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The map label for this gene is pduC [H]

Identifier: 226223754

GI number: 226223754

Start: 1164779

End: 1166443

Strand: Direct

Name: pduC [H]

Synonym: Lm4b_01158

Alternate gene names: 226223754

Gene position: 1164779-1166443 (Clockwise)

Preceding gene: 226223753

Following gene: 226223755

Centisome position: 39.99

GC content: 41.26

Gene sequence:

>1665_bases
ATGAAATCAAAACGCTTTGAAGAATTAGCAAAACGCCCGGTCAATCAAGATGGTTTTGTAAAAGAATGGATTGAAGAAGG
CCTAATCGCAATGGAAAGCCCGAATGATCCGAAACCAAGCATTAAAATAGAAAATGGAAAAGTAGTCGAAATGGACAGCA
AAAAACTCGCTGACTTTGACTTAATTGACCATTTCATCGCTAAATATGGCGTCGATTTATCTCGTGCGGAAGAAGTTATG
CAAATGGATTCTGTGAAGCTCGCAAATATGCTTTGCGACCCAAATGTGCCACGGGAAAAAATCGTTTTACTTACAACAGC
TATGACACCGGCAAAAATAGTAGAAGTAGTTTCACAAATGAACGTTGTCGAAATGATGATGTCGATGCAAAAAATGCGCT
CTCGCAGAACACCAACAACTCAGGCCCACGTAACAAACTTGCGTGATAATCCTGTTCAAATTGCTGCAGATGCGGCAGAA
GCAGCAATTCGTGGTTTTGACGAGCAAGAAACAACCGTTGCGGTAGTTCGTTATGCACCTTTTAACGCGCTTAGTTTATT
AGTAGGTTCGCAAACTGGCCGTGGTGGCGTATTAACGCAATGTTCTCTCGAAGAAGCAACAGAATTAGAACTCGGTATGC
GTGGTTTAACTTGTTATGCGGAAACGATTTCTGTTTATGGTACAGAACCTGTATTTACAGACGGAGACGATACACCTTGG
TCCAAAGGTATTTTGGCAAGTGCTTATGCATCTCGAGGTCTGAAAATGCGCTTCACATCCGGAACTGGTTCTGAAGTTCA
AATGGGTTACGCAGAAGGTAAATCGATGCTTTATCTTGAATCTCGCTGTATTTTCATTACGAAAGCGGCTGGTGTTCAAG
GGTTACAAAATGGTTCGATTAGTTGTATCGGAATTCCGGGAGCCGTACCAAGTGGTATTAGAGCGGTTCTTGCAGAGAAT
TTAATTGCCGTAATGTTAGATCTAGAAGTAGCTTCTGGTAACGACCAAACATTCTCGCACTCGGATATTCGTCGTACAGC
TCGTTTATTGATGCAATTTTTACCAGGAACAGATTATATTTCCTCTGGTTACAGTGCAACACCTAACTATGACAATATGT
TTGCTGGTTCCAATTTTGATGCAGATGACTTTGATGATTACAATATTTTGCAACGTGATTTAAAAGTAGACGGTGGTTTA
ACGCCAGTTACCGAAGAAGAAGTCGTTGCAGTTAGAAATAAAGCGGCACGTGTAATTCAAGCTGTCTTTGATAAATTAGG
TCTTCCAGAAGTAACCGATGCGGAAGTAGAAGCAGCAACGTATGCACGTGGAAGTAAAGATATGCCAGAACGTAACATGG
TAGAAGATATTAAAGCAGCTGCTGAAATGATGGACCGTGGTGTTACTGGTCTGGATGTTGTTAAAGCGTTATCTGCTGGC
GGATTCGATGATGTTGCTGAAAGTGTACTAAATATGTTGAAACAACGTGTATCTGGGGACTTCCTTCATACATCTGCCAT
CATTGATAAACACTGGAATGTCATTAGTTCCGTCAATGACTTAAATGATTATGCAGGTCCTGGAACTGGTTATCGCTTAG
AGGGCGAACGCTGGGAGAAATTAAAAGATATTGCTGTTGCAGTTGATGCAAACGAATTAGAATAA

Upstream 100 bases:

>100_bases
TATTTCAGCACGTGAAATTGGTAAAAAATTACTTGGAGCTTTAGGGGATGAACCGAAAAATGATCGTCCATCCTACATTT
AGAACGGAGGGTAACGACTG

Downstream 100 bases:

>100_bases
GCTACTTCCCATTTTTTATAAAGGAGGATTAAGAGACATGGTTGAAATTAACGAAAAAGTACTCCGCGGAATTATCTCGG
AAGTACTAGATGAATTACAG

Product: propanediol dehydratase, alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKSKRFEELAKRPVNQDGFVKEWIEEGLIAMESPNDPKPSIKIENGKVVEMDSKKLADFDLIDHFIAKYGVDLSRAEEVM
QMDSVKLANMLCDPNVPREKIVLLTTAMTPAKIVEVVSQMNVVEMMMSMQKMRSRRTPTTQAHVTNLRDNPVQIAADAAE
AAIRGFDEQETTVAVVRYAPFNALSLLVGSQTGRGGVLTQCSLEEATELELGMRGLTCYAETISVYGTEPVFTDGDDTPW
SKGILASAYASRGLKMRFTSGTGSEVQMGYAEGKSMLYLESRCIFITKAAGVQGLQNGSISCIGIPGAVPSGIRAVLAEN
LIAVMLDLEVASGNDQTFSHSDIRRTARLLMQFLPGTDYISSGYSATPNYDNMFAGSNFDADDFDDYNILQRDLKVDGGL
TPVTEEEVVAVRNKAARVIQAVFDKLGLPEVTDAEVEAATYARGSKDMPERNMVEDIKAAAEMMDRGVTGLDVVKALSAG
GFDDVAESVLNMLKQRVSGDFLHTSAIIDKHWNVISSVNDLNDYAGPGTGYRLEGERWEKLKDIAVAVDANELE

Sequences:

>Translated_554_residues
MKSKRFEELAKRPVNQDGFVKEWIEEGLIAMESPNDPKPSIKIENGKVVEMDSKKLADFDLIDHFIAKYGVDLSRAEEVM
QMDSVKLANMLCDPNVPREKIVLLTTAMTPAKIVEVVSQMNVVEMMMSMQKMRSRRTPTTQAHVTNLRDNPVQIAADAAE
AAIRGFDEQETTVAVVRYAPFNALSLLVGSQTGRGGVLTQCSLEEATELELGMRGLTCYAETISVYGTEPVFTDGDDTPW
SKGILASAYASRGLKMRFTSGTGSEVQMGYAEGKSMLYLESRCIFITKAAGVQGLQNGSISCIGIPGAVPSGIRAVLAEN
LIAVMLDLEVASGNDQTFSHSDIRRTARLLMQFLPGTDYISSGYSATPNYDNMFAGSNFDADDFDDYNILQRDLKVDGGL
TPVTEEEVVAVRNKAARVIQAVFDKLGLPEVTDAEVEAATYARGSKDMPERNMVEDIKAAAEMMDRGVTGLDVVKALSAG
GFDDVAESVLNMLKQRVSGDFLHTSAIIDKHWNVISSVNDLNDYAGPGTGYRLEGERWEKLKDIAVAVDANELE
>Mature_554_residues
MKSKRFEELAKRPVNQDGFVKEWIEEGLIAMESPNDPKPSIKIENGKVVEMDSKKLADFDLIDHFIAKYGVDLSRAEEVM
QMDSVKLANMLCDPNVPREKIVLLTTAMTPAKIVEVVSQMNVVEMMMSMQKMRSRRTPTTQAHVTNLRDNPVQIAADAAE
AAIRGFDEQETTVAVVRYAPFNALSLLVGSQTGRGGVLTQCSLEEATELELGMRGLTCYAETISVYGTEPVFTDGDDTPW
SKGILASAYASRGLKMRFTSGTGSEVQMGYAEGKSMLYLESRCIFITKAAGVQGLQNGSISCIGIPGAVPSGIRAVLAEN
LIAVMLDLEVASGNDQTFSHSDIRRTARLLMQFLPGTDYISSGYSATPNYDNMFAGSNFDADDFDDYNILQRDLKVDGGL
TPVTEEEVVAVRNKAARVIQAVFDKLGLPEVTDAEVEAATYARGSKDMPERNMVEDIKAAAEMMDRGVTGLDVVKALSAG
GFDDVAESVLNMLKQRVSGDFLHTSAIIDKHWNVISSVNDLNDYAGPGTGYRLEGERWEKLKDIAVAVDANELE

