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The map label for this gene is ilvE [H]

Identifier: 226223596

GI number: 226223596

Start: 1010156

End: 1011175

Strand: Direct

Name: ilvE [H]

Synonym: Lm4b_00998

Alternate gene names: 226223596

Gene position: 1010156-1011175 (Clockwise)

Preceding gene: 226223593

Following gene: 226223597

Centisome position: 34.68

GC content: 38.73

Gene sequence:

>1020_bases
ATGAGTAAAGATATTGACTGGAGTAATTTAGGATTTAGTTATATTAAAACGGATAAGCGTTACATTTCTTATTGGAAAGA
CGGGGAATGGGATGAAGGTACGCTAACAGAAGATAATACGCTACATATTAGTGAGGGTTCAACAGCGCTTCATTACGGAC
AACAATGTTTTGAAGGTTTAAAAGCATATCGTTGTAAGGACGGTTCAATTAATCTTTTCCGCCCAGATCGGAATGCTGCT
CGTATTCAAAAAAGCTGTGAGCGTTTATTAATGCCTCACATCCCAACTGAAAAATTTATTGATGCTGTTATGCAAGTTGT
TCGTGCCAATGAAGAATTTGTACCGCCGTATGGAACAGGTGCAACACTTTATTTACGTCCATTTGTTATTGGTGTAGGTG
ACAATATTGGTGTTCATGCAGCCCCAGAGTATATTTTCTCTGTTTTCTGTTCACCAGTCGGCCCTTACTTCAAAGGTGGA
ATGGCGCCAACTAACTTTATTGTTTCCGATTTTGACCGTGCTGCTCCAAATGGTACGGGTGCCGCAAAAGTAGGCGGAAA
CTATGCTGCAAGTTTACTTCCAGGCCAGGCTGCAAAAGAACGCAATTTTGGTGATTGTATTTATTTAGATCCAGCTACTC
ATACAAAAATAGAAGAAGTTGGTTCTGCTAATTTCTTTGGTATTACAAAAGATGATAAATTTGTTACGCCGTATTCTCCT
TCTATTTTGCCAAGTATCACGAAGTATTCGTTACTTTATTTGGCAGAGCATCGTCTAGGTTTAGAAGCATTAGAAGGTGA
TGTATATATTGATAAATTAGATGAGTTTAAAGAAGCTGGAGCATGTGGAACGGCAGCGGTTATTACACCAATTGGTGGTA
TTCAAACCAAAGATGATTTCCATGTATTCCATAGTGAAACAGAAGTGGGGCCAGTAACGAAGCGTTTATTCGATGAACTA
TGTGGCATTCAATTTGGAGATGTAGAAGCTCCAGAAGGTTGGATTTATAAAGTGAAATAA

Upstream 100 bases:

>100_bases
AATTTCTTTTTCACGAGACATCACTGCAATAATGCTATTTAATATGCTATACTGAAACATACAATAATTCTGTTTTAATG
ATAGAAAGTGGGGATTTTGA

Downstream 100 bases:

>100_bases
TTTTAAACCGTCCGTTCAGCGGACGGTTTTTTTATGTACAAAATCAAAAGTCATTTCTGATTAATAAAAAGAAAATGACT
TTTTTATTGACATGATAGCA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSKDIDWSNLGFSYIKTDKRYISYWKDGEWDEGTLTEDNTLHISEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDRNAA
RIQKSCERLLMPHIPTEKFIDAVMQVVRANEEFVPPYGTGATLYLRPFVIGVGDNIGVHAAPEYIFSVFCSPVGPYFKGG
MAPTNFIVSDFDRAAPNGTGAAKVGGNYAASLLPGQAAKERNFGDCIYLDPATHTKIEEVGSANFFGITKDDKFVTPYSP
SILPSITKYSLLYLAEHRLGLEALEGDVYIDKLDEFKEAGACGTAAVITPIGGIQTKDDFHVFHSETEVGPVTKRLFDEL
CGIQFGDVEAPEGWIYKVK

Sequences:

