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The map label for this gene is ligA

Identifier: 226224361

GI number: 226224361

Start: 1814376

End: 1816391

Strand: Reverse

Name: ligA

Synonym: Lm4b_01772

Alternate gene names: 226224361

Gene position: 1816391-1814376 (Counterclockwise)

Preceding gene: 226224362

Following gene: 226224360

Centisome position: 62.36

GC content: 40.72

Gene sequence:

>2016_bases
ATGGCTGATAAAAAAAGGTATGAGGAACTAATCAACATACTTGATCAGTACAGCTATGATTATTATGTAATTGATAATCC
AACAGTAGAAGATGCCGAATACGATCAGAAGATGCAAGAATTACTTAAAATAGAAGAAGCGCATCCCGAGTGGGTTACAC
CTGAGTCTCCGTCAAAACGAGTTGGCGGGGAAGTTTTAGAAGGTTTTAAAAAAGTAGCACATGACACGCCGATGTTAAGT
CTTGCCAACGCTTTTAACCAAGAAGACTTGGCAGACTTTGACCGCCGAATTCGCGACAAAGTGGGCGATGATATTGCTTA
TATGTGCGAACTTAAAATTGACGGACTAGCAGTATCCCTTCAATACGAAAATGGTAAATACAAACAAGGTGCTACTCGTG
GTGATGGAACCATTGGGGAAGACATTACTGCTAACTTGCGTACGATTCGCTCCATCCCAATGAAACTTCAAAAGGACTAT
TCCATTGAAGTTCGCGGTGAAGCTTTCATGCCAAAACGCTCCTTCCAAAAACTAAATGAAATTCGCGAAGAAGAAGGCCA
GATGTTATTTGCCAATCCACGAAATGCTGCGGCTGGCTCGCTTCGACAATTGGACACAAAAATCGCAGCGTCGAGAAATC
TCGATATCTTCCTTTATGCAGTAGCAGATTTTGGTGAAATGGGCGTTGAAACTCATAGCGCAGGCTTAGATATGCTTGAA
ACACTTGGTCTTAAAGTCAATAAAGAACGCCGACTTTGCAACAGTTTAGAAGAAGTTTATGCGTACATTGAAGAATGGAC
AGAAAAACGCGCCGGATTAGCATACGATATTGACGGCATTGTTTTAAAATTAAACAACTTAGAGCAACAACGCCAAATGG
GAAACACCGTTAAATCGCCTCGTTGGTCGATTGCTTATAAATTCCCAGCCGAAGAAGTACCAACTAAGTTACTCGATATC
GAATTAAATGTTGGTAGAACTGGTGTGATAACTCCCACCGCCGTGTTAGAACCAGTGCGAGTGGCAGGGACAACCGTTAG
CCGCGCTTCGCTTCATAATGAAGACCTAATTACAGAGAAAGATATCCGCATTGGCGACACCGTTTTAATCAAAAAAGCTG
GCGATATCATTCCAGAAGTCATTAAAAGCATCACCGAAAAACGTAGCGGAAGTGAAGAACCTTTCCATATGCCAAAAAAT
TGTCCAGCGTGCGATAGTGAATTGGTTCGGTTAGAAGAAGAAGTGGCGTTAAGATGTATTAATCCTAAATGCCCAGCTCA
AATAAAAGAAGGACTTATCCACTTTGTCTCCAGAAATGCGATGAACATTGATGGCCTCGGCGAAAAAGTAATTATCCAGC
TATTTTCACAGCATTTAATTAAAGATGTAGCAGATTTGTTTTTCCTTTCGAAGGAGAAACTGTTAGAATTAGAAAGAATG
GGTGAGAAATCAGTAACTAATTTACTGGCATCCATCGAAGCAAGTAAACAAAACTCGCTTGAAAAATTACTTTTCGGCTT
AGGCATTCGCCATGTTGGTGCAAAAGCCGCCAAATCACTTGCCATTCATTTCGATACAATGGATAATTTGAAAGTAGCAG
ACAAAGAAACACTGACAAGTATTAACGACATTGGTGAAAAAATGGCAGACAGTATTGTCACTTATTTCGCGAATGAAGAA
GTACATGATTTATTAGAAGAACTAAAAAGGGCTGGCGTCAATATGACCTATACAGGGCCAAAACTAGAAGATATGTCCGA
GGAAGAACTTGTTTTCGCAGGTAAAACCGTTGTCTTAACTGGGAAACTAGAAAAGTTAACGCGGAATGATGCAAAAGCAT
TAATCGAATCCCTCGGAGGAAATGTTTCTGGAAGCGTCAGTAAGAAAACGGATGTTGTTGTAGCTGGCAGTGATGCTGGT
TCTAAATTAGCCAAAGCGGAAGAACTAGCCATTCCTATTTGGTCAGAAGAAGACTTAATAGAGTACTTACCAGACGAAGG
TGGATTAAACGAATGA

Upstream 100 bases:

>100_bases
CTAGATATTGCATTCCCAAGCCCAACTGGCGTCAAACGATTGCTCGCAGAATTTGCGCCAATTGAAAAAGTATAAATTTG
GAAAAAGCAGGTGAAGAATG

Downstream 100 bases:

>100_bases
AAAAAATCATAATAGCAGCGCTCGGCTTAACGCTTGTGCTCTCTGGCTGTGCCCCAAAACTTGACTCAAATGATAAAGTG
GTACAAAAAGATGATTCAAA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MADKKRYEELINILDQYSYDYYVIDNPTVEDAEYDQKMQELLKIEEAHPEWVTPESPSKRVGGEVLEGFKKVAHDTPMLS
LANAFNQEDLADFDRRIRDKVGDDIAYMCELKIDGLAVSLQYENGKYKQGATRGDGTIGEDITANLRTIRSIPMKLQKDY
SIEVRGEAFMPKRSFQKLNEIREEEGQMLFANPRNAAAGSLRQLDTKIAASRNLDIFLYAVADFGEMGVETHSAGLDMLE
TLGLKVNKERRLCNSLEEVYAYIEEWTEKRAGLAYDIDGIVLKLNNLEQQRQMGNTVKSPRWSIAYKFPAEEVPTKLLDI
ELNVGRTGVITPTAVLEPVRVAGTTVSRASLHNEDLITEKDIRIGDTVLIKKAGDIIPEVIKSITEKRSGSEEPFHMPKN
CPACDSELVRLEEEVALRCINPKCPAQIKEGLIHFVSRNAMNIDGLGEKVIIQLFSQHLIKDVADLFFLSKEKLLELERM
GEKSVTNLLASIEASKQNSLEKLLFGLGIRHVGAKAAKSLAIHFDTMDNLKVADKETLTSINDIGEKMADSIVTYFANEE
VHDLLEELKRAGVNMTYTGPKLEDMSEEELVFAGKTVVLTGKLEKLTRNDAKALIESLGGNVSGSVSKKTDVVVAGSDAG
SKLAKAEELAIPIWSEEDLIEYLPDEGGLNE

Sequences:

>Translated_671_residues
MADKKRYEELINILDQYSYDYYVIDNPTVEDAEYDQKMQELLKIEEAHPEWVTPESPSKRVGGEVLEGFKKVAHDTPMLS
LANAFNQEDLADFDRRIRDKVGDDIAYMCELKIDGLAVSLQYENGKYKQGATRGDGTIGEDITANLRTIRSIPMKLQKDY
SIEVRGEAFMPKRSFQKLNEIREEEGQMLFANPRNAAAGSLRQLDTKIAASRNLDIFLYAVADFGEMGVETHSAGLDMLE
TLGLKVNKERRLCNSLEEVYAYIEEWTEKRAGLAYDIDGIVLKLNNLEQQRQMGNTVKSPRWSIAYKFPAEEVPTKLLDI
ELNVGRTGVITPTAVLEPVRVAGTTVSRASLHNEDLITEKDIRIGDTVLIKKAGDIIPEVIKSITEKRSGSEEPFHMPKN
CPACDSELVRLEEEVALRCINPKCPAQIKEGLIHFVSRNAMNIDGLGEKVIIQLFSQHLIKDVADLFFLSKEKLLELERM
GEKSVTNLLASIEASKQNSLEKLLFGLGIRHVGAKAAKSLAIHFDTMDNLKVADKETLTSINDIGEKMADSIVTYFANEE
VHDLLEELKRAGVNMTYTGPKLEDMSEEELVFAGKTVVLTGKLEKLTRNDAKALIESLGGNVSGSVSKKTDVVVAGSDAG
SKLAKAEELAIPIWSEEDLIEYLPDEGGLNE
>Mature_670_residues
ADKKRYEELINILDQYSYDYYVIDNPTVEDAEYDQKMQELLKIEEAHPEWVTPESPSKRVGGEVLEGFKKVAHDTPMLSL
ANAFNQEDLADFDRRIRDKVGDDIAYMCELKIDGLAVSLQYENGKYKQGATRGDGTIGEDITANLRTIRSIPMKLQKDYS
IEVRGEAFMPKRSFQKLNEIREEEGQMLFANPRNAAAGSLRQLDTKIAASRNLDIFLYAVADFGEMGVETHSAGLDMLET
LGLKVNKERRLCNSLEEVYAYIEEWTEKRAGLAYDIDGIVLKLNNLEQQRQMGNTVKSPRWSIAYKFPAEEVPTKLLDIE
LNVGRTGVITPTAVLEPVRVAGTTVSRASLHNEDLITEKDIRIGDTVLIKKAGDIIPEVIKSITEKRSGSEEPFHMPKNC
PACDSELVRLEEEVALRCINPKCPAQIKEGLIHFVSRNAMNIDGLGEKVIIQLFSQHLIKDVADLFFLSKEKLLELERMG
EKSVTNLLASIEASKQNSLEKLLFGLGIRHVGAKAAKSLAIHFDTMDNLKVADKETLTSINDIGEKMADSIVTYFANEEV
HDLLEELKRAGVNMTYTGPKLEDMSEEELVFAGKTVVLTGKLEKLTRNDAKALIESLGGNVSGSVSKKTDVVVAGSDAGS
KLAKAEELAIPIWSEEDLIEYLPDEGGLNE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=668, Percent_Identity=46.1077844311377, Blast_Score=599, Evalue=1e-172,
Organism=Escherichia coli, GI87082305, Length=500, Percent_Identity=23.8, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74798; Mature: 74667

