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The map label for this gene is gatA1 [H]

Identifier: 226223467

GI number: 226223467

Start: 888023

End: 889870

Strand: Reverse

Name: gatA1 [H]

Synonym: Lm4b_00865

Alternate gene names: 226223467

Gene position: 889870-888023 (Counterclockwise)

Preceding gene: 226223476

Following gene: 226223461

Centisome position: 30.55

GC content: 37.39

Gene sequence:

>1848_bases
ATGAAAAGACGCATTTTATTAACAATCACCGCAGTTTGTACAGTTTTTAGTATCGTCGGTGGCTACGTATTCTTTACGAG
TCAACAAACTACTACATCAGAAAATAAAAGTGCTGTTTCTAAAAAAAAGCCACCAACCACAAAAAATGAAAAACCAAAAC
AACCACCAAACAAATTAACACTTGGCAAAAATAAAGAGAACTCCCATTTAGAAAAAAACTTCGGAGGTAAGCAATTACAA
GTCGAAGGTATAGACGACCGAGCTTTCCAAAGTTCGCTCAAAAAAAGAACCGAAATTAAAATGAAACCAGAAAAAGCACC
ACCAAAAAAACAGGACATTAAACCGGTAAAACCAATACCAAGCAAACCGGAAAAAGAAGAAGATTCATCTAGTCCCATTT
ATGAAAAAGCGCGTGTTATGAAGCCGATTAATAATCAATTAGATAAGTTAGATTTGGCTGTTCTTGAAGCAAAAGAACCA
CTTATCATTGGTGCTGATGTTACAAAACTCCAACAACTCATTGCGACAAAACAACTTAGCTACAAAGAATTAGCCGGTAT
TTATTTAAATCGAATCAAGAAATATGACCAAAATGGCCAAACTCTAAATGCAATAACCGAAATCAACCCCACTATTATTG
CGGAGGCAGAACAACTAGATAAAGATAATTCAGCCAACAAATCTGCTTTATACGGTATGCCAGTCCTTTTAAAAGATAAC
ATCGGCACAAAAGAACTTCCCACAAGTGCTGGGACTGTTGCTTTAAAAGATTGGGTAATCGGGGAGGACGCTACAATTGT
TGAAAATCTAAAAGCAAATGGTGCTTTAATTCTCGGAAAAACCAATATGTCTGAGTGGGCAGCTGGAATGGACGAAGAAC
TTCCAAACGGCTACTCAGGTAAAAAAGGCCAAAGCAAAAATCCTTATTCGTCGGACTTGGATCCCTCTGGTTCGAGTTCT
GGATCCGCTACTGGAGCAACAAGTGATTTTGCAGCAATTGCAATCGGTACAGAAACAAATGGTTCGATTATTATACCTGC
GTCTGCTCAATCTGCTGTTGGGTATAAGCCATCTCAAGGTTTAATAAATAACAAAGGTATTATTCCCTTATCAAGCAGAT
TTGATACACCCGGACCACTAACAAGAACTGTTAATGATGCCTATTTGACGACAAATGCTCTAATAAATACTACTTCCAAT
CCACCGCTTTCTACGGATTCTCTTCAAGGTAAACGTATCGGTCTTTTAGCGGATGGCGAAAGCAATGAAGAAACAGCAGT
TATAAAAAAAATAAAATATGATTTGAAAAAAGCAGGTGCTATTATCATTGAAGGTGTTGCAGTAGGTGAATTTGAACAGT
TAGATACTGATTATGCCTTATTACTAAATGCTGACTTTAAACATGATTTGAATCAGTTTTTGAATGTGAACAACGCTCCT
ATGACGACGTTAGAATCAATTATTCAATTTAATCAGACCAACCCAGCGAGAAATATGAAATACGGCCAATCCGAACTTGT
CAAATCACAACAAAGCACAACTACGAAGCTACAAGCAGACAGTTTGGCGAGTAATTTGATTCAGGAGGCGCAAAACGAAC
TTGACTCTATGCTTCAAAAAGATAAACTGGATGCGGTCGTAACAATTGGCATGGGAGGTTCCGTGACGTTTCTAGCACCC
ATTGCAGGAAACCCAGAACTGACTATCCCAGCTGGCTATGATGAAGAAACAAATCAACCGATTAGTTTAACTTTTATTAC
AGCTAGAAATAGTGATACAACCTTATTAAATATGGGGTATTCCTATGAACAACAATCGAAAAACAGAAAAAGTCCTAATT
TAAAGTAA

Upstream 100 bases:

>100_bases
CCCTTTTTAAAAAATTTTTTAGTTTTCACAAAATTGTCACTGATTTACTTTTTTTGATGCTATATAATTAAATTACATAT
TTAAACATCGGAGGAATCGC

Downstream 100 bases:

>100_bases
AAAAAAGCAAGATGCTTTTGGAAATTCCAGAGTCATCTTGCTTTTTGTTTATAATACTTTATCTAAGAAACTAATCGTTC
TTTCGTTGGTTGGGTTATCA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 1 [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MKRRILLTITAVCTVFSIVGGYVFFTSQQTTTSENKSAVSKKKPPTTKNEKPKQPPNKLTLGKNKENSHLEKNFGGKQLQ
VEGIDDRAFQSSLKKRTEIKMKPEKAPPKKQDIKPVKPIPSKPEKEEDSSSPIYEKARVMKPINNQLDKLDLAVLEAKEP
LIIGADVTKLQQLIATKQLSYKELAGIYLNRIKKYDQNGQTLNAITEINPTIIAEAEQLDKDNSANKSALYGMPVLLKDN
IGTKELPTSAGTVALKDWVIGEDATIVENLKANGALILGKTNMSEWAAGMDEELPNGYSGKKGQSKNPYSSDLDPSGSSS
GSATGATSDFAAIAIGTETNGSIIIPASAQSAVGYKPSQGLINNKGIIPLSSRFDTPGPLTRTVNDAYLTTNALINTTSN
PPLSTDSLQGKRIGLLADGESNEETAVIKKIKYDLKKAGAIIIEGVAVGEFEQLDTDYALLLNADFKHDLNQFLNVNNAP
MTTLESIIQFNQTNPARNMKYGQSELVKSQQSTTTKLQADSLASNLIQEAQNELDSMLQKDKLDAVVTIGMGGSVTFLAP
IAGNPELTIPAGYDEETNQPISLTFITARNSDTTLLNMGYSYEQQSKNRKSPNLK

Sequences:

>Translated_615_residues
MKRRILLTITAVCTVFSIVGGYVFFTSQQTTTSENKSAVSKKKPPTTKNEKPKQPPNKLTLGKNKENSHLEKNFGGKQLQ
VEGIDDRAFQSSLKKRTEIKMKPEKAPPKKQDIKPVKPIPSKPEKEEDSSSPIYEKARVMKPINNQLDKLDLAVLEAKEP
LIIGADVTKLQQLIATKQLSYKELAGIYLNRIKKYDQNGQTLNAITEINPTIIAEAEQLDKDNSANKSALYGMPVLLKDN
IGTKELPTSAGTVALKDWVIGEDATIVENLKANGALILGKTNMSEWAAGMDEELPNGYSGKKGQSKNPYSSDLDPSGSSS
GSATGATSDFAAIAIGTETNGSIIIPASAQSAVGYKPSQGLINNKGIIPLSSRFDTPGPLTRTVNDAYLTTNALINTTSN
PPLSTDSLQGKRIGLLADGESNEETAVIKKIKYDLKKAGAIIIEGVAVGEFEQLDTDYALLLNADFKHDLNQFLNVNNAP
MTTLESIIQFNQTNPARNMKYGQSELVKSQQSTTTKLQADSLASNLIQEAQNELDSMLQKDKLDAVVTIGMGGSVTFLAP
IAGNPELTIPAGYDEETNQPISLTFITARNSDTTLLNMGYSYEQQSKNRKSPNLK
>Mature_615_residues
MKRRILLTITAVCTVFSIVGGYVFFTSQQTTTSENKSAVSKKKPPTTKNEKPKQPPNKLTLGKNKENSHLEKNFGGKQLQ
VEGIDDRAFQSSLKKRTEIKMKPEKAPPKKQDIKPVKPIPSKPEKEEDSSSPIYEKARVMKPINNQLDKLDLAVLEAKEP
LIIGADVTKLQQLIATKQLSYKELAGIYLNRIKKYDQNGQTLNAITEINPTIIAEAEQLDKDNSANKSALYGMPVLLKDN
IGTKELPTSAGTVALKDWVIGEDATIVENLKANGALILGKTNMSEWAAGMDEELPNGYSGKKGQSKNPYSSDLDPSGSSS
GSATGATSDFAAIAIGTETNGSIIIPASAQSAVGYKPSQGLINNKGIIPLSSRFDTPGPLTRTVNDAYLTTNALINTTSN
PPLSTDSLQGKRIGLLADGESNEETAVIKKIKYDLKKAGAIIIEGVAVGEFEQLDTDYALLLNADFKHDLNQFLNVNNAP
MTTLESIIQFNQTNPARNMKYGQSELVKSQQSTTTKLQADSLASNLIQEAQNELDSMLQKDKLDAVVTIGMGGSVTFLAP
IAGNPELTIPAGYDEETNQPISLTFITARNSDTTLLNMGYSYEQQSKNRKSPNLK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=251, Percent_Identity=33.0677290836653, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI195972892, Length=244, Percent_Identity=29.5081967213115, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=189, Percent_Identity=31.2169312169312, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=298, Percent_Identity=26.510067114094, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=186, Percent_Identity=35.4838709677419, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319685, Length=158, Percent_Identity=32.9113924050633, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320448, Length=247, Percent_Identity=27.1255060728745, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648113, Length=214, Percent_Identity=35.5140186915888, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648435, Length=287, Percent_Identity=26.8292682926829, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648437, Length=287, Percent_Identity=26.8292682926829, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45550774, Length=288, Percent_Identity=27.7777777777778, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24648441, Length=217, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24648439, Length=217, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24644968, Length=247, Percent_Identity=26.3157894736842, Blast_Score=73, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21356731, Length=327, Percent_Identity=23.2415902140673, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI161078093, Length=237, Percent_Identity=26.5822784810127, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 66687; Mature: 66687

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRILLTITAVCTVFSIVGGYVFFTSQQTTTSENKSAVSKKKPPTTKNEKPKQPPNKLT
CCCEEEEHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEE
LGKNKENSHLEKNFGGKQLQVEGIDDRAFQSSLKKRTEIKMKPEKAPPKKQDIKPVKPIP
CCCCCCCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCCC
SKPEKEEDSSSPIYEKARVMKPINNQLDKLDLAVLEAKEPLIIGADVTKLQQLIATKQLS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCEEECCCHHHHHHHHHHHHCC
YKELAGIYLNRIKKYDQNGQTLNAITEINPTIIAEAEQLDKDNSANKSALYGMPVLLKDN
HHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCEEECHHHCCCCCCCCCCCCCCCCEEEECC
IGTKELPTSAGTVALKDWVIGEDATIVENLKANGALILGKTNMSEWAAGMDEELPNGYSG
CCCCCCCCCCCEEEEEEEEECCCCHHHHHCCCCCEEEEECCCHHHHHCCCHHHCCCCCCC
KKGQSKNPYSSDLDPSGSSSGSATGATSDFAAIAIGTETNGSIIIPASAQSAVGYKPSQG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCC
LINNKGIIPLSSRFDTPGPLTRTVNDAYLTTNALINTTSNPPLSTDSLQGKRIGLLADGE
CCCCCCEEEECCCCCCCCCCEECCCCCEEEECEEEECCCCCCCCCCCCCCCEEEEEECCC
SNEETAVIKKIKYDLKKAGAIIIEGVAVGEFEQLDTDYALLLNADFKHDLNQFLNVNNAP
CCCHHHHHHHHHHHHHHCCEEEEEEEECCCHHHCCCCEEEEEECCHHHHHHHHHCCCCCC
MTTLESIIQFNQTNPARNMKYGQSELVKSQQSTTTKLQADSLASNLIQEAQNELDSMLQK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKLDAVVTIGMGGSVTFLAPIAGNPELTIPAGYDEETNQPISLTFITARNSDTTLLNMGY
CCCCEEEEEECCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEECCCCCEEEECCC
SYEQQSKNRKSPNLK
CHHHHHHCCCCCCCC
>Mature Secondary Structure
MKRRILLTITAVCTVFSIVGGYVFFTSQQTTTSENKSAVSKKKPPTTKNEKPKQPPNKLT
CCCEEEEHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEE
LGKNKENSHLEKNFGGKQLQVEGIDDRAFQSSLKKRTEIKMKPEKAPPKKQDIKPVKPIP
CCCCCCCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCCC
SKPEKEEDSSSPIYEKARVMKPINNQLDKLDLAVLEAKEPLIIGADVTKLQQLIATKQLS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCEEECCCHHHHHHHHHHHHCC
YKELAGIYLNRIKKYDQNGQTLNAITEINPTIIAEAEQLDKDNSANKSALYGMPVLLKDN
HHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCEEECHHHCCCCCCCCCCCCCCCCEEEECC
IGTKELPTSAGTVALKDWVIGEDATIVENLKANGALILGKTNMSEWAAGMDEELPNGYSG
CCCCCCCCCCCEEEEEEEEECCCCHHHHHCCCCCEEEEECCCHHHHHCCCHHHCCCCCCC
KKGQSKNPYSSDLDPSGSSSGSATGATSDFAAIAIGTETNGSIIIPASAQSAVGYKPSQG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCC
LINNKGIIPLSSRFDTPGPLTRTVNDAYLTTNALINTTSNPPLSTDSLQGKRIGLLADGE
CCCCCCEEEECCCCCCCCCCEECCCCCEEEECEEEECCCCCCCCCCCCCCCEEEEEECCC
SNEETAVIKKIKYDLKKAGAIIIEGVAVGEFEQLDTDYALLLNADFKHDLNQFLNVNNAP
CCCHHHHHHHHHHHHHHCCEEEEEEEECCCHHHCCCCEEEEEECCHHHHHHHHHCCCCCC
MTTLESIIQFNQTNPARNMKYGQSELVKSQQSTTTKLQADSLASNLIQEAQNELDSMLQK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKLDAVVTIGMGGSVTFLAPIAGNPELTIPAGYDEETNQPISLTFITARNSDTTLLNMGY
CCCCEEEEEECCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEECCCCCEEEECCC
SYEQQSKNRKSPNLK
CHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]