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The map label for this gene is recG [H]

Identifier: 226224414

GI number: 226224414

Start: 1871101

End: 1873149

Strand: Reverse

Name: recG [H]

Synonym: Lm4b_01827

Alternate gene names: 226224414

Gene position: 1873149-1871101 (Counterclockwise)

Preceding gene: 226224415

Following gene: 226224413

Centisome position: 64.31

GC content: 41.0

Gene sequence:

>2049_bases
GTGAGTGAACTTAAACGAATCCCGACAACTGAAATCAAAGGAATTGGCGAAGAAACAGCGAAAACGTTAAAAGAGTTAGG
TTTATCCACCGTGCATGATTTGCTTTGGAACTTCCCGTATCGCTATGAGGATTATCGGTTGCGGGATTTATCAGAAGTGG
CGCATGAAGAACGGATTACCATTCAAGGCGAAGTCCTAACAGAGGCAACCGTTGCTTTCTACGGTCGCAAAAAATCCAAG
CTCTCTTTCCGCGTCTCAACTGAAGGGCAAGTCATCAAAATCGATTTTTTCAACCAACCCTATTTAAAAAGTAAAATAAG
TGTTGGCGAAACAGTGACAATCTCTGGCAAATGGGATAAGAGCCGCGCCCAAGTTACAGCCAGCAAAATAAAAATAGGCG
CTGTCGAAAGTGAAGAAGAACTAGAAGGCGTTTACCGTCTAAAAGGAACGCTACGCAATAAAACAATGCAAAAATATACG
AGACTTGCTTTTAATAGTTATAGTCAAGATATAGAAGAAGTTATTCCAAGCAATTTATTAGAAAAGTATCAATTAATGGA
TCGCCTAGAAGCAGTGCGCATTCTGCATTTTCCTAAAAATAATGAGGAATTGAAACAAGCTAGGCGCCGAATGGTCTACG
AAGAATTCTTGTTATTCCAATTGAAAATGCAGTTTTTCCGTAAAATTGAACGCGAGAAATCAGGTGGAATTTCGATTAAT
TACGATGTAGAAGATTTGCGCCATTACATTGATTCCTTGCCATTTCCACTAACAAAAGCACAAAAACGAGTAGTCAATGA
AATTTGCGGTGATATGTTATCACACTTTCATATGAATCGTTTGCTACAAGGAGACGTGGGATCGGGTAAAACAGTTGTAG
CATCTATTGCCATGTATGCAGCTGCCAAAAGTGGCTTCCAAAGCGCACTCATGGTGCCAACCGAAATTTTAGCCGAGCAA
CATGCGAATTCCTTAGTAGAGCTGTTGCAACCTTTTGATATTACAGTTGGCTTACTAACGAGTAGTGTGAAAGGCAAACA
GCGCAGAGAATTACTAGCAATGCTTGAAAATGGCTCCATTGATGTTTTAATCGGAACGCATGCATTAATTCAAGACGAAG
TAATTTATCATCGCCTAGGTTTAGTTATTACTGACGAGCAACATCGATTTGGCGTAGCGCAGCGTCGTGTTCTTCGTGAA
AAAGGGGAATATCCGGATGTTTTATTTATGACCGCAACCCCAATTCCTAGAACGCTTGCCATTACAGCATTTGGCGAAAT
GGATGTATCGATTATCGATGAACTCCCAGCTGGTCGAAAAGAAATTGAAACTTACTGGGTAAAACATCAAATGCTTGATC
GCGTTATCGGTTTTATGGAAAAAGAAATCGACAAAGGCCACCAAGTCTACATCATTTGTCCGCTAATTGAAGAATCTGAA
AAACTAGATGTGCAAAATGCGATAGACGTTTTCAACATTCTACAAAATAAATGGGGCACTAAATATATTCCTGGTCTAAT
GCACGGTAAACTATTACCAGCAGATAAAGAGCAAATTATGCGCGACTTTAATAATAAAAAAATTGATTGCCTTGTTTCGA
CCACAGTAGTGGAAGTTGGTGTAAACGTGCCAAACGCTACCATGATGGTCATTTACGATGCCGACCGTTTTGGTTTAGCG
CAGTTGCATCAACTTCGGGGCCGAGTTGGGCGCGGAGCAGATCAGTCTTACTGTATTTTAATTGCTGACCCAAAAACCGA
AGTCGGCAAAGAACGTATGATGATTATGTCAGAAACAAACGATGGCTTCGTTGTCAGCGAACGCGACCTAGAACTTCGTG
GCCCAGGAGATTTCTTCGGCAGAAAACAAAGTGGTGTTCCAGAATTCAAAGTAGCAGATATGGTTCACGATTACCGGGTA
CTGGAGATCGCCCGCCAAGATGCCGTTCACATGATCTTTGAAGAAGATATGTTAGAGAACAAAAACTACGAAAAATTAGT
AGCACTTCTGGAGGAAGAAGGCGTCTTCACGGAGCAAAAACTAGATTAA

Upstream 100 bases:

>100_bases
CCAAGCTCTTTAAGAGAAACGGCAGAAGGAGGATTGGCGGCTACACCGACTGGACGTGCAATTCAGCGGAAGATTTTTGG
ACTGTCGCCAGAAGATAAGC

Downstream 100 bases:

>100_bases
ACCTATGTAACAATCTTTAAAAAAACTTGCAACTTGGCTTGATTTTTAATAACATATTAAAGTACCAATAAATACAAAGG
AAGTTCTGAATGTTATCCTA

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 682; Mature: 681

Protein sequence:

>682_residues
MSELKRIPTTEIKGIGEETAKTLKELGLSTVHDLLWNFPYRYEDYRLRDLSEVAHEERITIQGEVLTEATVAFYGRKKSK
LSFRVSTEGQVIKIDFFNQPYLKSKISVGETVTISGKWDKSRAQVTASKIKIGAVESEEELEGVYRLKGTLRNKTMQKYT
RLAFNSYSQDIEEVIPSNLLEKYQLMDRLEAVRILHFPKNNEELKQARRRMVYEEFLLFQLKMQFFRKIEREKSGGISIN
YDVEDLRHYIDSLPFPLTKAQKRVVNEICGDMLSHFHMNRLLQGDVGSGKTVVASIAMYAAAKSGFQSALMVPTEILAEQ
HANSLVELLQPFDITVGLLTSSVKGKQRRELLAMLENGSIDVLIGTHALIQDEVIYHRLGLVITDEQHRFGVAQRRVLRE
KGEYPDVLFMTATPIPRTLAITAFGEMDVSIIDELPAGRKEIETYWVKHQMLDRVIGFMEKEIDKGHQVYIICPLIEESE
KLDVQNAIDVFNILQNKWGTKYIPGLMHGKLLPADKEQIMRDFNNKKIDCLVSTTVVEVGVNVPNATMMVIYDADRFGLA
QLHQLRGRVGRGADQSYCILIADPKTEVGKERMMIMSETNDGFVVSERDLELRGPGDFFGRKQSGVPEFKVADMVHDYRV
LEIARQDAVHMIFEEDMLENKNYEKLVALLEEEGVFTEQKLD

Sequences:

>Translated_682_residues
MSELKRIPTTEIKGIGEETAKTLKELGLSTVHDLLWNFPYRYEDYRLRDLSEVAHEERITIQGEVLTEATVAFYGRKKSK
LSFRVSTEGQVIKIDFFNQPYLKSKISVGETVTISGKWDKSRAQVTASKIKIGAVESEEELEGVYRLKGTLRNKTMQKYT
RLAFNSYSQDIEEVIPSNLLEKYQLMDRLEAVRILHFPKNNEELKQARRRMVYEEFLLFQLKMQFFRKIEREKSGGISIN
YDVEDLRHYIDSLPFPLTKAQKRVVNEICGDMLSHFHMNRLLQGDVGSGKTVVASIAMYAAAKSGFQSALMVPTEILAEQ
HANSLVELLQPFDITVGLLTSSVKGKQRRELLAMLENGSIDVLIGTHALIQDEVIYHRLGLVITDEQHRFGVAQRRVLRE
KGEYPDVLFMTATPIPRTLAITAFGEMDVSIIDELPAGRKEIETYWVKHQMLDRVIGFMEKEIDKGHQVYIICPLIEESE
KLDVQNAIDVFNILQNKWGTKYIPGLMHGKLLPADKEQIMRDFNNKKIDCLVSTTVVEVGVNVPNATMMVIYDADRFGLA
QLHQLRGRVGRGADQSYCILIADPKTEVGKERMMIMSETNDGFVVSERDLELRGPGDFFGRKQSGVPEFKVADMVHDYRV
LEIARQDAVHMIFEEDMLENKNYEKLVALLEEEGVFTEQKLD
>Mature_681_residues
SELKRIPTTEIKGIGEETAKTLKELGLSTVHDLLWNFPYRYEDYRLRDLSEVAHEERITIQGEVLTEATVAFYGRKKSKL
SFRVSTEGQVIKIDFFNQPYLKSKISVGETVTISGKWDKSRAQVTASKIKIGAVESEEELEGVYRLKGTLRNKTMQKYTR
LAFNSYSQDIEEVIPSNLLEKYQLMDRLEAVRILHFPKNNEELKQARRRMVYEEFLLFQLKMQFFRKIEREKSGGISINY
DVEDLRHYIDSLPFPLTKAQKRVVNEICGDMLSHFHMNRLLQGDVGSGKTVVASIAMYAAAKSGFQSALMVPTEILAEQH
ANSLVELLQPFDITVGLLTSSVKGKQRRELLAMLENGSIDVLIGTHALIQDEVIYHRLGLVITDEQHRFGVAQRRVLREK
GEYPDVLFMTATPIPRTLAITAFGEMDVSIIDELPAGRKEIETYWVKHQMLDRVIGFMEKEIDKGHQVYIICPLIEESEK
LDVQNAIDVFNILQNKWGTKYIPGLMHGKLLPADKEQIMRDFNNKKIDCLVSTTVVEVGVNVPNATMMVIYDADRFGLAQ
LHQLRGRVGRGADQSYCILIADPKTEVGKERMMIMSETNDGFVVSERDLELRGPGDFFGRKQSGVPEFKVADMVHDYRVL
EIARQDAVHMIFEEDMLENKNYEKLVALLEEEGVFTEQKLD

