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The map label for this gene is iolD [H]

Identifier: 226223011

GI number: 226223011

Start: 411351

End: 413270

Strand: Direct

Name: iolD [H]

Synonym: Lm4b_00404

Alternate gene names: 226223011

Gene position: 411351-413270 (Clockwise)

Preceding gene: 226223010

Following gene: 226223012

Centisome position: 14.12

GC content: 46.09

Gene sequence:

>1920_bases
ATGGTGACTGAAAAAACGATTCGACTAACAACCGCACAAGCTTTGGTAAAATTTTTAAATCAGCAATATATCGAAGTGGA
CGGCGAAATGGCACCGTTTGTGGATGGTATTTTCACGCTGTTTGGACATGGAAATGTGGTTGGTATCGGGCAAGCCTTGG
AAGAAGCCCCAGGTCATTTAAAAGTGTATCAAGGGAAAAATGAGCAGGGCATGGCGCACGCGGCAATCGCTTATGCAAAA
CAAAAAAATCGCCAGCGCATCTACGCGTGTTCCACATCAGCAGGACCAGGTTCCGCGAACTTAATAACAGCAGCCGGCAC
CGCACTTGCAAACAATTTACCTGTGTTATTCCTGCCGGCCGACACATTCGCAACCAGACAGCCCGACCCAGTTTTGCAAC
AATTAGAGCATGAGTCGAGCACGGCAATTACAACCAATGATGGCTTCCAAGCAGTCTCCAGATATTTTGACCGTGTGCAG
CGACCAGAACAACTCATGAGCGCCCTGATTCGCGCTTTCGAAGTAATGACCAACCCAGCGAGCGCGGGACCAGCAACAAT
CTGCATTGCGCAAGACACAGAAGGAGAAGCATTCGATTATCCAGTCGAATTTTTTCAAAAACGAATTCACTATCTTAATC
GGCAAATCCCGACAAAGCGTGAACTAACCGAAGCAGCGCGACTCATCCAAGCGAGCAAAACCCCAGTTATCATCGTCGGA
GGTGGCGCCCGCTATTCAAGAGCACGCGAAGAATTAATCGCCCTTTCCGAGCAAACCAACATTCCGCTCGTCGAAACACA
TGCCGGGAAATCAACGCTTGAGTTTGACTTCAAAAACAACCTAGGTGGCACGGGAATCCTCGGGACGCTTGCTGCCAATA
AAGCTATCCGTGACGCCGATTTAGTCATCGGAATTGGCACGCGCTATACCGATTTCACAACCAGTTCCAAAACGGCATTC
GATCCAACAACCAAATTTATCAATATCAATGTCAGCCGCATGCAAACATACAAATTAGATGCCTTCCAAGTGGTCGGTGA
CGCTAGAGCAACCCTAGCTGAACTCGCACCACTTTTACAAGATTATCAGACTCAATTTGGAAACAAGATAGCCGCATACA
AAACCGAGTGGCTAGACGAAAGAGCACGGCTACAAACCACCAAATTCAACCGAGAAGCATTTACGCCAGAAATCAAAGAT
CAATTCGACCAAGCGACCTTAAACGAATATGCAGACCGCTTGCAGACCGAATTTACGCAAACAGAAGCACTTATCACAAT
TAATGACAACGTGGCACCGGATAGCATTGTCGTTTGTTCGGCCGGTTCACTTCCCGGAGATTTGCAACGACTCTGGAACC
CAGCCGTACCAGACACCTATCACTTAGAATATGGCTATTCCTGCATGGGCTACGAAATAAACGGTGCTCTCGGCGCGAAA
ATGGCCGCAGCAAAAAAACAAGAAGTATACGCCATCGTTGGCGACGGCAGCTTCTGCATGTCACACTCAGAACTACTAAC
ATCCTTACAATACGGCAAAAAAATTAACATTATGCTCTTCGATAATTCCGGTTTTGGCTGCATTAACAACCTCCAAATGG
CAAACGGCAGCGACAGTTTTTTCTGCGAATTCCGCGATAGCGACAACCAAATCATGCAAGTCGATTACGCCAAAATTGCA
GAAGGATACGGAGCAAAAGTCTATAGAGCCAACACGAAAGAAGATTTAATCAGCGCACTGGAAGACGCAAAAACACAAAG
CAAAACTACTTTGATCGAAATGAAAGTACTACCAAAAACTATGTCAGAAGGCTATCTTAATTGGTGGAATGTGGGAGTTT
CCGAAGTTTCAAACAAAGAAAGTATCAAGCAAGCATACGAAGAAAAACAAGCAAACCTGAAAAATGCCCGACTATATTAA

Upstream 100 bases:

>100_bases
CGCGGCTTCGATTGTTGTTAGCAAGCATAGTTCCTCAGACGCGATGCCAACAGCTGATGAAATCAAAGCGCTCATTGCGC
AAGCGGAATAGGAGGAGATT

Downstream 100 bases:

>100_bases
GCAAAACAAAAACAAGTCCATCACCGGACTTGTTTTTGTTTTGCCACTTCAATAGCCCCGCCTTGCATCACACCAGTTTT
TCTTTATAATAAAAAGTAAC

Product: IolD protein, to acetolactate synthase

Products: NA

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MVTEKTIRLTTAQALVKFLNQQYIEVDGEMAPFVDGIFTLFGHGNVVGIGQALEEAPGHLKVYQGKNEQGMAHAAIAYAK
QKNRQRIYACSTSAGPGSANLITAAGTALANNLPVLFLPADTFATRQPDPVLQQLEHESSTAITTNDGFQAVSRYFDRVQ
RPEQLMSALIRAFEVMTNPASAGPATICIAQDTEGEAFDYPVEFFQKRIHYLNRQIPTKRELTEAARLIQASKTPVIIVG
GGARYSRAREELIALSEQTNIPLVETHAGKSTLEFDFKNNLGGTGILGTLAANKAIRDADLVIGIGTRYTDFTTSSKTAF
DPTTKFININVSRMQTYKLDAFQVVGDARATLAELAPLLQDYQTQFGNKIAAYKTEWLDERARLQTTKFNREAFTPEIKD
QFDQATLNEYADRLQTEFTQTEALITINDNVAPDSIVVCSAGSLPGDLQRLWNPAVPDTYHLEYGYSCMGYEINGALGAK
MAAAKKQEVYAIVGDGSFCMSHSELLTSLQYGKKINIMLFDNSGFGCINNLQMANGSDSFFCEFRDSDNQIMQVDYAKIA
EGYGAKVYRANTKEDLISALEDAKTQSKTTLIEMKVLPKTMSEGYLNWWNVGVSEVSNKESIKQAYEEKQANLKNARLY

Sequences:

>Translated_639_residues
MVTEKTIRLTTAQALVKFLNQQYIEVDGEMAPFVDGIFTLFGHGNVVGIGQALEEAPGHLKVYQGKNEQGMAHAAIAYAK
QKNRQRIYACSTSAGPGSANLITAAGTALANNLPVLFLPADTFATRQPDPVLQQLEHESSTAITTNDGFQAVSRYFDRVQ
RPEQLMSALIRAFEVMTNPASAGPATICIAQDTEGEAFDYPVEFFQKRIHYLNRQIPTKRELTEAARLIQASKTPVIIVG
GGARYSRAREELIALSEQTNIPLVETHAGKSTLEFDFKNNLGGTGILGTLAANKAIRDADLVIGIGTRYTDFTTSSKTAF
DPTTKFININVSRMQTYKLDAFQVVGDARATLAELAPLLQDYQTQFGNKIAAYKTEWLDERARLQTTKFNREAFTPEIKD
QFDQATLNEYADRLQTEFTQTEALITINDNVAPDSIVVCSAGSLPGDLQRLWNPAVPDTYHLEYGYSCMGYEINGALGAK
MAAAKKQEVYAIVGDGSFCMSHSELLTSLQYGKKINIMLFDNSGFGCINNLQMANGSDSFFCEFRDSDNQIMQVDYAKIA
EGYGAKVYRANTKEDLISALEDAKTQSKTTLIEMKVLPKTMSEGYLNWWNVGVSEVSNKESIKQAYEEKQANLKNARLY
>Mature_639_residues
MVTEKTIRLTTAQALVKFLNQQYIEVDGEMAPFVDGIFTLFGHGNVVGIGQALEEAPGHLKVYQGKNEQGMAHAAIAYAK
QKNRQRIYACSTSAGPGSANLITAAGTALANNLPVLFLPADTFATRQPDPVLQQLEHESSTAITTNDGFQAVSRYFDRVQ
RPEQLMSALIRAFEVMTNPASAGPATICIAQDTEGEAFDYPVEFFQKRIHYLNRQIPTKRELTEAARLIQASKTPVIIVG
GGARYSRAREELIALSEQTNIPLVETHAGKSTLEFDFKNNLGGTGILGTLAANKAIRDADLVIGIGTRYTDFTTSSKTAF
DPTTKFININVSRMQTYKLDAFQVVGDARATLAELAPLLQDYQTQFGNKIAAYKTEWLDERARLQTTKFNREAFTPEIKD
QFDQATLNEYADRLQTEFTQTEALITINDNVAPDSIVVCSAGSLPGDLQRLWNPAVPDTYHLEYGYSCMGYEINGALGAK
MAAAKKQEVYAIVGDGSFCMSHSELLTSLQYGKKINIMLFDNSGFGCINNLQMANGSDSFFCEFRDSDNQIMQVDYAKIA
EGYGAKVYRANTKEDLISALEDAKTQSKTTLIEMKVLPKTMSEGYLNWWNVGVSEVSNKESIKQAYEEKQANLKNARLY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=575, Percent_Identity=25.2173913043478, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1790104, Length=597, Percent_Identity=22.7805695142379, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1786717, Length=568, Percent_Identity=23.0633802816901, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787096, Length=528, Percent_Identity=22.5378787878788, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788716, Length=543, Percent_Identity=22.0994475138122, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17531299, Length=320, Percent_Identity=26.25, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17531301, Length=320, Percent_Identity=26.25, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323755, Length=561, Percent_Identity=24.777183600713, Blast_Score=130, Evalue=7e-31,
Organism=Drosophila melanogaster, GI19922626, Length=610, Percent_Identity=22.1311475409836, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.-.-.-

