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The map label for this gene is pheS

Identifier: 226223821

GI number: 226223821

Start: 1221730

End: 1222782

Strand: Direct

Name: pheS

Synonym: Lm4b_01226

Alternate gene names: 226223821

Gene position: 1221730-1222782 (Clockwise)

Preceding gene: 226223820

Following gene: 226223822

Centisome position: 41.95

GC content: 39.98

Gene sequence:

>1053_bases
ATGTTAGAACAGTTACAGACGCTGAAAAATGAAGCAGAAACGCAAATTAACGAAGCATCAGACTTAAAAACATTAAACGA
CTTACGGGTAAAATACCTTGGTAAAAAAGGCCCGATGACCGAAATCATGAAACAAATGGGGAAACTTTCCGCAGAAGAAA
GACCAAAAATGGGTTCACTTGCGAATGAAGTAAGAACCGCATTAACAGAAGCAATTTCAAGCAAACAACAAATTTTAGAA
ACAGAAGCAATCAATGAAAAACTAAAATCAGAAACCATTGACGTTACATTACCAGGTACTGCTCCGAGTATTGGAACAAA
ACACTTGCTAACACAAGTAATTGAAGAAATGGAAGATATGTTCATTGGGATGGGTTACGAAATTGCAGAAGGGCCAGAAG
TAGAACTAGATTACTACAACTTCGAAGCGCTAAATTTACCAAAAGATCACCCAGCTCGTGATATGCAAGATAGCTTCTAT
ATTACAGAAAATACTTTACTACGTACCCAAACTTCACCTGTACAAGCAAGAACAATGGAAAAACATGACTTCTCTAAAGG
ACCAATCAAAGTTATCTGTCCAGGGAAAGTGTACCGCCGCGACAATGATGATGCAACTCACTCCCACCAATTTACGCAAA
TTGAAGGGCTGGTTGTTGGCGAAAACATCACGTTTGCTGACTTAAAAGGAACATTAACTGTTCTCGCAAAAACAATGTTC
GGTGAAGAACGTGAAATTCGTCTTCGTCCATCCTTCTTCCCATTCACAGAACCATCCGTTGAAATGGATATCTCTTGTTT
CAAATGTGGCGGTAAAGGGTGCCGCGTTTGTAAAGGAACAGGTTGGATTGAAATTTTAGGAAGCGGAATGGTACATCCAA
ACGTGCTTGAAATGTCGAGAATTGATTCCACGCGTTACAGCGGTTTTGCTTTCGGCTTAGGACCTGAACGGGTTGCGATG
TTGAAATATGCGGTGGATGATATTCGCCACCTTTATACAAATGATTTACGCTTTACGAAGCAATTCCAAAGTACAGAAAC
GGGGGAAATCTAA

Upstream 100 bases:

>100_bases
GGGAACAATTTTTTGTTCTTATTAGGGTGGTACCGCGAGAAGTCTCGTCCCTTTGTGGATAGAGCTTTTTTATTTTGTCT
AAAAAGAAGGAGTGAACGGT

Downstream 100 bases:

>100_bases
TGTTAGTTTCATATAATTGGGTCAAAGAATTTTTCCAAGACTTCCCGTTAACGGCGGAAGAGCTAGGGGAAGCAATTACA
AGAACAGGTATTGAAATCGA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MLEQLQTLKNEAETQINEASDLKTLNDLRVKYLGKKGPMTEIMKQMGKLSAEERPKMGSLANEVRTALTEAISSKQQILE
TEAINEKLKSETIDVTLPGTAPSIGTKHLLTQVIEEMEDMFIGMGYEIAEGPEVELDYYNFEALNLPKDHPARDMQDSFY
ITENTLLRTQTSPVQARTMEKHDFSKGPIKVICPGKVYRRDNDDATHSHQFTQIEGLVVGENITFADLKGTLTVLAKTMF
GEEREIRLRPSFFPFTEPSVEMDISCFKCGGKGCRVCKGTGWIEILGSGMVHPNVLEMSRIDSTRYSGFAFGLGPERVAM
LKYAVDDIRHLYTNDLRFTKQFQSTETGEI

Sequences:

