Click here to switch to the map view.

The map label for this gene is catE [H]

Identifier: 226223270

GI number: 226223270

Start: 686501

End: 687340

Strand: Direct

Name: catE [H]

Synonym: Lm4b_00667

Alternate gene names: 226223270

Gene position: 686501-687340 (Clockwise)

Preceding gene: 226223269

Following gene: 226223272

Centisome position: 23.57

GC content: 40.95

Gene sequence:

>840_bases
ATGGCAGAAATTAGTGAAGAATTACGACTTGGCGAAGTTGTATTGAATGTTGGACATTTAAAAGAGATGGCAGGTTTTTA
TCAAGAAGTAATTGGTTTGAAGTTATTAGAGGAAAATGAGCGAATGGTTCGGCTCGGCGTGAGTGGGTCTGATGAAGCTT
TACTTGTTCTTAAAAAGATAGATAATGCGGTTGTTCCAGAAGTGCCGCGGATTGGCTTGTTTCATACCGCCTTTCTTTTG
CCAACGCGAGAAAGTTTAGCGGATGTGTTACTGCATTTGGCTAAATCAGGTTATCCGATTGATGGCGCGGGAGATCATGC
TTATAGCGAGGCGCTTTATTTACATGATATTGAAGGCAATGGCATTGAAATTTATGCAGACCGTCCAAAAGCAGAATGGA
TGCGTGATGGCGATGGCAATTTACCGATGGTGACAGAACAAGTAGATGTGGATTCGTTGCTTCAAATTGCGACCGATGAA
CCATTTACAGGGATGCCAAACGGAACGATAATTGGTCATGTGCATTTACAAGTGGCAGATGCTGACAAAGCGGAACAATT
TTATAAAGAGGTACTTGGAATGAACTTAACGACCGCTATTCCGTCAGCAAGGTTTTTTGCGGCTGGTGATTATCATCATC
ACATCGGTTCAAACGTCTGGGCAGGTCGCAACTTGGAACTTCTGCAAGAAAATGAAACGGGACTCGCTTGGTTTACAATT
ATTACTCCTGATAAAGATGCAATAATAACCAACTTAATAGAACAAGGATATGATGTGAAACGATTGGAAAATACTATTTC
CGTGACAGATTCTAACGGAATTAAGATTCACTTCAAATAA

Upstream 100 bases:

>100_bases
TTAAAAAATAATTAACTTACTTTTTGTAAGTCGTTTTATTGGAATTTGAACGCTCCTTATGTTGTAATGAATGTATTACA
ATAAAAGGAGCGTTTTTTAC

Downstream 100 bases:

>100_bases
AAAAAGAACTGTTTAAGCGAAAAACGAGCTTAAACAGTTTTTTGATGCGATAATAAGGGAATGGTAGATTAGACTAATTA
TTTTAATGTTTACGTTGCAT

Product: hypothetical protein

Products: NA

Alternate protein names: Catechol-induced ring cleavage extradiol dioxygenase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MAEISEELRLGEVVLNVGHLKEMAGFYQEVIGLKLLEENERMVRLGVSGSDEALLVLKKIDNAVVPEVPRIGLFHTAFLL
PTRESLADVLLHLAKSGYPIDGAGDHAYSEALYLHDIEGNGIEIYADRPKAEWMRDGDGNLPMVTEQVDVDSLLQIATDE
PFTGMPNGTIIGHVHLQVADADKAEQFYKEVLGMNLTTAIPSARFFAAGDYHHHIGSNVWAGRNLELLQENETGLAWFTI
ITPDKDAIITNLIEQGYDVKRLENTISVTDSNGIKIHFK

Sequences:

>Translated_279_residues
MAEISEELRLGEVVLNVGHLKEMAGFYQEVIGLKLLEENERMVRLGVSGSDEALLVLKKIDNAVVPEVPRIGLFHTAFLL
PTRESLADVLLHLAKSGYPIDGAGDHAYSEALYLHDIEGNGIEIYADRPKAEWMRDGDGNLPMVTEQVDVDSLLQIATDE
PFTGMPNGTIIGHVHLQVADADKAEQFYKEVLGMNLTTAIPSARFFAAGDYHHHIGSNVWAGRNLELLQENETGLAWFTI
ITPDKDAIITNLIEQGYDVKRLENTISVTDSNGIKIHFK
>Mature_278_residues
AEISEELRLGEVVLNVGHLKEMAGFYQEVIGLKLLEENERMVRLGVSGSDEALLVLKKIDNAVVPEVPRIGLFHTAFLLP
TRESLADVLLHLAKSGYPIDGAGDHAYSEALYLHDIEGNGIEIYADRPKAEWMRDGDGNLPMVTEQVDVDSLLQIATDEP
FTGMPNGTIIGHVHLQVADADKAEQFYKEVLGMNLTTAIPSARFFAAGDYHHHIGSNVWAGRNLELLQENETGLAWFTII
TPDKDAIITNLIEQGYDVKRLENTISVTDSNGIKIHFK

Specific function: Involved in the meta cleavage of catechol to 2- hydroxymuconic semialdehyde. Essential for growth and viability in the presence of catechol and probably involved in the detoxification of catechol [H]

COG id: COG2514

COG function: function code R; Predicted ring-cleavage extradiol dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the extradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR000486 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =1.13.11.2 [H]

Molecular weight: Translated: 30857; Mature: 30726

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEISEELRLGEVVLNVGHLKEMAGFYQEVIGLKLLEENERMVRLGVSGSDEALLVLKKI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCEEEEEEHH
DNAVVPEVPRIGLFHTAFLLPTRESLADVLLHLAKSGYPIDGAGDHAYSEALYLHDIEGN
CCCCCCCCCCCCHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCEEEEEEECCC
GIEIYADRPKAEWMRDGDGNLPMVTEQVDVDSLLQIATDEPFTGMPNGTIIGHVHLQVAD
CEEEEECCCHHHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEC
ADKAEQFYKEVLGMNLTTAIPSARFFAAGDYHHHIGSNVWAGRNLELLQENETGLAWFTI
CHHHHHHHHHHHCCCCHHHCCCCEEEEECCCHHHCCCCCCCCCCEEEEECCCCCEEEEEE
ITPDKDAIITNLIEQGYDVKRLENTISVTDSNGIKIHFK
ECCCHHHHHHHHHHCCCCHHHHCCEEEEECCCCEEEEEC
>Mature Secondary Structure 
AEISEELRLGEVVLNVGHLKEMAGFYQEVIGLKLLEENERMVRLGVSGSDEALLVLKKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCEEEEEEHH
DNAVVPEVPRIGLFHTAFLLPTRESLADVLLHLAKSGYPIDGAGDHAYSEALYLHDIEGN
CCCCCCCCCCCCHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCEEEEEEECCC
GIEIYADRPKAEWMRDGDGNLPMVTEQVDVDSLLQIATDEPFTGMPNGTIIGHVHLQVAD
CEEEEECCCHHHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEC
ADKAEQFYKEVLGMNLTTAIPSARFFAAGDYHHHIGSNVWAGRNLELLQENETGLAWFTI
CHHHHHHHHHHHCCCCHHHCCCCEEEEECCCHHHCCCCCCCCCCEEEEECCCCCEEEEEE
ITPDKDAIITNLIEQGYDVKRLENTISVTDSNGIKIHFK
ECCCHHHHHHHHHHCCCCHHHHCCEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8704981; 9384377 [H]