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The map label for this gene is cbiK

Identifier: 226223802

GI number: 226223802

Start: 1207459

End: 1208244

Strand: Direct

Name: cbiK

Synonym: Lm4b_01206

Alternate gene names: 226223802

Gene position: 1207459-1208244 (Clockwise)

Preceding gene: 226223801

Following gene: 226223803

Centisome position: 41.46

GC content: 39.95

Gene sequence:

>786_bases
ATGAAAAAAGCGATTTTAGTCGTTAGTTTTGGCACAAGTTATCCTGAAACAAGAGAAAAAACGATTGAAGCCTGTGAAAA
AAAAGTGGCCCAAGAGTTCCCAGATTACAACGTGTTTCGTGCATTCACATCCAATAAAATCATCAAAAAACTAAAAACTC
GTGACAATATGCATATTAACACACCAAGCCAAGCATTAAACCAACTAAAGGAATTAGGCTACAAAGAAGTCATTATTCAG
TCGCTACATATTATTAGTGGTGGTGAATTTGAAAAAATCACTGCACAGGTGGAAAAATTCAAGCCGGATTTCGATTCCAT
TATTGTCAGCCAACCGTTACTTGATTCGATGGAAGATTACAAAAAAGCAATCGAAGCCATTCGCCACCAAATGCCACCTT
TAAAAGAACAAGAAGCGCTAATTCTCATGGGACATGGTTCGAAGCATCGTGCTTTCAGCGCCTATGCATGCCTTGACCAT
ATGTTGTTAAACGAACCAATTTACCTTTGTGCGGTAGAAAGTTACCCTGGTCTTGACCAAGTAATTGAACGATTACAACA
AGCAAATATAAAAAAAGCGCACCTAATGCCGTTTATGCTTGTAGCAGGTGATCACGCTACGAACGATATGGCTTCTGATG
ACGAGGATTCTTGGAAAAGCACGCTGGAACAAGCTGGCATTCAAACTGATTGTCATTTGCAAGGTCTAGGCGAAAATCCG
CTTATTCAAGCCCAATTTATCGATCATATCCACACGGCAATAGAAAGGGTGAAAGCACGTGGCTAA

Upstream 100 bases:

>100_bases
TAGCATCATTCGGTATCAACAATTTTATCGAAGCCGAAAGCGCCACACCAGAGAGCTTAATTGAACTAATCCAAAAAACA
AAATTAGAAGGAGTACCACA

Downstream 100 bases:

>100_bases
ATTTTACGGCATTGGTACAGGTCCTGGAGATAGCAAGTTAGTCACGATGGAAGCAGCAGAAAGACTCGGAAATTTAGAAA
TTCTCTACACGCCACAACCC

Product: anaerobic Cobalt Chelatase In Cobalamin Biosynthesis

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKKAILVVSFGTSYPETREKTIEACEKKVAQEFPDYNVFRAFTSNKIIKKLKTRDNMHINTPSQALNQLKELGYKEVIIQ
SLHIISGGEFEKITAQVEKFKPDFDSIIVSQPLLDSMEDYKKAIEAIRHQMPPLKEQEALILMGHGSKHRAFSAYACLDH
MLLNEPIYLCAVESYPGLDQVIERLQQANIKKAHLMPFMLVAGDHATNDMASDDEDSWKSTLEQAGIQTDCHLQGLGENP
LIQAQFIDHIHTAIERVKARG

Sequences:

>Translated_261_residues
MKKAILVVSFGTSYPETREKTIEACEKKVAQEFPDYNVFRAFTSNKIIKKLKTRDNMHINTPSQALNQLKELGYKEVIIQ
SLHIISGGEFEKITAQVEKFKPDFDSIIVSQPLLDSMEDYKKAIEAIRHQMPPLKEQEALILMGHGSKHRAFSAYACLDH
MLLNEPIYLCAVESYPGLDQVIERLQQANIKKAHLMPFMLVAGDHATNDMASDDEDSWKSTLEQAGIQTDCHLQGLGENP
LIQAQFIDHIHTAIERVKARG
>Mature_261_residues
MKKAILVVSFGTSYPETREKTIEACEKKVAQEFPDYNVFRAFTSNKIIKKLKTRDNMHINTPSQALNQLKELGYKEVIIQ
SLHIISGGEFEKITAQVEKFKPDFDSIIVSQPLLDSMEDYKKAIEAIRHQMPPLKEQEALILMGHGSKHRAFSAYACLDH
MLLNEPIYLCAVESYPGLDQVIERLQQANIKKAHLMPFMLVAGDHATNDMASDDEDSWKSTLEQAGIQTDCHLQGLGENP
LIQAQFIDHIHTAIERVKARG

Specific function: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis [H]

COG id: COG4822

COG function: function code H; Cobalamin biosynthesis protein CbiK, Co2+ chelatase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CbiK family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010388 [H]

Pfam domain/function: PF06180 CbiK [H]

EC number: =4.99.1.3 [H]

Molecular weight: Translated: 29535; Mature: 29535

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAILVVSFGTSYPETREKTIEACEKKVAQEFPDYNVFRAFTSNKIIKKLKTRDNMHIN
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEC
TPSQALNQLKELGYKEVIIQSLHIISGGEFEKITAQVEKFKPDFDSIIVSQPLLDSMEDY
CHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KKAIEAIRHQMPPLKEQEALILMGHGSKHRAFSAYACLDHMLLNEPIYLCAVESYPGLDQ
HHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
VIERLQQANIKKAHLMPFMLVAGDHATNDMASDDEDSWKSTLEQAGIQTDCHLQGLGENP
HHHHHHHCCCCHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCC
LIQAQFIDHIHTAIERVKARG
EEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKAILVVSFGTSYPETREKTIEACEKKVAQEFPDYNVFRAFTSNKIIKKLKTRDNMHIN
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEC
TPSQALNQLKELGYKEVIIQSLHIISGGEFEKITAQVEKFKPDFDSIIVSQPLLDSMEDY
CHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KKAIEAIRHQMPPLKEQEALILMGHGSKHRAFSAYACLDHMLLNEPIYLCAVESYPGLDQ
HHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
VIERLQQANIKKAHLMPFMLVAGDHATNDMASDDEDSWKSTLEQAGIQTDCHLQGLGENP
HHHHHHHCCCCHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCC
LIQAQFIDHIHTAIERVKARG
EEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609; 9150215; 10451360 [H]