>Translated_350_residues
MLEQLQTLKNEAETQINEASDLKTLNDLRVKYLGKKGPMTEIMKQMGKLSAEERPKMGSLANEVRTALTEAISSKQQILE
TEAINEKLKSETIDVTLPGTAPSIGTKHLLTQVIEEMEDMFIGMGYEIAEGPEVELDYYNFEALNLPKDHPARDMQDSFY
ITENTLLRTQTSPVQARTMEKHDFSKGPIKVICPGKVYRRDNDDATHSHQFTQIEGLVVGENITFADLKGTLTVLAKTMF
GEEREIRLRPSFFPFTEPSVEMDISCFKCGGKGCRVCKGTGWIEILGSGMVHPNVLEMSRIDSTRYSGFAFGLGPERVAM
LKYAVDDIRHLYTNDLRFTKQFQSTETGEI
>Mature_350_residues
MLEQLQTLKNEAETQINEASDLKTLNDLRVKYLGKKGPMTEIMKQMGKLSAEERPKMGSLANEVRTALTEAISSKQQILE
TEAINEKLKSETIDVTLPGTAPSIGTKHLLTQVIEEMEDMFIGMGYEIAEGPEVELDYYNFEALNLPKDHPARDMQDSFY
ITENTLLRTQTSPVQARTMEKHDFSKGPIKVICPGKVYRRDNDDATHSHQFTQIEGLVVGENITFADLKGTLTVLAKTMF
GEEREIRLRPSFFPFTEPSVEMDISCFKCGGKGCRVCKGTGWIEILGSGMVHPNVLEMSRIDSTRYSGFAFGLGPERVAM
LKYAVDDIRHLYTNDLRFTKQFQSTETGEI

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=243, Percent_Identity=32.9218106995885, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI4758340, Length=282, Percent_Identity=29.0780141843972, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI1788007, Length=342, Percent_Identity=46.4912280701754, Blast_Score=319, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI32563657, Length=278, Percent_Identity=30.2158273381295, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17508957, Length=278, Percent_Identity=30.2158273381295, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32566635, Length=259, Percent_Identity=30.8880308880309, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=371, Percent_Identity=29.3800539083558, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=91, Percent_Identity=40.6593406593407, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI18858079, Length=313, Percent_Identity=28.4345047923323, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17137424, Length=251, Percent_Identity=29.4820717131474, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39400; Mature: 39400

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQLQTLKNEAETQINEASDLKTLNDLRVKYLGKKGPMTEIMKQMGKLSAEERPKMGSL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHH
ANEVRTALTEAISSKQQILETEAINEKLKSETIDVTLPGTAPSIGTKHLLTQVIEEMEDM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
FIGMGYEIAEGPEVELDYYNFEALNLPKDHPARDMQDSFYITENTLLRTQTSPVQARTME
HHCCCCEECCCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCEEEEECCCCCHHHHHH
KHDFSKGPIKVICPGKVYRRDNDDATHSHQFTQIEGLVVGENITFADLKGTLTVLAKTMF
HCCCCCCCEEEEECCHHEECCCCCCCCCCCEEEEEEEEEECCEEEEHHHHHHHHHHHHHC
GEEREIRLRPSFFPFTEPSVEMDISCFKCGGKGCRVCKGTGWIEILGSGMVHPNVLEMSR
CCCCEEEECCCCCCCCCCCCEEECEEEEECCCCCEEECCCCEEEEECCCCCCCCHHHHHH
IDSTRYSGFAFGLGPERVAMLKYAVDDIRHLYTNDLRFTKQFQSTETGEI
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLEQLQTLKNEAETQINEASDLKTLNDLRVKYLGKKGPMTEIMKQMGKLSAEERPKMGSL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHH
ANEVRTALTEAISSKQQILETEAINEKLKSETIDVTLPGTAPSIGTKHLLTQVIEEMEDM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
FIGMGYEIAEGPEVELDYYNFEALNLPKDHPARDMQDSFYITENTLLRTQTSPVQARTME
HHCCCCEECCCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCEEEEECCCCCHHHHHH
KHDFSKGPIKVICPGKVYRRDNDDATHSHQFTQIEGLVVGENITFADLKGTLTVLAKTMF
HCCCCCCCEEEEECCHHEECCCCCCCCCCCEEEEEEEEEECCEEEEHHHHHHHHHHHHHC
GEEREIRLRPSFFPFTEPSVEMDISCFKCGGKGCRVCKGTGWIEILGSGMVHPNVLEMSR
CCCCEEEECCCCCCCCCCCCEEECEEEEECCCCCEEECCCCEEEEECCCCCCCCHHHHHH
IDSTRYSGFAFGLGPERVAMLKYAVDDIRHLYTNDLRFTKQFQSTETGEI
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]