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The map label for this gene is ytcI [H]

Identifier: 226225271

GI number: 226225271

Start: 2765413

End: 2766990

Strand: Direct

Name: ytcI [H]

Synonym: Lm4b_02694

Alternate gene names: 226225271

Gene position: 2765413-2766990 (Clockwise)

Preceding gene: 226225261

Following gene: 226225273

Centisome position: 94.94

GC content: 43.35

Gene sequence:

>1578_bases
ATGAATTCAGAAAATTTAATCGCTCCAGAACTTTATAATATTACCGACGAGATTGCTAAATTCAGTTCTGAAAAAACTGC
TTTAATTTGGAAAAATGAGCATAACGAAACAAAAACTTGGAGTTACCGCCACCTGCTTGAACAAGCGAATAAATTTGCCA
ATGTTGTCAAAGACGCAGGCATAAAAAAAGGTGACCACGTTATCGTCATGACACCTAGATTACTCGAAACGTACGCAATT
TACATGGGCTTATGGAAAGCTGGTGCCATTATCATCCCGGCATCCGAGCTACTTAAAGCGCACGATTTAGAATACCGCAT
CCACCATGCAAACGTAAAAGCAATCGTTTCTTACAATGGAATGACTGCTGAATTTGATAAAATTGAAAGCATCCCTTCTG
TTTCGAAAAAAATTATTGTTGGGGAGAAATTATCCGGCTGGGATCACTACGAAACATTAATGGAAGCCGCTTCAACCGAA
TTTGAACGCGTGGAAACTTCGCGCGATGATGCTTGTCTGCTTGCATTCACGAGTGGAACAACAGGTAATCCTAAAGGCGT
TGTACATATTCACGGCTGGGGTTACGCGCATATTCGTATCGCTGCTGACCATTGGCTCGATATTCACGAAGACGATATCG
TTTGGGCCACAGCTGGTCCTGGTTGGCAAAAATGGGTTTGGTCTCCTTTCCTTTCTGTACTCGGAAAAGGTGCCACTGGC
TTCATTTACAACGGTCGTTTCATTCCTGAAAAACAACTTCATTTACTGGAAGAAGAAAAAATCAACGTGCTGTGCTGTAC
GCCAACAGAATATCGCTTAATGGCTAAAGTAAATAATTTGCGCGAACATGATTTAAGTTCCCTTCGTAGCGCCGTATCAG
CAGGAGAACCTCTAAACCGCGAAGTAATTCAAGTTTTCCAAGATAATTTTGATATTAAAGTTCGCGACGGCTACGGTCAA
ACGGAGAGTACACTGTTGATTGGGACACTGGTTGATACGCCGATTCGCCCTGGTTCGATGGGTAAACCGATTATGCCAGA
ATACATGGCAATTATTGACGCTGAAGGAAATCCAGTTGGTGTCGGTGAAATTGGTGATATCGCGATGCGTAGAGATTTCC
CAGCACTTTTCAAAGAGTACTATAAAGAACCAGAACGTTTGCAAAAAGCGATTCGCGGTGATTATTTCGTTTCTGGTGAC
CGCGCTATCCGCGATGAAGATAATTATTACTGGTTCCAAGGCCGGAATGACGATATTATTATTAGCTCTGGTTACACGAT
TGGACCGTTCGAAGTAGAGGACGCGCTCACACATCATCCAGCCGTAAAAGAAGTCGCTGTTGTCGCAAGTCCTGACGAAA
TCCGCGGTACGGTCGTAAAAGCATTTATCGTCTTAAAGGATGGTTACGAGGGCACGGAAGATCTTGTGCATGAATTACAA
ACATTCACGAAAGAACAAACCGCTCCGTATAAGTATCCGCGCCGGATTGAGTTTGTTGAGGCATTACCGAAAACTGATTC
TGGTAAAATTCGTCGCGTTGAGTTGCGTGATGCGGAGTTTGCTAGTGTGCATGGATAA

Upstream 100 bases:

>100_bases
TTTAGTTGCTTTCGCGGAACATTTATATTACCATATATTAATACTAGGTCCTAAAACAAAATTATCAAAATATACAATCT
ACTTATAAAGGAGAGTCATT

Downstream 100 bases:

