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The map label for this gene is metN [H]

Identifier: 226223709

GI number: 226223709

Start: 1127334

End: 1128227

Strand: Direct

Name: metN [H]

Synonym: Lm4b_01111

Alternate gene names: 226223709

Gene position: 1127334-1128227 (Clockwise)

Preceding gene: 226223708

Following gene: 226223710

Centisome position: 38.7

GC content: 30.2

Gene sequence:

>894_bases
GTGCATATGAATAATATTTTGGAAACGAAAAATTTGAAAGTTACAATAAATAATAAAGTAATTCTATATTTAGATAAAGA
AGTTTGTATTTCAGAAAAAGACAAAGTTGCCATTCTAGGAGACAATGGAGCTGGGAAAACCACGTTAGTAAATAGTATTC
TTGGTGAAAAGAACTCTTCTGGAGAAATTACCAAAAAGTTTAAAAAAAATGACTGTGGTGTAGTGTTTCAAGAAAATGCA
TATAATGATTTGATGAAAGTTTATGAATTGATTACTTTAGTTCTGCCACATCTAAAGAAAAAAGAGAGAGCGCAGTTTTT
ACACAAATATGAACTTGAAAGTTTGAGAAAAAAGTACATTAAAGACTTATCTGGTGGAGAAAAACAACGACTAACACTAT
GTTTAGTACTAGAAAGTCATAAGAAATTATATATCTTTGATGAGTTAACTTCAGGATTAGATTATAAGAAGAGACTAGGT
TTACTTGCTTTGATGAAAGAAAAAACTAAGGACGCGACGGTGATAAATATAACGCATTACTTTGAGGAAGTTGAAAACTG
GGCAACGAAAGTTTTGATTCTTCAAAAAGGAATTTTACTTTTTTGGGGAACAATATCAGAATTTTTCTCTAATTTTCCTC
ATTATTCTGTTATTAAAGTAGATCAAATCGAACTAACAAAGATAGATGAAACAGATATGACTTTTATGCAAAGCACAGAT
ACAGGTGACGGGATAGCAGTTATTTGTTCGGATTTGCAAATTCAGGAAGAGACAAAGAAAATTTTGGATAAAAAAAACGT
CACATACAACACAATAAAACAAAATATATATACAACTTATTTAGTTGCATATTTGCGCGGAACATCAAGCAGTGAACAGG
AGGTACTGATATAA

Upstream 100 bases:

>100_bases
TGTACATGTACATGCGCATCATCTGCTGCAACCAAAATGTAAATTTTAGAATGAAAAAGGGATACATTTGTGTCCCTTTT
CAATAAGTTATGAGGTGCAT

Downstream 100 bases:

>100_bases
TGAGTTATTTATGGACAAGTATTAAAATGCAATTTAGAATTCCAGTATCTGTGTTTTTCTCATTATTATTTCCACTAATC
ATGATGTTCGCTATGGTTAC

Product: ABC transporter (ATP binding protein)

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MHMNNILETKNLKVTINNKVILYLDKEVCISEKDKVAILGDNGAGKTTLVNSILGEKNSSGEITKKFKKNDCGVVFQENA
YNDLMKVYELITLVLPHLKKKERAQFLHKYELESLRKKYIKDLSGGEKQRLTLCLVLESHKKLYIFDELTSGLDYKKRLG
LLALMKEKTKDATVINITHYFEEVENWATKVLILQKGILLFWGTISEFFSNFPHYSVIKVDQIELTKIDETDMTFMQSTD
TGDGIAVICSDLQIQEETKKILDKKNVTYNTIKQNIYTTYLVAYLRGTSSSEQEVLI

Sequences:

>Translated_297_residues
MHMNNILETKNLKVTINNKVILYLDKEVCISEKDKVAILGDNGAGKTTLVNSILGEKNSSGEITKKFKKNDCGVVFQENA
YNDLMKVYELITLVLPHLKKKERAQFLHKYELESLRKKYIKDLSGGEKQRLTLCLVLESHKKLYIFDELTSGLDYKKRLG
LLALMKEKTKDATVINITHYFEEVENWATKVLILQKGILLFWGTISEFFSNFPHYSVIKVDQIELTKIDETDMTFMQSTD
TGDGIAVICSDLQIQEETKKILDKKNVTYNTIKQNIYTTYLVAYLRGTSSSEQEVLI
>Mature_297_residues
MHMNNILETKNLKVTINNKVILYLDKEVCISEKDKVAILGDNGAGKTTLVNSILGEKNSSGEITKKFKKNDCGVVFQENA
YNDLMKVYELITLVLPHLKKKERAQFLHKYELESLRKKYIKDLSGGEKQRLTLCLVLESHKKLYIFDELTSGLDYKKRLG
LLALMKEKTKDATVINITHYFEEVENWATKVLILQKGILLFWGTISEFFSNFPHYSVIKVDQIELTKIDETDMTFMQSTD
TGDGIAVICSDLQIQEETKKILDKKNVTYNTIKQNIYTTYLVAYLRGTSSSEQEVLI

