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The map label for this gene is cobD

Identifier: 226223792

GI number: 226223792

Start: 1198856

End: 1199803

Strand: Direct

Name: cobD

Synonym: Lm4b_01196

Alternate gene names: 226223792

Gene position: 1198856-1199803 (Clockwise)

Preceding gene: 226223791

Following gene: 226223793

Centisome position: 41.16

GC content: 40.93

Gene sequence:

>948_bases
ATGATATTGTTATTTTATACGTCATCATTCATTTTAGATTGCTTATTAGGTGATCCATATAGCTGGCCGCATCCTATTAA
AGCGATCGGCAACTTGATTAAGTGGTTGACTATTATTTTACGAAAAATCTTTCATGGTAAATCACTGTATTTTGCCGGTG
GATTGCTATTTGTTCTCACGGTTGGTATGACTGGAGTTGTATCCTGGTTCATTCTATTTCTTAGTGCCAAAATTGCTTAC
TGGCTTTACGTAGCTGTTTTTGTTTACTTAGGCTATACGACGCTTGCGATGACTTGCCTTGCGAAAGAGGCTCGGAAAAT
ACAGCGGACCTTGGCGGACGGGGATTTGGCTGCTGCAAGAGTGCAAGTGGGAATGATTGTTGGTCGGGATACGGATAAGT
TAACCGCAGAAGAAATTAGTAAAGCGACGATAGAGACTGTGGCGGAAAATACAGCGGATGGCGTTATCGCACCACTTTTT
TACTTATTCATTGGTGGGCCGGTACTTGCTTTGATGTATAAAGCGGTTAATACACTGGATTCGATGGTCGGTTATAAAAA
CGAAAAATACCGCGCAATTGGTTTTGTTTCCGCGAAAATGGATGACATTGCCAATTTTATCCCAGCGAGATTAGCTTGGT
TTTTCCTTGTCATTGCAAGTTTTATTTTAAGGTATGACGGGCGTGCTTCTTGGCAAATCGGGCTTCGAGATCGGAAAAAC
CATACGAGTCCTAATTGCGCTTATCCTGAAGGTGCAGTAGCAGGAGCGCTTGGAATCACACTTGGTGGAACGCATGAGTA
CTTCGGTGAAACAGTCATTAAACCAACCATTGGAAGTGGAAATAAACCCGTTTCAGAAAAAGAAATTAGCCAAACGATTC
ATTTGCTATATACGGCTTCAACGATCGCTTTTATCATTTTTGCAAGTATTTATCTACTATTATTTTAA

Upstream 100 bases:

>100_bases
ACGGCGGTTATCAAAAAGGTAATACGTTCGCTAGTTACCTGCATATCCATTTCTATCAAAATCTATTGATAATAACGCAT
ATGTTTGGAGCGATAGAGCG

Downstream 100 bases:

>100_bases
AGGAGTGGCAAGATGAACTATATTAAGAATCCAGCCAAAATTGAAGAGAAAAGCTTTGAAATTATTCAACAAATTATTAA
TGATATTCGTCCAGATTACA

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MILLFYTSSFILDCLLGDPYSWPHPIKAIGNLIKWLTIILRKIFHGKSLYFAGGLLFVLTVGMTGVVSWFILFLSAKIAY
WLYVAVFVYLGYTTLAMTCLAKEARKIQRTLADGDLAAARVQVGMIVGRDTDKLTAEEISKATIETVAENTADGVIAPLF
YLFIGGPVLALMYKAVNTLDSMVGYKNEKYRAIGFVSAKMDDIANFIPARLAWFFLVIASFILRYDGRASWQIGLRDRKN
HTSPNCAYPEGAVAGALGITLGGTHEYFGETVIKPTIGSGNKPVSEKEISQTIHLLYTASTIAFIIFASIYLLLF

Sequences:

