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The map label for this gene is murD

Identifier: 226224642

GI number: 226224642

Start: 2116937

End: 2118304

Strand: Reverse

Name: murD

Synonym: Lm4b_02057

Alternate gene names: 226224642

Gene position: 2118304-2116937 (Counterclockwise)

Preceding gene: 226224643

Following gene: 226224641

Centisome position: 72.73

GC content: 38.89

Gene sequence:

>1368_bases
ATGAAAAAAATTGAAATGTACCATCACAAAAAAGTACTTGTCTTGGGTCTTGCAAGAAGTGGTGTTAGCGCGGCAACAAT
TATGCACAAACTGGGAGCATTTGTAACTGTTAATGATCAAAAACCTTTTAGCGAGAATCCAGAAGCACAAGGTTTACTTG
AGCAAGGTATTAAAGTTATTTGCGGTTCGCACCCAATTGAACTTTTAGATGAAGGATTTGAACTTGTCATAAAAAATCCT
GGAATTCCGTACAACAACCCAATGATTGAAAAAGCGTTAAAACTCAAAATTCCAGTCATTACGGAAGTCGAATTAGCTTA
CCAAATATCTGAAGCACCAATTGTTGGTATTACAGGAACAAATGGTAAAACGACAACAACCACAATCATCCACCATATGT
TAAATGCGCACAAAGAAAATAGTTCTTTACTCGCAGGAAACATCGGTTTTCCGGCATCCGCAGTTGCAGAAAATGCGACC
AGTGATCAATACATTTCGATGGAGCTATCTTCGTTCCAATTAATGGGCGTAGAAACATTTAAACCGCATATTTCCGTCAT
CACGAATATTTATGAAGCGCATTTAGATTACCATACAGACCGTAGTGAATACGTTCAAGCAAAATGGCATATCCAAAAAA
ATCAAACAGCAGATGATTTCCTAGTAATTAATTGGGATCAAGAAGAGCTTAAAAACCTAACAAAACAAACAAAAGCACAA
GTTATTCCGTTTTCAACCACACAACGCCTTGGTCAAGGAAGCTACGTGCAAAATGGCAATATTATGTTCAACGATGAAGT
GATTGGCGCACGTGACAATATTTTACTTCCAGGAGAGCATAATTTAGAAAATGTGCTTGCTTCCGTAGCAGTAGCAAAAA
CGTTAGGTGTTACAAATGAAGAAATCATGCACGTATTAGAAACGTTTAAAGGTGTAGAGCACCGGACACAATTTGTTGTA
GAATGGCAAGGTCGTAAATTCTATAACGATTCAAAAGCAACGAATATTCTAGCAACTCAAAGTGCATTAAAAGGGTTTAA
AAATCCAGTCGTGTTACTGGCTGGTGGGTTGGACCGTGGTAATTCATTTGACGAATTACTACCATTTTTCAAAAATGTAA
AAGCGTTAATTGTCTTTGGCGAAACAGCAGATAAAATTGGCCGCGTTGGTAAAATCGCTGGCATTGACGTACATTACGTG
GATAATGTCGAGGCAGCTGTTCCAGTTGCGTACCGCGAATCAGCACCAGGAGATATTATCTTACTTTCACCAGCATGTGC
GAGTTGGGATCAATACCGAACATTTGAAGTTCGCGGGAATGCCTATATGGACGCAATCGGCGAGCTAATAGAAGAGGTGG
AAAAATGA

Upstream 100 bases:

>100_bases
TAGCTGTTTTTTTACAGAATCTGGTTGGCGCTTTCCGTCGTTTTTGTGAAAATCATAAATTCGACCGCCAATTTTGATAT
AAATGGGGGAACCAAAGGAA

Downstream 100 bases:

>100_bases
AAGTAGCAATAAGTGGTGGCGGTACTGGTGGACACGTTTATCCAGCGCTTGCTCTCATCAGAGAATTAAAAAAAATTCAT
CCTGAAGCGGAATTTTTATA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKKIEMYHHKKVLVLGLARSGVSAATIMHKLGAFVTVNDQKPFSENPEAQGLLEQGIKVICGSHPIELLDEGFELVIKNP
GIPYNNPMIEKALKLKIPVITEVELAYQISEAPIVGITGTNGKTTTTTIIHHMLNAHKENSSLLAGNIGFPASAVAENAT
SDQYISMELSSFQLMGVETFKPHISVITNIYEAHLDYHTDRSEYVQAKWHIQKNQTADDFLVINWDQEELKNLTKQTKAQ
VIPFSTTQRLGQGSYVQNGNIMFNDEVIGARDNILLPGEHNLENVLASVAVAKTLGVTNEEIMHVLETFKGVEHRTQFVV
EWQGRKFYNDSKATNILATQSALKGFKNPVVLLAGGLDRGNSFDELLPFFKNVKALIVFGETADKIGRVGKIAGIDVHYV
DNVEAAVPVAYRESAPGDIILLSPACASWDQYRTFEVRGNAYMDAIGELIEEVEK

Sequences:

>Translated_455_residues
MKKIEMYHHKKVLVLGLARSGVSAATIMHKLGAFVTVNDQKPFSENPEAQGLLEQGIKVICGSHPIELLDEGFELVIKNP
GIPYNNPMIEKALKLKIPVITEVELAYQISEAPIVGITGTNGKTTTTTIIHHMLNAHKENSSLLAGNIGFPASAVAENAT
SDQYISMELSSFQLMGVETFKPHISVITNIYEAHLDYHTDRSEYVQAKWHIQKNQTADDFLVINWDQEELKNLTKQTKAQ
VIPFSTTQRLGQGSYVQNGNIMFNDEVIGARDNILLPGEHNLENVLASVAVAKTLGVTNEEIMHVLETFKGVEHRTQFVV
EWQGRKFYNDSKATNILATQSALKGFKNPVVLLAGGLDRGNSFDELLPFFKNVKALIVFGETADKIGRVGKIAGIDVHYV
DNVEAAVPVAYRESAPGDIILLSPACASWDQYRTFEVRGNAYMDAIGELIEEVEK
>Mature_455_residues
MKKIEMYHHKKVLVLGLARSGVSAATIMHKLGAFVTVNDQKPFSENPEAQGLLEQGIKVICGSHPIELLDEGFELVIKNP
GIPYNNPMIEKALKLKIPVITEVELAYQISEAPIVGITGTNGKTTTTTIIHHMLNAHKENSSLLAGNIGFPASAVAENAT
SDQYISMELSSFQLMGVETFKPHISVITNIYEAHLDYHTDRSEYVQAKWHIQKNQTADDFLVINWDQEELKNLTKQTKAQ
VIPFSTTQRLGQGSYVQNGNIMFNDEVIGARDNILLPGEHNLENVLASVAVAKTLGVTNEEIMHVLETFKGVEHRTQFVV
EWQGRKFYNDSKATNILATQSALKGFKNPVVLLAGGLDRGNSFDELLPFFKNVKALIVFGETADKIGRVGKIAGIDVHYV
DNVEAAVPVAYRESAPGDIILLSPACASWDQYRTFEVRGNAYMDAIGELIEEVEK

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=454, Percent_Identity=30.6167400881057, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 50287; Mature: 50287

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIEMYHHKKVLVLGLARSGVSAATIMHKLGAFVTVNDQKPFSENPEAQGLLEQGIKVI
CCCCCHHHCCEEEEEEECCCCCHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHCHHEE
CGSHPIELLDEGFELVIKNPGIPYNNPMIEKALKLKIPVITEVELAYQISEAPIVGITGT
CCCCCHHHHCCCCEEEEECCCCCCCCHHHHHHHHEECCEEEEEEEEEEECCCCEEEEECC
NGKTTTTTIIHHMLNAHKENSSLLAGNIGFPASAVAENATSDQYISMELSSFQLMGVETF
CCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHCCCCCCCEEEEEECCEEEEEEHHC
KPHISVITNIYEAHLDYHTDRSEYVQAKWHIQKNQTADDFLVINWDQEELKNLTKQTKAQ
CCHHHHHHHHHHHHCCCCCCHHHHEEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHCE
VIPFSTTQRLGQGSYVQNGNIMFNDEVIGARDNILLPGEHNLENVLASVAVAKTLGVTNE
EECCHHHHHCCCCCEEECCCEEECCCEECCCCCEEECCCCCHHHHHHHHHHHHHHCCCHH
EIMHVLETFKGVEHRTQFVVEWQGRKFYNDSKATNILATQSALKGFKNPVVLLAGGLDRG
HHHHHHHHHHCCCCCEEEEEEECCCEEECCCCHHEEEEHHHHHHHCCCCEEEEECCCCCC
NSFDELLPFFKNVKALIVFGETADKIGRVGKIAGIDVHYVDNVEAAVPVAYRESAPGDII
CCHHHHHHHHCCCEEEEEECCCHHHHCCCCEEECEEEEEECCCCHHCCEEECCCCCCCEE
LLSPACASWDQYRTFEVRGNAYMDAIGELIEEVEK
EECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIEMYHHKKVLVLGLARSGVSAATIMHKLGAFVTVNDQKPFSENPEAQGLLEQGIKVI
CCCCCHHHCCEEEEEEECCCCCHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHCHHEE
CGSHPIELLDEGFELVIKNPGIPYNNPMIEKALKLKIPVITEVELAYQISEAPIVGITGT
CCCCCHHHHCCCCEEEEECCCCCCCCHHHHHHHHEECCEEEEEEEEEEECCCCEEEEECC
NGKTTTTTIIHHMLNAHKENSSLLAGNIGFPASAVAENATSDQYISMELSSFQLMGVETF
CCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHCCCCCCCEEEEEECCEEEEEEHHC
KPHISVITNIYEAHLDYHTDRSEYVQAKWHIQKNQTADDFLVINWDQEELKNLTKQTKAQ
CCHHHHHHHHHHHHCCCCCCHHHHEEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHCE
VIPFSTTQRLGQGSYVQNGNIMFNDEVIGARDNILLPGEHNLENVLASVAVAKTLGVTNE
EECCHHHHHCCCCCEEECCCEEECCCEECCCCCEEECCCCCHHHHHHHHHHHHHHCCCHH
EIMHVLETFKGVEHRTQFVVEWQGRKFYNDSKATNILATQSALKGFKNPVVLLAGGLDRG
HHHHHHHHHHCCCCCEEEEEEECCCEEECCCCHHEEEEHHHHHHHCCCCEEEEECCCCCC
NSFDELLPFFKNVKALIVFGETADKIGRVGKIAGIDVHYVDNVEAAVPVAYRESAPGDII
CCHHHHHHHHCCCEEEEEECCCHHHHCCCCEEECEEEEEECCCCHHCCEEECCCCCCCEE
LLSPACASWDQYRTFEVRGNAYMDAIGELIEEVEK
EECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]