Click here to switch to the map view.

The map label for this gene is ddeIM [H]

Identifier: 226223282

GI number: 226223282

Start: 697249

End: 698418

Strand: Direct

Name: ddeIM [H]

Synonym: Lm4b_00679

Alternate gene names: 226223282

Gene position: 697249-698418 (Clockwise)

Preceding gene: 226223281

Following gene: 226223283

Centisome position: 23.94

GC content: 32.65

Gene sequence:

>1170_bases
ATGATTAGAGTTGTAGATTTATTTTCTGGTGCAGGGGGTTTGACATTAGGATTTCAGAATAGAATTATAAATAACACCTT
TTTACCTATGTCTGATTATAATATTTTATTTGCTAATGAAGTGGATAAACACGCATCTGAAGCCTTCTCTCTAAATTTCC
CACAAATTCCAATGTTAAATTGTAGTATAACGGAACTAACCGAGGATTATTTAGATAATCATGAGATTGAGTATTCTAAT
ATTGATTTAGTAATAGGTGGCCCTCCCTGCCAATCCTTCAGTACAGTTGGTAGGAGGCAATATGATGAAAAGGCTACGAT
GTATAAGGAATACAGAAGAATGCTTTCCTTCTTACAGCCTAAAGTATTCCTTTTTGAAAATGTGACAGGTCTTTTAACGA
TGAAAAATGATGAAGGGGAACCTGTACTTGAAGATATCAGAAAAGAATTTGGCGATTTTTCTGACTTTGAAATTGATTTA
ACCTATGAAATTAGAGAAACAGTTCTAAATGCAAAACATTTTGGTGTCCCTCAGAATAGAGAGAGAGTATTTTTACTAGG
GATAAGAAAGGATTTAGAAATTAAACAAAATTGGGAATTTCCAGAACGATTATTATTTGAGGATGATTTTTTAACATTAG
AAGATGCTATAGGAGATTTACCTGCGTTGAGAAATGGTGAAAGGAAGGATGCTTACGATGGAGATCCGTATACTCCTTAT
CAAAATTTAATGAGGAATAATTCGACTATATTAATGGACCATTTCAATGGGTTAAACGGTGAAAAAATGTTGAGAATTAT
GGAAACAGTTATTCCGGGAGAGGGGAAAAAATATATAAATGAGTTAGTTGAATCTGGACAATTAGATTCAAAGTATTACC
TAACATCTGGTTATAATAATACATATAGTAAACTTTGGTGGGATAGACCTAGTTCAACAATTACTAATAATTTATCCACG
CCATCTTCTTTCAGATGTATACATCCAATACAAAATAGAGCATTAACCCCTCGAGAAGGAGCTAGGATACAGTCTTTTCC
GGATACATTTCAATTTGTTGGCAGTAAGGAAAAAATTAATAGCCAAATTGGTAATGCTGTACCTCCTTTATTAGCGATTG
TTATTGCACAAGAAATATATAATTTTTTTGAAGAGAACAATTGGATATAG

Upstream 100 bases:

>100_bases
AAGATGAAAAAATGAGATATACTAAGATCAATAATTTATATCAATTTTTGTGGGTTTTTGATAAAATGTATCGTATGAGA
AGATTTAGGAGGACATAATT

Downstream 100 bases:

>100_bases
AAGATTTTATTGAAATAACAAGAGGTGAAAACATGAACGAAAAACAAAATGAAATCCCATTTGATTTTTCTTATTATGCT
TTACATTTATTAGGTAAAGG

Product: type II DNA modification enzyme (methyltransferase)

Products: NA

Alternate protein names: M.DdeI; Cytosine-specific methyltransferase DdeI [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MIRVVDLFSGAGGLTLGFQNRIINNTFLPMSDYNILFANEVDKHASEAFSLNFPQIPMLNCSITELTEDYLDNHEIEYSN
IDLVIGGPPCQSFSTVGRRQYDEKATMYKEYRRMLSFLQPKVFLFENVTGLLTMKNDEGEPVLEDIRKEFGDFSDFEIDL
TYEIRETVLNAKHFGVPQNRERVFLLGIRKDLEIKQNWEFPERLLFEDDFLTLEDAIGDLPALRNGERKDAYDGDPYTPY
QNLMRNNSTILMDHFNGLNGEKMLRIMETVIPGEGKKYINELVESGQLDSKYYLTSGYNNTYSKLWWDRPSSTITNNLST
PSSFRCIHPIQNRALTPREGARIQSFPDTFQFVGSKEKINSQIGNAVPPLLAIVIAQEIYNFFEENNWI

Sequences:

>Translated_389_residues
MIRVVDLFSGAGGLTLGFQNRIINNTFLPMSDYNILFANEVDKHASEAFSLNFPQIPMLNCSITELTEDYLDNHEIEYSN
IDLVIGGPPCQSFSTVGRRQYDEKATMYKEYRRMLSFLQPKVFLFENVTGLLTMKNDEGEPVLEDIRKEFGDFSDFEIDL
TYEIRETVLNAKHFGVPQNRERVFLLGIRKDLEIKQNWEFPERLLFEDDFLTLEDAIGDLPALRNGERKDAYDGDPYTPY
QNLMRNNSTILMDHFNGLNGEKMLRIMETVIPGEGKKYINELVESGQLDSKYYLTSGYNNTYSKLWWDRPSSTITNNLST
PSSFRCIHPIQNRALTPREGARIQSFPDTFQFVGSKEKINSQIGNAVPPLLAIVIAQEIYNFFEENNWI
>Mature_389_residues
MIRVVDLFSGAGGLTLGFQNRIINNTFLPMSDYNILFANEVDKHASEAFSLNFPQIPMLNCSITELTEDYLDNHEIEYSN
IDLVIGGPPCQSFSTVGRRQYDEKATMYKEYRRMLSFLQPKVFLFENVTGLLTMKNDEGEPVLEDIRKEFGDFSDFEIDL
TYEIRETVLNAKHFGVPQNRERVFLLGIRKDLEIKQNWEFPERLLFEDDFLTLEDAIGDLPALRNGERKDAYDGDPYTPY
QNLMRNNSTILMDHFNGLNGEKMLRIMETVIPGEGKKYINELVESGQLDSKYYLTSGYNNTYSKLWWDRPSSTITNNLST
PSSFRCIHPIQNRALTPREGARIQSFPDTFQFVGSKEKINSQIGNAVPPLLAIVIAQEIYNFFEENNWI

Specific function: This methylase recognizes the double-stranded sequence CTNAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the DdeI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=223, Percent_Identity=26.457399103139, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 44887; Mature: 44887

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVVDLFSGAGGLTLGFQNRIINNTFLPMSDYNILFANEVDKHASEAFSLNFPQIPMLN
CEEEEEECCCCCCEEECHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHEECCCCCCCEEC
CSITELTEDYLDNHEIEYSNIDLVIGGPPCQSFSTVGRRQYDEKATMYKEYRRMLSFLQP
CHHHHHHHHHHCCCCEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KVFLFENVTGLLTMKNDEGEPVLEDIRKEFGDFSDFEIDLTYEIRETVLNAKHFGVPQNR
CEEEECCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCC
ERVFLLGIRKDLEIKQNWEFPERLLFEDDFLTLEDAIGDLPALRNGERKDAYDGDPYTPY
CEEEEEEECCCCCCCCCCCCHHHHHCCCCCEEHHHHHCCCCHHCCCCCCCCCCCCCCCHH
QNLMRNNSTILMDHFNGLNGEKMLRIMETVIPGEGKKYINELVESGQLDSKYYLTSGYNN
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCC
TYSKLWWDRPSSTITNNLSTPSSFRCIHPIQNRALTPREGARIQSFPDTFQFVGSKEKIN
CCHHHCCCCCCHHHHCCCCCCCCCEEECHHHCCCCCCCCCCCCCCCCHHHHHCCCHHHHH
SQIGNAVPPLLAIVIAQEIYNFFEENNWI
HHHCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRVVDLFSGAGGLTLGFQNRIINNTFLPMSDYNILFANEVDKHASEAFSLNFPQIPMLN
CEEEEEECCCCCCEEECHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHEECCCCCCCEEC
CSITELTEDYLDNHEIEYSNIDLVIGGPPCQSFSTVGRRQYDEKATMYKEYRRMLSFLQP
CHHHHHHHHHHCCCCEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KVFLFENVTGLLTMKNDEGEPVLEDIRKEFGDFSDFEIDLTYEIRETVLNAKHFGVPQNR
CEEEECCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCC
ERVFLLGIRKDLEIKQNWEFPERLLFEDDFLTLEDAIGDLPALRNGERKDAYDGDPYTPY
CEEEEEEECCCCCCCCCCCCHHHHHCCCCCEEHHHHHCCCCHHCCCCCCCCCCCCCCCHH
QNLMRNNSTILMDHFNGLNGEKMLRIMETVIPGEGKKYINELVESGQLDSKYYLTSGYNN
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCC
TYSKLWWDRPSSTITNNLSTPSSFRCIHPIQNRALTPREGARIQSFPDTFQFVGSKEKIN
CCHHHCCCCCCHHHHCCCCCCCCCEEECHHHCCCCCCCCCCCCCCCCHHHHHCCCHHHHH
SQIGNAVPPLLAIVIAQEIYNFFEENNWI
HHHCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2823226 [H]