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The map label for this gene is ansA [H]

Identifier: 226224544

GI number: 226224544

Start: 2002639

End: 2003601

Strand: Reverse

Name: ansA [H]

Synonym: Lm4b_01957

Alternate gene names: 226224544

Gene position: 2003601-2002639 (Counterclockwise)

Preceding gene: 226224545

Following gene: 226224543

Centisome position: 68.79

GC content: 37.69

Gene sequence:

>963_bases
ATGGCAAAAGTAGCACTAATTACAACAGGCGGTACAATTGCAAGTAAGAAAACAGCATCAGGAAAACTTGCATCAGGAGA
ATTATCTGGAGAAGAATTAGCTGCATTATGCCAATTACCAACAGAAATTCAAATTGATATCTATTCTACTTTCCAATTAC
CATCTATGCACATTACGCTACCTGATTTAATCAGTCTGAAGCAACTTATCGAATCTATCTTCATGGATGAAACATATGAT
GGAATAGTAGTGACGCACGGAACAGATTCATTAGAAGAAACAGCCTATTTTCTTGATCTTGCAGTAACCGATGCGCGACC
AATCGTAGTAACTGGATCTCAGCGAGCACCTGAGGAGCCAGGGACAGATGCCTATGTTAATATTCGTCATGCAATTTATA
CAGCGTGTGAAATAAATTTACGTCAAGCTGGGACAGTCGTTGTTTTCAATGAACGAATTTTTGCAGCGAGATATGTAAAA
AAAGTTCATGCATCCAACATACAAGGTTTTAGTGCTTTTGGGTTTGGCTATCTGGGTATTATTGATAATGACCAAGTTTT
TCTGTATCAAAAACCACTGGAGCATGAGTGTTTTGATATTCGCCTTGATTTACCAGAAGTGGTTGTGATTAAATGTTATA
TTGGTGCAGATGGTTTGTTTATTGATGCAGCGATAGATAGTGGCGTATCAGGAATCGTTTTAGAAGGTGTTGGACGAGGG
CAAGTGGCGCCAAAAATGATGCCAGCTATTATCAGAGCATTAGATGCGGGAATTCCAGTTGTCATAACAACAAGTGCGGA
AGAAGGAAATGTATATACTACATATGATTATGAAGGTAGCACGTTTGACTTGTATAATCGTGGCGTTATTTTAGGAAAAG
ATTACGATAGCAAAAAAGCACGGATGAAATTAATGGTACTTTTAGCATCACAAGAAGAAATTAATCAAACCAATTTCAGA
TAA

Upstream 100 bases:

>100_bases
TTCTAACAATTCCACAGTAATAAACAACACTGAATGGGTCTGGGAAACGCTTCCGAGACTCATTTGTTTGTTTAAAAGGA
AAATGTAGGGGGGAATAACC

Downstream 100 bases:

>100_bases
AAGGAGTTTTATTTTATGACTAAAAAGATATGTATCGCGATTGATGGACCGGCTGCGGCTGGGAAAAGCACGGTGGCGAA
AATCGTTGCAAAAAAATTGC

Product: asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MAKVALITTGGTIASKKTASGKLASGELSGEELAALCQLPTEIQIDIYSTFQLPSMHITLPDLISLKQLIESIFMDETYD
GIVVTHGTDSLEETAYFLDLAVTDARPIVVTGSQRAPEEPGTDAYVNIRHAIYTACEINLRQAGTVVVFNERIFAARYVK
KVHASNIQGFSAFGFGYLGIIDNDQVFLYQKPLEHECFDIRLDLPEVVVIKCYIGADGLFIDAAIDSGVSGIVLEGVGRG
QVAPKMMPAIIRALDAGIPVVITTSAEEGNVYTTYDYEGSTFDLYNRGVILGKDYDSKKARMKLMVLLASQEEINQTNFR

Sequences:

>Translated_320_residues
MAKVALITTGGTIASKKTASGKLASGELSGEELAALCQLPTEIQIDIYSTFQLPSMHITLPDLISLKQLIESIFMDETYD
GIVVTHGTDSLEETAYFLDLAVTDARPIVVTGSQRAPEEPGTDAYVNIRHAIYTACEINLRQAGTVVVFNERIFAARYVK
KVHASNIQGFSAFGFGYLGIIDNDQVFLYQKPLEHECFDIRLDLPEVVVIKCYIGADGLFIDAAIDSGVSGIVLEGVGRG
QVAPKMMPAIIRALDAGIPVVITTSAEEGNVYTTYDYEGSTFDLYNRGVILGKDYDSKKARMKLMVLLASQEEINQTNFR
>Mature_319_residues
AKVALITTGGTIASKKTASGKLASGELSGEELAALCQLPTEIQIDIYSTFQLPSMHITLPDLISLKQLIESIFMDETYDG
IVVTHGTDSLEETAYFLDLAVTDARPIVVTGSQRAPEEPGTDAYVNIRHAIYTACEINLRQAGTVVVFNERIFAARYVKK
VHASNIQGFSAFGFGYLGIIDNDQVFLYQKPLEHECFDIRLDLPEVVVIKCYIGADGLFIDAAIDSGVSGIVLEGVGRGQ
VAPKMMPAIIRALDAGIPVVITTSAEEGNVYTTYDYEGSTFDLYNRGVILGKDYDSKKARMKLMVLLASQEEINQTNFR

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=243, Percent_Identity=31.2757201646091, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788065, Length=317, Percent_Identity=27.7602523659306, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323189, Length=286, Percent_Identity=30.4195804195804, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323187, Length=286, Percent_Identity=30.4195804195804, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323186, Length=286, Percent_Identity=30.4195804195804, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323184, Length=286, Percent_Identity=30.4195804195804, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320527, Length=333, Percent_Identity=27.3273273273273, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 34925; Mature: 34794

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVALITTGGTIASKKTASGKLASGELSGEELAALCQLPTEIQIDIYSTFQLPSMHITL
CCEEEEEEECCCEECCCCCCCCEECCCCCHHHHHHHHCCCCEEEEEEEEEEECCEEEEEC
PDLISLKQLIESIFMDETYDGIVVTHGTDSLEETAYFLDLAVTDARPIVVTGSQRAPEEP
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHEEEEEEEEECCCEEEEECCCCCCCCC
GTDAYVNIRHAIYTACEINLRQAGTVVVFNERIFAARYVKKVHASNIQGFSAFGFGYLGI
CCCCEEEEEEHEEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHCCCCCEEECCCEEEEE
IDNDQVFLYQKPLEHECFDIRLDLPEVVVIKCYIGADGLFIDAAIDSGVSGIVLEGVGRG
EECCEEEEEECCCCCCEEEEEECCCCEEEEEEEECCCCEEEEEECCCCCCEEEEECCCCC
QVAPKMMPAIIRALDAGIPVVITTSAEEGNVYTTYDYEGSTFDLYNRGVILGKDYDSKKA
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCEEEEECCCEEEECCCCCHHH
RMKLMVLLASQEEINQTNFR
EEEEEEEEECCHHHHCCCCC
>Mature Secondary Structure 
AKVALITTGGTIASKKTASGKLASGELSGEELAALCQLPTEIQIDIYSTFQLPSMHITL
CEEEEEEECCCEECCCCCCCCEECCCCCHHHHHHHHCCCCEEEEEEEEEEECCEEEEEC
PDLISLKQLIESIFMDETYDGIVVTHGTDSLEETAYFLDLAVTDARPIVVTGSQRAPEEP
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHEEEEEEEEECCCEEEEECCCCCCCCC
GTDAYVNIRHAIYTACEINLRQAGTVVVFNERIFAARYVKKVHASNIQGFSAFGFGYLGI
CCCCEEEEEEHEEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHCCCCCEEECCCEEEEE
IDNDQVFLYQKPLEHECFDIRLDLPEVVVIKCYIGADGLFIDAAIDSGVSGIVLEGVGRG
EECCEEEEEECCCCCCEEEEEECCCCEEEEEEEECCCCEEEEEECCCCCCEEEEECCCCC
QVAPKMMPAIIRALDAGIPVVITTSAEEGNVYTTYDYEGSTFDLYNRGVILGKDYDSKKA
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCEEEEECCCEEEECCCCCHHH
RMKLMVLLASQEEINQTNFR
EEEEEEEEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]