>100_bases
TCAAAAACCCAGCCTTCCTAAATAGAAGGTTGGGTTTTCTTTTAAATATTCAACTCACTCGCCGCTTCCTCGTCCAGCAC
CACTGTAAAATTAGGGTGTA

Product: acetate-CoA ligase

Products: AMP; diphosphate; acetyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MNSENLIAPELYNITDEIAKFSSEKTALIWKNEHNETKTWSYRHLLEQANKFANVVKDAGIKKGDHVIVMTPRLLETYAI
YMGLWKAGAIIIPASELLKAHDLEYRIHHANVKAIVSYNGMTAEFDKIESIPSVSKKIIVGEKLSGWDHYETLMEAASTE
FERVETSRDDACLLAFTSGTTGNPKGVVHIHGWGYAHIRIAADHWLDIHEDDIVWATAGPGWQKWVWSPFLSVLGKGATG
FIYNGRFIPEKQLHLLEEEKINVLCCTPTEYRLMAKVNNLREHDLSSLRSAVSAGEPLNREVIQVFQDNFDIKVRDGYGQ
TESTLLIGTLVDTPIRPGSMGKPIMPEYMAIIDAEGNPVGVGEIGDIAMRRDFPALFKEYYKEPERLQKAIRGDYFVSGD
RAIRDEDNYYWFQGRNDDIIISSGYTIGPFEVEDALTHHPAVKEVAVVASPDEIRGTVVKAFIVLKDGYEGTEDLVHELQ
TFTKEQTAPYKYPRRIEFVEALPKTDSGKIRRVELRDAEFASVHG

Sequences:

>Translated_525_residues
MNSENLIAPELYNITDEIAKFSSEKTALIWKNEHNETKTWSYRHLLEQANKFANVVKDAGIKKGDHVIVMTPRLLETYAI
YMGLWKAGAIIIPASELLKAHDLEYRIHHANVKAIVSYNGMTAEFDKIESIPSVSKKIIVGEKLSGWDHYETLMEAASTE
FERVETSRDDACLLAFTSGTTGNPKGVVHIHGWGYAHIRIAADHWLDIHEDDIVWATAGPGWQKWVWSPFLSVLGKGATG
FIYNGRFIPEKQLHLLEEEKINVLCCTPTEYRLMAKVNNLREHDLSSLRSAVSAGEPLNREVIQVFQDNFDIKVRDGYGQ
TESTLLIGTLVDTPIRPGSMGKPIMPEYMAIIDAEGNPVGVGEIGDIAMRRDFPALFKEYYKEPERLQKAIRGDYFVSGD
RAIRDEDNYYWFQGRNDDIIISSGYTIGPFEVEDALTHHPAVKEVAVVASPDEIRGTVVKAFIVLKDGYEGTEDLVHELQ
TFTKEQTAPYKYPRRIEFVEALPKTDSGKIRRVELRDAEFASVHG
>Mature_525_residues
MNSENLIAPELYNITDEIAKFSSEKTALIWKNEHNETKTWSYRHLLEQANKFANVVKDAGIKKGDHVIVMTPRLLETYAI
YMGLWKAGAIIIPASELLKAHDLEYRIHHANVKAIVSYNGMTAEFDKIESIPSVSKKIIVGEKLSGWDHYETLMEAASTE
FERVETSRDDACLLAFTSGTTGNPKGVVHIHGWGYAHIRIAADHWLDIHEDDIVWATAGPGWQKWVWSPFLSVLGKGATG
FIYNGRFIPEKQLHLLEEEKINVLCCTPTEYRLMAKVNNLREHDLSSLRSAVSAGEPLNREVIQVFQDNFDIKVRDGYGQ
TESTLLIGTLVDTPIRPGSMGKPIMPEYMAIIDAEGNPVGVGEIGDIAMRRDFPALFKEYYKEPERLQKAIRGDYFVSGD
RAIRDEDNYYWFQGRNDDIIISSGYTIGPFEVEDALTHHPAVKEVAVVASPDEIRGTVVKAFIVLKDGYEGTEDLVHELQ
TFTKEQTAPYKYPRRIEFVEALPKTDSGKIRRVELRDAEFASVHG

