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The map label for this gene is comEC

Identifier: 226224083

GI number: 226224083

Start: 1499056

End: 1501278

Strand: Reverse

Name: comEC

Synonym: Lm4b_01492

Alternate gene names: 226224083

Gene position: 1501278-1499056 (Counterclockwise)

Preceding gene: 226224084

Following gene: 226224082

Centisome position: 51.54

GC content: 33.74

Gene sequence:

>2223_bases
ATTATTGTTTTAGCTATTGTTTGTGCGAATTTTACAAGCTCTATCGTTCCCATATGTATTTGTTTGCTAGGTTTAATTGC
TTTTATTAAAAAATCCAAAATTATTCTTCTTTTTGTCCTCATTTATTTTCTAACCTATAGTTTTTTGTTTGTAATAGAAA
AATTCAATACTTCCTCTTTTACAGCAACTGAATATAATGGGAACTGTCAAATTATAGATAATTTAAAAATCGATGGAGAT
AGTTTTCAAACTTTTGTACGCTGCGAAAAAGAAAAATTTCAATTGAGTTATAAAATTACAACGGAAGAGGAACAACAAAA
ACTAAAAAAGCTCCATTACGGACAGTTTATTTCTGTTTCTGCAAATATAGAAACACCTTCCGTCAACCGCAATCAAAATC
AATTCAATTACCAAACTTATTTAAAGAACCAAAATGTACACTATATACTTCGAGCAAGTAATCTTGCTATTTCCAGTCAA
TTCTCACCATCTTTCCTAATGAAACTTCAAAATATACGTTTGAAAATAATTACCCATTTAACCGAAAAAATATCTCCAAC
CATTTCTCCTTATTGTCTCGCATTAATTAGTGGAGAGAAAAACGGCTTTTCCCCCGAAATGTATGAAGTTTATCAACAAA
TGGGTGTTGTCCATTTGCTAGCTATCTCTGGACTTCACGTGAATTTATTAATAGGGGCAATATATTTTCTCCTACTAAAA
TTCGGTATTACAAGGGATAGAGCCATCACTTGTCTACTTGTTTTCTTACCATTTTACGTTATCCTTACAGGAGCAAATCC
GCCAGTCATACGAGCTGCTACAATGACAGCATTACTTTTATTGTCAGAAAAATATACTACTAAATGGAGTTCGTTTTCTG
CCATTTGTTTGTCCTTTATTCTGTTTTTCCTCTTGCAACCGTATATCATTAGAGAAGTAGGTTTTCAACTATCTTATGCA
GTATCGTTTGGAATTATTTTATCGTCAAGACAAATACTAACCAGACAGCAGAACGCATTTACTAAGTCACTTGCTATTTC
CTTCATTTCAACCATCATGTCTTCAGCTGTCATGATGTATCATTTTTACTCATTTTCATGGGTAGGGATATTCTTTAATT
TAATTTATGTGCCCATTTTTACGATGATTATTTTGCCAGGTTGTCTAACTGTGTTTGTGCTGTCTTTTTTTTCAACAGAA
ATATTTCATTTTCCTGAAGCAATGCTTACATTCTTTATACAATTGGTAGAAAAATTAACCTACATTTTCGCCAAGATTCC
GCATCAGACAATTGTAACAGGGAGGCCAAACACCGTTATATTAGTGCTAACCATTATAACTATCATCATCGTCTTCAGCC
AGTGGCAAAAGAAAAAGTTTCCTTTAGGGATATTCATTTGTTTTTGTTTATTATGTTATTTAGCTAGTTTTAATTTTAGT
GGAAAAGTTAGTTTTATCGATGTGGGGCAAGGGGATAGCATTTTAATTCAACTACCATACAATCAAGGGAATTATTTAAT
AGATACTGGAGGCCAACTTCCATTTGAAAAAGAAGCATGGGCTAAGAAAAGAAAACCATTTACAATAGGCGGAAGTACAC
TTAGCCCAGTGTTAAAATCAAAAGGAATTAATAGCTTAGATAAAGTAATTATTACACATAGCGATGCTGACCACATGGAA
GGACTTGATGATTTACAGAAAAACATTACTATTAAAGAGCTTATTTTTGCTAAAGGAGCAGAAAACAAACCCATCATGAA
AGAAGCTCTCGCTGCGATGCCAAAAGTGAAACAGACAATTATTTTGGCCGGTGCCAAGTGGCAAATTGGCGAAAACAGTT
TCGAATGCTTATATCCCGATAAAGCAGGAGAAGGAGGAAATGATGACTCTATTGTATTAAAGGCAGTATTAGATGGTAAA
ATATGGCTTTTTACTGGTGATTTAGAGGCGAACGGAGAAAAGGGCATATCAGAACAACCTATCAAAGCAGATATTTTGAA
AGTAGGGCATCATGGTAGCAAGACCTCCAGTTCCAAAGAATTTATTCAAAAAGTGAAGCCGACTTTTGCAGTTATTTCAT
GCGGGTTAAACAATCGCTTTGGTCATCCACACGCAGAAACTATTAATACAATAGAAACAGCAGGAGTAACAATTTTACGA
ACAGATGTACAAGGGGAAATAACTTATACATTTGGAAAAGGCTTTGAAGCGACTTTGAAATAA