>Translated_315_residues
MILLFYTSSFILDCLLGDPYSWPHPIKAIGNLIKWLTIILRKIFHGKSLYFAGGLLFVLTVGMTGVVSWFILFLSAKIAY
WLYVAVFVYLGYTTLAMTCLAKEARKIQRTLADGDLAAARVQVGMIVGRDTDKLTAEEISKATIETVAENTADGVIAPLF
YLFIGGPVLALMYKAVNTLDSMVGYKNEKYRAIGFVSAKMDDIANFIPARLAWFFLVIASFILRYDGRASWQIGLRDRKN
HTSPNCAYPEGAVAGALGITLGGTHEYFGETVIKPTIGSGNKPVSEKEISQTIHLLYTASTIAFIIFASIYLLLF
>Mature_315_residues
MILLFYTSSFILDCLLGDPYSWPHPIKAIGNLIKWLTIILRKIFHGKSLYFAGGLLFVLTVGMTGVVSWFILFLSAKIAY
WLYVAVFVYLGYTTLAMTCLAKEARKIQRTLADGDLAAARVQVGMIVGRDTDKLTAEEISKATIETVAENTADGVIAPLF
YLFIGGPVLALMYKAVNTLDSMVGYKNEKYRAIGFVSAKMDDIANFIPARLAWFFLVIASFILRYDGRASWQIGLRDRKN
HTSPNCAYPEGAVAGALGITLGGTHEYFGETVIKPTIGSGNKPVSEKEISQTIHLLYTASTIAFIIFASIYLLLF

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBD_LISMF (Q720N7)

Other databases:

- EMBL:   AE017262
- RefSeq:   YP_013800.1
- STRING:   Q720N7
- GeneID:   2798906
- GenomeReviews:   AE017262_GR
- KEGG:   lmf:LMOf2365_1201
- TIGR:   LMOf2365_1201
- eggNOG:   COG1270
- HOGENOM:   HBG647131
- OMA:   IGDPWGW
- ProtClustDB:   PRK01209
- BioCyc:   LMON265669:LMOF2365_1201-MONOMER
- HAMAP:   MF_00024
- InterPro:   IPR004485
- TIGRFAMs:   TIGR00380

Pfam domain/function: PF03186 CobD_Cbib

EC number: 6.3.1.10

Molecular weight: Translated: 34762; Mature: 34762

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1ed9f5cc)-; HASH(0x1f248f84)-; HASH(0x1f24a754)-; HASH(0x1e941640)-; HASH(0x1f247604)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLFYTSSFILDCLLGDPYSWPHPIKAIGNLIKWLTIILRKIFHGKSLYFAGGLLFVLT
CEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VGMTGVVSWFILFLSAKIAYWLYVAVFVYLGYTTLAMTCLAKEARKIQRTLADGDLAAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VQVGMIVGRDTDKLTAEEISKATIETVAENTADGVIAPLFYLFIGGPVLALMYKAVNTLD
HHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMVGYKNEKYRAIGFVSAKMDDIANFIPARLAWFFLVIASFILRYDGRASWQIGLRDRKN
HHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
HTSPNCAYPEGAVAGALGITLGGTHEYFGETVIKPTIGSGNKPVSEKEISQTIHLLYTAS
CCCCCCCCCCCHHHHHHHEEECCCHHHHCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TIAFIIFASIYLLLF
HHHHHHHHHHHHHHH
>Mature Secondary Structure
MILLFYTSSFILDCLLGDPYSWPHPIKAIGNLIKWLTIILRKIFHGKSLYFAGGLLFVLT
CEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VGMTGVVSWFILFLSAKIAYWLYVAVFVYLGYTTLAMTCLAKEARKIQRTLADGDLAAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VQVGMIVGRDTDKLTAEEISKATIETVAENTADGVIAPLFYLFIGGPVLALMYKAVNTLD
HHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMVGYKNEKYRAIGFVSAKMDDIANFIPARLAWFFLVIASFILRYDGRASWQIGLRDRKN
HHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
HTSPNCAYPEGAVAGALGITLGGTHEYFGETVIKPTIGSGNKPVSEKEISQTIHLLYTAS
CCCCCCCCCCCHHHHHHHEEECCCHHHHCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TIAFIIFASIYLLLF
HHHHHHHHHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA