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The map label for this gene is nfrA2 [H]

Identifier: 226224717

GI number: 226224717

Start: 2191190

End: 2191921

Strand: Reverse

Name: nfrA2 [H]

Synonym: Lm4b_02133

Alternate gene names: 226224717

Gene position: 2191921-2191190 (Counterclockwise)

Preceding gene: 226224722

Following gene: 226224716

Centisome position: 75.25

GC content: 40.03

Gene sequence:

>732_bases
ATGAATGACGTATTAACTTTGCTAAGAAATCATCGCTCTTTTCGTAAATATAAAAAAGGGGTTGAGATTCCGGAAGAAAA
TCTAGATGCGATTATCCGAGCAGCTCAAGCAGCCCCGTCGTGGATTAATGGTCAACACTATTCGATTATTGCGATTAAAG
ATCAAGAACGTAAAAATAAAATGGCCGAACTTTGCGGAAATCAACCTTACATAGCAGAATGTTCCGTTTTTCTTTGTTTT
GTGGCAGATTTTCACCGCGTAAAAGTAGCGAGTGACATGCACGGCAAAAGTTTCCAAATTGCCGGGGAAGAAGATTTACT
AATGGTTGCGGCAACAGACATCGGACTTTGTATGCAAAATGCTTTAACAGCTGCTGAGTCGCTTGATTACGGGACGATTT
GTATTGGTGGTTTGCGCAGAAATATTACGGCAACCGCTGAATTTCTAAATTTACCAGAATTCGTCATGCCCGTTGTGGGA
CTTTGTATTGGCGTTCCGGATGTCGAAGCACCTGTAAAACCACGTTTGCCAAAAGAAGCAGTTTATTTTGAAGAGACGTA
CCAAACTGATGTCATGCCTTTACTAGAAGCATATGACCAAGAGATTATTCCGTTTTCAGAGCGAGAAGGCTTTGTTTCTT
ATACCGAACGGTTAGCCAAATTTTATGACAGACCTTATTATCCAAATTTAACAGAACAAATAAAAGAAAGAGGCTTTTTA
GGCGGGAAATAA

Upstream 100 bases:

>100_bases
AAGTGTAGAAAATATCTGGCAGAAATACAAGAGAAAACATTAACAATTGCCTAAAAACAAGCTATACTTTAACTAAATGA
TAAATCTAGGAGGCATAAAT

Downstream 100 bases:

>100_bases
CTAGCATCTTCGCCATTATTAGTATATACTTTTAATTGACAAAACCCAGCAAAAACGCTGGAATGGAGGTAATTTTATAA
TGACAGAAGCATTATTTTTA

Product: FMN-containing NADPH-linked nitro/flavin reductase

Products: NA

Alternate protein names: NAD(P)H-dependent FMN reductase; NAD(P)H-dependent nitroreductase; NAD(P)H-dependent oxidoreductase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MNDVLTLLRNHRSFRKYKKGVEIPEENLDAIIRAAQAAPSWINGQHYSIIAIKDQERKNKMAELCGNQPYIAECSVFLCF
VADFHRVKVASDMHGKSFQIAGEEDLLMVAATDIGLCMQNALTAAESLDYGTICIGGLRRNITATAEFLNLPEFVMPVVG
LCIGVPDVEAPVKPRLPKEAVYFEETYQTDVMPLLEAYDQEIIPFSEREGFVSYTERLAKFYDRPYYPNLTEQIKERGFL
GGK

Sequences:

>Translated_243_residues
MNDVLTLLRNHRSFRKYKKGVEIPEENLDAIIRAAQAAPSWINGQHYSIIAIKDQERKNKMAELCGNQPYIAECSVFLCF
VADFHRVKVASDMHGKSFQIAGEEDLLMVAATDIGLCMQNALTAAESLDYGTICIGGLRRNITATAEFLNLPEFVMPVVG
LCIGVPDVEAPVKPRLPKEAVYFEETYQTDVMPLLEAYDQEIIPFSEREGFVSYTERLAKFYDRPYYPNLTEQIKERGFL
GGK
>Mature_243_residues
MNDVLTLLRNHRSFRKYKKGVEIPEENLDAIIRAAQAAPSWINGQHYSIIAIKDQERKNKMAELCGNQPYIAECSVFLCF
VADFHRVKVASDMHGKSFQIAGEEDLLMVAATDIGLCMQNALTAAESLDYGTICIGGLRRNITATAEFLNLPEFVMPVVG
LCIGVPDVEAPVKPRLPKEAVYFEETYQTDVMPLLEAYDQEIIPFSEREGFVSYTERLAKFYDRPYYPNLTEQIKERGFL
GGK

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones, chromates and azo dyes. It could supply the reduced form of FMN to luciferase-like protein and contribute to the degradation of aromatic compounds [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=234, Percent_Identity=32.9059829059829, Blast_Score=118, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 27427; Mature: 27427

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVLTLLRNHRSFRKYKKGVEIPEENLDAIIRAAQAAPSWINGQHYSIIAIKDQERKNK
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHH
MAELCGNQPYIAECSVFLCFVADFHRVKVASDMHGKSFQIAGEEDLLMVAATDIGLCMQN
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECHHHHHHHH
ALTAAESLDYGTICIGGLRRNITATAEFLNLPEFVMPVVGLCIGVPDVEAPVKPRLPKEA
HHHHHHCCCCCCEEECCHHHCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VYFEETYQTDVMPLLEAYDQEIIPFSEREGFVSYTERLAKFYDRPYYPNLTEQIKERGFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
GGK
CCC
>Mature Secondary Structure
MNDVLTLLRNHRSFRKYKKGVEIPEENLDAIIRAAQAAPSWINGQHYSIIAIKDQERKNK
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHH
MAELCGNQPYIAECSVFLCFVADFHRVKVASDMHGKSFQIAGEEDLLMVAATDIGLCMQN
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECHHHHHHHH
ALTAAESLDYGTICIGGLRRNITATAEFLNLPEFVMPVVGLCIGVPDVEAPVKPRLPKEA
HHHHHHCCCCCCEEECCHHHCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VYFEETYQTDVMPLLEAYDQEIIPFSEREGFVSYTERLAKFYDRPYYPNLTEQIKERGFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
GGK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]