Upstream 100 bases:

>100_bases
GAAGTAAATATCGAACCAGAAAAAGCAGAGCAACTTTATCAAAACATCTCAGAGAAATTGAACTAAATAGGGAGGGGACG
TTATCGAGCGATATTTAGCT

Downstream 100 bases:

>100_bases
AACTTGCTATCTCTTATTTCATGTTTTACCATAGAAAGAGAAATAAGAGTGAAGCGAGGCGAAATGAATGCTGCCAGAAT
GGAAACAGATACGCTCCAAA

Product: integral membrane protein ComEC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 740; Mature: 740

Protein sequence:

>740_residues
MIVLAIVCANFTSSIVPICICLLGLIAFIKKSKIILLFVLIYFLTYSFLFVIEKFNTSSFTATEYNGNCQIIDNLKIDGD
SFQTFVRCEKEKFQLSYKITTEEEQQKLKKLHYGQFISVSANIETPSVNRNQNQFNYQTYLKNQNVHYILRASNLAISSQ
FSPSFLMKLQNIRLKIITHLTEKISPTISPYCLALISGEKNGFSPEMYEVYQQMGVVHLLAISGLHVNLLIGAIYFLLLK
FGITRDRAITCLLVFLPFYVILTGANPPVIRAATMTALLLLSEKYTTKWSSFSAICLSFILFFLLQPYIIREVGFQLSYA
VSFGIILSSRQILTRQQNAFTKSLAISFISTIMSSAVMMYHFYSFSWVGIFFNLIYVPIFTMIILPGCLTVFVLSFFSTE
IFHFPEAMLTFFIQLVEKLTYIFAKIPHQTIVTGRPNTVILVLTIITIIIVFSQWQKKKFPLGIFICFCLLCYLASFNFS
GKVSFIDVGQGDSILIQLPYNQGNYLIDTGGQLPFEKEAWAKKRKPFTIGGSTLSPVLKSKGINSLDKVIITHSDADHME
GLDDLQKNITIKELIFAKGAENKPIMKEALAAMPKVKQTIILAGAKWQIGENSFECLYPDKAGEGGNDDSIVLKAVLDGK
IWLFTGDLEANGEKGISEQPIKADILKVGHHGSKTSSSKEFIQKVKPTFAVISCGLNNRFGHPHAETINTIETAGVTILR
TDVQGEITYTFGKGFEATLK

Sequences:

>Translated_740_residues
MIVLAIVCANFTSSIVPICICLLGLIAFIKKSKIILLFVLIYFLTYSFLFVIEKFNTSSFTATEYNGNCQIIDNLKIDGD
SFQTFVRCEKEKFQLSYKITTEEEQQKLKKLHYGQFISVSANIETPSVNRNQNQFNYQTYLKNQNVHYILRASNLAISSQ
FSPSFLMKLQNIRLKIITHLTEKISPTISPYCLALISGEKNGFSPEMYEVYQQMGVVHLLAISGLHVNLLIGAIYFLLLK
FGITRDRAITCLLVFLPFYVILTGANPPVIRAATMTALLLLSEKYTTKWSSFSAICLSFILFFLLQPYIIREVGFQLSYA
VSFGIILSSRQILTRQQNAFTKSLAISFISTIMSSAVMMYHFYSFSWVGIFFNLIYVPIFTMIILPGCLTVFVLSFFSTE
IFHFPEAMLTFFIQLVEKLTYIFAKIPHQTIVTGRPNTVILVLTIITIIIVFSQWQKKKFPLGIFICFCLLCYLASFNFS
GKVSFIDVGQGDSILIQLPYNQGNYLIDTGGQLPFEKEAWAKKRKPFTIGGSTLSPVLKSKGINSLDKVIITHSDADHME
GLDDLQKNITIKELIFAKGAENKPIMKEALAAMPKVKQTIILAGAKWQIGENSFECLYPDKAGEGGNDDSIVLKAVLDGK
IWLFTGDLEANGEKGISEQPIKADILKVGHHGSKTSSSKEFIQKVKPTFAVISCGLNNRFGHPHAETINTIETAGVTILR
TDVQGEITYTFGKGFEATLK
>Mature_740_residues
MIVLAIVCANFTSSIVPICICLLGLIAFIKKSKIILLFVLIYFLTYSFLFVIEKFNTSSFTATEYNGNCQIIDNLKIDGD
SFQTFVRCEKEKFQLSYKITTEEEQQKLKKLHYGQFISVSANIETPSVNRNQNQFNYQTYLKNQNVHYILRASNLAISSQ
FSPSFLMKLQNIRLKIITHLTEKISPTISPYCLALISGEKNGFSPEMYEVYQQMGVVHLLAISGLHVNLLIGAIYFLLLK
FGITRDRAITCLLVFLPFYVILTGANPPVIRAATMTALLLLSEKYTTKWSSFSAICLSFILFFLLQPYIIREVGFQLSYA
VSFGIILSSRQILTRQQNAFTKSLAISFISTIMSSAVMMYHFYSFSWVGIFFNLIYVPIFTMIILPGCLTVFVLSFFSTE
IFHFPEAMLTFFIQLVEKLTYIFAKIPHQTIVTGRPNTVILVLTIITIIIVFSQWQKKKFPLGIFICFCLLCYLASFNFS
GKVSFIDVGQGDSILIQLPYNQGNYLIDTGGQLPFEKEAWAKKRKPFTIGGSTLSPVLKSKGINSLDKVIITHSDADHME
GLDDLQKNITIKELIFAKGAENKPIMKEALAAMPKVKQTIILAGAKWQIGENSFECLYPDKAGEGGNDDSIVLKAVLDGK
IWLFTGDLEANGEKGISEQPIKADILKVGHHGSKTSSSKEFIQKVKPTFAVISCGLNNRFGHPHAETINTIETAGVTILR
TDVQGEITYTFGKGFEATLK

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG0658

COG function: function code R; Predicted membrane metal-binding protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=663, Percent_Identity=25.0377073906486, Blast_Score=106, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 83033; Mature: 83033