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=534, Percent_Identity=36.8913857677903, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI122937307, Length=539, Percent_Identity=38.0333951762523, Blast_Score=353, Evalue=3e-97,
Organism=Homo sapiens, GI38505220, Length=537, Percent_Identity=37.6163873370577, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI58082049, Length=507, Percent_Identity=38.0670611439842, Blast_Score=324, Evalue=2e-88,
Organism=Homo sapiens, GI157311624, Length=507, Percent_Identity=37.67258382643, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI157311622, Length=507, Percent_Identity=37.67258382643, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI115511026, Length=531, Percent_Identity=34.8399246704331, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI8923896, Length=570, Percent_Identity=28.9473684210526, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI28416953, Length=544, Percent_Identity=29.4117647058824, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI253970489, Length=582, Percent_Identity=28.1786941580756, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI42544134, Length=348, Percent_Identity=31.0344827586207, Blast_Score=192, Evalue=9e-49,
Organism=Homo sapiens, GI195947378, Length=402, Percent_Identity=28.1094527363184, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI13375727, Length=554, Percent_Identity=25.4512635379061, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI156151445, Length=523, Percent_Identity=27.151051625239, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI187761345, Length=518, Percent_Identity=26.4478764478764, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI187761343, Length=518, Percent_Identity=26.4478764478764, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1790505, Length=537, Percent_Identity=29.4227188081937, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1788107, Length=515, Percent_Identity=26.6019417475728, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI221142682, Length=526, Percent_Identity=26.0456273764259, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1786529, Length=553, Percent_Identity=27.8481012658228, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI145693145, Length=498, Percent_Identity=29.5180722891566, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1786810, Length=522, Percent_Identity=24.904214559387, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789201, Length=381, Percent_Identity=26.246719160105, Blast_Score=92, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71982997, Length=555, Percent_Identity=27.7477477477477, Blast_Score=181, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI71983001, Length=555, Percent_Identity=27.7477477477477, Blast_Score=181, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI17558820, Length=482, Percent_Identity=25.9336099585062, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17559526, Length=531, Percent_Identity=25.8003766478343, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32563687, Length=518, Percent_Identity=25.2895752895753, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=529, Percent_Identity=27.0321361058601, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71994694, Length=540, Percent_Identity=25.5555555555556, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=540, Percent_Identity=25.5555555555556, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=540, Percent_Identity=25.5555555555556, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=529, Percent_Identity=24.1965973534972, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=499, Percent_Identity=22.2444889779559, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=531, Percent_Identity=23.5404896421846, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=522, Percent_Identity=23.5632183908046, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=510, Percent_Identity=24.3137254901961, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17551278, Length=535, Percent_Identity=23.9252336448598, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=389, Percent_Identity=26.2210796915167, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323182, Length=567, Percent_Identity=29.1005291005291, Blast_Score=193, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6319264, Length=557, Percent_Identity=27.1095152603232, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6319699, Length=358, Percent_Identity=26.8156424581006, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24667955, Length=557, Percent_Identity=27.2890484739677, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI62472339, Length=528, Percent_Identity=27.8409090909091, Blast_Score=185, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24581924, Length=517, Percent_Identity=26.8858800773694, Blast_Score=162, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24667959, Length=362, Percent_Identity=30.3867403314917, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21355181, Length=494, Percent_Identity=25.3036437246964, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI21355225, Length=519, Percent_Identity=23.6994219653179, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI18859661, Length=506, Percent_Identity=24.1106719367589, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24656500, Length=493, Percent_Identity=22.920892494929, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922652, Length=494, Percent_Identity=23.2793522267206, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648260, Length=391, Percent_Identity=25.0639386189258, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648257, Length=503, Percent_Identity=23.6580516898608, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24653035, Length=388, Percent_Identity=23.9690721649485, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356947, Length=408, Percent_Identity=23.5294117647059, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648676, Length=559, Percent_Identity=22.1824686940966, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161076582, Length=400, Percent_Identity=22.5, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281364798, Length=545, Percent_Identity=24.4036697247706, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356441, Length=358, Percent_Identity=24.0223463687151, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583465, Length=545, Percent_Identity=24.4036697247706, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583463, Length=545, Percent_Identity=24.4036697247706, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20130357, Length=403, Percent_Identity=24.8138957816377, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648253, Length=483, Percent_Identity=22.9813664596273, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648255, Length=483, Percent_Identity=22.9813664596273, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.1

