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The map label for this gene is ykwC [H]

Identifier: 226223623

GI number: 226223623

Start: 1037125

End: 1037985

Strand: Direct

Name: ykwC [H]

Synonym: Lm4b_01025

Alternate gene names: 226223623

Gene position: 1037125-1037985 (Clockwise)

Preceding gene: 226223622

Following gene: 226223625

Centisome position: 35.61

GC content: 40.65

Gene sequence:

>861_bases
ATGGAGAAAATTGGATTTGTTGGTACAGGCGTAATGGGTTCGAGTATGGCAGGGCATTTACTGGAGGCTGGTTATGAAGT
ATTGGTGTATACTCGCACAAAAACAAAAGCAGAAGATTTACTTGAAAAAGGAGCACTATGGATAAAAACACCAGGAGAAT
TGGCTAATAAAGTGGATATATTAATTTCAATGGTTGGTTATCCAAAAGATGTGGAAGAGCTTTACTTAGGCGAAAATGGC
TTTTTAGAAAATTTAGCGGTTGGCACAGTAGCAATCGATATGACAACTTCCTCACCGGCATTAGCTAAAAAAATGGCTGA
ATTTGGCCGTGAAAAAGGAATCGGTGTACTCGATGCTCCTGTTTCAGGTGGCGACATAGGCGCGAAAAATGGCACGCTCG
CAATTATGGTAGGAGGATCTGAAGACGTATTTTTAAAAGTGAAACCAATTTTTGACATTCTTGGTAGCAGTGTGATTCTG
CAAGGTGATGCAGGTGCTGGACAACATACCAAAATGGTGAATCAAATTGCAATTGCTTCCAACATGATTGGTGTGACTGA
AGCGATCATTTATGCGGAGGCGGCTGGACTTAATCCATCAAGTGTTCTCGATTCAATTTCAGGTGGTGCAGCAGGCAGTT
GGTCGCTTGCAAACTTGATTCCACGTGTACTTAAGGATGATTTTTCTCCGGGTTTCTTTATTAAGCATTTTATAAAAGAT
ATGGGAATCGCAATCTCAGAAGCAAAACAAATGGGCTTAGAACTTCCTGGGCTAACTTTAGCTGAAAAAATGTATCAAAC
GCTAGCTGAACAAGGTTTGAGTGAAGAAGGGACGCAAGCGCTCATTAAATATTATCGTTAA

Upstream 100 bases:

>100_bases
AATGTATAAAAAAGTACATATGGTCTTACTTGGTTTATTACTATTGTTTTTTGCGTTTTTAGCGATTATAGGTGTTACTT
TTAGATAGGAGGTTAGCCTG

Downstream 100 bases:

>100_bases
AAAAACATCCACTAATTTAAGTTAGTGGATGTTTTTTTATTTTGCTATAAACTGAGCCATTCGATCTAATGCTTCAGCAA
GATTATTAAAGGAAGTAGCA

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MEKIGFVGTGVMGSSMAGHLLEAGYEVLVYTRTKTKAEDLLEKGALWIKTPGELANKVDILISMVGYPKDVEELYLGENG
FLENLAVGTVAIDMTTSSPALAKKMAEFGREKGIGVLDAPVSGGDIGAKNGTLAIMVGGSEDVFLKVKPIFDILGSSVIL
QGDAGAGQHTKMVNQIAIASNMIGVTEAIIYAEAAGLNPSSVLDSISGGAAGSWSLANLIPRVLKDDFSPGFFIKHFIKD
MGIAISEAKQMGLELPGLTLAEKMYQTLAEQGLSEEGTQALIKYYR

Sequences:

>Translated_286_residues
MEKIGFVGTGVMGSSMAGHLLEAGYEVLVYTRTKTKAEDLLEKGALWIKTPGELANKVDILISMVGYPKDVEELYLGENG
FLENLAVGTVAIDMTTSSPALAKKMAEFGREKGIGVLDAPVSGGDIGAKNGTLAIMVGGSEDVFLKVKPIFDILGSSVIL
QGDAGAGQHTKMVNQIAIASNMIGVTEAIIYAEAAGLNPSSVLDSISGGAAGSWSLANLIPRVLKDDFSPGFFIKHFIKD
MGIAISEAKQMGLELPGLTLAEKMYQTLAEQGLSEEGTQALIKYYR
>Mature_286_residues
MEKIGFVGTGVMGSSMAGHLLEAGYEVLVYTRTKTKAEDLLEKGALWIKTPGELANKVDILISMVGYPKDVEELYLGENG
FLENLAVGTVAIDMTTSSPALAKKMAEFGREKGIGVLDAPVSGGDIGAKNGTLAIMVGGSEDVFLKVKPIFDILGSSVIL
QGDAGAGQHTKMVNQIAIASNMIGVTEAIIYAEAAGLNPSSVLDSISGGAAGSWSLANLIPRVLKDDFSPGFFIKHFIKD
MGIAISEAKQMGLELPGLTLAEKMYQTLAEQGLSEEGTQALIKYYR

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=290, Percent_Identity=30, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI40556376, Length=284, Percent_Identity=27.112676056338, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI145693186, Length=284, Percent_Identity=38.7323943661972, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI1786719, Length=281, Percent_Identity=40.2135231316726, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1790315, Length=288, Percent_Identity=30.9027777777778, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789092, Length=285, Percent_Identity=27.719298245614, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17557316, Length=278, Percent_Identity=25.1798561151079, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24655230, Length=282, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922568, Length=282, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28574115, Length=286, Percent_Identity=23.4265734265734, Blast_Score=101, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30152; Mature: 30152

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIGFVGTGVMGSSMAGHLLEAGYEVLVYTRTKTKAEDLLEKGALWIKTPGELANKVDI
CCCCCCEECCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCEEEECCHHHHHHHHH
LISMVGYPKDVEELYLGENGFLENLAVGTVAIDMTTSSPALAKKMAEFGREKGIGVLDAP
HHHHCCCCCCHHHHHCCCCCCHHHHEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECC
VSGGDIGAKNGTLAIMVGGSEDVFLKVKPIFDILGSSVILQGDAGAGQHTKMVNQIAIAS
CCCCCCCCCCCEEEEEECCCCCEEEEEEHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHH
NMIGVTEAIIYAEAAGLNPSSVLDSISGGAAGSWSLANLIPRVLKDDFSPGFFIKHFIKD
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
MGIAISEAKQMGLELPGLTLAEKMYQTLAEQGLSEEGTQALIKYYR
HCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MEKIGFVGTGVMGSSMAGHLLEAGYEVLVYTRTKTKAEDLLEKGALWIKTPGELANKVDI
CCCCCCEECCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCEEEECCHHHHHHHHH
LISMVGYPKDVEELYLGENGFLENLAVGTVAIDMTTSSPALAKKMAEFGREKGIGVLDAP
HHHHCCCCCCHHHHHCCCCCCHHHHEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECC
VSGGDIGAKNGTLAIMVGGSEDVFLKVKPIFDILGSSVILQGDAGAGQHTKMVNQIAIAS
CCCCCCCCCCCEEEEEECCCCCEEEEEEHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHH
NMIGVTEAIIYAEAAGLNPSSVLDSISGGAAGSWSLANLIPRVLKDDFSPGFFIKHFIKD
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
MGIAISEAKQMGLELPGLTLAEKMYQTLAEQGLSEEGTQALIKYYR
HCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]