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The map label for this gene is dapF

Identifier: 226224615

GI number: 226224615

Start: 2084811

End: 2085800

Strand: Reverse

Name: dapF

Synonym: Lm4b_02030

Alternate gene names: 226224615

Gene position: 2085800-2084811 (Counterclockwise)

Preceding gene: 226224616

Following gene: 226224614

Centisome position: 71.61

GC content: 38.48

Gene sequence:

>990_bases
ATGGAAACAATTCACTTTACAAAAGTACATGGTTCGCAAAACGATTTTTTCTTAGTTGATGAAGAGGAAAATCACATCAC
AGAATGGTCGGATGAAAAACGTGCTAATTTTGCGATAAAGCTTTGCGATAGAAAGCACTCGCTTGGCGGGGCAGACGGAA
TTTTATATGTCACAAAAAGCAGTGAAGTTGGACCAATTGGCCAAATGCGTGTCGTTAATTCAGATGGCTCAATTGCTTCG
ATGTGCGGGAATGGCTTAAGAACGGTAGCTCGCTATTTACTTGAGAAACATGCTTTGACAGATGCAAAAGTAGAAACTAT
GAAAGCGATACTTGATGTCAAAAAAGCAACTTCGCTTGGTTTTGATATTCCAACCTATCAAGTGGAAATTTCTCCTGTTA
AATTTGCAGCAGAAACATTACCGATGCATGTAGGCGTGGAAAAATTATTTAATCAAGTTATCCCAGAACTTGATGCAGAA
CTCGCTTTTTCTGCTGTTTCTGTTCCTAATCCGCATTTGATTACATTTGTGGACCAAGCTGTGCTAGATTCCAATAAACA
AGAAAAATTAGCAAGTTATTTAAATAGCGAAAATCCGTATTTCCCAGATGGCGTGAATGTAAGCTTTGTGAAACGTTTAA
GCGATGATGCTATTTATGTGCGTACTTTTGAACGCGGCGTCGGTTTTACGAATGCATGTGGAACAGCGATGTCGGCTTGT
AGTTTGATTAAAAAAATGTTAGATAATGATATATTGGAAACGCCGCTAAATGTTTATAATGATGGCGGAAGAGTGCAAGT
AACTGCGAAAAAAGATGCTGCTGGTGAAATTTCGCTACAACTGATTGGCAATGCCACTTTTGTTAGCAAAGGTTCCGTTC
GTTATGAAAATGATGTCGTTACAGAGCTTACAAATGAAGCGACAGATGAACAAGGTCAGTATCAGGCGCTTGTAAAAGAA
GTAAAAGAATTTCTAAAAACAACCGAATGA

Upstream 100 bases:

>100_bases
GGAAAATCCTACTCATTTCAAAAGGAGGTTCCCCAAGCTTTTTTAATTTAAGATGTGGTATTATAGACGTAATTCCAAAT
AGAGGAGGTTGCTTGACGTA

Downstream 100 bases:

>100_bases
AAAGGAGTTAACCATGAATACAAATCGTAAAAAAGCAACACCGTTATTCGTTATTGGCGGATTTTGCCTTATTTTATTTA
TTACTATCGCTTCTGCTATC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
METIHFTKVHGSQNDFFLVDEEENHITEWSDEKRANFAIKLCDRKHSLGGADGILYVTKSSEVGPIGQMRVVNSDGSIAS
MCGNGLRTVARYLLEKHALTDAKVETMKAILDVKKATSLGFDIPTYQVEISPVKFAAETLPMHVGVEKLFNQVIPELDAE
LAFSAVSVPNPHLITFVDQAVLDSNKQEKLASYLNSENPYFPDGVNVSFVKRLSDDAIYVRTFERGVGFTNACGTAMSAC
SLIKKMLDNDILETPLNVYNDGGRVQVTAKKDAAGEISLQLIGNATFVSKGSVRYENDVVTELTNEATDEQGQYQALVKE
VKEFLKTTE

Sequences:

>Translated_329_residues
METIHFTKVHGSQNDFFLVDEEENHITEWSDEKRANFAIKLCDRKHSLGGADGILYVTKSSEVGPIGQMRVVNSDGSIAS
MCGNGLRTVARYLLEKHALTDAKVETMKAILDVKKATSLGFDIPTYQVEISPVKFAAETLPMHVGVEKLFNQVIPELDAE
LAFSAVSVPNPHLITFVDQAVLDSNKQEKLASYLNSENPYFPDGVNVSFVKRLSDDAIYVRTFERGVGFTNACGTAMSAC
SLIKKMLDNDILETPLNVYNDGGRVQVTAKKDAAGEISLQLIGNATFVSKGSVRYENDVVTELTNEATDEQGQYQALVKE
VKEFLKTTE
>Mature_329_residues
METIHFTKVHGSQNDFFLVDEEENHITEWSDEKRANFAIKLCDRKHSLGGADGILYVTKSSEVGPIGQMRVVNSDGSIAS
MCGNGLRTVARYLLEKHALTDAKVETMKAILDVKKATSLGFDIPTYQVEISPVKFAAETLPMHVGVEKLFNQVIPELDAE
LAFSAVSVPNPHLITFVDQAVLDSNKQEKLASYLNSENPYFPDGVNVSFVKRLSDDAIYVRTFERGVGFTNACGTAMSAC
SLIKKMLDNDILETPLNVYNDGGRVQVTAKKDAAGEISLQLIGNATFVSKGSVRYENDVVTELTNEATDEQGQYQALVKE
VKEFLKTTE

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=295, Percent_Identity=28.4745762711864, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 36227; Mature: 36227

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIHFTKVHGSQNDFFLVDEEENHITEWSDEKRANFAIKLCDRKHSLGGADGILYVTKS
CCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECC
SEVGPIGQMRVVNSDGSIASMCGNGLRTVARYLLEKHALTDAKVETMKAILDVKKATSLG
CCCCCCCCEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
FDIPTYQVEISPVKFAAETLPMHVGVEKLFNQVIPELDAELAFSAVSVPNPHLITFVDQA
CCCCEEEEEECHHHHHHHHCCHHHCHHHHHHHHHHHHCHHHHHHEECCCCCCEEHHHHHH
VLDSNKQEKLASYLNSENPYFPDGVNVSFVKRLSDDAIYVRTFERGVGFTNACGTAMSAC
HHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEEEEEHHCCCCCHHHHHHHHHH
SLIKKMLDNDILETPLNVYNDGGRVQVTAKKDAAGEISLQLIGNATFVSKGSVRYENDVV
HHHHHHHCCHHHHCCHHHCCCCCEEEEEECCCCCCEEEEEEECCEEEEECCCCEECCHHH
TELTNEATDEQGQYQALVKEVKEFLKTTE
HHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METIHFTKVHGSQNDFFLVDEEENHITEWSDEKRANFAIKLCDRKHSLGGADGILYVTKS
CCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECC
SEVGPIGQMRVVNSDGSIASMCGNGLRTVARYLLEKHALTDAKVETMKAILDVKKATSLG
CCCCCCCCEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
FDIPTYQVEISPVKFAAETLPMHVGVEKLFNQVIPELDAELAFSAVSVPNPHLITFVDQA
CCCCEEEEEECHHHHHHHHCCHHHCHHHHHHHHHHHHCHHHHHHEECCCCCCEEHHHHHH
VLDSNKQEKLASYLNSENPYFPDGVNVSFVKRLSDDAIYVRTFERGVGFTNACGTAMSAC
HHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEEEEEHHCCCCCHHHHHHHHHH
SLIKKMLDNDILETPLNVYNDGGRVQVTAKKDAAGEISLQLIGNATFVSKGSVRYENDVV
HHHHHHHCCHHHHCCHHHCCCCCEEEEEECCCCCCEEEEEEECCEEEEECCCCEECCHHH
TELTNEATDEQGQYQALVKEVKEFLKTTE
HHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]