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The map label for this gene is ltaA [H]

Identifier: 226222940

GI number: 226222940

Start: 336448

End: 337536

Strand: Direct

Name: ltaA [H]

Synonym: Lm4b_00332

Alternate gene names: 226222940

Gene position: 336448-337536 (Clockwise)

Preceding gene: 226222939

Following gene: 226222941

Centisome position: 11.55

GC content: 43.99

Gene sequence:

>1089_bases
ATGATAAACACACTAAAAACCAGCTACCAAAAAACACCATATAAACTAGGCGGGAACGGTCCGCGCAATGTAGGTGTTCT
GACAGAAGCACTTCAAAATATAGATGATAACCTAGAAAGCGATATTTACGGAAATGGCGCAGTTATTGAAAACTTTGAAA
CAAAAATCGCGAAAATTCTCGGGAAACAATCTGCGGTATTTTTTCCAAGTGGGACGATGGCTCAGCAAATCGCGTTAAGG
ATTTGGGCTGACCGGAAAGAGAATCGGTGCGTGGCATATCATCCGCTCTCTCATTTGGAGATTCACGAGCAAGATGGGTT
AAAAGAATTGCAACAAATCACGCCCATATTACTTGGAACAGCGAATCGACTTTTGACAATTGACGATATTAAAAGCCTTC
GCGAGCCAGTTTCTAGTGTGCTTATCGAACTTCCACAACGAGAAATTGGTGGGCAGCTGCCTGCTTTTGAAGAGCTTGAG
GAAATTTCGGAGTATTGCCATGAACAGGGTATTTCGTTGCATCTTGACGGGGCACGGTTATGGGAAATCACGCCGTTTTA
TCAGAAGTCTGCGGAAGAAATTTGTGCGCTGTTTGATAGTGTGTATGTGTCGTTTTACAAAGGAATTGGCGGGATTGCTG
GGGCGATTCTGGCTGGGAATGATGATTTTGTGCAAGAGGCGAAAATCTGGAAACGACGGTATGGCGGAGATTTGATTAGT
CTGTATCCGTATATTTTGTCCGCGGATTACTATTTTGAAAAACGGATTGGGAAAATGGCGGAATATTTTGAAGCGGCGAA
AGGATTGGCTGAGCGATTTAATTCGTGTTCGGGCGTGAAGACTGTGCCGGAAGTGCCGGTTTCTAATATGTTTCATGTTT
ATTTTGAGAAGTCGGCTGATGAGATTGGGGCGATTTTGACGAAGATTCAGGATGAAACTGGGGTTGGGATTTCGGGGTAT
TTGCAGGAGAAATCGGCGGATGTTTGTGCGTTTGAGGTTTCGGTTGGGGATGCGTTTGCGGAAATACCGGTGGAGATTTT
GGATGTTGTGTTTAAACAATTAGAGGTGGATTTACCATGCCAAAACTAA

Upstream 100 bases:

>100_bases
GATAGTTTTTTTGTTACCGTAGTTAAATTTACAATAGGCTTGAGTTCTAGGTATAATATAGATAAATTTAGAAATTATAT
CGTAAAAGGAGTGAACCAAA

Downstream 100 bases:

>100_bases
TCATCGACGCAGACGATTTCGGTCTATCCAAAGCCATCAACCACGGCATCATCGAAAGCTACAAAACCGGCATCACAACA
TCCACTTTACTCATGCCGAA

Product: low specificity L-allo-threonine aldolase

Products: Acetaldehyde; Glycine [C]

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MINTLKTSYQKTPYKLGGNGPRNVGVLTEALQNIDDNLESDIYGNGAVIENFETKIAKILGKQSAVFFPSGTMAQQIALR
IWADRKENRCVAYHPLSHLEIHEQDGLKELQQITPILLGTANRLLTIDDIKSLREPVSSVLIELPQREIGGQLPAFEELE
EISEYCHEQGISLHLDGARLWEITPFYQKSAEEICALFDSVYVSFYKGIGGIAGAILAGNDDFVQEAKIWKRRYGGDLIS
LYPYILSADYYFEKRIGKMAEYFEAAKGLAERFNSCSGVKTVPEVPVSNMFHVYFEKSADEIGAILTKIQDETGVGISGY
LQEKSADVCAFEVSVGDAFAEIPVEILDVVFKQLEVDLPCQN

Sequences:

>Translated_362_residues
MINTLKTSYQKTPYKLGGNGPRNVGVLTEALQNIDDNLESDIYGNGAVIENFETKIAKILGKQSAVFFPSGTMAQQIALR
IWADRKENRCVAYHPLSHLEIHEQDGLKELQQITPILLGTANRLLTIDDIKSLREPVSSVLIELPQREIGGQLPAFEELE
EISEYCHEQGISLHLDGARLWEITPFYQKSAEEICALFDSVYVSFYKGIGGIAGAILAGNDDFVQEAKIWKRRYGGDLIS
LYPYILSADYYFEKRIGKMAEYFEAAKGLAERFNSCSGVKTVPEVPVSNMFHVYFEKSADEIGAILTKIQDETGVGISGY
LQEKSADVCAFEVSVGDAFAEIPVEILDVVFKQLEVDLPCQN
>Mature_362_residues
MINTLKTSYQKTPYKLGGNGPRNVGVLTEALQNIDDNLESDIYGNGAVIENFETKIAKILGKQSAVFFPSGTMAQQIALR
IWADRKENRCVAYHPLSHLEIHEQDGLKELQQITPILLGTANRLLTIDDIKSLREPVSSVLIELPQREIGGQLPAFEELE
EISEYCHEQGISLHLDGARLWEITPFYQKSAEEICALFDSVYVSFYKGIGGIAGAILAGNDDFVQEAKIWKRRYGGDLIS
LYPYILSADYYFEKRIGKMAEYFEAAKGLAERFNSCSGVKTVPEVPVSNMFHVYFEKSADEIGAILTKIQDETGVGISGY
LQEKSADVCAFEVSVGDAFAEIPVEILDVVFKQLEVDLPCQN

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=239, Percent_Identity=27.1966527196653, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193206520, Length=204, Percent_Identity=25.4901960784314, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI193206524, Length=204, Percent_Identity=25.4901960784314, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI193206522, Length=204, Percent_Identity=25.4901960784314, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320789, Length=245, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21355439, Length=213, Percent_Identity=27.6995305164319, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5 [C]

Molecular weight: Translated: 40296; Mature: 40296

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINTLKTSYQKTPYKLGGNGPRNVGVLTEALQNIDDNLESDIYGNGAVIENFETKIAKIL
CCCCCCHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
GKQSAVFFPSGTMAQQIALRIWADRKENRCVAYHPLSHLEIHEQDGLKELQQITPILLGT
CCCCEEECCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHCC
ANRLLTIDDIKSLREPVSSVLIELPQREIGGQLPAFEELEEISEYCHEQGISLHLDGARL
CCCEEEHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCEE
WEITPFYQKSAEEICALFDSVYVSFYKGIGGIAGAILAGNDDFVQEAKIWKRRYGGDLIS
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHCCCHHH
LYPYILSADYYFEKRIGKMAEYFEAAKGLAERFNSCSGVKTVPEVPVSNMFHVYFEKSAD
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHCCHH
EIGAILTKIQDETGVGISGYLQEKSADVCAFEVSVGDAFAEIPVEILDVVFKQLEVDLPC
HHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEECCHHHHHCCHHHHHHHHHHHCCCCCC
QN
CC
>Mature Secondary Structure
MINTLKTSYQKTPYKLGGNGPRNVGVLTEALQNIDDNLESDIYGNGAVIENFETKIAKIL
CCCCCCHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
GKQSAVFFPSGTMAQQIALRIWADRKENRCVAYHPLSHLEIHEQDGLKELQQITPILLGT
CCCCEEECCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHCC
ANRLLTIDDIKSLREPVSSVLIELPQREIGGQLPAFEELEEISEYCHEQGISLHLDGARL
CCCEEEHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCEE
WEITPFYQKSAEEICALFDSVYVSFYKGIGGIAGAILAGNDDFVQEAKIWKRRYGGDLIS
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHCCCHHH
LYPYILSADYYFEKRIGKMAEYFEAAKGLAERFNSCSGVKTVPEVPVSNMFHVYFEKSAD
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHCCHH
EIGAILTKIQDETGVGISGYLQEKSADVCAFEVSVGDAFAEIPVEILDVVFKQLEVDLPC
HHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEECCHHHHHCCHHHHHHHHHHHCCCCCC
QN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 0.22 {L-allo-Thr}} 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} [C]

Substrates: L-Threonine [C]

Specific reaction: L-Threonine <==> Acetaldehyde + Glycine [C]

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]