Click here to switch to the map view.

The map label for this gene is nrdF

Identifier: 226224761

GI number: 226224761

Start: 2236797

End: 2237846

Strand: Reverse

Name: nrdF

Synonym: Lm4b_02179

Alternate gene names: 226224761

Gene position: 2237846-2236797 (Counterclockwise)

Preceding gene: 226224762

Following gene: 226224760

Centisome position: 76.83

GC content: 36.19

Gene sequence:

>1050_bases
ATGGCTAACCAAAAAGAACAACTGACACGTATTAAAATTTTAGAACCTTTATTTCCTAATCGTTCTACTTCAATCATAAA
CGGAGAAACTAGTGGGATTTTAAATTGGAATGACATCCCGTATCCTTCTTTCTACCGTGCGTATAAAGAACTTTCTACTA
ACTACTGGATTCCAGACGAAGTAGACATGAAAAGCGATGCAAAACAATATCCCGCTCTTTCAGAACAAGAAAAATATGCT
TTTGATGCAATCATTGGCTTACTTGCAACACTGGATTCTCCGCAAACACGTTTTATTTATAATATTGCGGAATATATTAC
CGATCCAGCTGTTCATGCAAACGCAGCGATTATCGCGCAACAAGAAGTCATTCATAATGAGAGTTATTCCTATGTACTTG
CATCTATTACCAATTTACAAGAACAAAATCGCGTATTTGAACTTGCGAGAACACATCCGACAATCATCAAACGTAATGAG
CCAATCATGGACGCATATGATGACTTCATGAATAACAAAACAGGTGAAACACTTGTAAAAGCATTAATTCAATCTTCTAT
TTTAGAAGGAATTAATTTCTATAGTGGTTTTGCTTACTTCTACAATCTTGTTCGTCAAAATAAAATGACTGGAACAGGGA
AAATAATTAGCTTTATCAATCGTGATGAACTTGCTCACTCGAAGTTCATTTCAGAAGTAATCCGGGCAATCCTTGGTGAA
AACCCAGAATTACAAACAGAAGAGCTAGTAGAATACACACATGATGCTTTCCGCCACGCTGTTGAACTTGAAACAGAATG
GTCTGAAGAAGTGTTACAAGGTATTGAAGGTATTGATGTGGAAGAAATGGTTGACTACGTGAAATATCGGGCCAACAAAA
TGCTAGGAATGCTTGGTATTCCAGAACTTTATCCAGGACATAGTGATAACACAATGACATGGATTAAAGCTTACGCAGAT
AACTTTACAGAAACAAAAACCGACTTTTTCGAAATGCGCAATTCAAGCTATAAAAAAACGAATATGGATAACGGATTCGA
CGATTTATGA

Upstream 100 bases:

>100_bases
TCTAACGATATCGATGTTGAAGAGTGCGAATGGTGCTCAAGCTGATACATCTCTAAAATACTTTACACTTAGTACAAAAT
GAAAGGGAGGGCTTGCCTAG

Downstream 100 bases:

>100_bases
GAATTTTGTTAGCCTATGATTCTCTAAGTGGTAATACGAAAATGGTGGCTGATGAAATTGAAGAGATTTTACAAAGCGAA
GGACATGAGGTTGTGTCCTT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MANQKEQLTRIKILEPLFPNRSTSIINGETSGILNWNDIPYPSFYRAYKELSTNYWIPDEVDMKSDAKQYPALSEQEKYA
FDAIIGLLATLDSPQTRFIYNIAEYITDPAVHANAAIIAQQEVIHNESYSYVLASITNLQEQNRVFELARTHPTIIKRNE
PIMDAYDDFMNNKTGETLVKALIQSSILEGINFYSGFAYFYNLVRQNKMTGTGKIISFINRDELAHSKFISEVIRAILGE
NPELQTEELVEYTHDAFRHAVELETEWSEEVLQGIEGIDVEEMVDYVKYRANKMLGMLGIPELYPGHSDNTMTWIKAYAD
NFTETKTDFFEMRNSSYKKTNMDNGFDDL

Sequences:

>Translated_349_residues
MANQKEQLTRIKILEPLFPNRSTSIINGETSGILNWNDIPYPSFYRAYKELSTNYWIPDEVDMKSDAKQYPALSEQEKYA
FDAIIGLLATLDSPQTRFIYNIAEYITDPAVHANAAIIAQQEVIHNESYSYVLASITNLQEQNRVFELARTHPTIIKRNE
PIMDAYDDFMNNKTGETLVKALIQSSILEGINFYSGFAYFYNLVRQNKMTGTGKIISFINRDELAHSKFISEVIRAILGE
NPELQTEELVEYTHDAFRHAVELETEWSEEVLQGIEGIDVEEMVDYVKYRANKMLGMLGIPELYPGHSDNTMTWIKAYAD
NFTETKTDFFEMRNSSYKKTNMDNGFDDL
>Mature_348_residues
ANQKEQLTRIKILEPLFPNRSTSIINGETSGILNWNDIPYPSFYRAYKELSTNYWIPDEVDMKSDAKQYPALSEQEKYAF
DAIIGLLATLDSPQTRFIYNIAEYITDPAVHANAAIIAQQEVIHNESYSYVLASITNLQEQNRVFELARTHPTIIKRNEP
IMDAYDDFMNNKTGETLVKALIQSSILEGINFYSGFAYFYNLVRQNKMTGTGKIISFINRDELAHSKFISEVIRAILGEN
PELQTEELVEYTHDAFRHAVELETEWSEEVLQGIEGIDVEEMVDYVKYRANKMLGMLGIPELYPGHSDNTMTWIKAYADN
FTETKTDFFEMRNSSYKKTNMDNGFDDL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=304, Percent_Identity=23.0263157894737, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI289177074, Length=304, Percent_Identity=23.0263157894737, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI4557845, Length=307, Percent_Identity=21.8241042345277, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI260064013, Length=307, Percent_Identity=21.8241042345277, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI289177076, Length=284, Percent_Identity=22.887323943662, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788567, Length=355, Percent_Identity=26.1971830985915, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1789031, Length=307, Percent_Identity=23.7785016286645, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17551968, Length=307, Percent_Identity=23.4527687296417, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322434, Length=308, Percent_Identity=24.025974025974, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI18079279, Length=316, Percent_Identity=21.2025316455696, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 40204; Mature: 40072

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANQKEQLTRIKILEPLFPNRSTSIINGETSGILNWNDIPYPSFYRAYKELSTNYWIPDE
CCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCC
VDMKSDAKQYPALSEQEKYAFDAIIGLLATLDSPQTRFIYNIAEYITDPAVHANAAIIAQ
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHCCHHHHHH
QEVIHNESYSYVLASITNLQEQNRVFELARTHPTIIKRNEPIMDAYDDFMNNKTGETLVK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCHHHHHH
ALIQSSILEGINFYSGFAYFYNLVRQNKMTGTGKIISFINRDELAHSKFISEVIRAILGE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPELQTEELVEYTHDAFRHAVELETEWSEEVLQGIEGIDVEEMVDYVKYRANKMLGMLGI
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
PELYPGHSDNTMTWIKAYADNFTETKTDFFEMRNSSYKKTNMDNGFDDL
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ANQKEQLTRIKILEPLFPNRSTSIINGETSGILNWNDIPYPSFYRAYKELSTNYWIPDE
CCHHHHHHHHHHHHCCCCCCCCEEECCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCC
VDMKSDAKQYPALSEQEKYAFDAIIGLLATLDSPQTRFIYNIAEYITDPAVHANAAIIAQ
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHCCHHHHHH
QEVIHNESYSYVLASITNLQEQNRVFELARTHPTIIKRNEPIMDAYDDFMNNKTGETLVK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCHHHHHH
ALIQSSILEGINFYSGFAYFYNLVRQNKMTGTGKIISFINRDELAHSKFISEVIRAILGE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPELQTEELVEYTHDAFRHAVELETEWSEEVLQGIEGIDVEEMVDYVKYRANKMLGMLGI
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
PELYPGHSDNTMTWIKAYADNFTETKTDFFEMRNSSYKKTNMDNGFDDL
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]