Specific function: Part of the pduCDE complex that catalyzes the dehydration of 1,2-propanediol to propionaldehyde. Is required for S.typhimurium growth on 1,2-propanediol as the sole carbon and energy source [H]

COG id: COG4909

COG function: function code Q; Propanediol dehydratase, large subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diol/glycerol dehydratase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016176
- InterPro:   IPR003206
- ProDom:   PD025428 [H]

Pfam domain/function: PF02286 Dehydratase_LU [H]

EC number: =4.2.1.28 [H]

Molecular weight: Translated: 60475; Mature: 60475

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKRFEELAKRPVNQDGFVKEWIEEGLIAMESPNDPKPSIKIENGKVVEMDSKKLADFD
CCCHHHHHHHHCCCCCCCHHHHHHHCCCEEECCCCCCCCCEEECCCEEEEECCCCCCHHH
LIDHFIAKYGVDLSRAEEVMQMDSVKLANMLCDPNVPREKIVLLTTAMTPAKIVEVVSQM
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHH
NVVEMMMSMQKMRSRRTPTTQAHVTNLRDNPVQIAADAAEAAIRGFDEQETTVAVVRYAP
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEHHHHHHHHHCCCCCCCEEEEEEECC
FNALSLLVGSQTGRGGVLTQCSLEEATELELGMRGLTCYAETISVYGTEPVFTDGDDTPW
CCHHHHHHCCCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCCCCC
SKGILASAYASRGLKMRFTSGTGSEVQMGYAEGKSMLYLESRCIFITKAAGVQGLQNGSI
CCCHHHHHHHHCCEEEEEECCCCCCEEEEHHCCCEEEEEECCEEEEEECCCCCCCCCCCE
SCIGIPGAVPSGIRAVLAENLIAVMLDLEVASGNDQTFSHSDIRRTARLLMQFLPGTDYI
EEEECCCCCCHHHHHHHHHHHHEEHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHH
SSGYSATPNYDNMFAGSNFDADDFDDYNILQRDLKVDGGLTPVTEEEVVAVRNKAARVIQ
HCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
AVFDKLGLPEVTDAEVEAATYARGSKDMPERNMVEDIKAAAEMMDRGVTGLDVVKALSAG
HHHHHCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
GFDDVAESVLNMLKQRVSGDFLHTSAIIDKHWNVISSVNDLNDYAGPGTGYRLEGERWEK
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHH
LKDIAVAVDANELE
HHHHEEEECCCCCC
>Mature Secondary Structure
MKSKRFEELAKRPVNQDGFVKEWIEEGLIAMESPNDPKPSIKIENGKVVEMDSKKLADFD
CCCHHHHHHHHCCCCCCCHHHHHHHCCCEEECCCCCCCCCEEECCCEEEEECCCCCCHHH
LIDHFIAKYGVDLSRAEEVMQMDSVKLANMLCDPNVPREKIVLLTTAMTPAKIVEVVSQM
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHH
NVVEMMMSMQKMRSRRTPTTQAHVTNLRDNPVQIAADAAEAAIRGFDEQETTVAVVRYAP
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEHHHHHHHHHCCCCCCCEEEEEEECC
FNALSLLVGSQTGRGGVLTQCSLEEATELELGMRGLTCYAETISVYGTEPVFTDGDDTPW
CCHHHHHHCCCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCCCCC
SKGILASAYASRGLKMRFTSGTGSEVQMGYAEGKSMLYLESRCIFITKAAGVQGLQNGSI
CCCHHHHHHHHCCEEEEEECCCCCCEEEEHHCCCEEEEEECCEEEEEECCCCCCCCCCCE
SCIGIPGAVPSGIRAVLAENLIAVMLDLEVASGNDQTFSHSDIRRTARLLMQFLPGTDYI
EEEECCCCCCHHHHHHHHHHHHEEHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHH
SSGYSATPNYDNMFAGSNFDADDFDDYNILQRDLKVDGGLTPVTEEEVVAVRNKAARVIQ
HCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
AVFDKLGLPEVTDAEVEAATYARGSKDMPERNMVEDIKAAAEMMDRGVTGLDVVKALSAG
HHHHHCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
GFDDVAESVLNMLKQRVSGDFLHTSAIIDKHWNVISSVNDLNDYAGPGTGYRLEGERWEK
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHH
LKDIAVAVDANELE
HHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9352910; 11677609; 8071226 [H]