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The map label for this gene is tetA [H]

Identifier: 226223457

GI number: 226223457

Start: 871020

End: 872231

Strand: Reverse

Name: tetA [H]

Synonym: Lm4b_00855

Alternate gene names: 226223457

Gene position: 872231-871020 (Counterclockwise)

Preceding gene: 226223458

Following gene: 226223454

Centisome position: 29.95

GC content: 42.24

Gene sequence:

>1212_bases
ATGTCTTCAACTAAATCTATTATTAATAAGAAAACTTTACTTTTCGGTCTTATATCTGTCTTCCTTTGTGGGATGGGTTT
TAGTATTATTATGCCCGTTGTCCCGTTTCTCGTAACACCTTACGTAACAAATGCTAGTGATCAAGCGCTTATGGTGACAC
TGCTCACATCTGTTTATGCGCTTTGCGTGTTCTTTGCTGCGCCTGGTCTTGGTGCTTTAAGTGACAGATTTGGCAGACGA
CCAGTGTTACTCATTTGTTTTATCGGCTCTGCGATTGGTTACTTTATTTTCGGTCTTGGTGGGGCGCTTTGGGTGCTCTT
TCTTGGGCGAATTATTGAAGGAATCACTGGTGGTAGCATAAGTACACTATTTGCTTTCTTTGCTGATATTACGCCTCAAA
AGGAGCGGACGAAATACTTTGGTTGGGTTAGCGCTGCAGCTGGTGCGGGTGCTGCGCTTGGTCCGGCTTTCGGTGGATTG
CTTGCCCACTTTGGTTACGCAATGCCATTTTTCTTTGGAGCAGCGATTACTTTCATCAACTTTTTATTCGGTTATTTCTA
CATGCCTGAGAGTTTAGAAGAAGCAAATCGTTTAAAACGAATTCCGCTTATGCGACTCAATCCGTTTTCCCAGCTGCTTA
ATATTCTGACCATCAAAAACTTAGGACGTTTACTGATTGCTGCATTTTTCATTTGGGTTCCTAATGGTTCCTTGCAAGCT
GTCATGTCTCAATTCGCCATTGATAGTTTCAGTTGGAAGCCAGCTTTAATTGGGATGATGTTTTCCATTATGGGAATTCA
AGATATCTTGTCGCAAGCTTTTGTTATGCCAAAATTACTACTCAAGTTAACGGACAAACAAATAGCTATTTTGGGGATGA
TTGCTGAGATTATTGGCTATTTGCTTATTGCAGCTTCCTCGATTTTCACACTTGCGCCGCTACTCGTTATCGGAATGTTC
GTTTTCGGTTTTGGCGACTCGATTTTCGGACCTTCGTTTAACGGCATGGTTTCAAAATCTGCTAGCGCTAGCGAACAAGG
TCGGATTCAAGGAGGTAGCCAAGCCATTCAGTCGGTGGCGCGTATTATCGGACCCGTTATCGGTGGGCAACTTTATATTA
CGCTTGGCCATGCCGCTCCAGCTATTATGGGTGTCCTTTTAATCACTGTTGCAATTTTTATTCTTTATAAAAAGACTTCT
TTAGCAAAATAA

Upstream 100 bases:

>100_bases
GGTTGTTTTTTATTGTTTTATTTTTGTTGACAAGGAAACAAAAACGAGTTAAGCTAATTATGTTTCCTAGGACACTAATT
ACAATCGATAGGAGTATTAC

Downstream 100 bases:

>100_bases
TGTTTTTCGTATTTAACAAACCATTTTGTAATGAAAAAGGTATCCTTATACTATTAATTAGGATACCTTTTTAGTAATTA
GTTTATCTTCACTCATTACT

Product: Tetracycline resistance protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: TetA(A) [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSSTKSIINKKTLLFGLISVFLCGMGFSIIMPVVPFLVTPYVTNASDQALMVTLLTSVYALCVFFAAPGLGALSDRFGRR
PVLLICFIGSAIGYFIFGLGGALWVLFLGRIIEGITGGSISTLFAFFADITPQKERTKYFGWVSAAAGAGAALGPAFGGL
LAHFGYAMPFFFGAAITFINFLFGYFYMPESLEEANRLKRIPLMRLNPFSQLLNILTIKNLGRLLIAAFFIWVPNGSLQA
VMSQFAIDSFSWKPALIGMMFSIMGIQDILSQAFVMPKLLLKLTDKQIAILGMIAEIIGYLLIAASSIFTLAPLLVIGMF
VFGFGDSIFGPSFNGMVSKSASASEQGRIQGGSQAIQSVARIIGPVIGGQLYITLGHAAPAIMGVLLITVAIFILYKKTS
LAK

Sequences:

>Translated_403_residues
MSSTKSIINKKTLLFGLISVFLCGMGFSIIMPVVPFLVTPYVTNASDQALMVTLLTSVYALCVFFAAPGLGALSDRFGRR
PVLLICFIGSAIGYFIFGLGGALWVLFLGRIIEGITGGSISTLFAFFADITPQKERTKYFGWVSAAAGAGAALGPAFGGL
LAHFGYAMPFFFGAAITFINFLFGYFYMPESLEEANRLKRIPLMRLNPFSQLLNILTIKNLGRLLIAAFFIWVPNGSLQA
VMSQFAIDSFSWKPALIGMMFSIMGIQDILSQAFVMPKLLLKLTDKQIAILGMIAEIIGYLLIAASSIFTLAPLLVIGMF
VFGFGDSIFGPSFNGMVSKSASASEQGRIQGGSQAIQSVARIIGPVIGGQLYITLGHAAPAIMGVLLITVAIFILYKKTS
LAK
>Mature_402_residues
SSTKSIINKKTLLFGLISVFLCGMGFSIIMPVVPFLVTPYVTNASDQALMVTLLTSVYALCVFFAAPGLGALSDRFGRRP
VLLICFIGSAIGYFIFGLGGALWVLFLGRIIEGITGGSISTLFAFFADITPQKERTKYFGWVSAAAGAGAALGPAFGGLL
AHFGYAMPFFFGAAITFINFLFGYFYMPESLEEANRLKRIPLMRLNPFSQLLNILTIKNLGRLLIAAFFIWVPNGSLQAV
MSQFAIDSFSWKPALIGMMFSIMGIQDILSQAFVMPKLLLKLTDKQIAILGMIAEIIGYLLIAASSIFTLAPLLVIGMFV
FGFGDSIFGPSFNGMVSKSASASEQGRIQGGSQAIQSVARIIGPVIGGQLYITLGHAAPAIMGVLLITVAIFILYKKTSL
AK

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI39753965, Length=340, Percent_Identity=26.1764705882353, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI94721311, Length=357, Percent_Identity=24.3697478991597, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI217416366, Length=417, Percent_Identity=24.4604316546763, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI225703104, Length=359, Percent_Identity=22.8412256267409, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI225703102, Length=359, Percent_Identity=22.8412256267409, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1787291, Length=406, Percent_Identity=26.1083743842365, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17551570, Length=430, Percent_Identity=23.0232558139535, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71996331, Length=354, Percent_Identity=23.1638418079096, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17738085, Length=358, Percent_Identity=25.6983240223464, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221513297, Length=204, Percent_Identity=27.4509803921569, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21357569, Length=374, Percent_Identity=24.0641711229947, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24656463, Length=370, Percent_Identity=23.7837837837838, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24656468, Length=370, Percent_Identity=23.7837837837838, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI161080926, Length=370, Percent_Identity=23.7837837837838, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI161080924, Length=370, Percent_Identity=23.7837837837838, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43263; Mature: 43132

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTKSIINKKTLLFGLISVFLCGMGFSIIMPVVPFLVTPYVTNASDQALMVTLLTSVYA
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LCVFFAAPGLGALSDRFGRRPVLLICFIGSAIGYFIFGLGGALWVLFLGRIIEGITGGSI
HHHHHHCCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
STLFAFFADITPQKERTKYFGWVSAAAGAGAALGPAFGGLLAHFGYAMPFFFGAAITFIN
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLFGYFYMPESLEEANRLKRIPLMRLNPFSQLLNILTIKNLGRLLIAAFFIWVPNGSLQA
HHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VMSQFAIDSFSWKPALIGMMFSIMGIQDILSQAFVMPKLLLKLTDKQIAILGMIAEIIGY
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LLIAASSIFTLAPLLVIGMFVFGFGDSIFGPSFNGMVSKSASASEQGRIQGGSQAIQSVA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHH
RIIGPVIGGQLYITLGHAAPAIMGVLLITVAIFILYKKTSLAK
HHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSTKSIINKKTLLFGLISVFLCGMGFSIIMPVVPFLVTPYVTNASDQALMVTLLTSVYA
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LCVFFAAPGLGALSDRFGRRPVLLICFIGSAIGYFIFGLGGALWVLFLGRIIEGITGGSI
HHHHHHCCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
STLFAFFADITPQKERTKYFGWVSAAAGAGAALGPAFGGLLAHFGYAMPFFFGAAITFIN
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLFGYFYMPESLEEANRLKRIPLMRLNPFSQLLNILTIKNLGRLLIAAFFIWVPNGSLQA
HHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VMSQFAIDSFSWKPALIGMMFSIMGIQDILSQAFVMPKLLLKLTDKQIAILGMIAEIIGY
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LLIAASSIFTLAPLLVIGMFVFGFGDSIFGPSFNGMVSKSASASEQGRIQGGSQAIQSVA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHH
RIIGPVIGGQLYITLGHAAPAIMGVLLITVAIFILYKKTSLAK
HHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312499; 6310527 [H]