Click here to switch to the map view.

The map label for this gene is pcrA

Identifier: 226224362

GI number: 226224362

Start: 1816417

End: 1818612

Strand: Reverse

Name: pcrA

Synonym: Lm4b_01773

Alternate gene names: 226224362

Gene position: 1818612-1816417 (Counterclockwise)

Preceding gene: 226224363

Following gene: 226224361

Centisome position: 62.44

GC content: 39.85

Gene sequence:

>2196_bases
TTGAATGCAAAAGAATTAGTGGACGGATTGAATCCGGAACAACGAAGAGCAGTAGAAAGTACAGAAGGACCTTTATTAAT
TATGGCTGGTGCCGGAAGTGGGAAAACACGAGTTTTGACCCATCGGATAGCTTATTTAGTGAGAGAACGTGGTGTAAATC
CTTATAATATTTTAGCGATTACGTTTACGAACAAAGCGGCTCGCGAGATGAAGTCACGGATTGGCAATTTAATGGGCGGA
GAAGCAGAATCTATTTGGATATCTACTTTTCACTCGATGTGCGTACGAATTTTACGTCGCGATATTGACCGGATTGGCTA
TGAACGTAATTTTACTATTTTAGATGGCAGTGACCAATTATCGGTTATTAAAGGCATTTTGAAAGAAAAGAATGTCGACC
CTAAGAAGTTTGAGCCACGGGGGATTTTGGCTTCTATTAGCAATGCCAAAAATGAACTGATAACTGCAAGCGAATATATT
AAAGAAGCAAGTGGTTTCTATGACAAAATGGTCGGCGAAGTCTACGAAAAATACGAGAAGAAACTTAAGAAAAATCAAGC
ACTCGATTTTGATGATTTAATTATGGTCACCATTCAATTATTTGAACGTGTGCCCGATGTTTTAGAATATTATCAACGTA
AATTTCAGTATATTCACGTGGATGAGTACCAAGATACCAACCACGCGCAGTATTTACTCGTGAAACAGCTTGCTTCTCGT
TTCAAAAACTTATGCGTCGTAGGTGACTCGGATCAATCTATTTATGGTTGGCGCGGGGCGGACATTAGTAACATCATGTC
CTTCGAAAAAGATTACCCCAATGCGAAAACCATTTTGCTAGAAGAAAATTACCGTTCGACAAAACGTATTTTAGAAGCGG
CAAACCGTGTTATCGAAAATAATGGTAATCGTAAACCGAAAAATCTTTGGACAAGTAACGCAGAAGGGAAAAAGATTTTT
TATCATAAAGCATTAACGGAAAAAGAAGAAGCAGCCTATGTAGTTATGAAAATTCAAGAAGAAGTAAATAACTCGAATCG
ACCACTTTCTGACTTTGCGATTCTTTATAGAACTAACGCCCAGTCCCGTGTCATGGAAGAATATTTCATGAAGTCGAATA
TGGCGTACACGATGGTCGGCGGCACGAAGTTCTATGACAGAAAAGAAATTAAAGACATTTTGGCGTATTTACGTCTTATT
AGTAATAATGAAGATGATATTAGTTTGACACGTATCGTTAACGTTCCAAAACGCGGCGTTGGACCAGGTACTTTAGATAA
ACTAAATAATGTGGCGACCACGTATGATTTGAGCTTGTTTGAAGTCCTTAACCGCATCGAACTAGCGGGAATTTCGCCTA
AAATAAGCAAAGACTTAGTTGCGTTTCATGATTTGATTCGCGGCTTCACGCAAATGCAAGATTTCTTATCCGTAACCGAA
CTCGTAGAAGAAATCCTTGAAAAAACAGGCTACCGCGCAATGCTAAAAAATGAACGAACCATTGAAGCGCAAACACGTTT
AGAAAATATCGACGAGTTTTTATCTGTAACACAAAACTTTGAAAAGGAAAATGACGATAAAACGTTAATTGCCTTTTTAA
CTGATTTAGCACTCGTTGCTGACGTCGATAAGTTAGAAGAAGACAATGAAGAACAAAACGGGGCTGTCACACTGATGACA
CTTCACTCTGCCAAAGGGCTAGAATTCCCAGTTGTCTTTTTGGTTGGGATGGAAGAAGGGATTTTCCCACATTCTAGAGC
CATTTATGAGGAAGATGAAATGGAAGAAGAGCGTCGACTTGCTTACGTTGGTATTACTCGAGCGGAAGAAGAACTTTTCC
TAACGAGCGCTTACTCACGGATGCTTTATGGTCGTCCGTCCTCTAATCAGGAATCTCGTTTTATTGGAGAAATACCTCGA
GACTTACTTGAGTTAGGTAATGAAAACAAATTAAAAGCAGACAAACCATATGCCAAACCGCGTATGCCACAAAAAACAAC
TACTGCGTATAAATCCTCTGGAGCAGAAACGCTTGGTTGGACAGTCGGCGATAAAGCCAGTCATAAAAAATGGGGCGTAG
GAACAGTCGTCAGTGTCAAAGGGGAAGGTAGCGGAATGGAACTAGATATTGCATTCCCAAGCCCAACTGGCGTCAAACGA
TTGCTCGCAGAATTTGCGCCAATTGAAAAAGTATAA

