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The map label for this gene is manX [H]

Identifier: 226223401

GI number: 226223401

Start: 806596

End: 807030

Strand: Reverse

Name: manX [H]

Synonym: Lm4b_00799

Alternate gene names: 226223401

Gene position: 807030-806596 (Counterclockwise)

Preceding gene: 226223402

Following gene: 226223400

Centisome position: 27.71

GC content: 39.54

Gene sequence:

>435_bases
ATGCCAGTTGGAATTATCATTGGCACACATGGTGAAGCTGCGCGTGAATTGTTGAAATCAACTGAAATGATTATCGGCAA
ACAGGACAATGTAGAATTTATTACTTTTGTTCCTGGAGAAAACACTGATACGCTTATTACCAAATACAAAGAAAAACTAG
ACAAATTGGATACTTCTGAAGGCGTTATTTTTATGGTTGATTTATTTGGCGGCAGTCCTTATAACGCATCTAGCCAAATC
GCCCTTCCAGAAGACAATATGGATGTTGTCACGGGGGTCAATATTCCCATGTTACTTGAAACGCTCTCAATGCGGGCTGC
AAGTAATCAAAAAGATCTAGTTGAAACAGCTCTTACAGCAGGATCAGGTGGCGTTAAGTCACTCAAACAATCATTACCAA
AAATAGAAACCGAAGTCGGGGAGGACGATTTATAA

Upstream 100 bases:

>100_bases
CTTCTATTAGCAAAGGAGGTTGTTTAATACAGAAATGTTGGCACAGTTTTTGCGTTAGTATTAATGGATGATTATATCCA
CGCTTAAGGAGGAGATACCT

Downstream 100 bases:

>100_bases
TGGAAATTCGCTTAGCACGTATTGATGATCGCTTAATTCATGGTCAAGTAGCAACCGTTTGGACAAAAGAAACGCAGGTA
GAACGTATCATCGTTATTAG

Product: mannose-specific phosphotransferase system (PTS) component IIA

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MPVGIIIGTHGEAARELLKSTEMIIGKQDNVEFITFVPGENTDTLITKYKEKLDKLDTSEGVIFMVDLFGGSPYNASSQI
ALPEDNMDVVTGVNIPMLLETLSMRAASNQKDLVETALTAGSGGVKSLKQSLPKIETEVGEDDL

Sequences:

>Translated_144_residues
MPVGIIIGTHGEAARELLKSTEMIIGKQDNVEFITFVPGENTDTLITKYKEKLDKLDTSEGVIFMVDLFGGSPYNASSQI
ALPEDNMDVVTGVNIPMLLETLSMRAASNQKDLVETALTAGSGGVKSLKQSLPKIETEVGEDDL
>Mature_143_residues
PVGIIIGTHGEAARELLKSTEMIIGKQDNVEFITFVPGENTDTLITKYKEKLDKLDTSEGVIFMVDLFGGSPYNASSQIA
LPEDNMDVVTGVNIPMLLETLSMRAASNQKDLVETALTAGSGGVKSLKQSLPKIETEVGEDDL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=130, Percent_Identity=53.8461538461538, Blast_Score=144, Evalue=3e-36,
Organism=Escherichia coli, GI1786321, Length=132, Percent_Identity=25.7575757575758, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 15488; Mature: 15357

Theoretical pI: Translated: 4.15; Mature: 4.15

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVGIIIGTHGEAARELLKSTEMIIGKQDNVEFITFVPGENTDTLITKYKEKLDKLDTSE
CCEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
GVIFMVDLFGGSPYNASSQIALPEDNMDVVTGVNIPMLLETLSMRAASNQKDLVETALTA
CEEEEEEECCCCCCCCCCEEECCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHC
GSGGVKSLKQSLPKIETEVGEDDL
CCCCHHHHHHHCCHHHHCCCCCCC
>Mature Secondary Structure 
PVGIIIGTHGEAARELLKSTEMIIGKQDNVEFITFVPGENTDTLITKYKEKLDKLDTSE
CEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
GVIFMVDLFGGSPYNASSQIALPEDNMDVVTGVNIPMLLETLSMRAASNQKDLVETALTA
CEEEEEEECCCCCCCCCCEEECCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHC
GSGGVKSLKQSLPKIETEVGEDDL
CCCCHHHHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]