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50172 BRCT ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKKRYEELINILDQYSYDYYVIDNPTVEDAEYDQKMQELLKIEEAHPEWVTPESPSKR
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
VGGEVLEGFKKVAHDTPMLSLANAFNQEDLADFDRRIRDKVGDDIAYMCELKIDGLAVSL
CCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEE
QYENGKYKQGATRGDGTIGEDITANLRTIRSIPMKLQKDYSIEVRGEAFMPKRSFQKLNE
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCEEEECCCCCCCHHHHHHHHH
IREEEGQMLFANPRNAAAGSLRQLDTKIAASRNLDIFLYAVADFGEMGVETHSAGLDMLE
HHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCCHHHHHHHHHH
TLGLKVNKERRLCNSLEEVYAYIEEWTEKRAGLAYDIDGIVLKLNNLEQQRQMGNTVKSP
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCHHHHHHHCCCCCCC
RWSIAYKFPAEEVPTKLLDIELNVGRTGVITPTAVLEPVRVAGTTVSRASLHNEDLITEK
CEEEEEECCHHHHCCEEEEEEEECCCCCCCCHHHHHCCHHHHCCCHHHHHCCCCCCCCCC
DIRIGDTVLIKKAGDIIPEVIKSITEKRSGSEEPFHMPKNCPACDSELVRLEEEVALRCI
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEC
NPKCPAQIKEGLIHFVSRNAMNIDGLGEKVIIQLFSQHLIKDVADLFFLSKEKLLELERM
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEKSVTNLLASIEASKQNSLEKLLFGLGIRHVGAKAAKSLAIHFDTMDNLKVADKETLTS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEECCHHHHHH
INDIGEKMADSIVTYFANEEVHDLLEELKRAGVNMTYTGPKLEDMSEEELVFAGKTVVLT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEECCEEEEE
GKLEKLTRNDAKALIESLGGNVSGSVSKKTDVVVAGSDAGSKLAKAEELAIPIWSEEDLI
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHEECCCCCCHHH
EYLPDEGGLNE
HHCCCCCCCCC
>Mature Secondary Structure 
ADKKRYEELINILDQYSYDYYVIDNPTVEDAEYDQKMQELLKIEEAHPEWVTPESPSKR
CCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
VGGEVLEGFKKVAHDTPMLSLANAFNQEDLADFDRRIRDKVGDDIAYMCELKIDGLAVSL
CCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEE
QYENGKYKQGATRGDGTIGEDITANLRTIRSIPMKLQKDYSIEVRGEAFMPKRSFQKLNE
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCEEEECCCCCCCHHHHHHHHH
IREEEGQMLFANPRNAAAGSLRQLDTKIAASRNLDIFLYAVADFGEMGVETHSAGLDMLE
HHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCCHHHHHHHHHH
TLGLKVNKERRLCNSLEEVYAYIEEWTEKRAGLAYDIDGIVLKLNNLEQQRQMGNTVKSP
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCHHHHHHHCCCCCCC
RWSIAYKFPAEEVPTKLLDIELNVGRTGVITPTAVLEPVRVAGTTVSRASLHNEDLITEK
CEEEEEECCHHHHCCEEEEEEEECCCCCCCCHHHHHCCHHHHCCCHHHHHCCCCCCCCCC
DIRIGDTVLIKKAGDIIPEVIKSITEKRSGSEEPFHMPKNCPACDSELVRLEEEVALRCI
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEC
NPKCPAQIKEGLIHFVSRNAMNIDGLGEKVIIQLFSQHLIKDVADLFFLSKEKLLELERM
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEKSVTNLLASIEASKQNSLEKLLFGLGIRHVGAKAAKSLAIHFDTMDNLKVADKETLTS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEECCHHHHHH
INDIGEKMADSIVTYFANEEVHDLLEELKRAGVNMTYTGPKLEDMSEEELVFAGKTVVLT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEECCEEEEE
GKLEKLTRNDAKALIESLGGNVSGSVSKKTDVVVAGSDAGSKLAKAEELAIPIWSEEDLI
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHEECCCCCCHHH
EYLPDEGGLNE
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]