Upstream 100 bases:

>100_bases
GAAACCGCAACTATTTTTAGGAAGAAAACGGTTTGATTATAAAGTGTAGTATGATAAAATAAGAACAAATGTTTGTATGG
TGGTGTATAGGAGAATGCAA

Downstream 100 bases:

>100_bases
ATTTGGAAAAAGCAGGTGAAGAATGATGGCTGATAAAAAAAGGTATGAGGAACTAATCAACATACTTGATCAGTACAGCT
ATGATTATTATGTAATTGAT

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MNAKELVDGLNPEQRRAVESTEGPLLIMAGAGSGKTRVLTHRIAYLVRERGVNPYNILAITFTNKAAREMKSRIGNLMGG
EAESIWISTFHSMCVRILRRDIDRIGYERNFTILDGSDQLSVIKGILKEKNVDPKKFEPRGILASISNAKNELITASEYI
KEASGFYDKMVGEVYEKYEKKLKKNQALDFDDLIMVTIQLFERVPDVLEYYQRKFQYIHVDEYQDTNHAQYLLVKQLASR
FKNLCVVGDSDQSIYGWRGADISNIMSFEKDYPNAKTILLEENYRSTKRILEAANRVIENNGNRKPKNLWTSNAEGKKIF
YHKALTEKEEAAYVVMKIQEEVNNSNRPLSDFAILYRTNAQSRVMEEYFMKSNMAYTMVGGTKFYDRKEIKDILAYLRLI
SNNEDDISLTRIVNVPKRGVGPGTLDKLNNVATTYDLSLFEVLNRIELAGISPKISKDLVAFHDLIRGFTQMQDFLSVTE
LVEEILEKTGYRAMLKNERTIEAQTRLENIDEFLSVTQNFEKENDDKTLIAFLTDLALVADVDKLEEDNEEQNGAVTLMT
LHSAKGLEFPVVFLVGMEEGIFPHSRAIYEEDEMEEERRLAYVGITRAEEELFLTSAYSRMLYGRPSSNQESRFIGEIPR
DLLELGNENKLKADKPYAKPRMPQKTTTAYKSSGAETLGWTVGDKASHKKWGVGTVVSVKGEGSGMELDIAFPSPTGVKR
LLAEFAPIEKV

Sequences:

>Translated_731_residues
MNAKELVDGLNPEQRRAVESTEGPLLIMAGAGSGKTRVLTHRIAYLVRERGVNPYNILAITFTNKAAREMKSRIGNLMGG
EAESIWISTFHSMCVRILRRDIDRIGYERNFTILDGSDQLSVIKGILKEKNVDPKKFEPRGILASISNAKNELITASEYI
KEASGFYDKMVGEVYEKYEKKLKKNQALDFDDLIMVTIQLFERVPDVLEYYQRKFQYIHVDEYQDTNHAQYLLVKQLASR
FKNLCVVGDSDQSIYGWRGADISNIMSFEKDYPNAKTILLEENYRSTKRILEAANRVIENNGNRKPKNLWTSNAEGKKIF
YHKALTEKEEAAYVVMKIQEEVNNSNRPLSDFAILYRTNAQSRVMEEYFMKSNMAYTMVGGTKFYDRKEIKDILAYLRLI
SNNEDDISLTRIVNVPKRGVGPGTLDKLNNVATTYDLSLFEVLNRIELAGISPKISKDLVAFHDLIRGFTQMQDFLSVTE
LVEEILEKTGYRAMLKNERTIEAQTRLENIDEFLSVTQNFEKENDDKTLIAFLTDLALVADVDKLEEDNEEQNGAVTLMT
LHSAKGLEFPVVFLVGMEEGIFPHSRAIYEEDEMEEERRLAYVGITRAEEELFLTSAYSRMLYGRPSSNQESRFIGEIPR
DLLELGNENKLKADKPYAKPRMPQKTTTAYKSSGAETLGWTVGDKASHKKWGVGTVVSVKGEGSGMELDIAFPSPTGVKR
LLAEFAPIEKV
>Mature_731_residues
MNAKELVDGLNPEQRRAVESTEGPLLIMAGAGSGKTRVLTHRIAYLVRERGVNPYNILAITFTNKAAREMKSRIGNLMGG
EAESIWISTFHSMCVRILRRDIDRIGYERNFTILDGSDQLSVIKGILKEKNVDPKKFEPRGILASISNAKNELITASEYI
KEASGFYDKMVGEVYEKYEKKLKKNQALDFDDLIMVTIQLFERVPDVLEYYQRKFQYIHVDEYQDTNHAQYLLVKQLASR
FKNLCVVGDSDQSIYGWRGADISNIMSFEKDYPNAKTILLEENYRSTKRILEAANRVIENNGNRKPKNLWTSNAEGKKIF
YHKALTEKEEAAYVVMKIQEEVNNSNRPLSDFAILYRTNAQSRVMEEYFMKSNMAYTMVGGTKFYDRKEIKDILAYLRLI
SNNEDDISLTRIVNVPKRGVGPGTLDKLNNVATTYDLSLFEVLNRIELAGISPKISKDLVAFHDLIRGFTQMQDFLSVTE
LVEEILEKTGYRAMLKNERTIEAQTRLENIDEFLSVTQNFEKENDDKTLIAFLTDLALVADVDKLEEDNEEQNGAVTLMT
LHSAKGLEFPVVFLVGMEEGIFPHSRAIYEEDEMEEERRLAYVGITRAEEELFLTSAYSRMLYGRPSSNQESRFIGEIPR
DLLELGNENKLKADKPYAKPRMPQKTTTAYKSSGAETLGWTVGDKASHKKWGVGTVVSVKGEGSGMELDIAFPSPTGVKR
LLAEFAPIEKV

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=738, Percent_Identity=41.1924119241192, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI48994965, Length=649, Percent_Identity=40.9861325115562, Blast_Score=444, Evalue=1e-125,
Organism=Escherichia coli, GI1787196, Length=339, Percent_Identity=28.9085545722714, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6322369, Length=762, Percent_Identity=29.2650918635171, Blast_Score=250, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6324477, Length=697, Percent_Identity=22.9555236728838, Blast_Score=111, Evalue=4e-25,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83183; Mature: 83183

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKELVDGLNPEQRRAVESTEGPLLIMAGAGSGKTRVLTHRIAYLVRERGVNPYNILAI
CCHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
TFTNKAAREMKSRIGNLMGGEAESIWISTFHSMCVRILRRDIDRIGYERNFTILDGSDQL
EECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHH
SVIKGILKEKNVDPKKFEPRGILASISNAKNELITASEYIKEASGFYDKMVGEVYEKYEK
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLKKNQALDFDDLIMVTIQLFERVPDVLEYYQRKFQYIHVDEYQDTNHAQYLLVKQLASR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHH
FKNLCVVGDSDQSIYGWRGADISNIMSFEKDYPNAKTILLEENYRSTKRILEAANRVIEN
HHCEEEECCCCCCEECCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHC
NGNRKPKNLWTSNAEGKKIFYHKALTEKEEAAYVVMKIQEEVNNSNRPLSDFAILYRTNA
CCCCCCCCCCCCCCCCCEEEEEHHHCCCCHHHEEEEEEHHHHCCCCCCHHHEEEEEECCC
QSRVMEEYFMKSNMAYTMVGGTKFYDRKEIKDILAYLRLISNNEDDISLTRIVNVPKRGV
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
GPGTLDKLNNVATTYDLSLFEVLNRIELAGISPKISKDLVAFHDLIRGFTQMQDFLSVTE
CCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEEILEKTGYRAMLKNERTIEAQTRLENIDEFLSVTQNFEKENDDKTLIAFLTDLALVA
HHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
DVDKLEEDNEEQNGAVTLMTLHSAKGLEFPVVFLVGMEEGIFPHSRAIYEEDEMEEERRL
HHHHHHCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCE
AYVGITRAEEELFLTSAYSRMLYGRPSSNQESRFIGEIPRDLLELGNENKLKADKPYAKP
EEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
RMPQKTTTAYKSSGAETLGWTVGDKASHKKWGVGTVVSVKGEGSGMELDIAFPSPTGVKR
CCCCHHHHHHHCCCCCEECEECCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHH
LLAEFAPIEKV
HHHHHCCCCCC
>Mature Secondary Structure
MNAKELVDGLNPEQRRAVESTEGPLLIMAGAGSGKTRVLTHRIAYLVRERGVNPYNILAI
CCHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
TFTNKAAREMKSRIGNLMGGEAESIWISTFHSMCVRILRRDIDRIGYERNFTILDGSDQL
EECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHH
SVIKGILKEKNVDPKKFEPRGILASISNAKNELITASEYIKEASGFYDKMVGEVYEKYEK
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLKKNQALDFDDLIMVTIQLFERVPDVLEYYQRKFQYIHVDEYQDTNHAQYLLVKQLASR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHH
FKNLCVVGDSDQSIYGWRGADISNIMSFEKDYPNAKTILLEENYRSTKRILEAANRVIEN
HHCEEEECCCCCCEECCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHC
NGNRKPKNLWTSNAEGKKIFYHKALTEKEEAAYVVMKIQEEVNNSNRPLSDFAILYRTNA
CCCCCCCCCCCCCCCCCEEEEEHHHCCCCHHHEEEEEEHHHHCCCCCCHHHEEEEEECCC
QSRVMEEYFMKSNMAYTMVGGTKFYDRKEIKDILAYLRLISNNEDDISLTRIVNVPKRGV
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
GPGTLDKLNNVATTYDLSLFEVLNRIELAGISPKISKDLVAFHDLIRGFTQMQDFLSVTE
CCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEEILEKTGYRAMLKNERTIEAQTRLENIDEFLSVTQNFEKENDDKTLIAFLTDLALVA
HHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
DVDKLEEDNEEQNGAVTLMTLHSAKGLEFPVVFLVGMEEGIFPHSRAIYEEDEMEEERRL
HHHHHHCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCE
AYVGITRAEEELFLTSAYSRMLYGRPSSNQESRFIGEIPRDLLELGNENKLKADKPYAKP
EEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
RMPQKTTTAYKSSGAETLGWTVGDKASHKKWGVGTVVSVKGEGSGMELDIAFPSPTGVKR
CCCCHHHHHHHCCCCCEECEECCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHH
LLAEFAPIEKV
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]