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The map label for this gene is carA2 [H]

Identifier: 226223618

GI number: 226223618

Start: 1031813

End: 1033285

Strand: Reverse

Name: carA2 [H]

Synonym: Lm4b_01020

Alternate gene names: 226223618

Gene position: 1033285-1031813 (Counterclockwise)

Preceding gene: 226223619

Following gene: 226223616

Centisome position: 35.48

GC content: 35.51

Gene sequence:

>1473_bases
TTGAAATTGGAAAGTAAAAAGAAAATTGCCATTATAGGAGCTGGACCTGGTGGGTTGGCGGCCGGTATGCTATTAAGTCA
ACTAGGTTATCAAGTAAACATCTATGAAAAAAATGATCGAATTGGTGGAAGAACGGCGTTACACAGAATGGGTAAATACT
CATTTGATGTCGGACCTTCTGCACTCACCATGACGCATGTTTTGACTTCTCTTTTTATGGATTGTAATCGTAATATTTTG
GATTATGTTTCGCTTTTACCAATCAATCCAATTCATACGCTTTATTTTAAAGATATCACCTTCCCGCTTTATAGCGATCA
ATCTGAAACAAAAGCGGTTATCCAAACTTACTTTCCTGGCGAAGAAGCTGGTTTTGAACGCTTTATGAAAGAAAACACGA
AAAAAATGCTATATATTTCGCCGCTCAATCAATTTAATTATAGTTCTTTATTTGATTTTTTCCGCCCTACAACTTTGCGT
GCTATACCTAGTTTGACACTTGGACGATCGTTGATGGATGATTTAGCAAGATATTTTAATAGTAAGTACCTTCGCATCGC
TTTTTCCTTACAGATGCGGTATTTAGGCATGTCACCATGGGATATTCCAGCTGCTTATAGTATTATTCCTTTTTCCGAAT
ATTATTATGGAACTTTTCATCCAATTGGTGGGCAAAATAAAATTGTCGAAGCGATGCAACAAGTAGTTACAGAAAATAAA
GGGAAGTTTTTCTTCAATTCCGAAGTGACAGCATTTGAATCGAATGGTAAAGAAATAACTGGCGCTGTTCTTGCCAATGG
AAAAACAATTGAAGCAGATTATTATTTCACCAATTTAGATTTCATTTATTCTTTAACAAATGAGCATCCAGATAAATTGG
ACAATAAAGAATACTCCTCTTCTGCCTTTATCCTTTATCTTGGTTTAAAAACAGTGTTACCTTTTTCGCATCAATCAATT
ATTTTCCCTGAAAATTACCGAGAATTTGCGCATAATACAATGCATAAAAAAATCCTCTCTAAGGATATCGCTATACATTT
GACTAATCCTTCTGCAACTGATAATACAATGGCGCCAATTAATCATTCTAGTATTCGAATTATGGTTCCTGTGCCTAACA
ATACGAGTAATATTGATTGGAAAAAAGAAACACCTGCTTTTCGTCAATTAGTGCTAGAAACTATCAAAGAACGGTTAGAT
TTACCAGATTTAGAATCGCAAATTGAAGAAGAATATATCATTACACCGATAGACTGGGAAAAGAAATATCATGTTAAACA
CGGCGCGATCTTTGGTTTACAGCATTTATGGCGCCAGCACGGTTACTTACATCCCTCCAAAAAATCGCCTACATTCAAAA
ACTTATTTGTAATTGGTGCAGGCGCGATGTCGGGTAGCTCGCTTCCATTTATTATTGAAAATGCGCAAATCGCAACCCAG
AAATTTTTACAAAAAGAAAAGAAATCCGAGTAA

Upstream 100 bases:

>100_bases
ACAGAATAGTTTTTAAGCGCGCAAAAATAAAGCATTTCCGAGTGATAATGCTCTATATTTTTGCGTTTTTTATATGTATA
TAAACGGGGTAAAGGAGCGT

Downstream 100 bases:

>100_bases
TCATTCGGATTTCTTTTTAGGCTGCGCTTCTTTTTCCAAACTGAAAAAATAACAAGCAGTGAAAATAATATTAGAAACGA
TAATAATCGCAATAGATGTC

Product: phytoene dehydrogenase

Products: NA

Alternate protein names: Phytoene desaturase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MKLESKKKIAIIGAGPGGLAAGMLLSQLGYQVNIYEKNDRIGGRTALHRMGKYSFDVGPSALTMTHVLTSLFMDCNRNIL
DYVSLLPINPIHTLYFKDITFPLYSDQSETKAVIQTYFPGEEAGFERFMKENTKKMLYISPLNQFNYSSLFDFFRPTTLR
AIPSLTLGRSLMDDLARYFNSKYLRIAFSLQMRYLGMSPWDIPAAYSIIPFSEYYYGTFHPIGGQNKIVEAMQQVVTENK
GKFFFNSEVTAFESNGKEITGAVLANGKTIEADYYFTNLDFIYSLTNEHPDKLDNKEYSSSAFILYLGLKTVLPFSHQSI
IFPENYREFAHNTMHKKILSKDIAIHLTNPSATDNTMAPINHSSIRIMVPVPNNTSNIDWKKETPAFRQLVLETIKERLD
LPDLESQIEEEYIITPIDWEKKYHVKHGAIFGLQHLWRQHGYLHPSKKSPTFKNLFVIGAGAMSGSSLPFIIENAQIATQ
KFLQKEKKSE

Sequences:

>Translated_490_residues
MKLESKKKIAIIGAGPGGLAAGMLLSQLGYQVNIYEKNDRIGGRTALHRMGKYSFDVGPSALTMTHVLTSLFMDCNRNIL
DYVSLLPINPIHTLYFKDITFPLYSDQSETKAVIQTYFPGEEAGFERFMKENTKKMLYISPLNQFNYSSLFDFFRPTTLR
AIPSLTLGRSLMDDLARYFNSKYLRIAFSLQMRYLGMSPWDIPAAYSIIPFSEYYYGTFHPIGGQNKIVEAMQQVVTENK
GKFFFNSEVTAFESNGKEITGAVLANGKTIEADYYFTNLDFIYSLTNEHPDKLDNKEYSSSAFILYLGLKTVLPFSHQSI
IFPENYREFAHNTMHKKILSKDIAIHLTNPSATDNTMAPINHSSIRIMVPVPNNTSNIDWKKETPAFRQLVLETIKERLD
LPDLESQIEEEYIITPIDWEKKYHVKHGAIFGLQHLWRQHGYLHPSKKSPTFKNLFVIGAGAMSGSSLPFIIENAQIATQ
KFLQKEKKSE
>Mature_490_residues
MKLESKKKIAIIGAGPGGLAAGMLLSQLGYQVNIYEKNDRIGGRTALHRMGKYSFDVGPSALTMTHVLTSLFMDCNRNIL
DYVSLLPINPIHTLYFKDITFPLYSDQSETKAVIQTYFPGEEAGFERFMKENTKKMLYISPLNQFNYSSLFDFFRPTTLR
AIPSLTLGRSLMDDLARYFNSKYLRIAFSLQMRYLGMSPWDIPAAYSIIPFSEYYYGTFHPIGGQNKIVEAMQQVVTENK
GKFFFNSEVTAFESNGKEITGAVLANGKTIEADYYFTNLDFIYSLTNEHPDKLDNKEYSSSAFILYLGLKTVLPFSHQSI
IFPENYREFAHNTMHKKILSKDIAIHLTNPSATDNTMAPINHSSIRIMVPVPNNTSNIDWKKETPAFRQLVLETIKERLD
LPDLESQIEEEYIITPIDWEKKYHVKHGAIFGLQHLWRQHGYLHPSKKSPTFKNLFVIGAGAMSGSSLPFIIENAQIATQ
KFLQKEKKSE

Specific function: This enzyme converts phytoene into zeta-carotene via the intermediary of phytofluene by the symmetrical introduction of two double bonds at the C-11 and C-11' positions of phytoene [H]

COG id: COG1233

COG function: function code Q; Phytoene dehydrogenase and related proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid/retinoid oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002937
- InterPro:   IPR008150
- InterPro:   IPR014105 [H]

Pfam domain/function: PF01593 Amino_oxidase [H]

EC number: NA

Molecular weight: Translated: 55909; Mature: 55909

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLESKKKIAIIGAGPGGLAAGMLLSQLGYQVNIYEKNDRIGGRTALHRMGKYSFDVGPS
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCH
ALTMTHVLTSLFMDCNRNILDYVSLLPINPIHTLYFKDITFPLYSDQSETKAVIQTYFPG
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHEEEEEECCCEEECCCCHHHHHHEEECCC
EEAGFERFMKENTKKMLYISPLNQFNYSSLFDFFRPTTLRAIPSLTLGRSLMDDLARYFN
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHC
SKYLRIAFSLQMRYLGMSPWDIPAAYSIIPFSEYYYGTFHPIGGQNKIVEAMQQVVTENK
CCEEEEEEEEEEEECCCCCCCCCCCEEEEECHHHCCEEECCCCCCHHHHHHHHHHHHCCC
GKFFFNSEVTAFESNGKEITGAVLANGKTIEADYYFTNLDFIYSLTNEHPDKLDNKEYSS
CCEEECCCEEEEECCCCEEEEEEEECCEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCC
SAFILYLGLKTVLPFSHQSIIFPENYREFAHNTMHKKILSKDIAIHLTNPSATDNTMAPI
CEEEEEEEHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
NHSSIRIMVPVPNNTSNIDWKKETPAFRQLVLETIKERLDLPDLESQIEEEYIITPIDWE
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCEEECCCCCC
KKYHVKHGAIFGLQHLWRQHGYLHPSKKSPTFKNLFVIGAGAMSGSSLPFIIENAQIATQ
CEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCHHHHH
KFLQKEKKSE
HHHHHHHCCC
>Mature Secondary Structure
MKLESKKKIAIIGAGPGGLAAGMLLSQLGYQVNIYEKNDRIGGRTALHRMGKYSFDVGPS
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCH
ALTMTHVLTSLFMDCNRNILDYVSLLPINPIHTLYFKDITFPLYSDQSETKAVIQTYFPG
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHEEEEEECCCEEECCCCHHHHHHEEECCC
EEAGFERFMKENTKKMLYISPLNQFNYSSLFDFFRPTTLRAIPSLTLGRSLMDDLARYFN
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHC
SKYLRIAFSLQMRYLGMSPWDIPAAYSIIPFSEYYYGTFHPIGGQNKIVEAMQQVVTENK
CCEEEEEEEEEEEECCCCCCCCCCCEEEEECHHHCCEEECCCCCCHHHHHHHHHHHHCCC
GKFFFNSEVTAFESNGKEITGAVLANGKTIEADYYFTNLDFIYSLTNEHPDKLDNKEYSS
CCEEECCCEEEEECCCCEEEEEEEECCEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCC
SAFILYLGLKTVLPFSHQSIIFPENYREFAHNTMHKKILSKDIAIHLTNPSATDNTMAPI
CEEEEEEEHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
NHSSIRIMVPVPNNTSNIDWKKETPAFRQLVLETIKERLDLPDLESQIEEEYIITPIDWE
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCEEECCCCCC
KKYHVKHGAIFGLQHLWRQHGYLHPSKKSPTFKNLFVIGAGAMSGSSLPFIIENAQIATQ
CEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCHHHHH
KFLQKEKKSE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7588751 [H]