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The map label for this gene is pheT

Identifier: 226223822

GI number: 226223822

Start: 1222782

End: 1225190

Strand: Direct

Name: pheT

Synonym: Lm4b_01227

Alternate gene names: 226223822

Gene position: 1222782-1225190 (Clockwise)

Preceding gene: 226223821

Following gene: 226223823

Centisome position: 41.98

GC content: 40.85

Gene sequence:

>2409_bases
ATGTTAGTTTCATATAATTGGGTCAAAGAATTTTTCCAAGACTTCCCGTTAACGGCGGAAGAGCTAGGGGAAGCAATTAC
AAGAACAGGTATTGAAATCGAAGGCGTAGAAGAATTAAGCGCTAGTTTGAAAAATGTCGTAGTCGGCGAAGTTTTATCAT
GCGAACGCCACCCCGATGCAGAGAAATTGAATAAATGTCTCGTTCAAACAGACGAGGTAGAACCTGTTCAAATCATTTGC
GGAGCTCCAAACGTTGCCGCTGGTCAAAAAGTAATCGTAGCCAAAGTTGGCGCAAGACTTCCAGGTGGACTTAAAATCAA
ACGCGCAAAACTACGCGGCGAAGTGTCAGAGGGAATGATTTGCTCCCTGGCTGAACTTGGCTTTGAAAGCAAAGTTGTAC
CAAAAGCATACGCAGAAGGAATTTATGTATTACCTGCGCACGTGGAAACGGGTGTAAGTGCAATCACTTTACTTGGACTA
GATGATGCGATTTTGGATATGGCAATCACACCAAACCGCGCAGATGCATTGAGCATGAATGGTGTAGCTCACGAGGTTGG
TGCGATTATTCATCAAAAACCAGCACAACCAACCGAGCCTGACGTATCTGAAAAAGGAAAAGCAGACGATTTCATTTCTG
TAGAAGTTGAAAATCCAGCCGAAACACCTTACTATGCTATTAAAATGGTAGAAAACATTGAAATCAAAGAATCGCCACTA
TGGCTGCAAACAAAACTAATGAAAGCTGGCATTCGTCCACATAATAACGTGGTCGATGTTACCAACTACATCAATTTATT
ATACGGACAACCTTTGCATTCCTTTGACTATGATAAAATTGGTAGCAAGAAAATCGTTGTACGTTCTGCAAAAGAACAAG
AAGAGATTACCACGCTTGATGGCGAGAAAAGAACTTTACAGGCTGGACATACAGTTATTACAAATGGCGTAGAACCAATT
GCTATCGCTGGCGTTATGGGTGGAGAATTTTCCGAAGTAACAGAGAACACAACGACAGTAGCGCTAGAAGGTGCTATTTT
CAGCAGCTCTTCCATCGGTAAAGCATCTCGCGAATTATACCTACGTACAGAAGCAAGCATCCGTTACGACAAAGGTTCTG
ACGCTTGGAAAGTAGAAAAAGCACTTGCACATGGCGGAGCGTTAATCGCTGAACTAAGTGGCGGAACACTAGTAGGCGGT
GTCGTAGAAGTAGATAACCGTGAAAAAGCTGTAAATAAAATCGAAACAAGCCTAACACGCATTAACCGCATTTTAGGAAC
AGCAATTACGCTTGCGGAAATCGAAACAATTTTTGATCGTTTAGGTTTTGTATTAGAAGTGAAAAATGATACTTTAATCA
TTGAAGTGCCGACAAGACGTTGGGACATTACTATTGAAGCAGATATTTTAGAAGAAGTAGCTCGTATTTACGGTTACGAT
GAAATTCCGGTAACACTTCCTGCGACAAGCACAACAGGCGGCTTATCAGACAGCCAAAAAGCTCGTCGTGTCATGCGCGC
TTATTTAGAAGGAGCAGGACTTAACCAAGCATTAACTTACTCTTTAACATCAAAAAAAGATGCAACTCGTCTAGCGCTTT
CTGATGAAAAAACAGTTGCATTATCGATGCCAATGAGTGAAGAACATAGCCATTTAAGAACAAGTATCGTTCCACAATTA
ATTCGTAGCGCAAGTTATAACATCGCTCGTAAAAATATGGACGTGGCACTTTACGAAATGGGTACCGTGTTCTACGCAAC
AGAAGGGGACAATTTACCAATTGAACAAGAGCATTTGGCAGGTCTAATTACTGGGAACTGGCATACGGTTGATTGGCAAA
AAACACCAAAACCAGTAGATTTCTTCGTTTTAAAAGGAATTGTCGAAGGTTTAGTGAACAAATTAGGTATCAAATCCGAA
CTTCACTGGAAACAAACAGAAAAAGAAGAACTGCATCCGGGAAGAACCGCCAGCCTTATTTTAGAAGGACAAGAAATTGG
TTATCTTGGAGCACTTCACCCAGCGGTAGAAGCTAACTATGATTTAAAAGAAACATATGTCTTTGAAATCAATGTAGCTG
CTTTACTAGATGCAACCAAAGAAAAAGTTGTGTACCATCCAATCCCACGTTACCCAGAAATGACTCGCGACTTAGCATTG
CTTGTGGATAAAAATACCGACCATGCAACAATTTCTCAAGTAATCCAAGAGCACGGCGGAAAGTTACTTGTCGATATCGA
GCTATTTGATATTTTCGAAGGGGAGAGTCTTGGCGAAAATAAAAAATCGCTCGCATACACATTGACTTTCCTAGATAGCG
AAAGAACACTAGTCGAAGAAGATGTACAAAAAGCAACCAATAAAGTAATAGAAGCATTACAAGAAAAACTACATGCGATT
ATTCGCTAA

Upstream 100 bases:

>100_bases
TTGCGATGTTGAAATATGCGGTGGATGATATTCGCCACCTTTATACAAATGATTTACGCTTTACGAAGCAATTCCAAAGT
ACAGAAACGGGGGAAATCTA

Downstream 100 bases:

>100_bases
ATGAAGAAGAACTAAGCTAGCTGGAAAACATTTTCAACTAGTTTGGTTCTTTTTTTGTTTTTTATAAAATAGTGGAAGTC
TTTCCTTTTTTAGTACTTCA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 802; Mature: 802

Protein sequence:

>802_residues
MLVSYNWVKEFFQDFPLTAEELGEAITRTGIEIEGVEELSASLKNVVVGEVLSCERHPDAEKLNKCLVQTDEVEPVQIIC
GAPNVAAGQKVIVAKVGARLPGGLKIKRAKLRGEVSEGMICSLAELGFESKVVPKAYAEGIYVLPAHVETGVSAITLLGL
DDAILDMAITPNRADALSMNGVAHEVGAIIHQKPAQPTEPDVSEKGKADDFISVEVENPAETPYYAIKMVENIEIKESPL
WLQTKLMKAGIRPHNNVVDVTNYINLLYGQPLHSFDYDKIGSKKIVVRSAKEQEEITTLDGEKRTLQAGHTVITNGVEPI
AIAGVMGGEFSEVTENTTTVALEGAIFSSSSIGKASRELYLRTEASIRYDKGSDAWKVEKALAHGGALIAELSGGTLVGG
VVEVDNREKAVNKIETSLTRINRILGTAITLAEIETIFDRLGFVLEVKNDTLIIEVPTRRWDITIEADILEEVARIYGYD
EIPVTLPATSTTGGLSDSQKARRVMRAYLEGAGLNQALTYSLTSKKDATRLALSDEKTVALSMPMSEEHSHLRTSIVPQL
IRSASYNIARKNMDVALYEMGTVFYATEGDNLPIEQEHLAGLITGNWHTVDWQKTPKPVDFFVLKGIVEGLVNKLGIKSE
LHWKQTEKEELHPGRTASLILEGQEIGYLGALHPAVEANYDLKETYVFEINVAALLDATKEKVVYHPIPRYPEMTRDLAL
LVDKNTDHATISQVIQEHGGKLLVDIELFDIFEGESLGENKKSLAYTLTFLDSERTLVEEDVQKATNKVIEALQEKLHAI
IR

Sequences:

>Translated_802_residues
MLVSYNWVKEFFQDFPLTAEELGEAITRTGIEIEGVEELSASLKNVVVGEVLSCERHPDAEKLNKCLVQTDEVEPVQIIC
GAPNVAAGQKVIVAKVGARLPGGLKIKRAKLRGEVSEGMICSLAELGFESKVVPKAYAEGIYVLPAHVETGVSAITLLGL
DDAILDMAITPNRADALSMNGVAHEVGAIIHQKPAQPTEPDVSEKGKADDFISVEVENPAETPYYAIKMVENIEIKESPL
WLQTKLMKAGIRPHNNVVDVTNYINLLYGQPLHSFDYDKIGSKKIVVRSAKEQEEITTLDGEKRTLQAGHTVITNGVEPI
AIAGVMGGEFSEVTENTTTVALEGAIFSSSSIGKASRELYLRTEASIRYDKGSDAWKVEKALAHGGALIAELSGGTLVGG
VVEVDNREKAVNKIETSLTRINRILGTAITLAEIETIFDRLGFVLEVKNDTLIIEVPTRRWDITIEADILEEVARIYGYD
EIPVTLPATSTTGGLSDSQKARRVMRAYLEGAGLNQALTYSLTSKKDATRLALSDEKTVALSMPMSEEHSHLRTSIVPQL
IRSASYNIARKNMDVALYEMGTVFYATEGDNLPIEQEHLAGLITGNWHTVDWQKTPKPVDFFVLKGIVEGLVNKLGIKSE
LHWKQTEKEELHPGRTASLILEGQEIGYLGALHPAVEANYDLKETYVFEINVAALLDATKEKVVYHPIPRYPEMTRDLAL
LVDKNTDHATISQVIQEHGGKLLVDIELFDIFEGESLGENKKSLAYTLTFLDSERTLVEEDVQKATNKVIEALQEKLHAI
IR
>Mature_802_residues
MLVSYNWVKEFFQDFPLTAEELGEAITRTGIEIEGVEELSASLKNVVVGEVLSCERHPDAEKLNKCLVQTDEVEPVQIIC
GAPNVAAGQKVIVAKVGARLPGGLKIKRAKLRGEVSEGMICSLAELGFESKVVPKAYAEGIYVLPAHVETGVSAITLLGL
DDAILDMAITPNRADALSMNGVAHEVGAIIHQKPAQPTEPDVSEKGKADDFISVEVENPAETPYYAIKMVENIEIKESPL
WLQTKLMKAGIRPHNNVVDVTNYINLLYGQPLHSFDYDKIGSKKIVVRSAKEQEEITTLDGEKRTLQAGHTVITNGVEPI
AIAGVMGGEFSEVTENTTTVALEGAIFSSSSIGKASRELYLRTEASIRYDKGSDAWKVEKALAHGGALIAELSGGTLVGG
VVEVDNREKAVNKIETSLTRINRILGTAITLAEIETIFDRLGFVLEVKNDTLIIEVPTRRWDITIEADILEEVARIYGYD
EIPVTLPATSTTGGLSDSQKARRVMRAYLEGAGLNQALTYSLTSKKDATRLALSDEKTVALSMPMSEEHSHLRTSIVPQL
IRSASYNIARKNMDVALYEMGTVFYATEGDNLPIEQEHLAGLITGNWHTVDWQKTPKPVDFFVLKGIVEGLVNKLGIKSE
LHWKQTEKEELHPGRTASLILEGQEIGYLGALHPAVEANYDLKETYVFEINVAALLDATKEKVVYHPIPRYPEMTRDLAL
LVDKNTDHATISQVIQEHGGKLLVDIELFDIFEGESLGENKKSLAYTLTFLDSERTLVEEDVQKATNKVIEALQEKLHAI
IR

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI124028525, Length=288, Percent_Identity=28.4722222222222, Blast_Score=101, Evalue=4e-21,
Organism=Escherichia coli, GI1788006, Length=809, Percent_Identity=35.9703337453646, Blast_Score=445, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17533403, Length=579, Percent_Identity=23.4887737478411, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355211, Length=555, Percent_Identity=21.6216216216216, Blast_Score=100, Evalue=7e-21,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 88082; Mature: 88082

