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The map label for this gene is thiN [H]

Identifier: 226224420

GI number: 226224420

Start: 1877492

End: 1878136

Strand: Reverse

Name: thiN [H]

Synonym: Lm4b_01833

Alternate gene names: 226224420

Gene position: 1878136-1877492 (Counterclockwise)

Preceding gene: 226224421

Following gene: 226224418

Centisome position: 64.48

GC content: 40.16

Gene sequence:

>645_bases
ATGAAAATAATTAATATCATGGTTGGTGGACCTGCGTCCGAAATTCCTGATTTAGCACAATATACGAGCGCAGAGATTAG
TTGGATTGGCGTGGACCGCGGAGCAAAACGTTTGTTAGACTATGGAATTGTACCGACCGTTGCGATGGGAGATTTTGATT
CACTCTCTAAAGAAGAACTGGCTCATTTGAAAACCAAAGTAGCAGATGTACACGAATTTCCTGCAGAAAAAGATGAAACA
GATACGGAAATTGGATTAAGTTGGGCGATGGAGCAAAATCCAGATAAGATACGCATTTTTGGTGCAACAGGAGGCCGCTT
AGACCATCTACTTGCGAATCTAATGATGTTGACCAAGCCGAAATTTTTAAGCGCTGTGCCAGTTGTAGAAATGATTGACC
GTCATAACTACATCAAAATGTATATGCCAGGTAGCTACACTATTGAAAAACTACCTGATAAGAAATATGTCGCATTTACA
ACGATGAAAGACGTGACAGGACTGACACTAAAAGGATTCGCCTACCCGCTCGAAAATGCCACTTACCCAGTAGGTTCAGC
TCTATCAAGTAATGAGTTTGTGGGACAAATGGGGGAATTTTCCTTTAAAAGTGGAATGATTTTATTAACACAAAGTAATG
ATTAA

Upstream 100 bases:

>100_bases
ACCAATTGATAAATTACGTGCCATTGTAGGCGAGTAAACTAATAGTAGGGCTGGGACCTCTGTTCCAGTCTTATTTGTTA
GGGAAGAAGGGGACTCAAGT

Downstream 100 bases:

>100_bases
CTAGAAATACAGGTGCACTCGGCCTGTATTTTTTTATTTTAAGGCAAAGAAAAAATGCACCGACATAATTGTCGCTGCAC
TTGATTTCTCCTTCAATTAA

Product: thiamin pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKIINIMVGGPASEIPDLAQYTSAEISWIGVDRGAKRLLDYGIVPTVAMGDFDSLSKEELAHLKTKVADVHEFPAEKDET
DTEIGLSWAMEQNPDKIRIFGATGGRLDHLLANLMMLTKPKFLSAVPVVEMIDRHNYIKMYMPGSYTIEKLPDKKYVAFT
TMKDVTGLTLKGFAYPLENATYPVGSALSSNEFVGQMGEFSFKSGMILLTQSND

Sequences:

>Translated_214_residues
MKIINIMVGGPASEIPDLAQYTSAEISWIGVDRGAKRLLDYGIVPTVAMGDFDSLSKEELAHLKTKVADVHEFPAEKDET
DTEIGLSWAMEQNPDKIRIFGATGGRLDHLLANLMMLTKPKFLSAVPVVEMIDRHNYIKMYMPGSYTIEKLPDKKYVAFT
TMKDVTGLTLKGFAYPLENATYPVGSALSSNEFVGQMGEFSFKSGMILLTQSND
>Mature_214_residues
MKIINIMVGGPASEIPDLAQYTSAEISWIGVDRGAKRLLDYGIVPTVAMGDFDSLSKEELAHLKTKVADVHEFPAEKDET
DTEIGLSWAMEQNPDKIRIFGATGGRLDHLLANLMMLTKPKFLSAVPVVEMIDRHNYIKMYMPGSYTIEKLPDKKYVAFT
TMKDVTGLTLKGFAYPLENATYPVGSALSSNEFVGQMGEFSFKSGMILLTQSND

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 23652; Mature: 23652

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIINIMVGGPASEIPDLAQYTSAEISWIGVDRGAKRLLDYGIVPTVAMGDFDSLSKEEL
CEEEEEEECCCHHHCCHHHHHCCCCEEEEECCHHHHHHHHCCCCCEEECCCCCCCCHHHH
AHLKTKVADVHEFPAEKDETDTEIGLSWAMEQNPDKIRIFGATGGRLDHLLANLMMLTKP
HHHHHHHHHHHHCCCCCCCCCCHHCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCC
KFLSAVPVVEMIDRHNYIKMYMPGSYTIEKLPDKKYVAFTTMKDVTGLTLKGFAYPLENA
CHHHHHHHHHHHCCCCEEEEEECCCEEHCCCCCCCEEEEEECCCCCCCEEECEEEECCCC
TYPVGSALSSNEFVGQMGEFSFKSGMILLTQSND
CCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MKIINIMVGGPASEIPDLAQYTSAEISWIGVDRGAKRLLDYGIVPTVAMGDFDSLSKEEL
CEEEEEEECCCHHHCCHHHHHCCCCEEEEECCHHHHHHHHCCCCCEEECCCCCCCCHHHH
AHLKTKVADVHEFPAEKDETDTEIGLSWAMEQNPDKIRIFGATGGRLDHLLANLMMLTKP
HHHHHHHHHHHHCCCCCCCCCCHHCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCC
KFLSAVPVVEMIDRHNYIKMYMPGSYTIEKLPDKKYVAFTTMKDVTGLTLKGFAYPLENA
CHHHHHHHHHHHCCCCEEEEEECCCEEHCCCCCCCEEEEEECCCCCCCEEECEEEECCCC
TYPVGSALSSNEFVGQMGEFSFKSGMILLTQSND
CCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]