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The map label for this gene is ywbA [H]

Identifier: 226223535

GI number: 226223535

Start: 951794

End: 953062

Strand: Direct

Name: ywbA [H]

Synonym: Lm4b_00935

Alternate gene names: 226223535

Gene position: 951794-953062 (Clockwise)

Preceding gene: 226223534

Following gene: 226223536

Centisome position: 32.68

GC content: 38.61

Gene sequence:

>1269_bases
ATGAACAACAAAGTGATGGATTTTATGACAAATAAATTTGCTCCAAAAGTAAATAAAGTTGTTAAAAATCCTTGGGTATC
AGCAATTCAAGATGCAATTATGTCTGCGCTTCCGCTCGTTTTTGTCGGTTCTTTAGTCACCATCGTTTCACTACTTAAAA
ATTTATTTCCGGGCATGCCTGATTTTTCGATGATAAGTAATTTTTCGTTTGGCATGTTTGGGTTAGTCGTGGCCTTTTTA
ATCCCATATTATCTGATGGAGAAAAAAGGAAATAGCAGCCAAAAATTAATTTCTGGCGCAACTGGACTTGTTCTATTTTT
AATGCTACTATTTCCAACTATTTCAGCAGACGGGGATGCCGTTTTCATTTTATCTAGATTTGGAGCGACAGGGATGTTCT
TATCCATTACAACCGGATTATTTGTTGGTTGTGTGATGAACTTTGCTGCGAAACGATCATTTTTCAGTGAAGATACACCG
ATTCCGGATTTTGTCGTTGGTTGGTTCAATAGCTTGCTACCAATTACATTTATTCTGATTGTTGGTTGGTTAATAACTGT
TCAATTTAATATTGATTTCTTTGAAGTGATTGTAGCAGTGTTTAGCCCGCTCGCGTCAATCGTACAATCTTATCCAGGCT
TTGTGCTTTCGGTTTTCATTCCAGCATTTCTTTATACATTCGGGATCTCTGGGTGGGTTATGATGCCCGCGATTTACCCG
GTTTATATGGCAGGACTCGCAGAAAACTCACAAGCTGTAGCAAATGGCGCAAGTGCATCGAACATCGCAACACAAGAAAC
TGTATACGCATTAATTTCAATCGGCGGGGTCGGCACAACCTTATCACTATCCATTATGATGCTCATTTTAAGTAAGTCTT
TACAGCTAAAAGCAATCGGAAAAGCAGTTATTGTCCCATCGATTTTTAATATCAACGAACCATTATTTTTCGGGGCACCA
ATTGCCTTCAACCCATACTTAATGATTCCAACGTGGATTAATGCTTTCTTGGTTCCAAGCATCGCCTATTTCGTTATGTC
GATGAACTTAGTAAGTATTCCCGCGCAATCGTTCTTGCTATGGTACATGCCTTATCCTGTAACGTCTTACCTTGCAACAC
AAGATTTCCGAGGTGTGATTGCTTGTTTAGCAATTATCGTCATCACATGGCTTGTCTACTTGCCGTTCTTTAAAGCATAC
GACAACTCCTTACTCAAACAAGAAAAATTAGATGCAATCGAAACAGAGAAAGAAATGGTAACAAATTGA

Upstream 100 bases:

>100_bases
GATAAAAATGTTTTAGTATCAATTATTCCGCAAGCAACTTATGGCACACTTAACGGTGAAAAAGCACTCGATCTCATTTT
AACTATGGAGGGATAACACG

Downstream 100 bases:

>100_bases
TTGGAGGAACTAAAATGTCAGAGCAAGACTATGTTGAAGAAACAGATAGCTTAAATGAACTATCCATGAATATATTAATC
CATGCTGGAAACGCAAGAAA

Product: phosphotransferase system enzyme IIC

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: Putative PTS system EIIC component [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNNKVMDFMTNKFAPKVNKVVKNPWVSAIQDAIMSALPLVFVGSLVTIVSLLKNLFPGMPDFSMISNFSFGMFGLVVAFL
IPYYLMEKKGNSSQKLISGATGLVLFLMLLFPTISADGDAVFILSRFGATGMFLSITTGLFVGCVMNFAAKRSFFSEDTP
IPDFVVGWFNSLLPITFILIVGWLITVQFNIDFFEVIVAVFSPLASIVQSYPGFVLSVFIPAFLYTFGISGWVMMPAIYP
VYMAGLAENSQAVANGASASNIATQETVYALISIGGVGTTLSLSIMMLILSKSLQLKAIGKAVIVPSIFNINEPLFFGAP
IAFNPYLMIPTWINAFLVPSIAYFVMSMNLVSIPAQSFLLWYMPYPVTSYLATQDFRGVIACLAIIVITWLVYLPFFKAY
DNSLLKQEKLDAIETEKEMVTN

Sequences:

>Translated_422_residues
MNNKVMDFMTNKFAPKVNKVVKNPWVSAIQDAIMSALPLVFVGSLVTIVSLLKNLFPGMPDFSMISNFSFGMFGLVVAFL
IPYYLMEKKGNSSQKLISGATGLVLFLMLLFPTISADGDAVFILSRFGATGMFLSITTGLFVGCVMNFAAKRSFFSEDTP
IPDFVVGWFNSLLPITFILIVGWLITVQFNIDFFEVIVAVFSPLASIVQSYPGFVLSVFIPAFLYTFGISGWVMMPAIYP
VYMAGLAENSQAVANGASASNIATQETVYALISIGGVGTTLSLSIMMLILSKSLQLKAIGKAVIVPSIFNINEPLFFGAP
IAFNPYLMIPTWINAFLVPSIAYFVMSMNLVSIPAQSFLLWYMPYPVTSYLATQDFRGVIACLAIIVITWLVYLPFFKAY
DNSLLKQEKLDAIETEKEMVTN
>Mature_422_residues
MNNKVMDFMTNKFAPKVNKVVKNPWVSAIQDAIMSALPLVFVGSLVTIVSLLKNLFPGMPDFSMISNFSFGMFGLVVAFL
IPYYLMEKKGNSSQKLISGATGLVLFLMLLFPTISADGDAVFILSRFGATGMFLSITTGLFVGCVMNFAAKRSFFSEDTP
IPDFVVGWFNSLLPITFILIVGWLITVQFNIDFFEVIVAVFSPLASIVQSYPGFVLSVFIPAFLYTFGISGWVMMPAIYP
VYMAGLAENSQAVANGASASNIATQETVYALISIGGVGTTLSLSIMMLILSKSLQLKAIGKAVIVPSIFNINEPLFFGAP
IAFNPYLMIPTWINAFLVPSIAYFVMSMNLVSIPAQSFLLWYMPYPVTSYLATQDFRGVIACLAIIVITWLVYLPFFKAY
DNSLLKQEKLDAIETEKEMVTN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=448, Percent_Identity=24.7767857142857, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 46374; Mature: 46374

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CC
>Mature Secondary Structure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CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]