Molecular weight: Translated: 70586; Mature: 70586

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTEKTIRLTTAQALVKFLNQQYIEVDGEMAPFVDGIFTLFGHGNVVGIGQALEEAPGHL
CCCCCEEEHHHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHCCCCEEECCHHHHHCCCCE
KVYQGKNEQGMAHAAIAYAKQKNRQRIYACSTSAGPGSANLITAAGTALANNLPVLFLPA
EEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCHHHHCCCCEEEECC
DTFATRQPDPVLQQLEHESSTAITTNDGFQAVSRYFDRVQRPEQLMSALIRAFEVMTNPA
CCCCCCCCCHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
SAGPATICIAQDTEGEAFDYPVEFFQKRIHYLNRQIPTKRELTEAARLIQASKTPVIIVG
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEE
GGARYSRAREELIALSEQTNIPLVETHAGKSTLEFDFKNNLGGTGILGTLAANKAIRDAD
CCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCC
LVIGIGTRYTDFTTSSKTAFDPTTKFININVSRMQTYKLDAFQVVGDARATLAELAPLLQ
EEEECCCCEEECCCCCCCCCCCCCEEEEEEHHHEEEEECCHHEEHHHHHHHHHHHHHHHH
DYQTQFGNKIAAYKTEWLDERARLQTTKFNREAFTPEIKDQFDQATLNEYADRLQTEFTQ
HHHHHHCCEEEHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
TEALITINDNVAPDSIVVCSAGSLPGDLQRLWNPAVPDTYHLEYGYSCMGYEINGALGAK
CEEEEEECCCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEEECCCEEEEEEECCCCCHH
MAAAKKQEVYAIVGDGSFCMSHSELLTSLQYGKKINIMLFDNSGFGCINNLQMANGSDSF
HHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEECCCCCCE
FCEFRDSDNQIMQVDYAKIAEGYGAKVYRANTKEDLISALEDAKTQSKTTLIEMKVLPKT
EEEEECCCCCEEEEEHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECHHH
MSEGYLNWWNVGVSEVSNKESIKQAYEEKQANLKNARLY
HHCCCCEEECCCHHHCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVTEKTIRLTTAQALVKFLNQQYIEVDGEMAPFVDGIFTLFGHGNVVGIGQALEEAPGHL
CCCCCEEEHHHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHCCCCEEECCHHHHHCCCCE
KVYQGKNEQGMAHAAIAYAKQKNRQRIYACSTSAGPGSANLITAAGTALANNLPVLFLPA
EEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCHHHHCCCCEEEECC
DTFATRQPDPVLQQLEHESSTAITTNDGFQAVSRYFDRVQRPEQLMSALIRAFEVMTNPA
CCCCCCCCCHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
SAGPATICIAQDTEGEAFDYPVEFFQKRIHYLNRQIPTKRELTEAARLIQASKTPVIIVG
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEE
GGARYSRAREELIALSEQTNIPLVETHAGKSTLEFDFKNNLGGTGILGTLAANKAIRDAD
CCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCC
LVIGIGTRYTDFTTSSKTAFDPTTKFININVSRMQTYKLDAFQVVGDARATLAELAPLLQ
EEEECCCCEEECCCCCCCCCCCCCEEEEEEHHHEEEEECCHHEEHHHHHHHHHHHHHHHH
DYQTQFGNKIAAYKTEWLDERARLQTTKFNREAFTPEIKDQFDQATLNEYADRLQTEFTQ
HHHHHHCCEEEHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
TEALITINDNVAPDSIVVCSAGSLPGDLQRLWNPAVPDTYHLEYGYSCMGYEINGALGAK
CEEEEEECCCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEEECCCEEEEEEECCCCCHH
MAAAKKQEVYAIVGDGSFCMSHSELLTSLQYGKKINIMLFDNSGFGCINNLQMANGSDSF
HHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEECCCCCCE
FCEFRDSDNQIMQVDYAKIAEGYGAKVYRANTKEDLISALEDAKTQSKTTLIEMKVLPKT
EEEEECCCCCEEEEEHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECHHH
MSEGYLNWWNVGVSEVSNKESIKQAYEEKQANLKNARLY
HHCCCCEEECCCHHHCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]