Molecular weight: Translated: 59276; Mature: 59276

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSENLIAPELYNITDEIAKFSSEKTALIWKNEHNETKTWSYRHLLEQANKFANVVKDAG
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
IKKGDHVIVMTPRLLETYAIYMGLWKAGAIIIPASELLKAHDLEYRIHHANVKAIVSYNG
CCCCCEEEEECHHHHHHHHHHHHHHHCCEEEEEHHHHHHHCCCEEEEEECCEEEEEEECC
MTAEFDKIESIPSVSKKIIVGEKLSGWDHYETLMEAASTEFERVETSRDDACLLAFTSGT
CCCCHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHCCHHHHHHCCCCCEEEEEEECCC
TGNPKGVVHIHGWGYAHIRIAADHWLDIHEDDIVWATAGPGWQKWVWSPFLSVLGKGATG
CCCCCEEEEEECCCEEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCE
FIYNGRFIPEKQLHLLEEEKINVLCCTPTEYRLMAKVNNLREHDLSSLRSAVSAGEPLNR
EEECCEECCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EVIQVFQDNFDIKVRDGYGQTESTLLIGTLVDTPIRPGSMGKPIMPEYMAIIDAEGNPVG
HHHHHHHCCCCEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEC
VGEIGDIAMRRDFPALFKEYYKEPERLQKAIRGDYFVSGDRAIRDEDNYYWFQGRNDDII
CCCCCCHHHHCCCHHHHHHHCCCHHHHHHHHCCCEEECCCCEEECCCCEEEEECCCCCEE
ISSGYTIGPFEVEDALTHHPAVKEVAVVASPDEIRGTVVKAFIVLKDGYEGTEDLVHELQ
EECCCEECCEEHHHHHHCCCCHHHEEEEECCHHHHHHEEEEEEEEECCCCCHHHHHHHHH
TFTKEQTAPYKYPRRIEFVEALPKTDSGKIRRVELRDAEFASVHG
HHHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCHHCCCC
>Mature Secondary Structure
MNSENLIAPELYNITDEIAKFSSEKTALIWKNEHNETKTWSYRHLLEQANKFANVVKDAG
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
IKKGDHVIVMTPRLLETYAIYMGLWKAGAIIIPASELLKAHDLEYRIHHANVKAIVSYNG
CCCCCEEEEECHHHHHHHHHHHHHHHCCEEEEEHHHHHHHCCCEEEEEECCEEEEEEECC
MTAEFDKIESIPSVSKKIIVGEKLSGWDHYETLMEAASTEFERVETSRDDACLLAFTSGT
CCCCHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHCCHHHHHHCCCCCEEEEEEECCC
TGNPKGVVHIHGWGYAHIRIAADHWLDIHEDDIVWATAGPGWQKWVWSPFLSVLGKGATG
CCCCCEEEEEECCCEEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCE
FIYNGRFIPEKQLHLLEEEKINVLCCTPTEYRLMAKVNNLREHDLSSLRSAVSAGEPLNR
EEECCEECCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EVIQVFQDNFDIKVRDGYGQTESTLLIGTLVDTPIRPGSMGKPIMPEYMAIIDAEGNPVG
HHHHHHHCCCCEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEC
VGEIGDIAMRRDFPALFKEYYKEPERLQKAIRGDYFVSGDRAIRDEDNYYWFQGRNDDII
CCCCCCHHHHCCCHHHHHHHCCCHHHHHHHHCCCEEECCCCEEECCCCEEEEECCCCCEE
ISSGYTIGPFEVEDALTHHPAVKEVAVVASPDEIRGTVVKAFIVLKDGYEGTEDLVHELQ
EECCCEECCEEHHHHHHCCCCHHHEEEEECCHHHHHHEEEEEEEEECCCCCHHHHHHHHH
TFTKEQTAPYKYPRRIEFVEALPKTDSGKIRRVELRDAEFASVHG
HHHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; acetate; CoA

Specific reaction: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA

General reaction: Acid-thiol ligation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]