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The map label for this gene is tagF [H]

Identifier: 226223700

GI number: 226223700

Start: 1118255

End: 1119409

Strand: Direct

Name: tagF [H]

Synonym: Lm4b_01102

Alternate gene names: 226223700

Gene position: 1118255-1119409 (Clockwise)

Preceding gene: 226223699

Following gene: 226223701

Centisome position: 38.39

GC content: 34.37

Gene sequence:

>1155_bases
ATGAAGTTAGTACAAAAAGTGTATTATTTGTTGTTTAGATTAGTGGGATTTTTACCGCGAAAAAAAGATTTAGTGATATT
TGAAAGCTTTTCAGGTAAGCAATATAGCTGTAATCCACGGGCTATTTATGAATATATGGAGGAGCATAATCCGGAATACG
AATTATTATGGAGTGTTAATCCAAAATTCGTGGCTGTTTTTGAAGCTTACGGAGTTCCTTATGTTAAACGTTTTTCTATT
AGCTGGTTATTTAAAATGGGGCTTGCTAAGTATTGGATTTCTAATAGTAGACTTCCTTTAGAACTACCAAAACCGAAAAA
AACAATCTATGTGCAAACTTGGCATGGAACACCCTTAAAAAAATTAGGCGTGGATATTGATGAAGTGCACATTCCAGGGC
AAACAACGGAACAGTACAAAGCTGATTTTGTAAAAGAAGCACAGAAATGGGACTACTTAATTTCTCCTAATGCTTATTCT
AGTGCTATTTTTAGACGGGCATTTGGTTTTACTGGAGAGATGATTGAGTCGGGATATCCGCGGAATGATATTTTATTTAG
TACCGATAAAGAGCTGAAAATAGCTAATATTAAAAAAGAGCTAAATATCCCAGAAGAGAAAAAAGTTGTTTTATATGCGC
CAACATGGCGTGATAATGATTTTTATGAAGCTGGAAGATATAAATTTGATTTAAAAATCGATATCGCAAAAATGCAAGAA
AAATTGGGGAATGAGATTGTTTTACTTGTTCGCATGCATTATTTAGTCGCGGAACATTTTGATTTCACGCAATATGGTGA
TTTTGTCCGCGATGCTTCAAACCATGAAGATATACGCGATTTATACTTAGTGAGTGATTTGTTGATTACTGATTATTCCT
CTGTATTTTTTGATTATGCGAATTTACAGCGTCCTATGCTTTTTTATACGTATGATTTGGCGGAATATCGTGATACGTTA
CGTGGCTTTTACTTTGATTTTGAAAAAAATGCTCCTGGTCCGCTTGTGGAAACGAATGAAGAATTAATGAGTGAACTTGA
AAAAATGCTTGAAAATCCACCTAAAATAGAAGACAGCTTTTTGGAGCAGTTTTGTACTTGGGAAGATGGTCATGCAGCAG
AGAAAACAGTGAAAATCGTTTTTGCTGAAAAATAG

Upstream 100 bases:

>100_bases
ATACGCCTATCGCCGACATAAATATCATAAGTAGCATCTTAGGGAAATTAAAACGAAATCGAGCGTTAAAGCGAAAGTTT
AAACAGGGGGGAGTATCCTG

Downstream 100 bases:

>100_bases
GTGGTGGGAATTTTGAAAGAAGTAGCAATTTATATTTATATGCTTGCTGTTAAGATAACTGGCTGTTTGGCGAGGATTTT
TCCAGTTAAACAAAAAGTGG

Product: TagF protein (probable CDPglycerol glycerophosphotransferase)

Products: NA

Alternate protein names: CGPTase; Major teichoic acid biosynthesis protein F; Polyglycerol phosphate polymerase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKLVQKVYYLLFRLVGFLPRKKDLVIFESFSGKQYSCNPRAIYEYMEEHNPEYELLWSVNPKFVAVFEAYGVPYVKRFSI
SWLFKMGLAKYWISNSRLPLELPKPKKTIYVQTWHGTPLKKLGVDIDEVHIPGQTTEQYKADFVKEAQKWDYLISPNAYS
SAIFRRAFGFTGEMIESGYPRNDILFSTDKELKIANIKKELNIPEEKKVVLYAPTWRDNDFYEAGRYKFDLKIDIAKMQE
KLGNEIVLLVRMHYLVAEHFDFTQYGDFVRDASNHEDIRDLYLVSDLLITDYSSVFFDYANLQRPMLFYTYDLAEYRDTL
RGFYFDFEKNAPGPLVETNEELMSELEKMLENPPKIEDSFLEQFCTWEDGHAAEKTVKIVFAEK

Sequences:

>Translated_384_residues
MKLVQKVYYLLFRLVGFLPRKKDLVIFESFSGKQYSCNPRAIYEYMEEHNPEYELLWSVNPKFVAVFEAYGVPYVKRFSI
SWLFKMGLAKYWISNSRLPLELPKPKKTIYVQTWHGTPLKKLGVDIDEVHIPGQTTEQYKADFVKEAQKWDYLISPNAYS
SAIFRRAFGFTGEMIESGYPRNDILFSTDKELKIANIKKELNIPEEKKVVLYAPTWRDNDFYEAGRYKFDLKIDIAKMQE
KLGNEIVLLVRMHYLVAEHFDFTQYGDFVRDASNHEDIRDLYLVSDLLITDYSSVFFDYANLQRPMLFYTYDLAEYRDTL
RGFYFDFEKNAPGPLVETNEELMSELEKMLENPPKIEDSFLEQFCTWEDGHAAEKTVKIVFAEK
>Mature_384_residues
MKLVQKVYYLLFRLVGFLPRKKDLVIFESFSGKQYSCNPRAIYEYMEEHNPEYELLWSVNPKFVAVFEAYGVPYVKRFSI
SWLFKMGLAKYWISNSRLPLELPKPKKTIYVQTWHGTPLKKLGVDIDEVHIPGQTTEQYKADFVKEAQKWDYLISPNAYS
SAIFRRAFGFTGEMIESGYPRNDILFSTDKELKIANIKKELNIPEEKKVVLYAPTWRDNDFYEAGRYKFDLKIDIAKMQE
KLGNEIVLLVRMHYLVAEHFDFTQYGDFVRDASNHEDIRDLYLVSDLLITDYSSVFFDYANLQRPMLFYTYDLAEYRDTL
RGFYFDFEKNAPGPLVETNEELMSELEKMLENPPKIEDSFLEQFCTWEDGHAAEKTVKIVFAEK

Specific function: Is responsible for the polymerization of the main chain of the major teichoic acid by sequential transfer of glycerol phosphate units from CDP-glycerol to the dissacharide linkage unit. Synthesizes polymers of approximately 35 glycerol phosphate units in

COG id: COG1887

COG function: function code M; Putative glycosyl/glycerophosphate transferases involved in teichoic acid biosynthesis TagF/TagB/EpsJ/RodC

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CDP-glycerol glycerophosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007554 [H]

Pfam domain/function: PF04464 Glyphos_transf [H]

EC number: =2.7.8.12 [H]

Molecular weight: Translated: 45365; Mature: 45365

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVQKVYYLLFRLVGFLPRKKDLVIFESFSGKQYSCNPRAIYEYMEEHNPEYELLWSVN
CHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCHHHHHHHHHHCCCCEEEEEECC
PKFVAVFEAYGVPYVKRFSISWLFKMGLAKYWISNSRLPLELPKPKKTIYVQTWHGTPLK
CCEEEEEEHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEEEECCCCHH
KLGVDIDEVHIPGQTTEQYKADFVKEAQKWDYLISPNAYSSAIFRRAFGFTGEMIESGYP
HHCCCHHEEECCCCCHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCCHHHHHCCCC
RNDILFSTDKELKIANIKKELNIPEEKKVVLYAPTWRDNDFYEAGRYKFDLKIDIAKMQE
CCCEEEECCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEHHHHHH
KLGNEIVLLVRMHYLVAEHFDFTQYGDFVRDASNHEDIRDLYLVSDLLITDYSSVFFDYA
HHCCCEEHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NLQRPMLFYTYDLAEYRDTLRGFYFDFEKNAPGPLVETNEELMSELEKMLENPPKIEDSF
HCCCCEEEEEECHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHH
LEQFCTWEDGHAAEKTVKIVFAEK
HHHHHCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKLVQKVYYLLFRLVGFLPRKKDLVIFESFSGKQYSCNPRAIYEYMEEHNPEYELLWSVN
CHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCHHHHHHHHHHCCCCEEEEEECC
PKFVAVFEAYGVPYVKRFSISWLFKMGLAKYWISNSRLPLELPKPKKTIYVQTWHGTPLK
CCEEEEEEHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEEEECCCCHH
KLGVDIDEVHIPGQTTEQYKADFVKEAQKWDYLISPNAYSSAIFRRAFGFTGEMIESGYP
HHCCCHHEEECCCCCHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCCHHHHHCCCC
RNDILFSTDKELKIANIKKELNIPEEKKVVLYAPTWRDNDFYEAGRYKFDLKIDIAKMQE
CCCEEEECCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEHHHHHH
KLGNEIVLLVRMHYLVAEHFDFTQYGDFVRDASNHEDIRDLYLVSDLLITDYSSVFFDYA
HHCCCEEHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NLQRPMLFYTYDLAEYRDTLRGFYFDFEKNAPGPLVETNEELMSELEKMLENPPKIEDSF
HCCCCEEEEEECHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHH
LEQFCTWEDGHAAEKTVKIVFAEK
HHHHHCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2507871; 9384377; 1309530; 12637499 [H]