTAGTACCACTTTTACTTATTTTGCCGCAGTTCTTCGGTTTAGATGGTATTTGGTACGCCTTCCCATTAGCAGATTTAGG
CGCGTTCACCGTCTGTCTAGTGATTATGAGCAAAACATGGCGCCGAATTTTTAAAAATCCCGAGATAGTTTAA

Upstream 100 bases:

>100_bases
TAACAGCGAATTTTAGTGAAAAGGAAAAAGATGACTTAATGCATTTGATAGGACGACTTTCAGATAATGCGATTATAGCA
GTCGAAGGAGGAGAATCTGA

Downstream 100 bases:

>100_bases
GGTAAAACGAATTGGGTATTTTCTAACTAGTGTCTCAAAAACAAAGGAGCGATTTTTATGGCAAAAATGTACCCGTATTT
GGCTTTTGAAAATGCAAAAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKEQSKRLGEDSIPSLMARLSIPAFIGMFVMGMYNIVDTIFVSYGVGPSGVAALSIAFPVQMILMAMAAMFGIGGASIIS
RSLGAGEQKQADKVFHQVIWLVLISSIFIAIVTFIFLDPLITLFGAPADIHDIASDFLSLILLGAVFQTFAMAMNNIVRS
EGNAKTAMLTMIISAILNMILNPIFIMGFGMGVRGSALATVIAQAVGAIWLLIYFLSGKSTLSLKGFSFRMDFPLIRRIM
AIGFPSFIMMSAGSIVTVAVNWMLNIYGGTMAIAVYGIANRIASFVIMPINGVTQGMQPIVGFNYGSRQFERVMKAVKVS
MIAATVMSLIAWGLVEIFPGMLVRIFSNDPELIAQGTNAVRFMLLAAPTIGFQIVCGGLYQALGRARISFIISLMRQIIC
LVPLLLILPQFFGLDGIWYAFPLADLGAFTVCLVIMSKTWRRIFKNPEIV

Sequences:

>Translated_450_residues
MKEQSKRLGEDSIPSLMARLSIPAFIGMFVMGMYNIVDTIFVSYGVGPSGVAALSIAFPVQMILMAMAAMFGIGGASIIS
RSLGAGEQKQADKVFHQVIWLVLISSIFIAIVTFIFLDPLITLFGAPADIHDIASDFLSLILLGAVFQTFAMAMNNIVRS
EGNAKTAMLTMIISAILNMILNPIFIMGFGMGVRGSALATVIAQAVGAIWLLIYFLSGKSTLSLKGFSFRMDFPLIRRIM
AIGFPSFIMMSAGSIVTVAVNWMLNIYGGTMAIAVYGIANRIASFVIMPINGVTQGMQPIVGFNYGSRQFERVMKAVKVS
MIAATVMSLIAWGLVEIFPGMLVRIFSNDPELIAQGTNAVRFMLLAAPTIGFQIVCGGLYQALGRARISFIISLMRQIIC
LVPLLLILPQFFGLDGIWYAFPLADLGAFTVCLVIMSKTWRRIFKNPEIV
>Mature_450_residues
MKEQSKRLGEDSIPSLMARLSIPAFIGMFVMGMYNIVDTIFVSYGVGPSGVAALSIAFPVQMILMAMAAMFGIGGASIIS
RSLGAGEQKQADKVFHQVIWLVLISSIFIAIVTFIFLDPLITLFGAPADIHDIASDFLSLILLGAVFQTFAMAMNNIVRS
EGNAKTAMLTMIISAILNMILNPIFIMGFGMGVRGSALATVIAQAVGAIWLLIYFLSGKSTLSLKGFSFRMDFPLIRRIM
AIGFPSFIMMSAGSIVTVAVNWMLNIYGGTMAIAVYGIANRIASFVIMPINGVTQGMQPIVGFNYGSRQFERVMKAVKVS
MIAATVMSLIAWGLVEIFPGMLVRIFSNDPELIAQGTNAVRFMLLAAPTIGFQIVCGGLYQALGRARISFIISLMRQIIC
LVPLLLILPQFFGLDGIWYAFPLADLGAFTVCLVIMSKTWRRIFKNPEIV

Specific function: Multidrug resistance efflux protein [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MepA subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510954, Length=445, Percent_Identity=22.6966292134831, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR015522 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 48866; Mature: 48866

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
6.9 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
6.9 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEQSKRLGEDSIPSLMARLSIPAFIGMFVMGMYNIVDTIFVSYGVGPSGVAALSIAFPV
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
QMILMAMAAMFGIGGASIISRSLGAGEQKQADKVFHQVIWLVLISSIFIAIVTFIFLDPL
HHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITLFGAPADIHDIASDFLSLILLGAVFQTFAMAMNNIVRSEGNAKTAMLTMIISAILNMI
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LNPIFIMGFGMGVRGSALATVIAQAVGAIWLLIYFLSGKSTLSLKGFSFRMDFPLIRRIM
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHH
AIGFPSFIMMSAGSIVTVAVNWMLNIYGGTMAIAVYGIANRIASFVIMPINGVTQGMQPI
HHCCHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECCCHHHHCCCHH
VGFNYGSRQFERVMKAVKVSMIAATVMSLIAWGLVEIFPGMLVRIFSNDPELIAQGTNAV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHH
RFMLLAAPTIGFQIVCGGLYQALGRARISFIISLMRQIICLVPLLLILPQFFGLDGIWYA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FPLADLGAFTVCLVIMSKTWRRIFKNPEIV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKEQSKRLGEDSIPSLMARLSIPAFIGMFVMGMYNIVDTIFVSYGVGPSGVAALSIAFPV
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
QMILMAMAAMFGIGGASIISRSLGAGEQKQADKVFHQVIWLVLISSIFIAIVTFIFLDPL
HHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITLFGAPADIHDIASDFLSLILLGAVFQTFAMAMNNIVRSEGNAKTAMLTMIISAILNMI
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LNPIFIMGFGMGVRGSALATVIAQAVGAIWLLIYFLSGKSTLSLKGFSFRMDFPLIRRIM
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHH
AIGFPSFIMMSAGSIVTVAVNWMLNIYGGTMAIAVYGIANRIASFVIMPINGVTQGMQPI
HHCCHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECCCHHHHCCCHH
VGFNYGSRQFERVMKAVKVSMIAATVMSLIAWGLVEIFPGMLVRIFSNDPELIAQGTNAV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHH
RFMLLAAPTIGFQIVCGGLYQALGRARISFIISLMRQIICLVPLLLILPQFFGLDGIWYA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FPLADLGAFTVCLVIMSKTWRRIFKNPEIV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA