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The map label for this gene is gmuD [H]

Identifier: 226222999

GI number: 226222999

Start: 400158

End: 401600

Strand: Direct

Name: gmuD [H]

Synonym: Lm4b_00391

Alternate gene names: 226222999

Gene position: 400158-401600 (Clockwise)

Preceding gene: 226222997

Following gene: 226223000

Centisome position: 13.74

GC content: 38.6

Gene sequence:

>1443_bases
ATGAAAGAATTATTAACGTTTCCAAAAGATTTTTGGTGGGGATCGGCGTGGTCTGCGGAACAAGCAGAAGGTCGCGGCGA
CACTGGTAAGGCAAAAACTGTTTGGGAGCATTGGTTTGAAACAGAACCGAATCGCTTTTACGAAGGCGTAGGTTCAGAAA
TTACAACAGATCATTTTAATCGTTATAAAGAAGACGTACAGTGGATGAAGAAAACAGGACAAAATTCGTTCCGGATTTCG
ATTTCATGGGCGCGAATGTTTCCTAATGATGGCGTTGGCGAAGTGAACCCGAAAGCTATTGCGTTTTATCGCGATTTACT
TACTGAAATGAATGAAAACGGTATCAAACCATTCGCCAACTTATATCATTTCGATATGCCCGTTGCACTTCAAGATGCTG
GTGGCTGGGAATCAAGAGAAGTTGTAGATGCCTATGTTCATTTTGCAGATACTTGTTTTAAAGAATTTGGCGATTTAGTT
TATCACTGGTTTACGTTTAACGAACCACTAGGACCGATTCTTGGCGGCTATTTAGAAGATTTCCATTATCCGAATCAAAT
CGACTTCAAACGCGGTGCACAAGCTAGTTTTAATACGATTTTGGCGCATGCGTTAGCTATTAAAGCATTTAAAAAATTAA
ACTTGTCATCAAAAATCGGCGTTATTTTAAATCTTAGCCCGACGTATCCACGTAGCCAAAACGAATACGATTTAGAAGCT
GCGGAAATTTGTGATGCTTTTTACACGAGAAGCTTCTTGGATCCGATGGTTAAAGGAACTTTCCCAGAAAAATTAGTTGA
AATTATGCGCGAATACGATCAAATGCCGGAATACACGGAAGAAGATTTGACGATTATTAAAGAAAATACGGCGCAAATTC
TTGGCCTAAATTACTATGAACCAAGACGCGTCAAAGCCCGTTTAACAGCAATTAATGAAAAAAGTCCGTTTTTACCAGAT
TGGTTTTTTGAACCGCATAATATGCCAGGTAAACGAATGAATATTTACCGCGGTTGGGAAATTTATGAACGTGGGATTTA
CGATTTATGTATTGATATTCGTGATAATTACGGCAATATCGAATCATTTATTTCTGAAAATGGTATGGGTGTGGCAAATG
AAGAGCGTTTCTTAAATGAAGAAGGACAAATTCAAGATGATTACCGGATTCAATTTGTCAAAGATCATTTGGCTTATGTA
CATCAAGCGATTGCAGAAGGTTGCGATATTAAAGGGTATCATCTGTGGACATTCATTGATTGTTGGTCGTGGATTAACGC
ATATAAAAACCGTTACGGACTCGTTTCACTTGATGTCGAAACTGGCAAACGCACAATGAAGAAAAGTGGCGAACTTTATA
AAAAAATGAGTGACATGAACGGTTTTGAATATGATACAAGCAAACTAGTTGGGACGAAAAGGGAGGAAACAACTAATGGC
TGA

Upstream 100 bases:

>100_bases
AGGTATCCCTATAAACAAAAGTATATATCTTTTAATAAAAGTATTGTTTTTTAAATAAATGTATGCTATATTTATTTTGA
CAAAAGGGAGGCCATAAAAA

Downstream 100 bases:

>100_bases
AACGAAAAAGAAGTCGATTGTTAATGGATTTATTAATGTAGCACAACGTCTTGGTGGTCAGATTCATTTGCGTTCATTGC
GTGATGCTTTTGCGAGTATT

Product: beta-glucosidase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MKELLTFPKDFWWGSAWSAEQAEGRGDTGKAKTVWEHWFETEPNRFYEGVGSEITTDHFNRYKEDVQWMKKTGQNSFRIS
ISWARMFPNDGVGEVNPKAIAFYRDLLTEMNENGIKPFANLYHFDMPVALQDAGGWESREVVDAYVHFADTCFKEFGDLV
YHWFTFNEPLGPILGGYLEDFHYPNQIDFKRGAQASFNTILAHALAIKAFKKLNLSSKIGVILNLSPTYPRSQNEYDLEA
AEICDAFYTRSFLDPMVKGTFPEKLVEIMREYDQMPEYTEEDLTIIKENTAQILGLNYYEPRRVKARLTAINEKSPFLPD
WFFEPHNMPGKRMNIYRGWEIYERGIYDLCIDIRDNYGNIESFISENGMGVANEERFLNEEGQIQDDYRIQFVKDHLAYV
HQAIAEGCDIKGYHLWTFIDCWSWINAYKNRYGLVSLDVETGKRTMKKSGELYKKMSDMNGFEYDTSKLVGTKREETTNG

Sequences:

>Translated_480_residues
MKELLTFPKDFWWGSAWSAEQAEGRGDTGKAKTVWEHWFETEPNRFYEGVGSEITTDHFNRYKEDVQWMKKTGQNSFRIS
ISWARMFPNDGVGEVNPKAIAFYRDLLTEMNENGIKPFANLYHFDMPVALQDAGGWESREVVDAYVHFADTCFKEFGDLV
YHWFTFNEPLGPILGGYLEDFHYPNQIDFKRGAQASFNTILAHALAIKAFKKLNLSSKIGVILNLSPTYPRSQNEYDLEA
AEICDAFYTRSFLDPMVKGTFPEKLVEIMREYDQMPEYTEEDLTIIKENTAQILGLNYYEPRRVKARLTAINEKSPFLPD
WFFEPHNMPGKRMNIYRGWEIYERGIYDLCIDIRDNYGNIESFISENGMGVANEERFLNEEGQIQDDYRIQFVKDHLAYV
HQAIAEGCDIKGYHLWTFIDCWSWINAYKNRYGLVSLDVETGKRTMKKSGELYKKMSDMNGFEYDTSKLVGTKREETTNG
>Mature_480_residues
MKELLTFPKDFWWGSAWSAEQAEGRGDTGKAKTVWEHWFETEPNRFYEGVGSEITTDHFNRYKEDVQWMKKTGQNSFRIS
ISWARMFPNDGVGEVNPKAIAFYRDLLTEMNENGIKPFANLYHFDMPVALQDAGGWESREVVDAYVHFADTCFKEFGDLV
YHWFTFNEPLGPILGGYLEDFHYPNQIDFKRGAQASFNTILAHALAIKAFKKLNLSSKIGVILNLSPTYPRSQNEYDLEA
AEICDAFYTRSFLDPMVKGTFPEKLVEIMREYDQMPEYTEEDLTIIKENTAQILGLNYYEPRRVKARLTAINEKSPFLPD
WFFEPHNMPGKRMNIYRGWEIYERGIYDLCIDIRDNYGNIESFISENGMGVANEERFLNEEGQIQDDYRIQFVKDHLAYV
HQAIAEGCDIKGYHLWTFIDCWSWINAYKNRYGLVSLDVETGKRTMKKSGELYKKMSDMNGFEYDTSKLVGTKREETTNG

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=486, Percent_Identity=29.2181069958848, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI13273313, Length=481, Percent_Identity=28.2744282744283, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI110681710, Length=481, Percent_Identity=29.3139293139293, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI28376633, Length=485, Percent_Identity=26.3917525773196, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI24497614, Length=489, Percent_Identity=25.9713701431493, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI1789070, Length=488, Percent_Identity=31.7622950819672, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI2367174, Length=490, Percent_Identity=31.4285714285714, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI2367270, Length=475, Percent_Identity=30.9473684210526, Blast_Score=182, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17552856, Length=478, Percent_Identity=30.9623430962343, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17539390, Length=468, Percent_Identity=30.5555555555556, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI21356577, Length=487, Percent_Identity=25.8726899383984, Blast_Score=182, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55962; Mature: 55962

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELLTFPKDFWWGSAWSAEQAEGRGDTGKAKTVWEHWFETEPNRFYEGVGSEITTDHFN
CCCHHCCCHHHCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCHHHHH
RYKEDVQWMKKTGQNSFRISISWARMFPNDGVGEVNPKAIAFYRDLLTEMNENGIKPFAN
HHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
LYHFDMPVALQDAGGWESREVVDAYVHFADTCFKEFGDLVYHWFTFNEPLGPILGGYLED
HHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHH
FHYPNQIDFKRGAQASFNTILAHALAIKAFKKLNLSSKIGVILNLSPTYPRSQNEYDLEA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHH
AEICDAFYTRSFLDPMVKGTFPEKLVEIMREYDQMPEYTEEDLTIIKENTAQILGLNYYE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCCC
PRRVKARLTAINEKSPFLPDWFFEPHNMPGKRMNIYRGWEIYERGIYDLCIDIRDNYGNI
CHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHCCCEEEEEEECCCCCHH
ESFISENGMGVANEERFLNEEGQIQDDYRIQFVKDHLAYVHQAIAEGCDIKGYHLWTFID
HHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
CWSWINAYKNRYGLVSLDVETGKRTMKKSGELYKKMSDMNGFEYDTSKLVGTKREETTNG
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKELLTFPKDFWWGSAWSAEQAEGRGDTGKAKTVWEHWFETEPNRFYEGVGSEITTDHFN
CCCHHCCCHHHCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCHHHHH
RYKEDVQWMKKTGQNSFRISISWARMFPNDGVGEVNPKAIAFYRDLLTEMNENGIKPFAN
HHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
LYHFDMPVALQDAGGWESREVVDAYVHFADTCFKEFGDLVYHWFTFNEPLGPILGGYLED
HHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHH
FHYPNQIDFKRGAQASFNTILAHALAIKAFKKLNLSSKIGVILNLSPTYPRSQNEYDLEA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHH
AEICDAFYTRSFLDPMVKGTFPEKLVEIMREYDQMPEYTEEDLTIIKENTAQILGLNYYE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCCC
PRRVKARLTAINEKSPFLPDWFFEPHNMPGKRMNIYRGWEIYERGIYDLCIDIRDNYGNI
CHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHCCCEEEEEEECCCCCHH
ESFISENGMGVANEERFLNEEGQIQDDYRIQFVKDHLAYVHQAIAEGCDIKGYHLWTFID
HHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
CWSWINAYKNRYGLVSLDVETGKRTMKKSGELYKKMSDMNGFEYDTSKLVGTKREETTNG
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]