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=674, Percent_Identity=38.5756676557863, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1787357, Length=406, Percent_Identity=35.4679802955665, Blast_Score=250, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78023; Mature: 77892

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELKRIPTTEIKGIGEETAKTLKELGLSTVHDLLWNFPYRYEDYRLRDLSEVAHEERIT
CCCCCCCCCHHHCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEE
IQGEVLTEATVAFYGRKKSKLSFRVSTEGQVIKIDFFNQPYLKSKISVGETVTISGKWDK
EEEHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCHHHHHCCCCCEEEEECCCCC
SRAQVTASKIKIGAVESEEELEGVYRLKGTLRNKTMQKYTRLAFNSYSQDIEEVIPSNLL
HHHHEEHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKYQLMDRLEAVRILHFPKNNEELKQARRRMVYEEFLLFQLKMQFFRKIEREKSGGISIN
HHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
YDVEDLRHYIDSLPFPLTKAQKRVVNEICGDMLSHFHMNRLLQGDVGSGKTVVASIAMYA
ECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AAKSGFQSALMVPTEILAEQHANSLVELLQPFDITVGLLTSSVKGKQRRELLAMLENGSI
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCE
DVLIGTHALIQDEVIYHRLGLVITDEQHRFGVAQRRVLREKGEYPDVLFMTATPIPRTLA
EEEECCHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEE
ITAFGEMDVSIIDELPAGRKEIETYWVKHQMLDRVIGFMEKEIDKGHQVYIICPLIEESE
EEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
KLDVQNAIDVFNILQNKWGTKYIPGLMHGKLLPADKEQIMRDFNNKKIDCLVSTTVVEVG
CCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHCCCCEEEEEEEEEEEEEC
VNVPNATMMVIYDADRFGLAQLHQLRGRVGRGADQSYCILIADPKTEVGKERMMIMSETN
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHCCCCCEEEEEECC
DGFVVSERDLELRGPGDFFGRKQSGVPEFKVADMVHDYRVLEIARQDAVHMIFEEDMLEN
CCEEEECCCEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KNYEKLVALLEEEGVFTEQKLD
CCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SELKRIPTTEIKGIGEETAKTLKELGLSTVHDLLWNFPYRYEDYRLRDLSEVAHEERIT
CCCCCCCCHHHCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEE
IQGEVLTEATVAFYGRKKSKLSFRVSTEGQVIKIDFFNQPYLKSKISVGETVTISGKWDK
EEEHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCHHHHHCCCCCEEEEECCCCC
SRAQVTASKIKIGAVESEEELEGVYRLKGTLRNKTMQKYTRLAFNSYSQDIEEVIPSNLL
HHHHEEHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKYQLMDRLEAVRILHFPKNNEELKQARRRMVYEEFLLFQLKMQFFRKIEREKSGGISIN
HHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
YDVEDLRHYIDSLPFPLTKAQKRVVNEICGDMLSHFHMNRLLQGDVGSGKTVVASIAMYA
ECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AAKSGFQSALMVPTEILAEQHANSLVELLQPFDITVGLLTSSVKGKQRRELLAMLENGSI
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCE
DVLIGTHALIQDEVIYHRLGLVITDEQHRFGVAQRRVLREKGEYPDVLFMTATPIPRTLA
EEEECCHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEE
ITAFGEMDVSIIDELPAGRKEIETYWVKHQMLDRVIGFMEKEIDKGHQVYIICPLIEESE
EEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
KLDVQNAIDVFNILQNKWGTKYIPGLMHGKLLPADKEQIMRDFNNKKIDCLVSTTVVEVG
CCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHCCCCEEEEEEEEEEEEEC
VNVPNATMMVIYDADRFGLAQLHQLRGRVGRGADQSYCILIADPKTEVGKERMMIMSETN
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHCCCCCEEEEEECC
DGFVVSERDLELRGPGDFFGRKQSGVPEFKVADMVHDYRVLEIARQDAVHMIFEEDMLEN
CCEEEECCCEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KNYEKLVALLEEEGVFTEQKLD
CCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]