Click here to switch to the map view.

The map label for this gene is engA

Identifier: 226224541

GI number: 226224541

Start: 1998991

End: 2000301

Strand: Reverse

Name: engA

Synonym: Lm4b_01954

Alternate gene names: 226224541

Gene position: 2000301-1998991 (Counterclockwise)

Preceding gene: 226224542

Following gene: 226224540

Centisome position: 68.68

GC content: 38.9

Gene sequence:

>1311_bases
ATGGCAAAACCAGTTGTAGCGATTGTCGGACGTCCAAACGTTGGCAAATCGACTATTTTTAACAGAATCGTTGGTGAACG
TGTTTCCATAGTGGAAGATGTTCCCGGTGTGACACGTGACCGCATATATAATTCAGCGGAATGGCTTGGAAAAGAATTTA
ACATTATTGATACAGGTGGTATTGATCTTTCCGATGAACCATTCTTAGAGCAAATTCGCGCACAAGCGGAAATCGCAATT
GATGAAGCAGACGTAATTATTTTTATTACCAATGGTCGTGAAGGGGTTACCGATGCAGACGAACAAGTAGCAAAAATTCT
TTACCGGTCTAATAAACCAATTGTTTTAGCGATTAATAAAGTAGATAACCCAGAAATGCGAGATCAAATTTATGACTTTT
ATTCTCTTGGGTTTGGTGAACCGTATCCAATTTCTGGTTCACATGGGCTAGGGCTTGGCGACATGCTTGATGCTGTTCGG
GCTCATTTTCCAAAAGAAGAAGAGGAAGAATATCCAGACGATACAGTGAAATTTAGTTTGATTGGTCGACCAAATGTTGG
TAAATCTTCTATTTTAAATGCACTGCTTGGAGAAGATCGTGTTATTGTTTCTGATATTGCGGGTACAACTCGTGATGCAA
TTGATACAACTTATACTTTCGATGGCCAAGATTATGTCATGATTGATACAGCCGGAATGAGAAAACGTGGGAAAGTGTAT
GAAAGCACAGAGAAATATAGTGTTTTACGTGCAATGAGAGCAATTGAACGCTCAGACGTTGTTCTTGTGGTTATCAACGC
AGAAGAAGGTATTCGTGAGCAAGATAAACGAATTGCTGGATATGCGCATGATGCCGGACGTGCGATTATTATTGTAGTAA
ACAAATGGGATGCAATTAACAAAGATGAAAAAACAATTAACGTATGGACGGAAGATATTCGGGAGCAATTCCAATTCTTA
AGCTATGCACCAATTGTCTTTGTATCTGCGAAAACAAAACAACGCTTAAATAACCTATTCCCACTTATTAATCAAGTAAG
CGATAACCATTCATTACGTGTACAATCAAGTATGCTGAATGATGTTATTAGTGATGCCGTTGCAATGAACCCATCACCTA
TGGATAAAGGTAAACGACTGAAGATTTTCTATACAACACAAGTGGCTGTAAAACCACCGACGTTTGTTGTATTTGTTAAT
GATCCAGAACTAATGCATTTCTCGTATGAACGTTTCTTAGAAAACCGGATTAGAGAGGCATTTCCGTTTGAGGGTACGCC
AATTCGAGTAATTGCTCGTAAGCGTAAGTAA

Upstream 100 bases:

>100_bases
TTTCTTTTTCAAATCGAACTTTTCATTGCCAGTAAGCTGTTTTACTGATAAACTAAAGAAAGTTTGATAAATAGGATTCG
AGAAAAAAGAAGGTGAAATA

Downstream 100 bases:

>100_bases
AGTTTTCGGGAGGAAAAATATAATGACACAGAAAAAAGTAGCTATTCTTGGCGCTGGAAGTTGGGGAACAGGTCTTGCAC
TTGTCCTAGCTGATAATAAT

Product: GTP-binding protein EngA

Products: NA

Alternate protein names: GTP-binding protein EngA [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MAKPVVAIVGRPNVGKSTIFNRIVGERVSIVEDVPGVTRDRIYNSAEWLGKEFNIIDTGGIDLSDEPFLEQIRAQAEIAI
DEADVIIFITNGREGVTDADEQVAKILYRSNKPIVLAINKVDNPEMRDQIYDFYSLGFGEPYPISGSHGLGLGDMLDAVR
AHFPKEEEEEYPDDTVKFSLIGRPNVGKSSILNALLGEDRVIVSDIAGTTRDAIDTTYTFDGQDYVMIDTAGMRKRGKVY
ESTEKYSVLRAMRAIERSDVVLVVINAEEGIREQDKRIAGYAHDAGRAIIIVVNKWDAINKDEKTINVWTEDIREQFQFL
SYAPIVFVSAKTKQRLNNLFPLINQVSDNHSLRVQSSMLNDVISDAVAMNPSPMDKGKRLKIFYTTQVAVKPPTFVVFVN
DPELMHFSYERFLENRIREAFPFEGTPIRVIARKRK

Sequences:

>Translated_436_residues
MAKPVVAIVGRPNVGKSTIFNRIVGERVSIVEDVPGVTRDRIYNSAEWLGKEFNIIDTGGIDLSDEPFLEQIRAQAEIAI
DEADVIIFITNGREGVTDADEQVAKILYRSNKPIVLAINKVDNPEMRDQIYDFYSLGFGEPYPISGSHGLGLGDMLDAVR
AHFPKEEEEEYPDDTVKFSLIGRPNVGKSSILNALLGEDRVIVSDIAGTTRDAIDTTYTFDGQDYVMIDTAGMRKRGKVY
ESTEKYSVLRAMRAIERSDVVLVVINAEEGIREQDKRIAGYAHDAGRAIIIVVNKWDAINKDEKTINVWTEDIREQFQFL
SYAPIVFVSAKTKQRLNNLFPLINQVSDNHSLRVQSSMLNDVISDAVAMNPSPMDKGKRLKIFYTTQVAVKPPTFVVFVN
DPELMHFSYERFLENRIREAFPFEGTPIRVIARKRK
>Mature_435_residues
AKPVVAIVGRPNVGKSTIFNRIVGERVSIVEDVPGVTRDRIYNSAEWLGKEFNIIDTGGIDLSDEPFLEQIRAQAEIAID
EADVIIFITNGREGVTDADEQVAKILYRSNKPIVLAINKVDNPEMRDQIYDFYSLGFGEPYPISGSHGLGLGDMLDAVRA
HFPKEEEEEYPDDTVKFSLIGRPNVGKSSILNALLGEDRVIVSDIAGTTRDAIDTTYTFDGQDYVMIDTAGMRKRGKVYE
STEKYSVLRAMRAIERSDVVLVVINAEEGIREQDKRIAGYAHDAGRAIIIVVNKWDAINKDEKTINVWTEDIREQFQFLS
YAPIVFVSAKTKQRLNNLFPLINQVSDNHSLRVQSSMLNDVISDAVAMNPSPMDKGKRLKIFYTTQVAVKPPTFVVFVND
PELMHFSYERFLENRIREAFPFEGTPIRVIARKRK

Specific function: GTPase that plays an essential role in the late steps of ribosome biogenesis [H]

COG id: COG1160

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KH-like domain [H]

Homologues:

Organism=Escherichia coli, GI87082120, Length=455, Percent_Identity=41.0989010989011, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1788919, Length=177, Percent_Identity=30.5084745762712, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367268, Length=112, Percent_Identity=41.0714285714286, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=89, Percent_Identity=42.6966292134831, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323665, Length=206, Percent_Identity=33.0097087378641, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI45550813, Length=191, Percent_Identity=34.0314136125654, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016484
- InterPro:   IPR006073
- InterPro:   IPR015946
- InterPro:   IPR002917
- InterPro:   IPR005225 [H]

Pfam domain/function: PF01926 MMR_HSR1 [H]

EC number: NA

Molecular weight: Translated: 49145; Mature: 49013

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPVVAIVGRPNVGKSTIFNRIVGERVSIVEDVPGVTRDRIYNSAEWLGKEFNIIDTGG
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCCCCEEECCC
IDLSDEPFLEQIRAQAEIAIDEADVIIFITNGREGVTDADEQVAKILYRSNKPIVLAINK
CCCCCCHHHHHHHHHHHEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEC
VDNPEMRDQIYDFYSLGFGEPYPISGSHGLGLGDMLDAVRAHFPKEEEEEYPDDTVKFSL
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCEEEEE
IGRPNVGKSSILNALLGEDRVIVSDIAGTTRDAIDTTYTFDGQDYVMIDTAGMRKRGKVY
ECCCCCCHHHHHHHHHCCCCEEEECCCCCCHHCCCCEEEECCCCEEEEECCCCCCCCCCH
ESTEKYSVLRAMRAIERSDVVLVVINAEEGIREQDKRIAGYAHDAGRAIIIVVNKWDAIN
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCEEECCCCEEEEEEECCCCCC
KDEKTINVWTEDIREQFQFLSYAPIVFVSAKTKQRLNNLFPLINQVSDNHSLRVQSSMLN
CCCCEEHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCEEEHHHHHH
DVISDAVAMNPSPMDKGKRLKIFYTTQVAVKPPTFVVFVNDPELMHFSYERFLENRIREA
HHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHH
FPFEGTPIRVIARKRK
CCCCCCCEEEEEECCC
>Mature Secondary Structure 
AKPVVAIVGRPNVGKSTIFNRIVGERVSIVEDVPGVTRDRIYNSAEWLGKEFNIIDTGG
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCCCCEEECCC
IDLSDEPFLEQIRAQAEIAIDEADVIIFITNGREGVTDADEQVAKILYRSNKPIVLAINK
CCCCCCHHHHHHHHHHHEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEC
VDNPEMRDQIYDFYSLGFGEPYPISGSHGLGLGDMLDAVRAHFPKEEEEEYPDDTVKFSL
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCEEEEE
IGRPNVGKSSILNALLGEDRVIVSDIAGTTRDAIDTTYTFDGQDYVMIDTAGMRKRGKVY
ECCCCCCHHHHHHHHHCCCCEEEECCCCCCHHCCCCEEEECCCCEEEEECCCCCCCCCCH
ESTEKYSVLRAMRAIERSDVVLVVINAEEGIREQDKRIAGYAHDAGRAIIIVVNKWDAIN
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCEEECCCCEEEEEEECCCCCC
KDEKTINVWTEDIREQFQFLSYAPIVFVSAKTKQRLNNLFPLINQVSDNHSLRVQSSMLN
CCCCEEHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCEEEHHHHHH
DVISDAVAMNPSPMDKGKRLKIFYTTQVAVKPPTFVVFVNDPELMHFSYERFLENRIREA
HHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHH
FPFEGTPIRVIARKRK
CCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]