>Translated_339_residues
MSKDIDWSNLGFSYIKTDKRYISYWKDGEWDEGTLTEDNTLHISEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDRNAA
RIQKSCERLLMPHIPTEKFIDAVMQVVRANEEFVPPYGTGATLYLRPFVIGVGDNIGVHAAPEYIFSVFCSPVGPYFKGG
MAPTNFIVSDFDRAAPNGTGAAKVGGNYAASLLPGQAAKERNFGDCIYLDPATHTKIEEVGSANFFGITKDDKFVTPYSP
SILPSITKYSLLYLAEHRLGLEALEGDVYIDKLDEFKEAGACGTAAVITPIGGIQTKDDFHVFHSETEVGPVTKRLFDEL
CGIQFGDVEAPEGWIYKVK
>Mature_338_residues
SKDIDWSNLGFSYIKTDKRYISYWKDGEWDEGTLTEDNTLHISEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDRNAAR
IQKSCERLLMPHIPTEKFIDAVMQVVRANEEFVPPYGTGATLYLRPFVIGVGDNIGVHAAPEYIFSVFCSPVGPYFKGGM
APTNFIVSDFDRAAPNGTGAAKVGGNYAASLLPGQAAKERNFGDCIYLDPATHTKIEEVGSANFFGITKDDKFVTPYSPS
ILPSITKYSLLYLAEHRLGLEALEGDVYIDKLDEFKEAGACGTAAVITPIGGIQTKDDFHVFHSETEVGPVTKRLFDELC
GIQFGDVEAPEGWIYKVK

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=341, Percent_Identity=32.8445747800587, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI38176287, Length=341, Percent_Identity=32.8445747800587, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI296010906, Length=341, Percent_Identity=32.8445747800587, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI50658084, Length=344, Percent_Identity=31.1046511627907, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI258614015, Length=299, Percent_Identity=31.7725752508361, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI296010902, Length=341, Percent_Identity=28.1524926686217, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI296010900, Length=341, Percent_Identity=28.1524926686217, Blast_Score=108, Evalue=5e-24,
Organism=Escherichia coli, GI48994963, Length=330, Percent_Identity=31.2121212121212, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17568601, Length=358, Percent_Identity=31.0055865921788, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17565728, Length=353, Percent_Identity=26.628895184136, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6322608, Length=342, Percent_Identity=32.1637426900585, Blast_Score=162, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6322002, Length=340, Percent_Identity=32.9411764705882, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24641779, Length=338, Percent_Identity=30.7692307692308, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37421; Mature: 37289

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDIDWSNLGFSYIKTDKRYISYWKDGEWDEGTLTEDNTLHISEGSTALHYGQQCFEGL
CCCCCCCCCCCHHHHCCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
KAYRCKDGSINLFRPDRNAARIQKSCERLLMPHIPTEKFIDAVMQVVRANEEFVPPYGTG
HHEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
ATLYLRPFVIGVGDNIGVHAAPEYIFSVFCSPVGPYFKGGMAPTNFIVSDFDRAAPNGTG
CEEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCC
AAKVGGNYAASLLPGQAAKERNFGDCIYLDPATHTKIEEVGSANFFGITKDDKFVTPYSP
CEECCCCEEECCCCCCHHCCCCCCCEEEECCCCCCHHHHCCCCCEEEECCCCCEECCCCC
SILPSITKYSLLYLAEHRLGLEALEGDVYIDKLDEFKEAGACGTAAVITPIGGIQTKDDF
CHHHHHHHHHHHHHHHHHCCHHHHCCCEEEHHHHHHHHCCCCCCEEEEECCCCCCCCCCE
HVFHSETEVGPVTKRLFDELCGIQFGDVEAPEGWIYKVK
EEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCCEEEEC
>Mature Secondary Structure 
SKDIDWSNLGFSYIKTDKRYISYWKDGEWDEGTLTEDNTLHISEGSTALHYGQQCFEGL
CCCCCCCCCCHHHHCCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
KAYRCKDGSINLFRPDRNAARIQKSCERLLMPHIPTEKFIDAVMQVVRANEEFVPPYGTG
HHEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
ATLYLRPFVIGVGDNIGVHAAPEYIFSVFCSPVGPYFKGGMAPTNFIVSDFDRAAPNGTG
CEEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCC
AAKVGGNYAASLLPGQAAKERNFGDCIYLDPATHTKIEEVGSANFFGITKDDKFVTPYSP
CEECCCCEEECCCCCCHHCCCCCCCEEEECCCCCCHHHHCCCCCEEEECCCCCEECCCCC
SILPSITKYSLLYLAEHRLGLEALEGDVYIDKLDEFKEAGACGTAAVITPIGGIQTKDDF
CHHHHHHHHHHHHHHHHHCCHHHHCCCEEEHHHHHHHHCCCCCCEEEEECCCCCCCCCCE
HVFHSETEVGPVTKRLFDELCGIQFGDVEAPEGWIYKVK
EEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]