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The map label for this gene is rph

Identifier: 226223838

GI number: 226223838

Start: 1245312

End: 1246058

Strand: Direct

Name: rph

Synonym: Lm4b_01243

Alternate gene names: 226223838

Gene position: 1245312-1246058 (Clockwise)

Preceding gene: 226223837

Following gene: 226223839

Centisome position: 42.75

GC content: 42.44

Gene sequence:

>747_bases
ATGAGAGTTGATGGAAGAGAAAGTAATGCATTACGAAATATTGAAGTAACACCAGATTATTTAATGCATCCAGAAGGTTC
TGTGCTAATTGCATCAGGAAATACAAAAGTTATCTGTTCTGCAAGTGTAGAAACAAAAGTGCCACCATTTATGCGAGGAG
AAGGCCGCGGCTGGATTTCTGCAGAATACTCGATGTTACCACGAGCAACAAACACCCGAAATATCCGTGAATCTTCAAAA
GGTAAAGTTACTGGCAGAACAATGGAAATTCAACGTTTAATCGGACGTGCGCTCCGTGCAGTTGTTGATTTAGATGCCCT
TGGTGAAAGAACAATTTGGCTCGATTGTGACGTTATCCAAGCAGATGGAGGTACTCGTACAGCTTCTATCACCGGCGCAT
TCATCGCGATGGTCATGGCAATCGCCAAACTAGACGAAGCTGTACCATTTGCTAAATTTCCTGTCAAAGATTTTCTAGCA
GCAACTAGCGTAGGTGTTTTAGAAGAGGGTGGAACAGTACTTGACCTTAACTACGTAGAAGACAGCGCAGCGCAAGTAGA
TATGAATATTATCATGACTGGTAGCGGCGCCTTTGTAGAACTCCAAGGAACTGGCGAAGAAGCTACATTCTCGGAAACAG
AACTGGCTGAACTAATTGCCTTAGGTAAAAAAGGAATTAGCGAATTAATCGAAATCCAAAAAGAAACACTCGGAGACAAA
GTCACAGCGCGGATTAAAGGAGAATAA

Upstream 100 bases:

>100_bases
GGGCTCGACACAAATTTTCAAAGATATTGCAAAAGACTGGTTAAATATGCCAGATATGACTGTAGAACATATTAAATTAG
GAAAATAAGAGGTGCCAATA

Downstream 100 bases:

>100_bases
AAAATGAGTAAAATTATTATCGCTACCGCAAACAAAGGAAAAGCAAAAGAATTCGAAAAAATCTTTGCGAAATTTAATAT
CGAAGTAGCAACCCTTGCTG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRVDGRESNALRNIEVTPDYLMHPEGSVLIASGNTKVICSASVETKVPPFMRGEGRGWISAEYSMLPRATNTRNIRESSK
GKVTGRTMEIQRLIGRALRAVVDLDALGERTIWLDCDVIQADGGTRTASITGAFIAMVMAIAKLDEAVPFAKFPVKDFLA
ATSVGVLEEGGTVLDLNYVEDSAAQVDMNIIMTGSGAFVELQGTGEEATFSETELAELIALGKKGISELIEIQKETLGDK
VTARIKGE

Sequences:

>Translated_248_residues
MRVDGRESNALRNIEVTPDYLMHPEGSVLIASGNTKVICSASVETKVPPFMRGEGRGWISAEYSMLPRATNTRNIRESSK
GKVTGRTMEIQRLIGRALRAVVDLDALGERTIWLDCDVIQADGGTRTASITGAFIAMVMAIAKLDEAVPFAKFPVKDFLA
ATSVGVLEEGGTVLDLNYVEDSAAQVDMNIIMTGSGAFVELQGTGEEATFSETELAELIALGKKGISELIEIQKETLGDK
VTARIKGE
>Mature_248_residues
MRVDGRESNALRNIEVTPDYLMHPEGSVLIASGNTKVICSASVETKVPPFMRGEGRGWISAEYSMLPRATNTRNIRESSK
GKVTGRTMEIQRLIGRALRAVVDLDALGERTIWLDCDVIQADGGTRTASITGAFIAMVMAIAKLDEAVPFAKFPVKDFLA
ATSVGVLEEGGTVLDLNYVEDSAAQVDMNIIMTGSGAFVELQGTGEEATFSETELAELIALGKKGISELIEIQKETLGDK
VTARIKGE

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=212, Percent_Identity=58.9622641509434, Blast_Score=251, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI71981632, Length=187, Percent_Identity=27.807486631016, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 26670; Mature: 26670

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDGRESNALRNIEVTPDYLMHPEGSVLIASGNTKVICSASVETKVPPFMRGEGRGWIS
CCCCCCCCCCEEEEEECCHHEECCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCEEEE
AEYSMLPRATNTRNIRESSKGKVTGRTMEIQRLIGRALRAVVDLDALGERTIWLDCDVIQ
ECCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGAFIAMVMAIAKLDEAVPFAKFPVKDFLAATSVGVLEEGGTVLDLNYVE
CCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEECCCCEEEEEEECC
DSAAQVDMNIIMTGSGAFVELQGTGEEATFSETELAELIALGKKGISELIEIQKETLGDK
CCCCEEEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VTARIKGE
EEEEEECC
>Mature Secondary Structure
MRVDGRESNALRNIEVTPDYLMHPEGSVLIASGNTKVICSASVETKVPPFMRGEGRGWIS
CCCCCCCCCCEEEEEECCHHEECCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCEEEE
AEYSMLPRATNTRNIRESSKGKVTGRTMEIQRLIGRALRAVVDLDALGERTIWLDCDVIQ
ECCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGAFIAMVMAIAKLDEAVPFAKFPVKDFLAATSVGVLEEGGTVLDLNYVE
CCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEECCCCEEEEEEECC
DSAAQVDMNIIMTGSGAFVELQGTGEEATFSETELAELIALGKKGISELIEIQKETLGDK
CCCCEEEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VTARIKGE
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]