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVSYNWVKEFFQDFPLTAEELGEAITRTGIEIEGVEELSASLKNVVVGEVLSCERHPDA
CEECHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCH
EKLNKCLVQTDEVEPVQIICGAPNVAAGQKVIVAKVGARLPGGLKIKRAKLRGEVSEGMI
HHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCEEEHHHHCCCCCCCHH
CSLAELGFESKVVPKAYAEGIYVLPAHVETGVSAITLLGLDDAILDMAITPNRADALSMN
HHHHHCCCCCCCCCHHHHCCEEEEECCHHCCCCEEEEECCCHHHHEEEECCCCCCCEECC
GVAHEVGAIIHQKPAQPTEPDVSEKGKADDFISVEVENPAETPYYAIKMVENIEIKESPL
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCEECCCCC
WLQTKLMKAGIRPHNNVVDVTNYINLLYGQPLHSFDYDKIGSKKIVVRSAKEQEEITTLD
HHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCCC
GEKRTLQAGHTVITNGVEPIAIAGVMGGEFSEVTENTTTVALEGAIFSSSSIGKASRELY
CCCHHHHCCCEEEECCCCCEEEEEECCCCHHHHCCCCEEEEEEEEEECCCCCCCCCCEEE
LRTEASIRYDKGSDAWKVEKALAHGGALIAELSGGTLVGGVVEVDNREKAVNKIETSLTR
EEECCEEEECCCCCHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCHHHHHHHHHHHHHH
INRILGTAITLAEIETIFDRLGFVLEVKNDTLIIEVPTRRWDITIEADILEEVARIYGYD
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCEEEEEEEHHHHHHHHHHCCCC
EIPVTLPATSTTGGLSDSQKARRVMRAYLEGAGLNQALTYSLTSKKDATRLALSDEKTVA
CCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCEEE
LSMPMSEEHSHLRTSIVPQLIRSASYNIARKNMDVALYEMGTVFYATEGDNLPIEQEHLA
EECCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECEEEEEECCCCCCCCHHHCE
GLITGNWHTVDWQKTPKPVDFFVLKGIVEGLVNKLGIKSELHWKQTEKEELHPGRTASLI
EEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEE
LEGQEIGYLGALHPAVEANYDLKETYVFEINVAALLDATKEKVVYHPIPRYPEMTRDLAL
EECCCCCEEECCCCHHCCCCCCCEEEEEEEEHHHHHHHHHCCEEECCCCCCCCHHCCEEE
LVDKNTDHATISQVIQEHGGKLLVDIELFDIFEGESLGENKKSLAYTLTFLDSERTLVEE
EEECCCCHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHH
DVQKATNKVIEALQEKLHAIIR
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLVSYNWVKEFFQDFPLTAEELGEAITRTGIEIEGVEELSASLKNVVVGEVLSCERHPDA
CEECHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCH
EKLNKCLVQTDEVEPVQIICGAPNVAAGQKVIVAKVGARLPGGLKIKRAKLRGEVSEGMI
HHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCEEEHHHHCCCCCCCHH
CSLAELGFESKVVPKAYAEGIYVLPAHVETGVSAITLLGLDDAILDMAITPNRADALSMN
HHHHHCCCCCCCCCHHHHCCEEEEECCHHCCCCEEEEECCCHHHHEEEECCCCCCCEECC
GVAHEVGAIIHQKPAQPTEPDVSEKGKADDFISVEVENPAETPYYAIKMVENIEIKESPL
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCEECCCCC
WLQTKLMKAGIRPHNNVVDVTNYINLLYGQPLHSFDYDKIGSKKIVVRSAKEQEEITTLD
HHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCCC
GEKRTLQAGHTVITNGVEPIAIAGVMGGEFSEVTENTTTVALEGAIFSSSSIGKASRELY
CCCHHHHCCCEEEECCCCCEEEEEECCCCHHHHCCCCEEEEEEEEEECCCCCCCCCCEEE
LRTEASIRYDKGSDAWKVEKALAHGGALIAELSGGTLVGGVVEVDNREKAVNKIETSLTR
EEECCEEEECCCCCHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCHHHHHHHHHHHHHH
INRILGTAITLAEIETIFDRLGFVLEVKNDTLIIEVPTRRWDITIEADILEEVARIYGYD
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCEEEEEEEHHHHHHHHHHCCCC
EIPVTLPATSTTGGLSDSQKARRVMRAYLEGAGLNQALTYSLTSKKDATRLALSDEKTVA
CCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCEEE
LSMPMSEEHSHLRTSIVPQLIRSASYNIARKNMDVALYEMGTVFYATEGDNLPIEQEHLA
EECCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECEEEEEECCCCCCCCHHHCE
GLITGNWHTVDWQKTPKPVDFFVLKGIVEGLVNKLGIKSELHWKQTEKEELHPGRTASLI
EEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEE
LEGQEIGYLGALHPAVEANYDLKETYVFEINVAALLDATKEKVVYHPIPRYPEMTRDLAL
EECCCCCEEECCCCHHCCCCCCCEEEEEEEEHHHHHHHHHCCEEECCCCCCCCHHCCEEE
LVDKNTDHATISQVIQEHGGKLLVDIELFDIFEGESLGENKKSLAYTLTFLDSERTLVEE
EEECCCCHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHH
DVQKATNKVIEALQEKLHAIIR
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]