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLAIVCANFTSSIVPICICLLGLIAFIKKSKIILLFVLIYFLTYSFLFVIEKFNTSSF
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TATEYNGNCQIIDNLKIDGDSFQTFVRCEKEKFQLSYKITTEEEQQKLKKLHYGQFISVS
EEEEECCCEEEEEEEEECCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCEEEEE
ANIETPSVNRNQNQFNYQTYLKNQNVHYILRASNLAISSQFSPSFLMKLQNIRLKIITHL
ECCCCCCCCCCCCCCCHHHEECCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHH
TEKISPTISPYCLALISGEKNGFSPEMYEVYQQMGVVHLLAISGLHVNLLIGAIYFLLLK
HHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHH
FGITRDRAITCLLVFLPFYVILTGANPPVIRAATMTALLLLSEKYTTKWSSFSAICLSFI
HCCCCHHHHHHHHHHHHHHHEEECCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LFFLLQPYIIREVGFQLSYAVSFGIILSSRQILTRQQNAFTKSLAISFISTIMSSAVMMY
HHHHHHHHHHHHHCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFYSFSWVGIFFNLIYVPIFTMIILPGCLTVFVLSFFSTEIFHFPEAMLTFFIQLVEKLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIFAKIPHQTIVTGRPNTVILVLTIITIIIVFSQWQKKKFPLGIFICFCLLCYLASFNFS
HHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
GKVSFIDVGQGDSILIQLPYNQGNYLIDTGGQLPFEKEAWAKKRKPFTIGGSTLSPVLKS
CEEEEEECCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHHCCCCEEECCCHHHHHHHH
KGINSLDKVIITHSDADHMEGLDDLQKNITIKELIFAKGAENKPIMKEALAAMPKVKQTI
CCCCCHHEEEEECCCCHHHCCHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHCCHHCEEE
ILAGAKWQIGENSFECLYPDKAGEGGNDDSIVLKAVLDGKIWLFTGDLEANGEKGISEQP
EEECCEEEECCCCCEEECCCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCC
IKADILKVGHHGSKTSSSKEFIQKVKPTFAVISCGLNNRFGHPHAETINTIETAGVTILR
CHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEE
TDVQGEITYTFGKGFEATLK
ECCCCEEEEEECCCEEECCC
>Mature Secondary Structure
MIVLAIVCANFTSSIVPICICLLGLIAFIKKSKIILLFVLIYFLTYSFLFVIEKFNTSSF
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TATEYNGNCQIIDNLKIDGDSFQTFVRCEKEKFQLSYKITTEEEQQKLKKLHYGQFISVS
EEEEECCCEEEEEEEEECCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCEEEEE
ANIETPSVNRNQNQFNYQTYLKNQNVHYILRASNLAISSQFSPSFLMKLQNIRLKIITHL
ECCCCCCCCCCCCCCCHHHEECCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHH
TEKISPTISPYCLALISGEKNGFSPEMYEVYQQMGVVHLLAISGLHVNLLIGAIYFLLLK
HHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHH
FGITRDRAITCLLVFLPFYVILTGANPPVIRAATMTALLLLSEKYTTKWSSFSAICLSFI
HCCCCHHHHHHHHHHHHHHHEEECCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LFFLLQPYIIREVGFQLSYAVSFGIILSSRQILTRQQNAFTKSLAISFISTIMSSAVMMY
HHHHHHHHHHHHHCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFYSFSWVGIFFNLIYVPIFTMIILPGCLTVFVLSFFSTEIFHFPEAMLTFFIQLVEKLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIFAKIPHQTIVTGRPNTVILVLTIITIIIVFSQWQKKKFPLGIFICFCLLCYLASFNFS
HHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
GKVSFIDVGQGDSILIQLPYNQGNYLIDTGGQLPFEKEAWAKKRKPFTIGGSTLSPVLKS
CEEEEEECCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHHCCCCEEECCCHHHHHHHH
KGINSLDKVIITHSDADHMEGLDDLQKNITIKELIFAKGAENKPIMKEALAAMPKVKQTI
CCCCCHHEEEEECCCCHHHCCHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHCCHHCEEE
ILAGAKWQIGENSFECLYPDKAGEGGNDDSIVLKAVLDGKIWLFTGDLEANGEKGISEQP
EEECCEEEECCCCCEEECCCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCC
IKADILKVGHHGSKTSSSKEFIQKVKPTFAVISCGLNNRFGHPHAETINTIETAGVTILR
CHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEE
TDVQGEITYTFGKGFEATLK
ECCCCEEEEEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]