Specific function: Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system [H]

COG id: COG1131

COG function: function code V; ABC-type multidrug transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=186, Percent_Identity=30.6451612903226, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI27477115, Length=192, Percent_Identity=28.125, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI153792144, Length=187, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI27881501, Length=240, Percent_Identity=27.5, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI30795238, Length=240, Percent_Identity=27.5, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788761, Length=214, Percent_Identity=29.4392523364486, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081709, Length=227, Percent_Identity=28.6343612334802, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786253, Length=207, Percent_Identity=27.0531400966184, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786319, Length=215, Percent_Identity=30.6976744186047, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787029, Length=234, Percent_Identity=26.4957264957265, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1786563, Length=189, Percent_Identity=32.2751322751323, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789891, Length=190, Percent_Identity=30, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788506, Length=297, Percent_Identity=22.8956228956229, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786398, Length=199, Percent_Identity=28.643216080402, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI48994883, Length=251, Percent_Identity=24.3027888446215, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787370, Length=209, Percent_Identity=27.7511961722488, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71983523, Length=183, Percent_Identity=29.5081967213115, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71983516, Length=183, Percent_Identity=29.5081967213115, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71983510, Length=183, Percent_Identity=29.5081967213115, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=180, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI116007328, Length=209, Percent_Identity=31.1004784688995, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI28574150, Length=209, Percent_Identity=31.1004784688995, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24662800, Length=279, Percent_Identity=23.2974910394265, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221512771, Length=185, Percent_Identity=30.8108108108108, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24643674, Length=219, Percent_Identity=27.8538812785388, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581407, Length=242, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR012692
- InterPro:   IPR017908
- InterPro:   IPR003593
- InterPro:   IPR018449 [H]

Pfam domain/function: PF00005 ABC_tran; PF09383 NIL [H]

EC number: 3.6.3.25 [C]

Molecular weight: Translated: 34100; Mature: 34100

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMNNILETKNLKVTINNKVILYLDKEVCISEKDKVAILGDNGAGKTTLVNSILGEKNSS
CCCCHHCCCCCEEEEECCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCC
GEITKKFKKNDCGVVFQENAYNDLMKVYELITLVLPHLKKKERAQFLHKYELESLRKKYI
CHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KDLSGGEKQRLTLCLVLESHKKLYIFDELTSGLDYKKRLGLLALMKEKTKDATVINITHY
HHCCCCCCCEEEEEEEEECCCEEEEEEHHHCCCCHHHHHHHHHHHHHCCCCCEEEEHHHH
FEEVENWATKVLILQKGILLFWGTISEFFSNFPHYSVIKVDQIELTKIDETDMTFMQSTD
HHHHHHHHHHHHHHHCCHHEEHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCEEECCC
TGDGIAVICSDLQIQEETKKILDKKNVTYNTIKQNIYTTYLVAYLRGTSSSEQEVLI
CCCCEEEEEECCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MHMNNILETKNLKVTINNKVILYLDKEVCISEKDKVAILGDNGAGKTTLVNSILGEKNSS
CCCCHHCCCCCEEEEECCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCC
GEITKKFKKNDCGVVFQENAYNDLMKVYELITLVLPHLKKKERAQFLHKYELESLRKKYI
CHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KDLSGGEKQRLTLCLVLESHKKLYIFDELTSGLDYKKRLGLLALMKEKTKDATVINITHY
HHCCCCCCCEEEEEEEEECCCEEEEEEHHHCCCCHHHHHHHHHHHHHCCCCCEEEEHHHH
FEEVENWATKVLILQKGILLFWGTISEFFSNFPHYSVIKVDQIELTKIDETDMTFMQSTD
HHHHHHHHHHHHHHHCCHHEEHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCEEECCC
TGDGIAVICSDLQIQEETKKILDKKNVTYNTIKQNIYTTYLVAYLRGTSSSEQEVLI
CCCCEEEEEECCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA