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The map label for this gene is aroF

Identifier: 226224532

GI number: 226224532

Start: 1991010

End: 1992176

Strand: Reverse

Name: aroF

Synonym: Lm4b_01945

Alternate gene names: 226224532

Gene position: 1992176-1991010 (Counterclockwise)

Preceding gene: 226224533

Following gene: 226224531

Centisome position: 68.4

GC content: 43.7

Gene sequence:

>1167_bases
ATGAGATACTTAACGGCAGGAGAATCACACGGACCGGGGCTTACAACAATTATTGAAGGGTTGCCAGCAGGAATGCCTTT
ACTAGCAGAAGATGTAAATAAAGAACTAAAAAGAAGACAAGGTGGGCATGGTCGTGGGGCACGGATGCGTATCGAAAAAG
ACCAAGTACAAATCACAGCAGGGATTCGTCATGGTAAAACATTAGGCGCACCTGTAGCAATGTTTGTTGAAAATAAAGAC
TGGAAGCATTGGGAAACGGTTATGAGCATTGAACCAGTTCCAGAAAAAAATGAAAAATCCCGTCGCGTATCCCGTCCACG
TCCTGGACATGCGGACTTAGTTGGCGGTATGAAATATGGTCATAACGATATGCGTAACGTACTAGAACGTTCTAGCGCGC
GCGAAACGACTGTCCGTGTGGCAGCTGGAGCAGTAGCGAAAAAATTATTGCATGAACTTGGCATTGAAGTAGCTGGTCAT
GTACTTGAAATTGGTGGCACTCGTGCGAACTTAACACGTGATTACGCGGTTAGAGAAATCCAAGAAACATCCGAAGCTTC
TCCAGTGCGTTGTTTAGACGGCGTAGCGGCAGAAGAAATGATGCAAAAAATTGATGATGCGAAGAAAAATGGCGACACCA
TTGGCGGTATCGTAGAAGTTGTAGTTGGTGGTGTCCCAGCTGGACTTGGTAGCTACGTACAGTGGGACAAAAAACTAGAT
GCAAAAATTGCGCGTGCGATTGTAAGTATTAATGCATTTAAAGGCGCTGAATTCGGTGTCGGTTTTGAAGCTGCTAGAAA
ACCTGGTAGTGAAGTGATGGATGAAATTTTATGGAGTAAAGAAGATGGCTACACGAGACGTACAAATAACCTTGGTGGAT
TCGAAGGCGGAATGACCAATGGTATGCCAATTGTTGTTCGTGGCGTAATGAAGCCAATTCCAACATTATACAAACCACTT
CAAAGTGTGGATATTGATTCAAAAGAAACGTTTAATGCAAGTGTAGAACGTTCGGATAGTTGTGCAGTACCTGCTGCAAG
TGTGGTAGCTGAAGCTGTTGTCGCTTGGGAAGTTGCAGTTGCTGTGTTAGAAAAATTTGATGGTGATCGTTTTGACACAC
TTAAAAAACATGTGGAGGAACACCGAAACTTAACGAAGGAGTTTTAA

Upstream 100 bases:

>100_bases
TCTCGCGTGGCATATGTTAAGATATTACACATATACTTTAAAGCGCTAAAATATAAACGCTAGAAAATCTAGCTGAAATA
GAAAAAGGAGAGATGAGCAG

Downstream 100 bases:

>100_bases
ACATGCCAGAAATTACAGTCCGTGCTAAAAGTAAAACATACCCAGTATATATTAATGAATTCGCATTAGAAGATGTCCGG
GAAAAGTGGACGGAGAGCCT

Product: chorismate synthase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate phospholyase [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MRYLTAGESHGPGLTTIIEGLPAGMPLLAEDVNKELKRRQGGHGRGARMRIEKDQVQITAGIRHGKTLGAPVAMFVENKD
WKHWETVMSIEPVPEKNEKSRRVSRPRPGHADLVGGMKYGHNDMRNVLERSSARETTVRVAAGAVAKKLLHELGIEVAGH
VLEIGGTRANLTRDYAVREIQETSEASPVRCLDGVAAEEMMQKIDDAKKNGDTIGGIVEVVVGGVPAGLGSYVQWDKKLD
AKIARAIVSINAFKGAEFGVGFEAARKPGSEVMDEILWSKEDGYTRRTNNLGGFEGGMTNGMPIVVRGVMKPIPTLYKPL
QSVDIDSKETFNASVERSDSCAVPAASVVAEAVVAWEVAVAVLEKFDGDRFDTLKKHVEEHRNLTKEF

Sequences:

>Translated_388_residues
MRYLTAGESHGPGLTTIIEGLPAGMPLLAEDVNKELKRRQGGHGRGARMRIEKDQVQITAGIRHGKTLGAPVAMFVENKD
WKHWETVMSIEPVPEKNEKSRRVSRPRPGHADLVGGMKYGHNDMRNVLERSSARETTVRVAAGAVAKKLLHELGIEVAGH
VLEIGGTRANLTRDYAVREIQETSEASPVRCLDGVAAEEMMQKIDDAKKNGDTIGGIVEVVVGGVPAGLGSYVQWDKKLD
AKIARAIVSINAFKGAEFGVGFEAARKPGSEVMDEILWSKEDGYTRRTNNLGGFEGGMTNGMPIVVRGVMKPIPTLYKPL
QSVDIDSKETFNASVERSDSCAVPAASVVAEAVVAWEVAVAVLEKFDGDRFDTLKKHVEEHRNLTKEF
>Mature_388_residues
MRYLTAGESHGPGLTTIIEGLPAGMPLLAEDVNKELKRRQGGHGRGARMRIEKDQVQITAGIRHGKTLGAPVAMFVENKD
WKHWETVMSIEPVPEKNEKSRRVSRPRPGHADLVGGMKYGHNDMRNVLERSSARETTVRVAAGAVAKKLLHELGIEVAGH
VLEIGGTRANLTRDYAVREIQETSEASPVRCLDGVAAEEMMQKIDDAKKNGDTIGGIVEVVVGGVPAGLGSYVQWDKKLD
AKIARAIVSINAFKGAEFGVGFEAARKPGSEVMDEILWSKEDGYTRRTNNLGGFEGGMTNGMPIVVRGVMKPIPTLYKPL
QSVDIDSKETFNASVERSDSCAVPAASVVAEAVVAWEVAVAVLEKFDGDRFDTLKKHVEEHRNLTKEF

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; seventh step. [C]

COG id: COG0082

COG function: function code E; Chorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788669, Length=356, Percent_Identity=37.6404494382022, Blast_Score=175, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6321290, Length=374, Percent_Identity=35.5614973262032, Blast_Score=196, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000453
- InterPro:   IPR020541 [H]

Pfam domain/function: PF01264 Chorismate_synt [H]

EC number: =4.2.3.5 [H]

Molecular weight: Translated: 42258; Mature: 42258

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00787 CHORISMATE_SYNTHASE_1 ; PS00788 CHORISMATE_SYNTHASE_2 ; PS00789 CHORISMATE_SYNTHASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLTAGESHGPGLTTIIEGLPAGMPLLAEDVNKELKRRQGGHGRGARMRIEKDQVQITA
CCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHEEEEE
GIRHGKTLGAPVAMFVENKDWKHWETVMSIEPVPEKNEKSRRVSRPRPGHADLVGGMKYG
CCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCCC
HNDMRNVLERSSARETTVRVAAGAVAKKLLHELGIEVAGHVLEIGGTRANLTRDYAVREI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEECCCHHHHHHHHHHHHH
QETSEASPVRCLDGVAAEEMMQKIDDAKKNGDTIGGIVEVVVGGVPAGLGSYVQWDKKLD
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AKIARAIVSINAFKGAEFGVGFEAARKPGSEVMDEILWSKEDGYTRRTNNLGGFEGGMTN
HHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GMPIVVRGVMKPIPTLYKPLQSVDIDSKETFNASVERSDSCAVPAASVVAEAVVAWEVAV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AVLEKFDGDRFDTLKKHVEEHRNLTKEF
HHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRYLTAGESHGPGLTTIIEGLPAGMPLLAEDVNKELKRRQGGHGRGARMRIEKDQVQITA
CCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHEEEEE
GIRHGKTLGAPVAMFVENKDWKHWETVMSIEPVPEKNEKSRRVSRPRPGHADLVGGMKYG
CCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCCC
HNDMRNVLERSSARETTVRVAAGAVAKKLLHELGIEVAGHVLEIGGTRANLTRDYAVREI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEECCCHHHHHHHHHHHHH
QETSEASPVRCLDGVAAEEMMQKIDDAKKNGDTIGGIVEVVVGGVPAGLGSYVQWDKKLD
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AKIARAIVSINAFKGAEFGVGFEAARKPGSEVMDEILWSKEDGYTRRTNNLGGFEGGMTN
HHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GMPIVVRGVMKPIPTLYKPLQSVDIDSKETFNASVERSDSCAVPAASVVAEAVVAWEVAV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AVLEKFDGDRFDTLKKHVEEHRNLTKEF
HHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]